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产品概述
名称 | NCI-H446 [H446] (人小细胞肺癌细胞) (STR鉴定正确) |
别称 | H446; H-446; NCI-446; NCIH446 |
种属 | 人 |
生长特性 | 半贴半悬 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
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推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | NCI-H446细胞是从一位小细胞肺癌患者的胸水中建立的,NCI-H446细胞的原始形态并不具有小细胞肺癌特征。NCI-H446细胞是小细胞肺癌的生化和形态学上的变种,表达神经元特有的烯醇酶和脑部肌酸激酶同功酶。NCI-H446细胞内左旋多巴脱羧酶、蚕素、抗利尿激素、催产素或胃泌激素释放肽未达到可检测水平。C-myc DNA序列扩增约20倍,c-myc RNA比正常细胞增加15倍。最初,传代培养基用RPMI-1640(含5%胎牛血清、10nM氢化可的松、0.005mg/ml胰岛素、0.01mg/ml铁传递蛋白、10nM 17-β-雌二醇、30nM亚硒酸钠)。 |
年龄(性别) | 男性;61岁 |
组织来源 | 肺;转移灶:肋膜渗出癌;小细胞肺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in nude mice (The cells form transplantable tumors with non-typical SCLC histology). |
保藏机构 | ATCC; HTB-171 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 13 D2S1338 18,20 D3S1358 17 D5S818 11 D7S820 10,11 D8S1179 13,15 D13S317 8 D16S539 12 D18S51 12,13 D19S433 13,14 D21S11 28 FGA 22 PentaD 12,13 PentaE 9,10 TH01 8,9.3 TPOX 9,11 vWA 18,19 D6S1043 11 D12S391 17,18 D2S441 10,11 -
STR鉴定图
参考文献
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Dynamic phenotypic reprogramming and chemoresistance induced by lung fibroblasts in small cell lung cancer (2024-02-05)
作者:Yuanhua Lu:1.Postdoctoral Research Workstation, Jilin Cancer Hospital, Changchun, China. ; Hui Li:1.Medical Oncology Translational Research Lab, Jilin Cancer Hospital, No. 1066, Jinhu Road, High-tech District, Changchun, 130012, Jilin, China. ; Peiyan Zhao:1.Medical Oncology Translational Research Lab, Jilin Cancer Hospital, No. 1066, Jinhu Road, High-tech District, Changchun, 130012, Jilin, China. ; Lin Tian:1.Postdoctoral Research Workstation, Jilin Cancer Hospital, Changchun, China. ; Yan Liu:1.Medical Oncology Translational Research Lab, Jilin Cancer Hospital, No. 1066, Jinhu Road, High-tech District, Changchun, 130012, Jilin, China. ; XiaoDan Sun:1.Department of 1st Gynecologic Oncology Surgery, Jilin Cancer Hospital, Changchun, China. ; Ying Cheng:1.Medical Oncology Translational Research Lab, Jilin Cancer Hospital, No. 1066, Jinhu Road, High-tech District, Changchun, 130012, Jilin, China. chengying@csco.org.cn. ; 2.Department of Medical Thoracic Oncology, Jilin Cancer Hosp
期刊:Dynamic phenotypic reprogramming and chemoresistance induced by lung fibroblasts in small cell lung cancer
DOI:10.1038/s41598-024-52687-z
影响因子 :4.6
引用产品: NCI-H69 细胞 , NCI-H2227 细胞 , H1092 细胞 , NCI-H446 [H446] 细胞 , 21H 细胞 , NCI-H196 细胞 , SBC-5 细胞 , HFL1 细胞
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Design, synthesis and biological evaluation of novel podophyllotoxin derivatives as tubulin-targeting anticancer agents (2024-02-23)
作者:Yujin Guo:1.Institute of Clinical Pharmacy, Jining First People's Hospital, Shandong First Medical University, Jining, P.R. China. ; 2.Translational Pharmaceutical Laboratory, Jining First People's Hospital, Shandong First Medical University, Jining, P.R. China. ; Beibei Chen:1.Translational Pharmaceutical Laboratory, Jining First People's Hospital, Shandong First Medical University, Jining, P.R. China. ; Jinxiu Guo:1.Translational Pharmaceutical Laboratory, Jining First People's Hospital, Shandong First Medical University, Jining, P.R. China. ; Pei Jiang:1.Institute of Clinical Pharmacy, Jining First People's Hospital, Shandong First Medical University, Jining, P.R. China. ; 2.Translational Pharmaceutical Laboratory, Jining First People's Hospital, Shandong First Medical University, Jining, P.R. China. ; Jianhua Wang:1.Translational Pharmaceutical Laboratory, Jining First People's Hospital, Shandong First Medical University, Jining, P.R. China. ; 2.Postdoctoral of Shandong Univers
期刊:Design, synthesis and biological evaluation of novel podophyllotoxin derivatives as tubulin-targeting anticancer agents
DOI:10.1080/13880209.2024.2318350
影响因子 :3.8
引用产品: NCI-H446 [H446] 细胞 , MCF7 [MCF-7] 细胞 , Hela 细胞 , A549 [A-549] 细胞 , HL-7702 [L-02; LO2] 细胞 , BEAS-2B 细胞
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NDRG2 acts as a negative regulator of the progression of small-cell lung cancer through the modulation of the PTEN-AKT-mTOR signalling cascade (2024-03-25)
作者:Zhenchuan Ma:1.Department of Thoracic Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China. ; Yuefeng Ma:1.Department of Thoracic Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China. ; Jie Feng:1.Department of Nephrology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China. ; Zhengshui Xu:1.Department of Thoracic Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China. ; Chuantao Cheng:1.Department of Dermatology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China. ; Jie Qin:1.Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China. ; Shaomin Li:1.Department of Thoracic Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China. ; Jiantao Jiang:1.Department of Thoracic Su
期刊:NDRG2 acts as a negative regulator of the progression of small-cell lung cancer through the modulation of the PTEN-AKT-mTOR signalling cascade
DOI:10.1016/j.taap.2024.116915
影响因子 :3.8
引用产品: NCI-H446 [H446] 细胞 , NCI-H146 [H146] 细胞 , NCI-H209 [H209] 细胞 , DMS 114 细胞 , H1688 细胞
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Identification of volatile biomarkers for lung cancer from different histological sources: A comprehensive study (2024-03-31)
作者:Wei Lv:1.College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China. ; Wenmin Shi:1.College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China. ; Zhijuan Zhang:1.College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China; Institute of Mass Spectrometer and Atmospheric Environment, Jinan University, Guangzhou, 510632, China. Electronic address: zhangyan0204@126.com. ; Lihua Ru:1.College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China. ; Weisheng Feng:1.College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China. ; Hanxiao Tang:1.College of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450046, China. ; Xiangqi Wang:1.The Third Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, 450046, China. ;
期刊:Identification of volatile biomarkers for lung cancer from different histological sources: A comprehensive study
影响因子 :2.9
引用产品: A549 [A-549] 细胞 , NCI-H446 [H446] 细胞 , BEAS-2B 细胞
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Radiation-Induced Bystander Effect on the Genome of Bone Marrow Mesenchymal Stem Cells in Lung Cancer (2023-04-10)
作者:Yi-Ming Zhang:1.Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and The Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Gansu University of Chinese Medicine, Lanzhou, China. ; Li-Ying Zhang:1.Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and The Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Gansu University of Chinese Medicine, Lanzhou, China. ; 2.Gansu Institute of Cardiovascular Diseases, Lanzhou, China. ; Yang-Yang Li:1.Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and The Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Gansu University of Chinese Medicine, Lanzhou, China. ; Heng Zhou:1.Key Laboratory of Space Radiobiology of Gansu Province & Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences Inst
期刊:Radiation-Induced Bystander Effect on the Genome of Bone Marrow Mesenchymal Stem Cells in Lung Cancer
影响因子 :6.6
引用产品: A549 [A-549] 细胞 , NCI-H1299 细胞 , NCI-H446 [H446] 细胞 , LLC [LL/2; LLC1] 细胞
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Efficacy and mechanism study of cordycepin against brain metastases of small cell lung cancer based on zebrafish (2022-12-17)
作者:Shi-Ru Zhang:1.College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, China. ; Miao Pan:1.College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, China. ; Ying-Bin Gao:1.College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, China. ; Ruo-Yue Fan:1.College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, China. ; Xin-Ni Bin:1.College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, China. ; Si-Tong Qian:1.State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China. ; Cheng-Lun Tang:1.Luzhou Pinchuang Technology Co. Ltd., Nanjing Sheng Ming Yuan Health Technology Co. Ltd., Nanjing 210032, China. ; Han-Jie Ying:1.C
期刊:Efficacy and mechanism study of cordycepin against brain metastases of small cell lung cancer based on zebrafish
DOI:10.1016/j.phymed.2022.154613
影响因子 :6.7
引用产品: HITES细胞培养基 , NCI-H446 [H446] 细胞 , NCI-H69 细胞
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Circ_0089823 reinforces malignant behaviors of non-small cell lung cancer by acting as a sponge for microRNAs targeting SOX4, (2007-01-20)
作者:Jiwei Li:1.Department of Thoracic Surgery, Zhengzhou Key Laboratory for Surgical Treatment for End-stage Lung Disease, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Henan University People's Hospital, Zhengzhou, Henan, China. ; Zibo Zhu:1.Department of Thoracic Surgery, Zhengzhou Key Laboratory for Surgical Treatment for End-stage Lung Disease, Zhengzhou University People's Hospital, Henan Provincial People's Hospital, Henan University People's Hospital, Zhengzhou, Henan, China. ; Saisai Li:1.Department of Thoracic Surgery, Zhengzhou Key Laboratory for Surgical Treatment for End-stage Lung Disease, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Henan University People's Hospital, Zhengzhou, Henan, China. ; Zhijun Han:1.Department of Thoracic Surgery, Zhengzhou Key Laboratory for Surgical Treatment for End-stage Lung Disease, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Henan University People's
期刊:Circ_0089823 reinforces malignant behaviors of non-small cell lung cancer by acting as a sponge for microRNAs targeting SOX4,
影响因子 :4.8
引用产品: NCI-H1299 细胞 , HCC827 细胞 , NCI-H1437 细胞 , NCI-H446 [H446] 细胞 , A549 [A-549] 细胞 , Ham's F-12K 培养基
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