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产品概述
DMEM/F12培养基是在DMEM培养基的基础上,添加F12培养基中更为丰富的营养成分,含有多种微量元素,广泛应用于多种哺乳类细胞的培养。同时,DMEM/F12培养基常作为开发无血清培养基的基础,也适用于低血清含量下哺乳动物细胞的培养以及克隆密度培养。不含葡萄糖的DMEM/F12培养基可以根据研究需要,随意调节葡萄糖的浓度,方便快捷。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分,但不含蛋白质、脂类或任何生长因子,故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
L-谷氨酰胺 | 2.5 mM |
NaHCO3 | 2438 mg/L |
D-葡萄糖 | 无 |
丙酮酸钠 | 0.5 mM |
HEPES缓冲剂 | 无 |
酚红指示剂 | 8.1 mg/L |
储存条件 | 2-8℃,避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌,使用时应注意无菌操作,避免污染;
2、为保持本产品的最佳使用效果,请勿进行冻融处理;
3、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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Canagliflozin regulates metabolic reprogramming in diabetic kidney disease by inducing fasting-like and aestivation-like metabolic patterns (2024-01-18)
作者:Mingwei Shao:1.Department of Endocrinology and Metabolism, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China. ; Duo Chen:1.Institute of Clinical Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China. ; Qingzhu Wang:1.Department of Endocrinology and Metabolism, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China. ; Feng Guo:1.Department of Endocrinology and Metabolism, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China. ; Fangyi Wei:1.Institute of Clinical Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China. ; Wei Zhang:1.Institute of Clinical Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China. ; Tian Gan:1.Institute of Clinical Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China. ; Yuanyuan Luo:1.Institute of Clinical Medicine, The First Affiliated Hos
期刊:Canagliflozin regulates metabolic reprogramming in diabetic kidney disease by inducing fasting-like and aestivation-like metabolic patterns
DOI:10.1007/s00125-023-06078-0
影响因子 :8.2
引用产品: DMEM/F12无糖 培养基
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The Effectiveness of Four Nicotinamide Adenine Dinucleotide (NAD+) Precursors in Alleviating the High-Glucose-Induced Damage to Hepatocytes in Megalobrama amblycephala: Evidence in NAD+ Homeostasis, Sirt1/3 Activation, Redox Defense, Inflammatory Response, Apoptosis, and Glucose Metabolism (2024-03-22)
作者:Yanzou Dong:1.Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, China. ; Xi Wang:1.Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, China. ; Luyao Wei:1.Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, China. ; Zishang Liu:1.Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, China. ; Xiaoyu Chu:1.Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 We
期刊:The Effectiveness of Four Nicotinamide Adenine Dinucleotide (NAD+) Precursors in Alleviating the High-Glucose-Induced Damage to Hepatocytes in Megalobrama amblycephala: Evidence in NAD+ Homeostasis, Sirt1/3 Activation, Redox Defense, Inflammatory Response, Apoptosis, and Glucose Metabolism
影响因子 :7.0
引用产品: DMEM/F12无糖 培养基
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Kaempferol regulates apoptosis and migration of neural stem cells to attenuate cerebral infarction by O‐GlcNAcylation of β-catenin (2024-03-09)
作者:Song Zhang:1.Department of Neurology, The Second Hospital of Hebei Medical University, No. 215, Heping West Road, Shijiazhuang, Hebei 050000, China. ; Honglei Jiao:1.Department of Neurology, The Second Hospital of Hebei Medical University, No. 215, Heping West Road, Shijiazhuang, Hebei 050000, China. ;
期刊:Kaempferol regulates apoptosis and migration of neural stem cells to attenuate cerebral infarction by O‐GlcNAcylation of β-catenin
影响因子 :2.2
引用产品: DMEM/F12无糖 培养基
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Metabolic Patterns of High-Invasive and Low-Invasive Oral Squamous Cell Carcinoma Cells Using Quantitative Metabolomics and 13C-Glucose Tracing (2023-12-18)
作者:Wenrong Jiang:1.Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China. ; 2.Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China. ; 3.Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China. ; Ting Zhang:1.Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China. ; 2.Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China. ; 3.Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China. ; Hua Zhang:1.Ministry of Education of China International Collaborative Joint Laboratory of Reproduction and Development, Chongqing Medical University, Chongqing 400016, China. ; 2.State Key Laboratory of Maternal and Fetal Medicine of Chongqing Municipality, Chongqing Medical University, Chongqing 400016, China. ; 3.Institute of Life Sciences, Chongqing Medical University, C
期刊:Metabolic Patterns of High-Invasive and Low-Invasive Oral Squamous Cell Carcinoma Cells Using Quantitative Metabolomics and 13C-Glucose Tracing
影响因子 :5.5
引用产品: DMEM/F12无糖 培养基
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Effects of borneol combined with astragaloside IV and Panax notoginseng saponins regulation of microglia polarization to promote neurogenesis after cerebral ischaemia (2023-04-26)
作者:Huang Ding:1.Hunan University of Chinese Medicine, Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Changsha, China. ; Xiao-Ping Huang:1.Hunan University of Chinese Medicine, Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Changsha, China. ; Xiao-Dan Liu:1.Hunan University of Chinese Medicine, Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Changsha, China. ; Yan-Ling Li:1.Hunan University of Chinese Medicine, Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Changsha, China. ; San Tang:1.Hunan University of Chinese Medicine, Key Laboratory of Hunan Province for Integrated Traditional Chinese and Wes
期刊:Effects of borneol combined with astragaloside IV and Panax notoginseng saponins regulation of microglia polarization to promote neurogenesis after cerebral ischaemia
影响因子 :3.3
引用产品: DMEM/F12 培养基 , DMEM/F12无糖 培养基 , 特级胎牛血清
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KIM-1 augments hypoxia-induced tubulointerstitial inflammation through uptake of small extracellular vesicles by tubular epithelial cells (2022-08-18)
作者:Jun Chen:1.Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, 87 Ding Jia Qiao Road, Nanjing 210009, China. ; Tao-Tao Tang:1.Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, 87 Ding Jia Qiao Road, Nanjing 210009, China. ; Jing-Yuan Cao:1.Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, 87 Ding Jia Qiao Road, Nanjing 210009, China. ; Zuo-Lin Li:1.Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, 87 Ding Jia Qiao Road, Nanjing 210009, China. ; Xin Zhong:1.Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, 87 Ding Jia Qiao Road, Nanjing 210009, China. ; Yi Wen:1.Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, 87 Ding Jia Qiao Road, Nanjing 210009, China. ; An-Ran Shen:1.Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, 87 Ding Jia Qiao Road
期刊:KIM-1 augments hypoxia-induced tubulointerstitial inflammation through uptake of small extracellular vesicles by tubular epithelial cells
DOI:10.1016/j.ymthe.2022.08.013
影响因子 :12.9
引用产品: DMEM/F12无糖 培养基
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