产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
产品概述
人支气管上皮细胞
Cat NO.: CP-H009
-
产品名称：人支气管上皮细胞
-
组织来源：支气管组织
-
产品规格：5×10⁵Cells/T25培养瓶
-
细胞简介：
人支气管上皮细胞分离自支气管组织；支气管(Bronchi)，是指由气管分出的各级分枝，由气管分出的一级支气管，即左、右主支气管。左主支气管与右主支气管相比较，前者较细长，走向倾斜；后者较粗短，走向较前者略直，所以经气管堕入的异物多进入右主支气管。支气管和气管还有以区别就是，气管是以“C”型的气管软骨为支架，而支气管不是。支气管上皮是气道与外界环境接触的第一道防线，不仅是各种病原体、炎症介质作用的靶细胞，还作为效应细胞合成、释放多种炎性介质和细胞因子，从而参与气道炎症及免疫反应。支气管上皮细胞在哮喘、慢性阻塞性肺疾病、肺癌、肺囊性纤维化以及一些感染性呼吸道疾病的发病机制中均起着重要的作用。体外培养的原代支气管上皮细胞因与体内组织在形态结构和功能活动上存在很大的相似性，因此，在基础及临床研究中极为重要。
-
方法简介：
普诺赛实验室分离的人支气管上皮细胞采用链霉蛋白酶-中性蛋白酶混合消化法结合机械刮刷并通过上皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
-
质量检测：
普诺赛实验室分离的人支气管上皮细胞经PCK免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
-
培养信息：
包被条件 鼠尾胶原Ⅰ(2-5μg/cm2) 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H009 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 上皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 人支气管上皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
-
Characterization of a pangolin SARS-CoV-2-related virus isolate that uses the human ACE2 receptor (2024-03-08)
作者：Luo-Yuan Xia:1.Institute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China. ; 2.State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China. ; Xue-Feng Wang:1.Changchun Veterinary Research Institute, Changchun, 130122, China. ; Xiao-Ming Cui:1.State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China. ; 2.Research Unit of Discovery and Tracing of Natural Focus Diseases, Chinese Academy of Medical Sciences, Beijing, 100071, China. ; Yi-Ming Zhang:1.Changchun Veterinary Research Institute, Changchun, 130122, China. ; 2.College of Life Sciences, Shandong Normal University, Jinan, 250014, China. ; Zhen-Fei Wang:1.Institute of EcoHealth, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China. ; En-Tao Li:1.Changchun Veterinary Research Institute, Changchun,
期刊：Characterization of a pangolin SARS-CoV-2-related virus isolate that uses the human ACE2 receptor
影响因子 ：9.1
引用产品： 人支气管上皮细胞 , 人支气管上皮细胞完全培养基
-
Triptolide restrains the growth, invasion, stemness, and glycolysis of non-small cell lung cancer cells by PFKFB2-mediated PI3K/AKT pathway (2024-01-07)
作者：Jiankun Ren:1.Nursing School, Hebi Polytechnic, Hebi, Henan, China. ; Songwei Zhao:1.Nursing School, Hebi Polytechnic, Hebi, Henan, China. ; Junyu Lai:1.Department of Cardiology, Affiliated Hospital of Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, China. ;
期刊：Triptolide restrains the growth, invasion, stemness, and glycolysis of non-small cell lung cancer cells by PFKFB2-mediated PI3K/AKT pathway
影响因子 ：3.0
引用产品： 人支气管上皮细胞 , A549 [A-549] 细胞
-
miR-34c-5p inhibited fibroblast proliferation, differentiation and epithelial-mesenchymal transition in benign airway stenosis via MDMX/p53 pathway (2024-02-20)
作者：Jinmei Wei:1.Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Guangxi Medical University, Nanning, China. ; Yan Chen:1.Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Guangxi Medical University, Nanning, China. ; Tingmei Feng:1.Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Guangxi Medical University, Nanning, China. ; Yuihui Wei:1.Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Guangxi Medical University, Nanning, China. ; Caizhen Yang:1.Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Guangxi Medical University, Nanning, China. ; Changwen Zhang:1.Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Guangxi Medical University, Nanning, China. ; Wentao Li:1.Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Guangx
期刊：miR-34c-5p inhibited fibroblast proliferation, differentiation and epithelial-mesenchymal transition in benign airway stenosis via MDMX/p53 pathway
DOI：10.1007/s10142-024-01317-y
影响因子 ：2.9
引用产品： 人支气管上皮细胞 , 人支气管成纤维细胞 , 293T [HEK-293T] 细胞
-
The m6 RNA methylation regulator KIAA1429 is associated with autophagy‐mediated drug resistance in lung cancer (2024-03-15)
作者：Bo Ma:1.Department of General Thoracic Surgery General Hospital of Ningxia Medical University Yinchuan China. ; Lei Xiu:1.Department of Thoracic and Cardiac Surgery General Hospital of Ningxia Medical University Yinchuan China. ; Lili Ding:1.Department of Obstetrics and Gynecology Examination General Hospital of Ningxia Medical University Yinchuan China. ;
期刊：The m6 RNA methylation regulator KIAA1429 is associated with autophagy‐mediated drug resistance in lung cancer
影响因子 ：2.7
-
Cross-species infection potential of avian influenza H13 viruses isolated from wild aquatic birds to poultry and mammals (2023-03-13)
作者：Weiyang Sun:1.Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Chang'chun, People's Republic of China. ; Menglin Zhao:1.Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Chang'chun, People's Republic of China. ; Zhijun Yu:1.Poultry Institute, Shandong Academy of Agricultural Sciences, Ji'nan, People's Republic of China. ; Yuanguo Li:1.Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Chang'chun, People's Republic of China. ; Xinghai Zhang:1.Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Chang'chun, People's Republic of China. ; Na Feng:1.Key Laboratory of Jilin Province fo
期刊：Cross-species infection potential of avian influenza H13 viruses isolated from wild aquatic birds to poultry and mammals
DOI：10.1080/22221751.2023.2184177
影响因子 ：13.2
-
IL-25 induces airway remodeling in asthma by orchestrating the phenotypic changes of epithelial cell and fibrocyte (2023-08-27)
作者：Xiujuan Yao:1.Department of Respiratory and Critical Care Medicine, Beijing Tongren Hospital, Capital Medical University, No.2, Xinanhuan Road, Yizhuang District, Beijing, 100176, China. ; Qinglin Chen:1.Department of Respiratory and Critical Care Medicine, Beijing Tongren Hospital, Capital Medical University, No.2, Xinanhuan Road, Yizhuang District, Beijing, 100176, China. ; Xiangdong Wang:1.Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China. ; 2.Key Laboratory of Otolaryngology Head and Neck Surgery of Ministry of Education of China, Beijing Institute of Otolaryngology, No. 17, Hougou Hutong, Dongcheng District, Beijing, 100005, China. ; Xiaofang Liu:1.Department of Respiratory and Critical Care Medicine, Beijing Tongren Hospital, Capital Medical University, No.2, Xinanhuan Road, Yizhuang District, Beijing, 100176, China. xfliutrhos@163.com. ; Luo Zhang:1.Department of Otolaryngology Head and Neck Surgery, Beij
期刊：IL-25 induces airway remodeling in asthma by orchestrating the phenotypic changes of epithelial cell and fibrocyte
DOI：10.1186/s12931-023-02509-z
影响因子 ：5.8
引用产品： 人支气管上皮细胞
-
IGF2BP1 enhances the stability of SIK2 mRNA through m6A modification to promote non-small cell lung cancer progression (2023-10-12)
作者：Yan Xu:1.The Second Department of Thoracic Oncology, Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan Province, PR China. ; Li Xu:1.The Second Department of Thoracic Oncology, Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan Province, PR China. ; Yi Kong:1.The Second Department of Thoracic Oncology, Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan Province, PR China. ; Kang Li:1.The Second Department of Thoracic Oncology, Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan Province, PR China. ; Jia Li:1.The Second Department of Thoracic Oncology, Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South Uni
期刊：IGF2BP1 enhances the stability of SIK2 mRNA through m6A modification to promote non-small cell lung cancer progression
DOI：10.1016/j.bbrc.2023.10.045
影响因子 ：3.1
引用产品： A549 [A-549] 细胞 , NCI-H1299 细胞 , HCC827 细胞 , NCI-H1975 细胞 , 人支气管上皮细胞
-
Serum Hsa_circ_0005962 Is A Prognostic Biomarker of Paclitaxel Resistance in Nonsmall Cell Lung Cancer Treatment (2023-10-19)
作者：Chenliang Wang:1.Department of Pathology, The First People's Hospital of Jiujiang City, Jiujiang, Jiangxi, China. ; Shaofeng Xia:1.Department of Thoracic Surgery, The First People's Hospital of Jiujiang City, Jiujiang, Jiangxi, China. ;
期刊：Serum Hsa_circ_0005962 Is A Prognostic Biomarker of Paclitaxel Resistance in Nonsmall Cell Lung Cancer Treatment
影响因子 ：2.6
引用产品： 人支气管上皮细胞 , A549 [A-549] 细胞 , NCI-H460 [H460] 细胞
-
Propofol-mediated circ_0000735 downregulation restrains tumor growth by decreasing integrin-β1 expression in non-small cell lung cancer (2023-01-31)
作者：Lihui Zhang:1.Department of Anesthesiology, Hulunbeier Municipal People's Hospital (Hulunbuir Hospital Affiliated to Suzhou University Hulunbuir), Inner Mongolia, China. ; Yunli Gao:1.Department of Anesthesiology, Maanshan People's Hospital, Maanshan, Anhui, China. ; Yue Li:1.Department of Anesthesiology, Hulunbeier Municipal People's Hospital (Hulunbuir Hospital Affiliated to Suzhou University Hulunbuir), Inner Mongolia, China. ; Xinying Li:1.Department of Anesthesiology, Hulunbeier Municipal People's Hospital (Hulunbuir Hospital Affiliated to Suzhou University Hulunbuir), Inner Mongolia, China. ; Haixia Gong:1.Department of Anesthesiology, First Affiliated Hospital of Nanchang University, No. 17 Yongwaizhengjie Street, Donghu District, Nanchang, Jiangxi Province, 330006 China. ;
期刊：Propofol-mediated circ_0000735 downregulation restrains tumor growth by decreasing integrin-β1 expression in non-small cell lung cancer
影响因子 ：2.1
引用产品： 人支气管上皮细胞 , NCI-H1299 细胞 , A549 [A-549] 细胞 , 293T [HEK-293T] 细胞 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100×
-
METTL3 promotes non-small cell lung cancer (NSCLC) cell proliferation and colony formation in a m6A-YTHDF1 dependent way (2022-08-25)
作者：Xuejun Dou:1.Department of Thoracic Surgery, Aerospace Center Hospital, Beijing, China. douxj08@163.com. ; Zhiyuan Wang:1.Department of Thoracic Surgery, Aerospace Center Hospital, Beijing, China. ; Weiqiang Lu:1.Department of Thoracic Surgery, Aerospace Center Hospital, Beijing, China. ; Libin Miao:1.Department of Thoracic Surgery, Aerospace Center Hospital, Beijing, China. ; Yuefeng Zhao:1.Department of Thoracic Surgery, Aerospace Center Hospital, Beijing, China. ;
期刊：METTL3 promotes non-small cell lung cancer (NSCLC) cell proliferation and colony formation in a m6A-YTHDF1 dependent way
DOI：10.1186/s12890-022-02119-3
影响因子 ：3.3
引用产品： A549 [A-549] 细胞 , NCI-H1299 细胞 , HCC827 细胞 , NCI-H1975 细胞 , 人支气管上皮细胞 , 青霉素-链霉素溶液 (双抗)，100×
-
Propofol suppresses lung cancer tumorigenesis by modulating the circ-ERBB2/miR-7-5p/FOXM1 axis (2001-01-20)
作者：Jie Gao:1.Department of Anesthesiology, Zhengzhou University People's Hospital, Henan Provincial People's Hospital, Zhengzhou, China. ; Chengzhi Ding:1.Department of Thoracic Surgery, Henan Provincial Chest Hospital, Zhengzhou, China. ; Junhui Zhou:1.Department of Anesthesiology, Henan Provincial Chest Hospital, Zhengzhou, China. ; Gang Wu:1.Department of Cardiovascular Surgery, Henan Provincial Chest Hospital, Zhengzhou, China. ; Zongmao Han:1.Department of Cardiology, People's Hospital of Zhengzhou University, People's Hospital, Henan Provincial People's Hospital, Zhengzhou, China. ; Jianchao Li:1.Department of Extracorporeal Circulation, Zhengzhou University People's Hospital, Henan Provincial People's Hospital, Zhengzhou, China. ; Feilong Hei:1.Department of Extracorporeal Circulation, Zhengzhou University People's Hospital, Henan Provincial People's Hospital, Zhengzhou, China. ;
期刊：Propofol suppresses lung cancer tumorigenesis by modulating the circ-ERBB2/miR-7-5p/FOXM1 axis
影响因子 ：2.9
引用产品： 人支气管上皮细胞
-
Identification of circ_0058357 as a regulator in non-small cell lung cancer cells resistant to cisplatin by miR-361-3p/ABCC1 axis (2009-01-20)
作者：Dan Chu:1.Department of Respiratory Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ; Pengpeng Li:1.Cancer Gamma Knife Center, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ; Yameng Li:1.Department of Respiratory Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ; Jiang Shi:1.Department of Respiratory Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ; Siyuan Huang:1.Department of Respiratory Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ; Pengfei Jiao:1.Department of Respiratory Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ;
期刊：Identification of circ_0058357 as a regulator in non-small cell lung cancer cells resistant to cisplatin by miR-361-3p/ABCC1 axis
影响因子 ：2.9
引用产品： 人支气管上皮细胞 , A549 [A-549] 细胞 , A549/DDP 细胞 , NCI-H1299 细胞
-
ICAM-5/Telencephalin Is a Functional Entry Receptor for Enterovirus D68 (2011-06-20)
作者：Wei Wei:1.First Hospital of Jilin University, Institute of Virology and AIDS Research, Changchun, Jilin Province 130021, China. Electronic address: wwei6@jlu.edu.cn. ; Haoran Guo:1.First Hospital of Jilin University, Institute of Virology and AIDS Research, Changchun, Jilin Province 130021, China. ; Junliang Chang:1.First Hospital of Jilin University, Institute of Virology and AIDS Research, Changchun, Jilin Province 130021, China. ; Yingzi Yu:1.Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, Ministry of Education), Second Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310058, China. ; Guanchen Liu:1.First Hospital of Jilin University, Institute of Virology and AIDS Research, Changchun, Jilin Province 130021, China. ; Nannan Zhang:1.First Hospital of Jilin University, Institute of Virology and AIDS Research, Changchun, Jilin Province 130021, China. ; Stephen H Willard:1.Department of Molecular Microbiology and Immunology, Johns Hopk
期刊：ICAM-5/Telencephalin Is a Functional Entry Receptor for Enterovirus D68
DOI：10.1016/j.chom.2016.09.013
影响因子 ：30.3
FAQs
Q：{{item.question}}
A：
产品资料
识别码示意图
