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人肝内胆管上皮细胞
Human Intrahepatic Bile Duct Epithelial Cells
货号：CP-H042
规格：5×10⁵Cells/T25培养瓶
价格： ￥8380
培养基货号： CM-H042
产品概述
人肝内胆管上皮细胞
Cat NO.: CP-H042
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产品名称：人肝内胆管上皮细胞
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组织来源：肝脏组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人肝内胆管上皮细胞分离自肝脏组织；肝脏是身体内以代谢功能为主的一个器官，并在身体里面起着去氧化、储存肝糖、分泌性蛋白质的合成等作用；肝脏也制造消化系统中之胆汁。肝脏是机体内脏里最大的器官，位于机体中的腹部位置，在右侧横隔膜之下，位于胆囊之前端且于右边肾脏的前方，胃的上方。肝脏是机体消化系统中最大的消化腺，为一红棕色的V字形器官。肝脏是尿素合成的主要器官，又是新陈代谢的重要器官。肝脏在机体位置和形态结构：肝脏位于右上腹，隐藏在右侧膈下和肋骨深面，大部分肝为肋弓所复盖，仅在腹上区、右肋弓间露出并直接接触腹前壁，肝上面则与膈及腹前壁相接。通过控制激素调控的分泌和吸收，在保持、调整和扩大胆小管的结构中发挥重要作用，肝内胆管上皮细胞在先天性和获得性免疫应答方面起着积极作用。肝内胆管上皮细胞约占肝细胞总数的5%，其在肝内胆道系统内形成复杂的网状管形结构。肝内胆管上皮细胞具有复杂的生理代谢功能，参与肝脏的代谢、排泌、免疫等生理过程，在肝脏疾病的发生发展过程中起一定的作用。研究表明，常见的肝内胆管病变例如原发性硬化性胆管炎、胆管癌等疾病，都是以肝内胆管上皮细胞为病变靶位，进而引起肝内胆管上皮损伤。因此，了解正常肝内胆管上皮细胞的生物学特征和病变肝内胆管上皮细胞的病理特征对研究肝内胆管病变的发病机制、诊断和治疗具有重要意义。
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方法简介：
普诺赛实验室分离的人肝内胆管上皮细胞采用胶原酶灌注消化法后，再用胶原酶-DNA酶联合消化，接种至预先包被鼠尾胶原的培养瓶中，通过上皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人肝内胆管上皮细胞经Cytokeratin-19免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 鼠尾胶原Ⅰ(2-5μg/cm2) 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H042 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 上皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 人肝内胆管上皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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A Novel Trojan Horse Nanotherapy Strategy Targeting the cPKM-STMN1/TGFB1 Axis for Effective Treatment of Intrahepatic Cholangiocarcinoma (2023-10-03)
作者：Zhi-Wen Chen:1.Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, China. ; Feng-Ping Kang:1.Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, China. ; Cheng-Ke Xie:1.Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, China. ; Cheng-Yu Liao:1.Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, China. ; Ge Li:1.Department of Hepatobiliary Surgery and Fujian Institute of Hepatobiliary Surgery, Fujian Medical University Union Hospital, Fuzhou, 350001, China. ; Yong-Ding Wu:1.Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, China. ; Hong-Yi Lin:1.Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, China. ; Shun-Cang Zhu:1.Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, China. ; Jian-Fei Hu:1.Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, China. ; Cai-Fen
期刊：A Novel Trojan Horse Nanotherapy Strategy Targeting the cPKM-STMN1/TGFB1 Axis for Effective Treatment of Intrahepatic Cholangiocarcinoma
影响因子 ：15.1
引用产品： 人肝内胆管上皮细胞 , 小鼠肝枯否细胞 , 人肝内胆管上皮细胞完全培养基 , 小鼠肝枯否细胞完全培养基
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The protective effect of forsythiaside A on 3,5-diethoxycarbonyl-1,4-dihydrocollidine-induced cholestatic liver injury in mice: Based on targeted metabolomics and molecular biology technology (2023-07-29)
作者：Ke Fu:1.State Key Laboratory of Southwestern Chinese Medicine Resources, Key Laboratory of Standardization for Chinese Herbal Medicine, Ministry of Education, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. ; Shu Dai:1.State Key Laboratory of Southwestern Chinese Medicine Resources, Key Laboratory of Standardization for Chinese Herbal Medicine, Ministry of Education, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. ; Yanzhi Li:1.State Key Laboratory of Southwestern Chinese Medicine Resources, Key Laboratory of Standardization for Chinese Herbal Medicine, Ministry of Education, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. ; Cheng Ma:1.State Key Laboratory of Southwestern Chinese Medicine Resources, Key Laboratory of Standardization for Chinese Herbal Medicine, Ministry of Education, School of Pharmacy, Chengdu University of Traditional Chinese
期刊：The protective effect of forsythiaside A on 3,5-diethoxycarbonyl-1,4-dihydrocollidine-induced cholestatic liver injury in mice: Based on targeted metabolomics and molecular biology technology
DOI：10.1016/j.bbadis.2023.166822
影响因子 ：6.2
引用产品： HL-7702 [L-02; LO2] 细胞 , 人肝内胆管上皮细胞
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MUC1 induces the accumulation of Foxp3+ Treg cells in the tumor microenvironment to promote the growth and metastasis of cholangiocarcinoma through the EGFR/PI3K/Akt signaling pathway (2023-04-03)
作者：Guofeng Zhang:1.Department of Hepatobiliary and Pancreatic Surgery, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, PR China. ; Ge Zheng:1.Department of Hepatobiliary and Pancreatic Surgery, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, PR China. ; Haipeng Zhang:1.Department of Medicine, The Hospital of Northeast Normal University, Changchun 130024, Jilin Province, PR China. ; Ling Qiu:1.Department of Radiation Oncology, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, PR China. Electronic address: ql1433@jlu.edu.cn. ;
期刊：MUC1 induces the accumulation of Foxp3+ Treg cells in the tumor microenvironment to promote the growth and metastasis of cholangiocarcinoma through the EGFR/PI3K/Akt signaling pathway
DOI：10.1016/j.intimp.2023.110091
影响因子 ：5.6
引用产品： 人肝内胆管上皮细胞
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Guanine nucleotide exchange factor T exerts the cancer-promoting function in cholangiocarcinoma by enhancing the Wnt-GSK-3β-β-catenin cascade via regulation of Rac1/Cdc42 (2023-03-26)
作者：Xifang Wang:1.Department of Medical Oncology, Shaanxi Provincial People's Hospital, Xi'an 710068, China. ; Xiaomin Zhang:1.Department of Orthopaedics, Xi'an International Medical Center Hospital, Xi'an 710100, China. ; Jingying Sun:1.Shaanxi Provincial Key Laboratory of Infectious and Immunological Diseases, Shaanxi Provincial People's Hospital, Xi'an 710068, China. ; Yang Sun:1.Data Center, Shaanxi Provincial People's Hospital, s 710068, China. ; Yuan Zhang:1.Department of Medical Oncology, Shaanxi Provincial People's Hospital, Xi'an 710068, China. ; Li He:1.Department of Medical Oncology, Shaanxi Provincial People's Hospital, Xi'an 710068, China. ; Ping Wang:1.Department of Pathology, Shaanxi Provincial People's Hospital, Xi'an 710068, China. ; Feng Li:1.Department of Pathology and Medical Research Center, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China. Electronic address: li_feng7855@163.com. ; Chao Sun:1.Department of Medical Oncology, Shaanxi
期刊：Guanine nucleotide exchange factor T exerts the cancer-promoting function in cholangiocarcinoma by enhancing the Wnt-GSK-3β-β-catenin cascade via regulation of Rac1/Cdc42
DOI：10.1016/j.taap.2023.116492
影响因子 ：3.8
引用产品： 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100× , HCCC-9810 细胞 , 人肝内胆管上皮细胞 , 人肝内胆管上皮细胞完全培养基
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Hsa_circ_0019054 up-regulates HIF1A through sequestering miR-340–5p to promote the tumorigenesis of intrahepatic cholangiocarcinoma (2022-12-06)
作者：Jintian Tang:1.Department of Hepatopancreatobiliary, Cancer Hospital Affiliated to Xinjiang Medical University, Urumqi, China. ; Runjuan Tang:1.Rehabilitation Department, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi, China. ; Peng Gu:1.Interventional Department, Cancer Hospital Affiliated to Xinjiang Medical University, Urumqi, China. ; Jing Han:1.Office of Drug Clinical Trial Institutions, Cancer Hospital Affiliated to Xinjiang Medical University, Urumqi, China. ; Wukui Huang:1.Interventional Department, Cancer Hospital Affiliated to Xinjiang Medical University, Urumqi, China. ; Feng Xue:1.Department of Hepatopancreatobiliary, Cancer Hospital Affiliated to Xinjiang Medical University, Urumqi, China. ;
期刊：Hsa_circ_0019054 up-regulates HIF1A through sequestering miR-340–5p to promote the tumorigenesis of intrahepatic cholangiocarcinoma
影响因子 ：3.2
引用产品： 人肝内胆管上皮细胞 , RBE 细胞 , HuCC-T1 细胞
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Circ_0000284 facilitates the growth, metastasis and glycolysis of intrahepatic cholangiocarcinoma through miR-152-3p-mediated PDK1 expression. (2022-11-04)
作者：Jian Sun:1.Department II of General Surgery, Hanchuan People's Hospital, Hanchuan, Hubei, China. ; Menglong Feng:1.Department II of General Surgery, Hanchuan People's Hospital, Hanchuan, Hubei, China. ; Huilian Zou:1.Department of Gynaecology and Obstetrics, Hanchuan People's Hospital, Hanchuan, Hubei, China. ; Yanping Mao:1.Department II of General Surgery, Hanchuan People's Hospital, Hanchuan, Hubei, China. ; Wei Yu:1.Department II of General Surgery, Hanchuan People's Hospital, Hanchuan, Hubei, China. yuwei19811110@163.com. ;
期刊：Circ_0000284 facilitates the growth, metastasis and glycolysis of intrahepatic cholangiocarcinoma through miR-152-3p-mediated PDK1 expression.
影响因子 ：2.1
引用产品： RBE 细胞 , HCCC-9810 细胞 , 人肝内胆管上皮细胞
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RANK/RANKL Acts as a Protective Factor by Targeting Cholangiocytes in Primary Biliary Cholangitis (2008-09-20)
作者：Yan-Li Hao:1.Center for Liver Diseases, Nantong Third People's Hospital, Nantong University, 60 Mid-Youth Road, Nantong, 226006, Jiangsu, China. ; Zhao-Lian Bian:1.Center for Liver Diseases, Nantong Third People's Hospital, Nantong University, 60 Mid-Youth Road, Nantong, 226006, Jiangsu, China. ; 2.Division of Gastroenterology and Hepatology, Shanghai Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200001, China. ; Lin-Ling Ju:1.Center for Liver Diseases, Nantong Third People's Hospital, Nantong University, 60 Mid-Youth Road, Nantong, 226006, Jiangsu, China. ; Yuan Liu:1.Department of Gastroenterology, Shanghai Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200233, China. ; Gang Qin:1.Center for Liver Diseases, Nantong Third People's Hospital, Nantong University, 60 Mid-Youth Road, Nantong, 226006, Jiangsu, China. tonygqin@ntu.edu.cn. ;
期刊：RANK/RANKL Acts as a Protective Factor by Targeting Cholangiocytes in Primary Biliary Cholangitis
DOI：10.1007/s10620-019-05758-5
影响因子 ：3.1
引用产品： 人肝内胆管上皮细胞
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