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![293 [HEK-293] (人胚肾细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0001-1-1.jpg)
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产品概述
| 名称 | 293 [HEK-293] (人胚肾细胞) (STR鉴定正确) |
| 别称 | Hek293; HEK-293; HEK 293; HEK:293; 293; 293 HEK; Human Embryonic Kidney 293 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:4-1:8 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 该细胞贴壁松散，操作时请尽量轻柔；换液时需预热培养基；收货如有大块脱落的细胞团，为正常现象，请按照收货注意事项处理。 |
| 背景描述 | 293 [HEK-293]细胞是剪切过的人腺病毒5(Ad5)转染的人胚肾细胞形成的永生化细胞，293 [HEK-293]细胞包含并表达转染的Ad5基因。早期报道中指出，293 [HEK-293]细胞基因组中含有腺病毒5(Ad5)基因组的左侧端和右侧端的DNA，但是现在明确了只存在其左侧端的DNA。经过对Ad5的插入点的克隆测序发现，Ad5的1-4344位线性核苷酸整合入293 [HEK-293]细胞19号染色体(19q13.2)。293 [HEK-293]细胞为人类腺病毒载体扩增的宿主，可表达异常的玻连蛋白的细胞表面受体，由整合素β1亚单位和玻连蛋白受体α-v亚单位组成。 |
| 年龄（性别） | 胎儿 |
| 组织来源 | 肾；以腺病毒5DNA进行转化 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | BSL-2 |
| 倍增时间 | ~24-30 hours |
| 致瘤性 | Yes, forms tumors in nude mice. |
| 受体表达情况 | vitronectin |
| 保藏机构 | ATCC; CRL-1573 ATCC： PTA-4488 DSMZ： ACC-305 ECACC： 85120602 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 19 D3S1358 15,17 D5S818 8 D7S820 11,12 D8S1179 12,14 D13S317 12,14 D16S539 9,13 D18S51 17,18 D19S433 15,16.2,18 D21S11 28,30.2 FGA 23 PentaD 9,10 PentaE 7,15 TH01 7,9.3 TPOX 11 vWA 16,19 D6S1043 11 D12S391 19,21 D2S441 11,15 -
STR鉴定图
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参考文献
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Gelatin-Encapsulated Tetrahedral DNA Nanostructure Enhances Cellular Internalization for Treating Noise-Induced Hearing Loss (2024-02-08)
期刊：Small
影响因子 ：13.3
引用产品： 293 [HEK-293] 细胞 , VS 细胞
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Discovery of Novel Coumarin-quinolinium Derivatives as Pan-KRAS Translation Inhibitors by Targeting 5′-UTR RNA G-Quadruplexes (2024-01-25)
期刊：JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1021/acs.jmedchem.3c01773
影响因子 ：7.3
引用产品： MIA PaCa-2 细胞 , DMEM高糖 培养基 , 293 [HEK-293] 细胞 , HPAF-II 细胞 , U-87 MG 细胞 , MEM (ATCC改良) 培养基 , PANC-1 细胞 , HCT 116 细胞 , HFL1 细胞
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Supramolecular Switch for the Regulation of Antibacterial Efficacy of Near-Infrared Photosensitizer (2024-02-28)
期刊：MOLECULES
影响因子 ：4.6
引用产品： 293 [HEK-293] 细胞
-
Landscape of pathogenic mutations in premature ovarian insufficiency (2023-02-02)
期刊：NATURE MEDICINE
DOI：10.1038/s41591-022-02194-3
影响因子 ：82.9
引用产品： 293 [HEK-293] 细胞 , Hela 细胞 , CHO 细胞
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Delivery of Mrna Vaccine with 1, 2-diesters-derived Lipids Elicits Fast Liver Clearance for Safe and Effective Cancer Immunotherapy. (2023-11-21)
期刊：Advanced Healthcare Materials
影响因子 ：10.0
引用产品： B16 细胞 , RM-1 细胞 , DC2.4 细胞 , 293 [HEK-293] 细胞
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Rational synthesis of carbon dots with phosphate ester group for direct mapping of endogenous alkaline phosphatase and polarity monitoring in living cells (2023-02-28)
期刊：JOURNAL OF COLLOID AND INTERFACE SCIENCE
DOI：10.1016/j.jcis.2023.02.133
影响因子 ：9.9
引用产品： Hela 细胞 , 293 [HEK-293] 细胞 , Hep G2 细胞 , LX-2 细胞
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The novel protein complex FAPα/ITGA5 is involved in the bone destruction of apical periodontitis (2023-11-17)
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2023.128200
影响因子 ：8.2
引用产品： MC3T3-E1 Subclone 24 细胞 , RAW 264.7 细胞 , 293 [HEK-293] 细胞
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Combined Doxorubicin Mesoporous Carbon Nanospheres for Effective Tumor Lymphatic Metastasis by Multi-Modal Chemo-Photothermal Treatment in vivo (2023-08-11)
期刊：International Journal of Nanomedicine
影响因子 ：8.0
引用产品： 4T1 细胞 , 293 [HEK-293] 细胞
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Loss of renal tubular G9a benefits acute kidney injury by lowering focal lipid accumulation via CES1 (2023-04-12)
期刊：EMBO REPORTS
影响因子 ：7.7
引用产品： 293 [HEK-293] 细胞
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Low miR-182-5p Expressing Extracellular Vesicles Derived From Human Bone Marrow Stromal Cells of Subjects With Steroid-Induced Osteonecrosis of the Femoral Head Aggravate Disease Progression (2023-05-08)
期刊：JOURNAL OF BONE AND MINERAL RESEARCH
影响因子 ：6.2
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DEPDC1B-mediated USP5 deubiquitination of β-catenin promotes breast cancer metastasis by activating the wnt/β-catenin pathway (2023-08-29)
期刊：AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
DOI：10.1152/ajpcell.00249.2023
影响因子 ：5.5
引用产品： MDA-MB-231 细胞 , MDA-MB-468 细胞 , MDA-MB-157 细胞 , BT-549 细胞 , 293 [HEK-293] 细胞 , DMEM/F12 培养基 , Hs 578T细胞专用培养基
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Bombyx batryticatus extract activates coagulation factor Ⅻ to promote angiogenesis in rats with cerebral ischemia/reperfusion injury (2023-08-25)
期刊：JOURNAL OF ETHNOPHARMACOLOGY
影响因子 ：5.4
引用产品： 大鼠脑微血管内皮细胞 , 293 [HEK-293] 细胞
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Characterization of the metabolites of Eucommiae Cortex in rats provides a further insight into its estrogen-like effective substances (2023-12-20)
期刊：BIOORGANIC CHEMISTRY
DOI：10.1016/j.bioorg.2023.107052
影响因子 ：5.1
引用产品： 293 [HEK-293] 细胞
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Targeting PKD2 aggravates ferritinophagy-mediated ferroptosis via promoting autophagosome-lysosome fusion and enhances efficacy of carboplatin in lung adenocarcinoma (2023-11-10)
期刊：CHEMICO-BIOLOGICAL INTERACTIONS
影响因子 ：5.1
引用产品： A549 [A-549] 细胞 , PC-9 细胞 , 293 [HEK-293] 细胞
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Copine 7 promotes colorectal cancer proliferation through PKM2 interaction and MAPK signaling pathway (2023-07-04)
期刊：Frontiers in Oncology
影响因子 ：4.7
引用产品： 293 [HEK-293] 细胞 , HCT 116 细胞 , DLD-1 细胞 , McCoy's 5A 培养基 , 特级胎牛血清 , RPMI-1640 培养基 , DMEM高糖 培养基
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Silencing ApoC3 alleviates LPS-induced acute lung injury by inhibiting TLR signaling pathway (2023-04-10)
期刊：IMMUNOLOGIC RESEARCH
DOI：10.1007/s12026-023-09379-z
影响因子 ：4.4
引用产品： 293 [HEK-293] 细胞
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YBX2 modulates mRNA stability via interaction with YTHDF2 in endometrial cancer cells (2023-04-06)
期刊：EXPERIMENTAL CELL RESEARCH
DOI：10.1016/j.yexcr.2023.113586
影响因子 ：3.7
引用产品： Ishikawa 细胞 , 293 [HEK-293] 细胞 , Hela 细胞 , DMEM高糖 培养基
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Rapid Screen of IL-5/IL-5Rα Blocking Antibodies in the HEK293-IL-5Rα-CSF2RB Transfected Cell Line (2023-05-20)
期刊：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
影响因子 ：3.2
引用产品： 293 [HEK-293] 细胞 , 293T [HEK-293T] 细胞 , TF-1 细胞
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Inhibition of Kv7/M Channel Currents by Fangchinoline (2023-03-07)
期刊：PHARMACOLOGY
影响因子 ：3.1
引用产品： 293 [HEK-293] 细胞
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Scoparone inhibits breast cancer cell viability through the NF‑κB signaling pathway (2023-05-17)
期刊：Experimental and Therapeutic Medicine
影响因子 ：2.7
引用产品： MCF 10A 细胞 , MCF7 [MCF-7] 细胞 , MDA-MB-231 细胞 , ZR-75-1 细胞 , HCC1937 细胞 , 293 [HEK-293] 细胞
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SPI1 Mediates N-Myristoyltransferase 1 to Advance Gastric Cancer Progression via PI3K/AKT/mTOR Pathway (2023-03-17)
期刊：Canadian Journal of Gastroenterology and Hepatology
影响因子 ：2.7
引用产品： GES-1 细胞 , AGS 细胞 , HGC-27 细胞 , SNU-5 细胞 , MKN-45 细胞 , RPMI-1640 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100× , Ham's F-12 培养基 , IMDM 培养基 , 293 [HEK-293] 细胞 , MEM (ATCC改良) 培养基
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Inhibitory effect of berberine on morphine tolerance and hyperalgesia in mice (2023-08-02)
期刊：Journal of Traditional Chinese Medicine
DOI：10.19852/j.cnki.jtcm.20230802.006
影响因子 ：2.6
引用产品： HT22 细胞 , 293 [HEK-293] 细胞
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IRF1 promotes the chondrogenesis of human adipose-derived stem cells through regulating HILPDA (2023-02-21)
期刊：TISSUE & CELL
DOI：10.1016/j.tice.2023.102046
影响因子 ：2.6
引用产品： 293 [HEK-293] 细胞 , 293 [HEK-293]细胞专用培养基
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Proinflammatory Bone Marrow Mesenchymal Stem Cell-Derived Exosomal miR-150-3p Suppresses Proinflammatory Polarization of Alveolar Macrophages in Sepsis by Targeting Inhibin Subunit Beta A (2023-10-11)
期刊：JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
影响因子 ：2.3
引用产品： 293 [HEK-293] 细胞 , MEM (ATCC改良) 培养基
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Simulation guided intramolecular orthogonal reporters for dissecting cellular oxidative stress and response (2022-08-05)
期刊：Nano Today
DOI：10.1016/j.nantod.2022.101573
影响因子 ：19.0
引用产品： Hela 细胞 , A549 [A-549] 细胞 , 293 [HEK-293] 细胞
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Precise Detection on Cell–Cell Fusion by a Facile Molecular Beacon-Based Method (2022-12-01)
期刊：ANALYTICAL CHEMISTRY
DOI：10.1021/acs.analchem.2c04852
影响因子 ：8.0
引用产品： 293 [HEK-293] 细胞 , HuH-7 细胞 , MDA-MB-231 细胞 , NCI-H1299 细胞 , J774A.1 细胞
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miR-34a Regulates Lipid Droplet Deposition in 3T3-L1 and C2C12 Cells by Targeting LEF1 (2022-12-30)
期刊：Cells
影响因子 ：7.7
引用产品： 3T3-L1 细胞 , C2C12 细胞 , 293 [HEK-293] 细胞
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Delta Opioid Peptide Targets Brain Microvascular Endothelial Cells Reducing Apoptosis to Relieve Hypoxia-Ischemic/Reperfusion Injury (2022-12-23)
期刊：Pharmaceutics
DOI：10.3390/pharmaceutics15010046
影响因子 ：6.5
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Antibacterial and Antifungal Properties of a Novel Antimicrobial Peptide GK-19 and Its Application in Skin and Soft Tissue Infections Induced by MRSA or Candida albicans (2022-09-13)
期刊：Pharmaceutics
DOI：10.3390/pharmaceutics14091937
影响因子 ：6.5
引用产品： 293 [HEK-293] 细胞
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Ubiquitin ligase Triad1 promotes neurite outgrowth by inhibiting MDM2-mediated ubiquitination of the neuroprotective factor pleiotrophin (2022-08-30)
期刊：JOURNAL OF BIOLOGICAL CHEMISTRY
影响因子 ：5.5
引用产品： 293 [HEK-293] 细胞 , 293 [HEK-293]细胞专用培养基
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Exosome‑encapsulated miR‑26a attenuates aldosterone‑induced tubulointerstitial fibrosis by inhibiting the CTGF/SMAD3 signaling pathway (2022-12-12)
期刊：INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
影响因子 ：5.3
引用产品： 293 [HEK-293] 细胞
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Integrated miRNA and mRNA omics reveal dioscin suppresses migration and invasion via MEK/ERK and JNK signaling pathways in human endometrial carcinoma in vivo and in vitro (2022-12-09)
期刊：JOURNAL OF ETHNOPHARMACOLOGY
影响因子 ：5.2
引用产品： Ishikawa 细胞 , 293 [HEK-293] 细胞
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Silencing circular RNAPTPN12 promoted the growth of keloid fibroblasts by activating Wnt signaling pathway via targeting microRNA-21-5p (2001-02-20)
期刊：Bioengineered
DOI：10.1080/21655979.2022.2029108
影响因子 ：4.9
引用产品： 293 [HEK-293] 细胞
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Whole genome sequencing identifies a deletion mutation in the unknown-functional KCNG2 from familial sick sinus syndrome (2004-02-20)
期刊：PHYSIOLOGICAL GENOMICS
DOI：10.1152/physiolgenomics.00132.2021
影响因子 ：4.6
引用产品： 293 [HEK-293] 细胞
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Bta-miR-106b Regulates Bovine Mammary Epithelial Cell Proliferation, Cell Cycle, and Milk Protein Synthesis by Targeting the CDKN1A Gene (2022-12-07)
期刊：Genes
影响因子 ：4.1
引用产品： 293 [HEK-293] 细胞
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Effects of Lycium barbarum glycopeptide on renal and testicular injury induced by di(2-ethylhexyl) phthalate (2004-02-20)
期刊：CELL STRESS & CHAPERONES
DOI：10.1007/s12192-022-01266-0
影响因子 ：3.8
引用产品： 293 [HEK-293] 细胞 , HK-2 细胞
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KLF5-mediated aquaporin 3 activated autophagy to facilitate cisplatin resistance of gastric cancer (2022-09-20)
期刊：IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
DOI：10.1080/08923973.2022.2122498
影响因子 ：3.7
引用产品： 293 [HEK-293] 细胞 , 青霉素-链霉素溶液 (双抗)，100× , MEM (ATCC改良) 培养基
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Cancer stem cell-like cells-derived exosomal lncRNA CDKN2B-AS1 promotes biological characteristics in thyroid cancer via miR-122-5p/P4HA1 axis (2022-12-14)
期刊：Regenerative Therapy
DOI：10.1016/j.reth.2022.11.005
影响因子 ：3.7
引用产品： RPMI-1640 培养基 , 特级胎牛血清 , 293 [HEK-293] 细胞 , MEM (ATCC改良) 培养基
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Progesterone Changes the Pregnancy-Induced Adaptation of Cardiomyocyte Kv2.1 Channels via MicroRNA-29b (2004-02-20)
期刊：Cardiovascular Therapeutics
影响因子 ：3.1
引用产品： 293 [HEK-293] 细胞 , H9c2 (2-1) 细胞
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The anticancer effect of EGFR-targeting artificial microRNA controlled by SLPI promoter in nasopharyngeal carcinoma (2022-10-25)
期刊：JOURNAL OF CLINICAL LABORATORY ANALYSIS
影响因子 ：3.1
引用产品： 293 [HEK-293] 细胞 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100× , RPMI-1640 培养基 , DMEM高糖 培养基 , MEM (ATCC改良) 培养基
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LPS-induced PTGS2 manipulates the inflammatory response through trophoblast invasion in preeclampsia via NF-κB pathway (2022-10-31)
期刊：Reproductive Biology
DOI：10.1016/j.repbio.2022.100696
影响因子 ：2.1
引用产品： HTR-8-Svneo 细胞 , 293 [HEK-293] 细胞
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Novel Functional Recombinant Human Follicle-Stimulating Hormone Acquired from Goat Milk (2003-01-20)
期刊：Journal Of Agricultural And Food Chemistry
影响因子 ：6.1
引用产品： 293 [HEK-293] 细胞 , 293A 细胞
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BKCa channel participates in insulin‐induced lipid deposition in adipocytes by increasing intracellular calcium (2001-01-20)
期刊：Journal Of Cellular Physiology
影响因子 ：5.6
引用产品： 293 [HEK-293] 细胞
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Migration of Human Renal Tubular Epithelial Cells in Response to Physiological Electric Signals (2009-01-20)
期刊：Frontiers in Cell and Developmental Biology
影响因子 ：5.5
引用产品： HK-2 细胞 , 293 [HEK-293] 细胞
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FXII regulates the formation of deep vein thrombosis via the PI3K/AKT signaling pathway in mice (2003-01-20)
期刊：International Journal Of Molecular Medicine
影响因子 ：5.3
引用产品： 293 [HEK-293] 细胞
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Metal-bridged DNA-functionalized silica nanoparticles for multifacet biological applications (2011-01-20)
期刊：Microchemical Journal
DOI：10.1016/j.microc.2021.107017
影响因子 ：4.8
引用产品： 293 [HEK-293] 细胞
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DNA nanolantern-based split aptamer probes for in situ ATP imaging in living cells and lighting up mitochondria (2003-01-20)
期刊：Analyst
影响因子 ：4.2
引用产品： MCF7 [MCF-7] 细胞 , 293 [HEK-293] 细胞
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The pH-triggered drug release and simultaneous carrier decomposition of effervescent SiO2–drug–Na2CO3 composite nanoparticles: to improve the antitumor activity of hydrophobic drugs (2012-01-20)
期刊：RSC Advances
影响因子 ：3.9
引用产品： 293 [HEK-293] 细胞 , PUMC-HUVEC-T1
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ADAR3 alleviated inflammation and pyroptosis of neuropathic pain by targeting NLRP3 in chronic constriction injury mice (2008-01-20)
期刊：Gene
DOI：10.1016/j.gene.2021.145909
影响因子 ：3.5
引用产品： 293 [HEK-293] 细胞
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LncPVT1 promotes cartilage degradation in diabetic OA mice by downregulating miR-146a and activating TGF-β/SMAD4 signaling (2002-01-20)
期刊：Journal of Bone and Mineral Metabolism
DOI：10.1007/s00774-020-01199-7
影响因子 ：3.3
引用产品： 293 [HEK-293] 细胞
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LINC00839 Regulates Proliferation, Migration, Invasion, Apoptosis and Glycolysis in Neuroblastoma Cells Through miR-338-3p/GLUT1 Axis (2006-01-20)
期刊：Neuropsychiatric Disease and Treatment
影响因子 ：3.2
引用产品： IMR-32 细胞 , SK-N-SH 细胞 , 293 [HEK-293] 细胞 , DMEM高糖 培养基
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Circ_0116061 regulated the proliferation, apoptosis, and inflammation of osteoarthritis chondrocytes through regulating the miR-200b-3p/SMURF2 axis (2004-01-20)
期刊：Journal of Orthopaedic Surgery and Research
DOI：10.1186/s13018-021-02391-9
影响因子 ：2.6
引用产品： 293 [HEK-293] 细胞
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Long Non-Coding RNA CASC2 Overexpression Ameliorates Sepsis-Associated Acute Kidney Injury by Regulating MiR-545-3p/PPARA axis (2005-01-20)
期刊：Journal of Surgical Research
影响因子 ：2.2
引用产品： HK-2 细胞 , 293 [HEK-293] 细胞
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Rapid selection of a novel GLP-1/GIP dual receptor agonist with prolonged glycemic control and weight loss in rodent animals (2006-00-20)
期刊：Life Sciences
影响因子 ：6.1
引用产品： 293 [HEK-293] 细胞
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lncRNA DGCR 5/miR‑27a‑3p/BNIP3 promotes cell apoptosis in pancreatic cancer by regulating the p38 MAPK pathway (2006-00-20)
期刊：International Journal of Molecular Medicine
影响因子 ：5.4
引用产品： 293 [HEK-293] 细胞
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NCK1-AS1 Increases Drug Resistance of Glioma Cells to Temozolomide by Modulating miR-137/TRIM24 (2003-00-20)
期刊：CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
影响因子 ：3.4
引用产品： 293 [HEK-293] 细胞
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Human Brain–Derived Neurotrophic Factor Gene-Modified Bone Marrow Mesenchymal Stem Cells Combined With Erythropoietin Can Improve Acute Spinal Cord Injury (2003-00-20)
期刊：Dose-Response
影响因子 ：2.5
引用产品： 293 [HEK-293]
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Asparanin A from Asparagus officinalis L. Induces G0/G1 Cell Cycle Arrest and Apoptosis in Human Endometrial Carcinoma Ishikawa Cells via Mitochondrial and PI3K/AKT Signaling Pathways (2012-09-20)
期刊：Journal Of Agricultural And Food Chemistry
影响因子 ：6.1
引用产品： Ishikawa 细胞 , 293 [HEK-293] 细胞
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水稻中类受体蛋白激酶FERONIA-like Receptor 1 的表达纯化及抗体制备 (2001-09-20)
期刊：生命科学研究
DOI：10.16605/j.cnki.1007-7847.2019.05.007
影响因子 ：0.0
引用产品： 293 [HEK-293] 细胞
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Enhanced MiR-711 transcription by PPARγ induces endoplasmic reticulum stress-mediated apoptosis targeting calnexin in rat cardiomyocytes after myocardial infarction (2003-08-20)
期刊：Journal Of Molecular And Cellular Cardiology
DOI：10.1016/j.yjmcc.2018.03.006
影响因子 ：5.8
引用产品： 293 [HEK-293] 细胞
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The extracellular loop of the auxiliary β1-subunit is involved in the regulation of BKCa channel mechanosensitivity (2009-08-20)
期刊：American Journal Of Physiology-Cell Physiology
DOI：10.1152/ajpcell.00037.2018
影响因子 ：5.5
引用产品： 293 [HEK-293] 细胞
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Preparation of porous calcium phosphate microspheres with phosphate-containing molecules at room temperature for drug delivery and osteogenic differentiation (2007-08-20)
期刊：RSC Advances
影响因子 ：3.9
引用产品： 293 [HEK-293] 细胞
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ANGPTL8 negatively regulates NF-κB activation byfacilitating selective autophagic degradation ofIKKγ (2012-07-20)
期刊：Nature Communications
DOI：10.1038/s41467-017-02355-w
影响因子 ：16.6
引用产品： Hep G2 细胞 , 293 [HEK-293] 细胞 , A549 [A-549] 细胞
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