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产品概述
名称 | Jurkat clone A3[A3] (人T淋巴细胞白血病细胞) (STR鉴定正确) |
别称 | Jurkat clone A3 |
种属 | 人 |
生长特性 | 悬浮细胞 |
细胞形态 | 淋巴母细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 3×10^5-2×10^6cells/mL |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。 |
背景描述 | A3亚克隆株来自Jurkat细胞系,是用Fas抗体处理Jurkat细胞,随后有限稀释法获得一个细胞系。A3细胞系对Fas介导的凋亡产生自发抗性的比例较低,得到的亚克隆对Fas-介导的凋亡十分敏感。挑选对新霉素具有抗性的野生型A3细胞,并三次用移码突变诱变剂ICR-191进行处理以分离具有抗Fas抗体杀伤的隐性突变体。 |
年龄(性别) | 男 |
组织来源 | T细胞白血病,T淋巴细胞 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 白血病细胞 |
生物安全等级 | BSL-1 |
保藏机构 | ATCC; CRL-2570 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11,12 D2S1338 19,23 D3S1358 15,17 D5S818 9 D7S820 8,10 D8S1179 12,14 D13S317 8,11 D16S539 11 D18S51 13,20,21 D19S433 13,15.2 D21S11 31.2,33.2 FGA 19,22 PentaD 11 PentaE 10,12 TH01 6,9.3 TPOX 8,10 vWA 17,18 D6S1043 11 D12S391 22,24 D2S441 14 -
STR鉴定图
参考文献
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IgD/FcδR is involved in T-cell acute lymphoblastic leukemia and regulated by IgD-Fc-Ig fusion protein (2023-02-04)
作者:Yujing Wu:1.Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine, Anhui Provincial Institute of Translational Medicine, Hefei 230032, China. ; Aijun Zhang:1.Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine, Anhui Provincial Institute of Translational Medicine, Hefei 230032, China. ; Wensheng Chen:1.Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine, Anhui Provincial Institute of Translational Medicine, Hefei 230032, China. ; Qianling Xin:1.Institute of Clinical Pharmacology, Anhui M
期刊:IgD/FcδR is involved in T-cell acute lymphoblastic leukemia and regulated by IgD-Fc-Ig fusion protein
DOI:10.1016/j.phrs.2023.106686
影响因子 :9.3
引用产品: Jurkat, Clone E6-1 细胞 , MOLT-4 细胞 , EL4 细胞
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Label-free, rapid and highly accurate identification and categorization of leukemia cells via Raman spectroscopy (2023-08-22)
作者:Electronic Materials Research Laboratory, Key Laboratory of the Ministry of Education & International Center for Dielectric Research, School of Electronic Science and Engineering, Xi’an Jiaotong University, Xi’an, China Biology Program, Faculty of Science, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada
期刊:Label-free, rapid and highly accurate identification and categorization of leukemia cells via Raman spectroscopy
影响因子 :8.4
引用产品: K-562 [K562] 细胞 , U-937 细胞 , Raji 细胞 , Jurkat, Clone E6-1 细胞 , K-562 [K562]细胞专用培养基 , U-937细胞专用培养基 , Raji细胞专用培养基 , Jurkat, Clone E6-1细胞专用培养基
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BLK positively regulates TLR/IL-1R signaling by catalyzing TOLLIP phosphorylation (2023-12-11)
作者:Wei-Wei Li:1.State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China. ; 2.Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences , Wuhan, China. ; 3.Gansu Province Research Center for Basic Disciplines of Pathogen Biology , Lanzhou, China. ; Xu-Xu Fan:1.State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China. ; 2.Gansu Province Research Center for Basic Disciplines of Pathogen Biology , Lanzhou, China. ; Zhi-Sheng Xu:1.Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences , Wuhan, China. ; Zi-Xiang Zhu:
期刊:BLK positively regulates TLR/IL-1R signaling by catalyzing TOLLIP phosphorylation
影响因子 :7.8
引用产品: U-87 MG 细胞 , Jurkat, Clone E6-1 细胞 , Raji 细胞 , A20 细胞
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IL-17A promotes tumorigenesis and upregulates PD-L1 expression in non-small cell lung cancer (2023-11-17)
作者:Hua Liao:1.Chronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China. ; 2.Department of Respiratory and Critical Care Medicine, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China. ; Xiaodan Chang:1.Chronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China. ; 2.School of Nursing, Southern Medical University, Guangzhou, Guangdong, China. ; Lin Gao:1.Chronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China. ; Cuiping Ye:1.Chronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China. ; Yujie Qiao:1.Chronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Med
期刊:IL-17A promotes tumorigenesis and upregulates PD-L1 expression in non-small cell lung cancer
DOI:10.1186/s12967-023-04365-3
影响因子 :7.4
引用产品: Jurkat, Clone E6-1 细胞
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Yi-Qi-Jian-Pi formula ameliorates immune function in acute-on-chronic liver failure by upregulating autophagy and mitochondrial biogenesis in CD8+ T lymphocytes (2023-02-17)
作者:Li Tang:1.Department of Integrated TCM and Western Medicine, Nanjing Hospital Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210003, China; Department of Gastroenterology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210022, China. ; Xi Wang:1.Department of Integrated TCM and Western Medicine, Nanjing Hospital Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210003, China. ; Rong Zhao:1.Department of Integrated TCM and Western Medicine, Nanjing Hospital Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210003, China. ; Xiaomei Chen:1.Department of Integrated TCM and Western Medicine, Nanjing Hospital Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210003, China. ; Feixia Wang:1.Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Material Medical, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China. ; Siwei Xia:1.Jiangs
期刊:Yi-Qi-Jian-Pi formula ameliorates immune function in acute-on-chronic liver failure by upregulating autophagy and mitochondrial biogenesis in CD8+ T lymphocytes
影响因子 :5.4
引用产品: RPMI-1640 培养基 , Jurkat, Clone E6-1 细胞
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Down-regulation of Musashi-2 exerts antileukemic effects on acute lymphoblastic leukemia cells and increases sensitivity to dexamethasone (2023-09-26)
作者:Duobing Zou:1.Laboratory of Stem Cell Transplantation, Ningbo First Hospital, Ningbo, Zhejiang, 315000, People's Republic of China. ; Mei Lv:1.Department of Hematology, Ningbo Chinese Medical Hospital, Ningbo, Zhejiang, 315000, People's Republic of China. ; Ying Chen:1.Laboratory of Stem Cell Transplantation, Ningbo First Hospital, Ningbo, Zhejiang, 315000, People's Republic of China. ; Tingting Niu:1.Laboratory of Stem Cell Transplantation, Ningbo First Hospital, Ningbo, Zhejiang, 315000, People's Republic of China. ; Chao Ma:1.Laboratory of Stem Cell Transplantation, Ningbo First Hospital, Ningbo, Zhejiang, 315000, People's Republic of China. ; Cong Shi:1.Laboratory of Stem Cell Transplantation, Ningbo First Hospital, Ningbo, Zhejiang, 315000, People's Republic of China. ; Zhenya Huang:1.Laboratory of Stem Cell Transplantation, Ningbo First Hospital, Ningbo, Zhejiang, 315000, People's Republic of China. ; Ying Wu:1.Laboratory of Stem Cell Transplantation, Ningbo First Hospital
期刊:Down-regulation of Musashi-2 exerts antileukemic effects on acute lymphoblastic leukemia cells and increases sensitivity to dexamethasone
DOI:10.1007/s00277-023-05468-z
影响因子 :3.5
引用产品: Jurkat, Clone E6-1 细胞
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Hyposterolactone A, a 3α-Hydroxy Steroidal Lactone from the Deep-Sea-Derived Fungus Hypocrea sp. ZEN14 (2023-06-02)
作者:Lin Xu:1.Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, 184 Daxue Road, Xiamen 361005, China. ; Hua-Bin Ma:1.Central Laboratory, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China. ; Taizong Wu:1.Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, 184 Daxue Road, Xiamen 361005, China. ; 2.Institute for Molecular Bioscience, University of Queensland, Brisbane, 4072, Australia. ; Li-Fang Liu:1.Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, 184 Daxue Road, Xiamen 361005, China. ; Ming-Min Xie:1.Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, 184 Daxue Road, Xiamen 361005, China. ; Man-Yi Hu:1.Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, 184 Daxu
期刊:Hyposterolactone A, a 3α-Hydroxy Steroidal Lactone from the Deep-Sea-Derived Fungus Hypocrea sp. ZEN14
影响因子 :2.9
引用产品: HuH-7 细胞 , Hep G2 细胞 , HL-60 细胞 , Jurkat, Clone E6-1 细胞
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Human EVI2B acts as a Janus-faced oncogene/antioncogene by differently affecting as per cancer type neoplastic cells growth and immune infiltration (2023-03-23)
作者:Fangyingnan Zhang: 1.School of Biomedical Engineering, Sun Yat-sen University, Guangzhou, Guangdong, People’s Republic of China; Department of Burn and Plastic Surgery, Shenzhen Institute of Translational Medicine, Shenzhen, Guangdong, People’s Republic of China; Xinning Wang: 1.School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong, People’s Republic of China; Xiaoqi Zhang: 1.The Center for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou, Guangdong, People’s Republic of China; Saquib Waheed: 1.Department of Burn and Plastic Surgery, Shenzhen Institute of Translational Medicine, Shenzhen, Guangdong, People’s Republic of China; Rong Zhong: 1.Department of Burn and Plastic Surgery, Shenzhen Institute of Translational Medicine, Shenzhen, Guangdong, People’s Republic of China; Ubaldo Armato: 1.Department of Burn and Plastic Surgery, Shenzhen Institute of Translational Medicine, Shenzhen, Guangdong, People’s Republic of China; Section of Human Hi
期刊:Human EVI2B acts as a Janus-faced oncogene/antioncogene by differently affecting as per cancer type neoplastic cells growth and immune infiltration
DOI:10.1515/oncologie-2022-1002
影响因子 :0.9
引用产品: U-937 细胞 , Jurkat, Clone E6-1 细胞 , A549 [A-549] 细胞 , A-375 细胞 , Hep G2 细胞 , U-87 MG 细胞
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1,25(OH)2D3 Promotes Macrophage Efferocytosis Partly by Upregulating ASAP2 Transcription via the VDR-Bound Enhancer Region and ASAP2 May Affect Antiviral Immunity (2022-11-21)
作者:Hui Shi:1.Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China. ; Jiangling Duan:1.Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China. ; Jiayu Wang:1.Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China. ; Haohao Li:1.Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China. ; Zhiheng Wu:1.Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China. ; Shuaideng Wang:1.Department of Human Anatomy, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China. ; Xueyan Wu:1.Department of Human Anatomy, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China. ; Ming Lu:1.Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China. ;
期刊:1,25(OH)2D3 Promotes Macrophage Efferocytosis Partly by Upregulating ASAP2 Transcription via the VDR-Bound Enhancer Region and ASAP2 May Affect Antiviral Immunity
影响因子 :6.7
引用产品: THP-1 细胞 , J774A.1 细胞 , Jurkat, Clone E6-1 细胞
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