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产品概述
人滑膜成纤维细胞
Cat NO.: CP-H241
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产品名称：人滑膜成纤维细胞
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组织来源：滑膜组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人滑膜成纤维细胞分离自滑膜组织；滑膜组织是位于关节腔内面的内衬结构，各种关节内疾病均会累及滑膜。而滑膜细胞是维持关节正常功能的重要组织结构，同时在各种关节疾患中也是主要病变部位。骨关节炎(OA)以关节软骨退行性变为特征，其病理改变累及关节的各个组成部分，但绝不仅局限于软骨，还包括软骨下骨、滑膜、半月板和韧带。各组成部分的病理改变相互影响，相互作用，共同加速关节的退变。滑膜细胞是构成滑膜层的最大细胞群体，是维持关节正常功能的重要组织结构，它包埋在颗粒状无定性的基质中，基质内有分散的纤维分布。滑膜由A型(巨噬样滑膜细胞)、B型(成纤维样滑膜细胞)以及C型(树突细胞样滑膜细胞)细胞组成。
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方法简介：
普诺赛实验室分离的人滑膜成纤维细胞采用混合酶消化结合差速贴壁法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人滑膜成纤维细胞经Vimentin免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H241 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传3-5代左右 消化液 0.25%胰蛋白酶 人滑膜成纤维细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Dexmedetomidine alleviates osteoarthritis inflammation and pain through the CB2 pathway in rats (2023-04-10)
作者：Xueyan Li:1.Department of Anesthesiology, Suzhou Municipal Hospital (North District), Nanjing Medical University Affiliated Suzhou Hospital, 242, Guangji Road, Suzhou, Jiangsu, China. ; Chun Zhang:1.Department of Anesthesiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 999, Xiwang Road, Shanghai, China. ; Huaqiang Tao:1.Department of Orthopedics, The First Affiliated Hospital of Soochow University, No. 188 Shizi Street, Suzhou, Jiangsu, China. ; Xing Yang:1.Orthopedics and Sports Medicine Center, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, 242, Guangji Road, Suzhou, Jiangsu 226000, China. ; Xiaobo Qian:1.Department of Anesthesiology, Suzhou Municipal Hospital (North District), Nanjing Medical University Affiliated Suzhou Hospital, 242, Guangji Road, Suzhou, Jiangsu, China. ; Jing Zhou:1.Orthopedics and Sports Medicine Center, Suzhou Municipal Hospital, Nanjing Medical University Affiliated Suzhou Hospital, 242, Guangj
期刊：Dexmedetomidine alleviates osteoarthritis inflammation and pain through the CB2 pathway in rats
DOI：10.1016/j.intimp.2023.110134
影响因子 ：5.6
引用产品： 人滑膜成纤维细胞
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Long Non-Coding RNA AL928768.3 Promotes Rheumatoid Arthritis Fibroblast-Like Synoviocytes Proliferation, Invasion and Inflammation, While Inhibits Apoptosis Via Activating Lymphotoxin Beta Mediated NF-κB Signaling Pathway (2023-11-03)
作者：Li Sun:1.Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University; Key Laboratory of Kidney Disease Prevention and Control Technology National Key Clinical Department of Kidney Diseases, Institute of Nephrology, Zhejiang University; Zhejiang Clinical Research Center of Kidney and Urinary System Disease, Hangzhou, Zhejiang Province, China. ; Lingzhen Hu:1.Department of Rheumatology and Immunology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. ; Peirong Chen:1.Department of Rheumatology and Immunology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. ; Yongji Li:1.Department of Rheumatology and Immunology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. ; Jianxin Tu:1.Department of Rheumatology and Immunology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. ; Jianghua Chen:1.Kidney Disease Center, The First Affiliated Hospital, Co
期刊：Long Non-Coding RNA AL928768.3 Promotes Rheumatoid Arthritis Fibroblast-Like Synoviocytes Proliferation, Invasion and Inflammation, While Inhibits Apoptosis Via Activating Lymphotoxin Beta Mediated NF-κB Signaling Pathway
DOI：10.1007/s10753-023-01927-x
影响因子 ：5.1
引用产品： 人滑膜成纤维细胞
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FNDC4 reduces inflammation, proliferation, invasion and migration of rheumatoid synovial cells by inhibiting CCL2/ERK signaling (2023-12-23)
作者：Changjuan Xiao:1.Nephrology Department, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha 410000, Hunan, P.R. China. Electronic address: Xiaochange870425@163.com. ; Shuoshan Xie:1.Nephrology Department, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha 410000, Hunan, P.R. China. ; Shaxi Ouyang:1.Nephrology Department, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha 410000, Hunan, P.R. China. ;
期刊：FNDC4 reduces inflammation, proliferation, invasion and migration of rheumatoid synovial cells by inhibiting CCL2/ERK signaling
DOI：10.1016/j.tice.2023.102294
影响因子 ：2.6
引用产品： 人滑膜成纤维细胞 (类风湿关节炎) , 人滑膜成纤维细胞
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Silence of S1PR4 Represses the Activation of Fibroblast-like Synoviocytes by Regulating IL-17/STAT3 Signaling Pathway (2022-09-06)
作者：Pengyu Zhang:1.Department of Nephropathy and Rheumatism, Tongde Hospital of Zhejiang Province, Hangzhou, 310007, Zhejiang, China. ; Qiang Zhang:1.Department of Rheumatology and Immunology, The 962Nd Hospital of The PLA Joint Logistic Support Force, Harbin, Heilongjiang, 150080, China. ; Zhenxia Shao:1.Department of Gynaecology, Shaoxing Second Hospital, No. 123 Yan'an Road, Shaoxing, 312000, Zhejiang, China. shaozhenxia01@163.com. ;
期刊：Silence of S1PR4 Represses the Activation of Fibroblast-like Synoviocytes by Regulating IL-17/STAT3 Signaling Pathway
DOI：10.1007/s10753-022-01728-8
影响因子 ：4.7
引用产品： 人滑膜成纤维细胞 , 人滑膜成纤维细胞 (类风湿关节炎)
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Long noncoding RNA H19 synergizes with STAT1 to regulate SNX10 in rheumatoid arthritis (2022-11-29)
作者：Yue Sun:1.Department of Rheumatology and Immunology, Shengjing Hospital of China Medical University, Shenyang 110022, Liaoning, PR China. ; Yun Guo:1.Department of Rheumatology and Immunology, Shengjing Hospital of China Medical University, Shenyang 110022, Liaoning, PR China. ; Lihua Chang:1.Department of Rheumatology and Immunology, Shengjing Hospital of China Medical University, Shenyang 110022, Liaoning, PR China. ; Jing Zhang:1.Department of Rheumatology and Immunology, Shengjing Hospital of China Medical University, Shenyang 110022, Liaoning, PR China. Electronic address: jing_Z8103@163.com. ;
期刊：Long noncoding RNA H19 synergizes with STAT1 to regulate SNX10 in rheumatoid arthritis
DOI：10.1016/j.molimm.2022.11.018
影响因子 ：4.2
引用产品： 人滑膜成纤维细胞 , 人脐静脉内皮细胞 , 人脐静脉内皮细胞完全培养基
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