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大鼠皮层神经元细胞
Cat NO.: CP-R105
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产品名称：大鼠皮层神经元细胞
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组织来源：脑组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
大鼠皮层神经元细胞分离自脑皮层组织；皮层神经元细胞是构成中枢神经系统结构和功能的基本单位。神经元是具有长突触(轴突)的细胞，它由细胞体和细胞突起构成。在长的轴突上套有一层鞘，组成神经纤维，它的末端的细小分支叫做神经末梢。细胞体位于脑、脊髓和神经节中，细胞突起可延伸至全身各器官和组织中。细胞体是细胞含核的部分，其形状大小有很大差别，直径约4-120微米。核大而圆，位于细胞中央，染色质少，核仁明显。细胞质内有斑块状的核外染色质，还有许多神经元纤维。细胞突起是由细胞体延伸出来的细长部分，又可分为树突和轴突。每个神经元可以有一或多个树突，可以接受刺激并将兴奋传入细胞体。每个神经元只有一个轴突，可以把兴奋从胞体传送到另一个神经元或其他组织，如肌肉或腺体。皮质神经元是大脑皮质的主要组成细胞之一，是大脑进行功能活动调节的基本单位，参与动物多种中枢神经系统疾病的病理过程。通过形态学观察显示神经元从贴壁、伸出突起开始；突起逐渐增多，而后突起进一步增多并逐渐成网，同时细胞胞体增大，周边光晕明显。10-12d细胞最为丰满，随后神经元开始裂解，突起逐渐减少，细胞已退化变性，轮廓模糊，光晕消失，细胞变形。
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方法简介：
普诺赛实验室分离的大鼠皮层神经元细胞采用胰蛋白酶消化法、神经元专用培养基培养筛选结合化学试剂抑制法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的大鼠皮层神经元细胞经β-Tubulin-Ⅲ免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 PLL(0.1mg/ml) 培养基 含B-27 Supplement、Penicillin、Streptomycin等 产品货号 CM-R105 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 神经元细胞样 传代特性 属于终末分化细胞；属于不增殖细胞群 消化液 0.125%胰蛋白酶 大鼠皮层神经元细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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3D bio-printed living nerve-like fibers refine the ecological niche for long-distance spinal cord injury regeneration (2023-02-02)
作者：Jia Yang:1.State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Beijing, 100084, China. ; Kaiyuan Yang:1.Department of Neurosurgery, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, 102218, China. ; Weitao Man:1.Department of Neurosurgery, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, 102218, China. ; Jingchuan Zheng:1.State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Beijing, 100084, China. ; Zheng Cao:1.State Key Laboratory of New Ceramics and Fine Processing, Key Laboratory of Advanced Materials, School of Materials Science and Engineering, Tsinghua University, Beijing, 100084, China. ; Chun-Yi Yang:1.State Key Laboratory of New Ceramics and Fine Processing, Key Lab
期刊：3D bio-printed living nerve-like fibers refine the ecological niche for long-distance spinal cord injury regeneration
DOI：10.1016/j.bioactmat.2023.01.023
影响因子 ：18.9
引用产品： 大鼠皮层神经元细胞
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Pregabalin inhibits purinergic P2Y2 receptor and TRPV4 to suppress astrocyte activation and to relieve neuropathic pain (2023-11-02)
作者：Shasha Song:1.Rehabilitation Center, Qilu Hospital of Shandong University, Jinan, Shandong, China. ; Qianwen Wang:1.The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital of Shandong University, Jinan, Shandong, China. ; Yujuan Qu:1.Rehabilitation Center, Qilu Hospital of Shandong University, Jinan, Shandong, China. ; Wenshuang Gao:1.Rehabilitation Center, Qilu Hospital of Shandong University, Jinan, Shandong, China. ; Danyang Li:1.Rehabilitation Center, Qilu Hospital of Shandong University, Jinan, Shandong, China. ; Xiaoqian Xu:1.Rehabilitation Center, Qilu Hospital of Shandong University, Jinan, Shandong, China. ; Shouwei Yue:1.Rehabilitation Center, Qilu Hospital of Shandong University, Jinan, Shandong, China. Electronic ad
期刊：Pregabalin inhibits purinergic P2Y2 receptor and TRPV4 to suppress astrocyte activation and to relieve neuropathic pain
DOI：10.1016/j.ejphar.2023.176140
影响因子 ：5.0
引用产品： 大鼠皮层神经元细胞 , 大鼠皮层神经元细胞完全培养基
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Neuroglobin protects against cerebral ischemia/reperfusion injury in rats by suppressing mitochondrial dysfunction and endoplasmic reticulum stress-mediated neuronal apoptosis through synaptotagmin-1 (2023-05-17)
作者：Lihong Zhang:1.Department of Neurointervention and Neurocritical Care, Dalian Central Hospital Affiliated to Dalian University of Technology, Dalian, China. ; Di Li:1.Department of Neurointervention and Neurocritical Care, Dalian Central Hospital Affiliated to Dalian University of Technology, Dalian, China. ; Lin Yin:1.Department of Neurology, The Second Affiliated Hospital of Dalian Medical University, Dalian, China. ; Ce Zhang:1.Director's Office, The Second Affiliated Hospital of Dalian Medical University, Dalian, China. ; Hong Qu:1.Bidding and Procurement Office, The Second Affiliated Hospital of Dalian Medical University, Dalian, China. ; Jianping Xu:1.Department of Cardiology, The First Affiliated Hospital of Soochow University, Suzhouy, China. ;
期刊：Neuroglobin protects against cerebral ischemia/reperfusion injury in rats by suppressing mitochondrial dysfunction and endoplasmic reticulum stress-mediated neuronal apoptosis through synaptotagmin-1
影响因子 ：4.5
引用产品： 大鼠皮层神经元细胞
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Exosome-shuttled MYCBPAP from bone marrow mesenchymal stem cells regulates synaptic remodeling and ameliorates ischemic stroke in rats (2023-07-07)
作者：Qiuyue Yan:1.Department of Neurology, Cangzhou Central Hospital, Cangzhou 061001 Hebei, PR China. Electronic address: Yanqiuyue941@163.com. ; Yong Yin:1.Department of Neurology, Cangzhou Central Hospital, Cangzhou 061001 Hebei, PR China. ; Xuechun Li:1.Department of Neurology, Cangzhou Central Hospital, Cangzhou 061001 Hebei, PR China. ; Meng Li:1.Department of Neurology, Cangzhou Central Hospital, Cangzhou 061001 Hebei, PR China. ;
期刊：Exosome-shuttled MYCBPAP from bone marrow mesenchymal stem cells regulates synaptic remodeling and ameliorates ischemic stroke in rats
DOI：10.1016/j.jchemneu.2023.102309
影响因子 ：2.8
引用产品： 大鼠骨髓间充质干细胞 , 大鼠骨髓间充质干细胞完全培养基 , 大鼠皮层神经元细胞 , DMEM无糖 培养基 , Annexin V-FITC/ PI荧光双染细胞凋亡检测试剂盒
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Apelin-13 attenuates early brain injury through inhibiting inflammation and apoptosis in rats after experimental subarachnoid hemorrhage (2001-02-20)
作者：Xiaoyan Shen:1.Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, Jiangsu Province, China. ; 2.Department of Neurosurgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, No. 160, Pujian Road, Pudong District, Shanghai, 200127, China. ; Guiqiang Yuan:1.Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, Jiangsu Province, China. ; Bing Li:1.Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, Jiangsu Province, China. ; 2.Department of Neurosurgery, Yancheng City No. 1 People's Hospital, The Fourth Affiliated Hospital of Nantong University, Yancheng, Jiangsu Province, China. ; Cheng Cao:1.Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affili
期刊：Apelin-13 attenuates early brain injury through inhibiting inflammation and apoptosis in rats after experimental subarachnoid hemorrhage
DOI：10.1007/s11033-021-07028-y
影响因子 ：2.8
引用产品： 大鼠皮层神经元细胞 , 大鼠皮层神经元细胞完全培养基 , BV2 细胞 , MEM (含NEAA) 培养基
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Nucleotide-binding oligomerization domain protein 1 enhances oxygen-glucose deprivation and reperfusion injury in cortical neurons via activation of endoplasmic reticulum stress-mediated autophagy (2009-00-20)
作者：Xiande Ma:1.Teaching and Experiment Center, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, People's Republic of China; Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, People's Republic of China. ; Wei Zhang:1.Third Department of Encephalopathy rehabilitation, The First Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, People's Republic of China. ; Chang Xu:1.Department of Basic Sciences of Integrated Chinese and Western Medicine, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, People's Republic of China. ; Shuangshuang Zhang:1.Department of Basic Sciences of Integrated Chinese and Western Medicine, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, People's Republic of China. ; Jiaxiu Zhao:1.Department of Basic Sciences of Integrated Chinese and Western Medicine, Liaoni
期刊：Nucleotide-binding oligomerization domain protein 1 enhances oxygen-glucose deprivation and reperfusion injury in cortical neurons via activation of endoplasmic reticulum stress-mediated autophagy
DOI：10.1016/j.yexmp.2020.104525
影响因子 ：3.6
引用产品： 大鼠皮层神经元细胞
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Involvement of brain-gut axis in treatment of cerebral infarction by β-asaron and paeonol (2002-08-20)
作者：Xiaogang He:1.Department of Neurology, Kunshan Hospital of Traditional Chinese Medicine, Affiliated to Nanjing University of Traditional Chinese Medicine, Kunshan, 215300, PR China. Electronic address: 13962641937@163.com. ; Qiufang Cai:1.Department of Neurology, Kunshan Hospital of Traditional Chinese Medicine, Affiliated to Nanjing University of Traditional Chinese Medicine, Kunshan, 215300, PR China. ; Jianxiang Li:1.Department of Neurology, Nanjing Hospital of Traditional Chinese Medicine, Affiliated to Nanjing University of Traditional Chinese Medicine, Nanjing, 210004, PR China. ; Weifeng Guo:1.First Clinical Medical College of Nanjing University of Traditional Chinese Medicine. Nanjing, 210004, PR China. ;
期刊：Involvement of brain-gut axis in treatment of cerebral infarction by β-asaron and paeonol
DOI：10.1016/j.neulet.2017.12.036
影响因子 ：2.5
引用产品： 大鼠皮层神经元细胞
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ICAM-5/Telencephalin Is a Functional Entry Receptor for Enterovirus D68 (2011-06-20)
作者：Wei Wei:1.First Hospital of Jilin University, Institute of Virology and AIDS Research, Changchun, Jilin Province 130021, China. Electronic address: wwei6@jlu.edu.cn. ; Haoran Guo:1.First Hospital of Jilin University, Institute of Virology and AIDS Research, Changchun, Jilin Province 130021, China. ; Junliang Chang:1.First Hospital of Jilin University, Institute of Virology and AIDS Research, Changchun, Jilin Province 130021, China. ; Yingzi Yu:1.Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, Ministry of Education), Second Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310058, China. ; Guanchen Liu:1.First Hospital of Jilin University, Institute of Virology and AIDS Research, Changchun, Jilin Province 130021, China. ; Nannan Zhang:1.First Hospital of Jilin University, Institute of Virology and AIDS Research, Changchun, Jilin Province 130021, China. ; Stephen H Willard:1.Department of Molecular Microbiology and Immunology, Johns Hopk
期刊：ICAM-5/Telencephalin Is a Functional Entry Receptor for Enterovirus D68
DOI：10.1016/j.chom.2016.09.013
影响因子 ：30.3
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