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大鼠脑微血管内皮细胞
Rat Brain Microvascular Endothelial Cells
货号：CP-R108
规格：5×10⁵Cells/T25培养瓶
价格： ￥3880
培养基货号： CM-R108
产品概述
大鼠脑微血管内皮细胞
Cat NO.: CP-R108
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产品名称：大鼠脑微血管内皮细胞
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英文简称：BMVECs
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组织来源：脑组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
大鼠脑微血管内皮细胞分离自脑组织；脑微血管内皮细胞是血脑屏障的主要组成成分，它是组成脑微血管腔面单层扁平上皮样细胞，能够限制可溶性物质和细胞等从血液进入大脑，大脑微血管内皮细胞与外周内皮细胞相比具有一些相同特性。它所产生和分泌的生物活性物质对维持血管张力、调节血压、抗血栓形成等有重要作用，在脑血管疾病的发病机制中有重要病理生理学意义。与外周内皮细胞相同，大脑微血管内皮细胞表面表达细胞粘附分子，调控白细胞进入大脑。由于微血管内皮细胞的器官特异性，内皮细胞通常取源于疾病研究的相关组织。其主要特征如下：①脑微血管内皮细胞存在许多细胞间紧密连接，产生很高的跨内皮阻抗，延迟细胞旁的通量；②脑微血管内皮细胞缺乏内皮细胞的窗孔结构，其液相物质胞饮水平较低；③脑微血管内皮细胞具有不对称定位酶和载体介导转运系统，从而产生“两极分化”的表现型。
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方法简介：
普诺赛实验室分离的大鼠脑微血管内皮细胞采用胶原酶-中性蛋白酶混合消化法结合密度梯度离心法、最后通过内皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的大鼠脑微血管内皮细胞经CD31免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 PLL（0.1mg/ml）或明胶（0.1%） 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-R108 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 内皮细胞样 传代特性 可传1-2代；不建议多次传代 消化液 0.25%胰蛋白酶 大鼠脑微血管内皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Baicalin Reduces Immune Cell Infiltration by Inhibiting Inflammation and Protecting Tight Junctions in Ischemic Stroke Injury (2023-01-20)
作者：Zhiyuan Hao:1.Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, P. R. China. ; Zheyu Zhang:1.Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, Hunan, P. R. China. ; Yuhang Zhao:1.Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, P. R. China. ; Dongsheng Wang:1.Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, P. R. China. ;
期刊：Baicalin Reduces Immune Cell Infiltration by Inhibiting Inflammation and Protecting Tight Junctions in Ischemic Stroke Injury
影响因子 ：5.7
引用产品： 大鼠脑微血管内皮细胞
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Bombyx batryticatus extract activates coagulation factor Ⅻ to promote angiogenesis in rats with cerebral ischemia/reperfusion injury (2023-08-25)
作者：Shan-Shan Wang:1.Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, China. ; Hao Xu:1.Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, China. ; An-Qi Ge:1.The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, China. ; Kai-Lin Yang:1.Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, China. ; Qi He:1.People's Hospital of Ningxiang City, Ningxiang, China. ; Jin-Wen Ge:1.Key Laboratory of
期刊：Bombyx batryticatus extract activates coagulation factor Ⅻ to promote angiogenesis in rats with cerebral ischemia/reperfusion injury
影响因子 ：5.4
引用产品： 大鼠脑微血管内皮细胞 , 293 [HEK-293] 细胞
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Transcription Factor HEY1 Improves Brain Vascular Endothelial Cell Function and Alleviates Ischemic Stroke by Upregulating NOTCH3 (2003-02-20)
作者：Tingting Zhu:1.Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, 610041, Sichuan, People's Republic of China. ; 2.Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, Sichuan University, Chengdu, Sichuan, People's Republic of China. ; Hongxi Chen:1.Department of Neurology, West China Hospital of Sichuan University, Chengdu, 610041, Sichuan, People's Republic of China. ; Cuihong He:1.Chengdu Women and Children's Central Hospital, University of Electronic Science and Technology, Chengdu, 610015, Sichuan, People's Republic of China. ; Xiaojuan Liu:1.Department of Neurology, Xiangya Hospital of Central South University, Changsha, 410008, Hunan, People's Republic of China. liuxiaojuan1980@126.com. ;
期刊：Transcription Factor HEY1 Improves Brain Vascular Endothelial Cell Function and Alleviates Ischemic Stroke by Upregulating NOTCH3
DOI：10.1007/s11064-022-03544-w
影响因子 ：4.4
引用产品： 大鼠脑微血管内皮细胞
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d-allose protects brain microvascular endothelial cells from hypoxic/reoxygenated injury by inhibiting endoplasmic reticulum stress (2022-12-05)
作者：Min Zhang:1.College of Life Sciences, Northwest University, Xi'an, Shaanxi, China; Department of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi'an, Shaanxi, China. ; Yi-Hao Fu:1.Department of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi'an, Shaanxi, China. ; Yao-Wen Luo:1.Department of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi'an, Shaanxi, China. ; Mao-Rong Gou:1.Department of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi'an, Shaanxi, China. ; Lei Zhang:1.Department of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi'an, Shaanxi, China. ; Zhou Fei:1.Department of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi'an, Shaanxi, China. Electronic address: feizhou@fmmu.edu.cn. ; Da-Kuan Gao:1.Department of Neurosurgery, Xijing Hospital, Air Force Medical University, Xi'an, Shaanxi, China. Electronic address: syjgdk@126.com. ;
期刊：d-allose protects brain microvascular endothelial cells from hypoxic/reoxygenated injury by inhibiting endoplasmic reticulum stress
DOI：10.1016/j.neulet.2022.137000
影响因子 ：3.2
引用产品： 大鼠脑微血管内皮细胞
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Telomerase reverse transcriptase promotes angiogenesis in neonatal rats after hypoxic-ischemic brain damage (2022-10-21)
作者：Jiao Li:1.Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, China. ; Yi Feng:1.Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, China. ; Jing Zhao:1.Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, China. ; Zhi Fang:1.Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, China. ; Haiting Liu:1.Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, China. ;
期刊：Telomerase reverse transcriptase promotes angiogenesis in neonatal rats after hypoxic-ischemic brain damage
影响因子 ：3.1
引用产品： 大鼠脑微血管内皮细胞
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Role of p75 neurotrophin receptor in neuronal autophagy in intracerebral hemorrhage in rats through the mTOR signaling pathway (2001-00-20)
作者：Lei Wang:1.Department of emergency medicine, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, P.R. China. ; Meilei Tian:1.Department of emergency medicine, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, P.R. China. ; Yugui Hao:1.Department of emergency medicine, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, P.R. China. ;
期刊：Role of p75 neurotrophin receptor in neuronal autophagy in intracerebral hemorrhage in rats through the mTOR signaling pathway
DOI：10.1080/15384101.2019.1711318
影响因子 ：4.3
引用产品： 大鼠脑微血管内皮细胞 , 大鼠神经星形胶质细胞
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