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产品概述
名称 | Ramos [RA 1] (人B淋巴细胞瘤细胞) (STR鉴定正确) |
别称 | RAMOS; Ramos 1; RA 1; RA.1; Ra #1; Ra No. 1; Ramos(RA1); Ramos-RA1; Ramos (RA 1); Ramos (RA) |
种属 | 人 |
生长特性 | 悬浮细胞 |
细胞形态 | 淋巴母细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 3×10^5-5×10^5cells/mL |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。 |
背景描述 | RAMOS细胞来源于一位3岁的白人Burkitt's淋巴瘤患者。RAMOS细胞EBV阴性,表达IL-4受体和低亲和性IgE受体(CD23);每个RAMOS细胞表面大约有1500个IL-4的结合位点。RAMOS细胞可分泌IgM,表达膜型和分泌型的免疫球蛋白。 |
年龄(性别) | 男性;3岁 |
组织来源 | B淋巴细胞 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 淋巴瘤细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~48 hours |
保藏机构 | ATCC; CRL-1596 DSMZ; ACC-603 ECACC; 85030802 ECACC; 91030710 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,11 D2S1338 20,23 D3S1358 14,15 D5S818 7,12 D7S820 11 D8S1179 13,16 D13S317 13,14 D16S539 10,13 D18S51 15 D19S433 14,15.2 D21S11 30 FGA 20,24 PentaD 10,13 PentaE 8,21 TH01 7,9.3 TPOX 8,9 vWA 15,16 D6S1043 13,15 D12S391 19,22 D2S441 11,14 -
STR鉴定图
参考文献
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Iron induces B cell pyroptosis through Tom20–Bax–caspase–gasdermin E signaling to promote inflammation post-spinal cord injury (2023-07-22)
作者:Chengjie Wu:1.Department of Traumatology and Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China. ; 2.Laboratory of New Techniques of Restoration and Reconstruction, Institute of Traumatology and Orthopedics, Nanjing University of Chinese Medicine, Nanjing, China. ; Lining Wang:1.School of Chinese Medicine, School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China. ; Sixian Chen:1.School of Chinese Medicine, School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China. ; Lei Shi:1.School of Chinese Medicine, School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China. ; Mengmin Liu:1.School of Chinese Medicine, School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China. ; Pengcheng Tu:1.Department of Traumatology and Orthopedics, Affiliated Hospital of Nanjing
期刊:Iron induces B cell pyroptosis through Tom20–Bax–caspase–gasdermin E signaling to promote inflammation post-spinal cord injury
DOI:10.1186/s12974-023-02848-0
影响因子 :9.3
引用产品: Ramos [RA 1] 细胞 , 小鼠脊髓神经元细胞
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The Amplified DNA Logic Gates Based on Aptamer–Receptor Recognition for Cell Detection and Bioimaging (2023-11-06)
作者:Yajing Wang:1.School of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China. ; Di Wu:1.School of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China. ; Xiuping Cao:1.School of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China. ; Yingshu Guo:1.School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China. ;
期刊:The Amplified DNA Logic Gates Based on Aptamer–Receptor Recognition for Cell Detection and Bioimaging
影响因子 :5.4
引用产品: Ramos [RA 1] 细胞 , Hela 细胞
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Long non-coding RNA SNHG17 may function as a competitive endogenous RNA in diffuse large B-cell lymphoma progression by sponging miR-34a-5p (2023-11-21)
作者:Shengjuan Lu:1.Department of Hematology/Oncology, Guangxi Medical University Cancer Hospital, Nanning, China. ; Lin Zeng:1.Department of Hematology/Oncology, Guangxi Medical University Cancer Hospital, Nanning, China. ; Guojun Mo:1.Department of Pharmacy, The Second Affiliated Hospital of Guangxi Medical University, Nanning, China. ; 2.Life Sciences Institute, Guangxi Medical University, Nanning, China. ; Danqing Lei:1.Life Sciences Institute, Guangxi Medical University, Nanning, China. ; Yuanhong Li:1.Department of Pharmacy, The Second Affiliated Hospital of Guangxi Medical University, Nanning, China. ; Guodi Ou:1.Pharmaceutical College, Guangxi Medical University, Nanning, China. ; Hailian Wu:1.Life Sciences Institute, Guangxi Medical University, Nanning, China. ; Jie Sun:1.Department of Hematology/Oncology, Guangxi Medical University Cancer Hospital, Nanning, China. ; Chao Rong:1.Department of Hematology/Oncology, Guangxi Medical University Cancer Hospital, Nanning, China. ;
期刊:Long non-coding RNA SNHG17 may function as a competitive endogenous RNA in diffuse large B-cell lymphoma progression by sponging miR-34a-5p
DOI:10.1371/journal.pone.0294729
影响因子 :3.7
引用产品: Daudi 细胞 , Ramos [RA 1] 细胞 , DoHH2 细胞 , Farage 细胞 , Raji 细胞
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