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产品概述
人胰腺星状细胞
Cat NO.: CP-H024
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产品名称：人胰腺星状细胞
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英文简称：PSCs
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组织来源：胰腺组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人胰腺星状细胞分离自胰腺组织；胰腺分为外分泌腺和内分泌腺两部分。外分泌腺由腺泡和腺管组成，腺泡分泌胰液，腺管是胰液排出的通道。胰液中含有碳酸氢钠、胰蛋白酶原、脂肪酶、淀粉酶等。胰液通过胰腺管排入十二指肠，有消化蛋白质、脂肪和糖的作用。内分泌腺由大小不同的细胞团──胰岛所组成，胰岛主要由4种细胞组成：α细胞、β细胞、γ细胞及PP细胞。α细胞分泌胰高血糖素，升高血糖；β细胞分泌胰岛素，降低血糖；γ细胞分泌生长抑素，以旁分泌的方式抑制α、β细胞的分泌；PP细胞分泌胰多肽，抑制胃肠运动、胰液分泌和胆囊收缩。纤维化是慢性胰腺炎的典型病理特征，活化的胰腺星状细胞(PSC)是胰腺纤维化的主要效应细胞，PSC分离和成功培养是体外研究胰腺纤维化的重要前提。未活性化的PSC胞浆中富含维A脂滴，并表达Desmin蛋白，活化后的PSC则表达α-平滑肌肌动蛋白(alpha-SMA)。PSC具有静息态与激活态两种，并分别具有特异的标志物。静息状态PSC胞质内富含的维生素A脂滴可以被油红O染成红色，阳性表达Desmin。激活状态PSC阳性表达alpha-SMA。油红O染色发现，原代分离的细胞培养6d，胞浆中仍可见明显的脂滴，传代后，橙红色的脂滴颗粒显著减少甚至消失。免疫细胞化学染色显示，细胞接种24h仍然表达Desmin，48h后Desmin基本不再表达。培养48h后绝大多数细胞开始表达alpha-SMA，随着培养时间的增长和传代次数的增加，细胞表达alpha-SMA，而不再表达Desmin，说明细胞活化。提示PSC接种24h后即启动了激活过程，至培养第6d大多数细胞激活，传代后细胞处于高度激活状态。原代胰腺星状细胞可作为慢性胰腺炎新药的细胞筛选模型，目前研究发现：胰腺受损时，在各种刺激因子作用下使胰腺星状细胞活化，导致细胞形态、功能发生变化，促使基质增生、胶原蛋白的大量生成及不规则沉积。
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方法简介：
普诺赛实验室分离的人胰腺星状细胞采用胶原酶制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人胰腺星状细胞经α-SMA或Desmin免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H024 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 人胰腺星状细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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ITGB6 promotes pancreatic fibrosis and aggravates the malignant process of pancreatic cancer via JAK2/STAT3 signaling pathway (2024-02-28)
作者：Yu Zhang:1.Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, People's Republic of China. ; 2.Department of Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, People's Republic of China. ; 3.Department of Emergency Surgery, The Lu'an Hospital Affiliated to Anhui Medical University, The Lu'an People's Hospital, Lu'an, Anhui, 237005, People's Republic of China. ; Zhiyuan Chen:1.Department of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of Wannan Medical College, No.2 Zheshan West Road, Jinghu District, Wuhu, Anhui, 241001, People's Republic of China. ; Zhengchao Shen:1.Department of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of Wannan Medical College, No.2 Zheshan West Road, Jinghu District, Wuhu, Anhui, 241001, People's Republic of China. ; Daohai Qian:1.Department of Hepatopancreatobiliary Surgery, The First Affiliated
期刊：ITGB6 promotes pancreatic fibrosis and aggravates the malignant process of pancreatic cancer via JAK2/STAT3 signaling pathway
DOI：10.1007/s00210-024-03003-z
影响因子 ：3.6
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Pancreatic stellate cell-derived exosomal tRF-19-PNR8YPJZ promotes proliferation and mobility of pancreatic cancer through AXIN2 (2023-07-24)
作者：Wenpeng Cao:1.Department of Anatomy, School of Basic Medicine, Guizhou Medical University, Guiyang, China. ; Shisi Dai:1.Department of Anatomy, School of Basic Medicine, Guizhou Medical University, Guiyang, China. ; 2.Department of Anatomy, School of Basic Medicine, Guizhou Nursing Vocational college, Guiyang, China. ; Wanyuan Ruan:1.School of Clinical Medicine, Guizhou Medical University, Guiyang, China. ; Tingting Long:1.Department of Anatomy, School of Basic Medicine, Guizhou Medical University, Guiyang, China. ; Zhirui Zeng:1.Department of Physiology, School of Basic Medicine, Guizhou Medical University, Guiyang, China. ; Shan Lei:1.Department of Physiology, School of Basic Medicine, Guizhou Medical University, Guiyang, China. ;
期刊：Pancreatic stellate cell-derived exosomal tRF-19-PNR8YPJZ promotes proliferation and mobility of pancreatic cancer through AXIN2
影响因子 ：5.3
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TGF-β1 induced proliferation, migration, and ECM accumulation through the SNHG11/miR-34b/LIF pathway in human pancreatic stellate cells (2007-01-20)
作者：Desheng Wu:1.Department of Emergency Medicine, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China. ; Jin Guo:1.Department of Emergency Medicine, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China. ; Benquan Qi:1.Department of Emergency Medicine, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China. ; Heng Xiao:1.Department of Emergency Medicine, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China. ;
期刊：TGF-β1 induced proliferation, migration, and ECM accumulation through the SNHG11/miR-34b/LIF pathway in human pancreatic stellate cells
影响因子 ：2.9
引用产品： 人胰腺星状细胞
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