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产品概述
大鼠神经星形胶质细胞
Cat NO.: CP-R137
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产品名称：大鼠神经星形胶质细胞
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组织来源：脑组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
大鼠神经星形胶质细胞分离自脑组织；大脑分左右两个半球，大脑皮质(灰质)覆盖着每个大脑半球的大部分，它是神经元胞体集中的地方。内部则是由神经纤维或髓鞘构成的白质。每一个半球都有三个面，即外侧面(约占整个皮质面积的1/3)、内侧面和底面(占2/3的面积)；半球表面有很多深浅不等的沟或裂，沟或裂之间的隆起叫回，它们大大增加了大脑的表面积；大脑外侧面重要的沟、裂有大脑外侧裂、顶枕裂和中央沟。由于三沟裂之界隔，使大脑皮质组分为额叶、顶叶、颞叶、枕叶四大部分。神经星形胶质细胞，是哺乳动物脑内分布最广泛的一类细胞，也是胶质细胞中体积最大的一种。用经典的金属浸镀技术(银染色)显示此类胶质细胞呈星形，从胞体发出许多长而分支的突起，伸展充填在神经细胞的胞体及其突起之间，起支持和分隔神经细胞的作用。细胞突起的末端常膨大形成脚板或称终足，有些脚板贴附在邻近的毛细血管壁上，因此这些脚板又被称为血管足或血管周足，靠近脑脊髓表面的脚板则附着在软膜内表面，彼此连接构成胶质界膜。细胞刚分离时呈圆形，24h后大部分细胞贴壁，均匀分布于瓶底，部分细胞伸出细小突起，培养4-5d后细胞数量明显增多，呈扁平、多角形，胞质丰富且核浆较少，细胞核呈椭圆形，偏于胞体一侧。神经星形胶质细胞是中枢神经系统的重要细胞组成，不仅具有支持功能，还能够合成及分泌多种神经活性物质，在维持神经元内外环境、生存、迁移、免疫调节、信号转导、轴突生长及功能整合等方面具有重要作用。
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方法简介：
普诺赛实验室分离的大鼠神经星形胶质细胞采用酶消化法结合差速贴壁法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的大鼠神经星形胶质细胞经GFAP免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-R137 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 梭形、多角形 传代特性 可传5代左右；3代以内状态最佳 消化液 0.25%胰蛋白酶 大鼠神经星形胶质细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Integration of genetically engineered virus nanofibers and fibrin to form injectable fibrous neuron-rich hydrogels and enable neural differentiation (2023-01-04)
作者：Yingfan Chen:1.School of Materials Science and Engineering, Zhejiang University, Zheda Road 38, Hangzhou 310027, Zhejiang, P. R. China. ; Xiangyu Liu:1.School of Materials Science and Engineering, Zhejiang University, Zheda Road 38, Hangzhou 310027, Zhejiang, P. R. China. ; Mingying Yang:1.Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, China. yangm@zju.edu.cn. ; Weilian Sun:1.Department of Periodontology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310009, P. R. China. weiliansun@zju.edu.cn. ; Chuanbin Mao:1.School of Materials Science and Engineering, Zhejiang University, Zheda Road 38, Hangzhou 310027, Zhejiang, P. R. China. ; 2.Department of Chemistry & Biochemistry, Stephenson Life Science Research Center, Institute for Biomedical Engineering Science and Technology, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019-5251, USA. maophage@gm
期刊：Integration of genetically engineered virus nanofibers and fibrin to form injectable fibrous neuron-rich hydrogels and enable neural differentiation
影响因子 ：7.0
引用产品： 大鼠神经星形胶质细胞
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Overexpression of miR-124 in astrocyte improves neurological deficits in rat with ischemic stroke via DLL4 modulation (2023-10-15)
作者：Xiaoqin Guo:1.Provincial and Ministerial Co-founded Collaborative Innovation Center of Rehabilitation Technology, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China. ; Pingli Jiang:1.Provincial and Ministerial Co-founded Collaborative Innovation Center of Rehabilitation Technology, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China. ; Meihua Pan:1.National-Local Joint Engineering Research Center of Rehabilitation Medicine Technology, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China. ; Yanyi Ding:1.National-Local Joint Engineering Research Center of Rehabilitation Medicine Technology, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China. ; Yanting Lin:1.National-Local Joint Engineering Research Center of Rehabilitation Medicine Technology, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China. ; Tao Jiang:1.Fujian Key Laboratory of Cognitive
期刊：Overexpression of miR-124 in astrocyte improves neurological deficits in rat with ischemic stroke via DLL4 modulation
DOI：10.1016/j.expneurol.2023.114571
影响因子 ：5.3
引用产品： 大鼠神经星形胶质细胞
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Upregulation of TRPC6 inhibits astrocyte activation and proliferation after spinal cord injury in rats by suppressing AQP4 expression (2022-09-15)
作者：Jiajun Cai:1.Department of Orthopedics, The First Affiliated Hospital, Jinzhou Medical University, Jinzhou 121000, China. ; Jundong Kong:1.Department of Orthopedics, The First Affiliated Hospital, Jinzhou Medical University, Jinzhou 121000, China. ; Song Ma:1.Department of Orthopedics, The First Affiliated Hospital, Jinzhou Medical University, Jinzhou 121000, China. ; Yaozu Ban:1.Department of Orthopedics, The First Affiliated Hospital, Jinzhou Medical University, Jinzhou 121000, China. ; Jian Li:1.Department of Orthopedics, The First Affiliated Hospital, Jinzhou Medical University, Jinzhou 121000, China. Electronic address: jk1403928214@163.com. ; Zhongkai Fan:1.Department of Orthopedics, The First Affiliated Hospital, Jinzhou Medical University, Jinzhou 121000, China. Electronic address: fanzk_ln@163.com. ;
期刊：Upregulation of TRPC6 inhibits astrocyte activation and proliferation after spinal cord injury in rats by suppressing AQP4 expression
DOI：10.1016/j.brainresbull.2022.09.010
影响因子 ：3.7
引用产品： 大鼠神经星形胶质细胞
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Preparation of gastrodin-modified dendrimer-entrapped gold nanoparticles as a drug delivery system for cerebral ischemia–reperfusion injury (2022-11-21)
作者：Wenqiang Huang:1.Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming, China. ; Lanlin Wang:1.Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming, China. ; Yanghong Zou:1.Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming, China. ; Xiangqian Ding:1.Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming, China. ; Xin Geng:1.Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming, China. ; Jinghui Li:1.Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming, China. ; Hexiang Zhao:1.Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming, China. ; Renli Qi:1.Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming, China. ; Shipeng Li:1.Department of Neurosurgery, First Affiliat
期刊：Preparation of gastrodin-modified dendrimer-entrapped gold nanoparticles as a drug delivery system for cerebral ischemia–reperfusion injury
影响因子 ：3.4
引用产品： 大鼠神经星形胶质细胞 , 大鼠下丘脑神经元细胞
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Role of p75 neurotrophin receptor in neuronal autophagy in intracerebral hemorrhage in rats through the mTOR signaling pathway (2001-00-20)
作者：Lei Wang:1.Department of emergency medicine, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, P.R. China. ; Meilei Tian:1.Department of emergency medicine, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, P.R. China. ; Yugui Hao:1.Department of emergency medicine, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, P.R. China. ;
期刊：Role of p75 neurotrophin receptor in neuronal autophagy in intracerebral hemorrhage in rats through the mTOR signaling pathway
DOI：10.1080/15384101.2019.1711318
影响因子 ：4.3
引用产品： 大鼠脑微血管内皮细胞 , 大鼠神经星形胶质细胞
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