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产品概述
人主动脉内皮细胞
Cat NO.: CP-H080
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产品名称：人主动脉内皮细胞
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组织来源：主动脉组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人主动脉内皮细胞分离自主动脉组织；主动脉是体循环的动脉主干。其运行路径为：升主动脉起于左心室，至右侧第2胸肋关节高度移行为主动脉弓，弓行向左后至第4胸椎体下缘移行为降主动脉；在第12胸椎体高度穿膈的主动脉裂孔移行为腹主动脉，以上为胸主动脉，至第4腰椎体下缘分为左、右髂总动脉；髂总动脉在骶髂关节高度分为髂内、外动脉。主动脉内皮细胞是覆盖在主动脉内面的单层细胞，可分泌一系列血管活性物质而保持血管稳态，当其受到炎症或其它因素刺激后稳态被破坏而导致一些心血管疾病的发生。因此，主动脉内皮细胞已成为研究心血管疾病发病机制及治疗药物不可缺少的工具。内皮细胞或血管内皮是一薄层的专门上皮细胞，由一层扁平细胞所组成。它形成血管的内壁，是血管管腔内血液及其他血管壁(单层鳞状上皮)的接口。内皮细胞是沿着整个循环系统，由心脏直至最小的微血管。
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方法简介：
普诺赛实验室分离的人主动脉内皮细胞采用胰蛋白酶-胶原酶联合消化法结合差速贴壁法、并通过内皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人主动脉内皮细胞经CD31免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 PLL（0.1mg/ml）或明胶（0.1%） 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H080 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 内皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 人主动脉内皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Qixian granule inhibits ferroptosis in vascular endothelial cells by modulating TRPML1 in the lysosome to prevent postmenopausal atherosclerosis (2024-03-22)
作者：Meng Zhang:1.Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. ; Chenhan Mao:1.Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, China. ; Yang Dai:1.The Third Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, 210028, China. ; Xiaojin Xu:1.Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, China. ; Xindong Wang:1.Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, China. Electronic address: wangxd@njucm.edu.cn. ;
期刊：Qixian granule inhibits ferroptosis in vascular endothelial cells by modulating TRPML1 in the lysosome to prevent postmenopausal atherosclerosis
影响因子 ：5.4
引用产品： 人主动脉内皮细胞
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Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence (2023-03-10)
作者：Yinsheng Wu:1.State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, 430062, China. ; Lixu Tang:1.School of Martial Arts, Wuhan Sports University, Wuhan, Hubei, 430079, China. ; Han Huang:1.State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, 430062, China. ; Qi Yu:1.State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, 430062, China. ; Bicheng Hu:1.The Central Laboratory, Wuhan No.1 Hospital, Wuhan, Hubei, 430022, China. ; Gang Wang:1.State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, 430062, China. ; Feng Ge:1.Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, China. ; Tailang Yin:1.Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, Hubei, 4
期刊：Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence
DOI：10.1038/s41467-023-37094-8
影响因子 ：16.6
引用产品： 人主动脉内皮细胞
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Circular RNA circ_0008896 contributes to oxidized low-density lipoprotein-induced aortic endothelial cell injury via targeting miR-188-3p/NOD2 axis (2023-03-20)
作者：Liping Liu:1.Heart Function Examination Room, the Second Hospital of Dalian Medical University, Dalian, China. ; Yan Liu:1.Department of Cardiology, the Second Hospital of Dalian Medical University, Dalian, China. ; Yueyan Zhao:1.Department of Cardiology, the Second Hospital of Dalian Medical University, Dalian, China. zhaoyueyan20212021@163.com. ; 2., Dalian City, China. zhaoyueyan20212021@163.com. ;
期刊：Circular RNA circ_0008896 contributes to oxidized low-density lipoprotein-induced aortic endothelial cell injury via targeting miR-188-3p/NOD2 axis
DOI：10.1007/s12192-023-01336-x
影响因子 ：3.8
引用产品： 人主动脉内皮细胞
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miR-19a-3p affected ox-LDL-induced SDC-1/TGF-β1/Smad3 pathway in atherosclerosis (2023-03-31)
作者：Qiao Jin:1.The Changsha central Affiliated Hospital, Department of Cardiovascular Medicine, Hengyang Medical School, University of South China, Changsha, Hunan, 410004, China. 2018050779@usc.edu.cn. ; Yiming Deng:1.The Changsha central Affiliated Hospital, Department of Cardiovascular Medicine, Hengyang Medical School, University of South China, Changsha, Hunan, 410004, China. Dym1997aa@163.com. ; Liang Li:1.The Changsha central Affiliated Hospital, Department of Cardiovascular Medicine, Hengyang Medical School, University of South China, Changsha, Hunan, 410004, China. 2018050640@usc.edu.cn. ; Ran Chen:1.The Changsha central Affiliated Hospital, Department of Cardiovascular Medicine, Hengyang Medical School, University of South China, Changsha, Hunan, 410004, China. 2018050067@usc.edu.cn. ; Luping Jiang:1.The Changsha central Affiliated Hospital, Department of Cardiovascular Medicine, Hengyang Medical School, University of South China, Changsha, Hunan, 410004, China. 2018051255@us
期刊：miR-19a-3p affected ox-LDL-induced SDC-1/TGF-β1/Smad3 pathway in atherosclerosis
影响因子 ：1.6
引用产品： 人主动脉内皮细胞
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Hypoxia-induced endothelial cell-derived exosome stimulates vascular smooth muscle cell proliferation and migration (2023-11-24)
作者：Qinggen Xiong:1.Intervention Department (Vascular Surgery Department) of The Second Affiliated Hospital of Nanchang University, Nanchang, 330008, China. ; Fei Lu:1.Intervention Department (Vascular Surgery Department) of The Second Affiliated Hospital of Nanchang University, Nanchang, 330008, China. ; Xiaoming Xie:1.Intervention Department (Vascular Surgery Department) of The Second Affiliated Hospital of Nanchang University, Nanchang, 330008, China. ; Wei Zhou:1.Intervention Department (Vascular Surgery Department) of The Second Affiliated Hospital of Nanchang University, Nanchang, 330008, China. ;
期刊：Hypoxia-induced endothelial cell-derived exosome stimulates vascular smooth muscle cell proliferation and migration
影响因子 ：1.2
引用产品： 人主动脉内皮细胞 , T/G HA-VSMC 细胞
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G0S2 ameliorates oxidized low-density lipoprotein-induced vascular endothelial cell injury by regulating mitochondrial apoptosis (2022-12-01)
作者：Zenghui Liang:1.Department of Vascular Surgery, the Third Hospital of Hebei Medical University, Shijiazhuang, China. ; Wenjie Diao:1.Department of Cardiac Surgery, the First Affiliated Hospital of Bengbu Medical College, Bengbu, China. ; Yiyao Jiang:1.Department of Cardiac Surgery, the First Affiliated Hospital of Bengbu Medical College, Bengbu, China. ; Yanrong Zhang:1.Department of Vascular Surgery, the Third Hospital of Hebei Medical University, Shijiazhuang, China. ;
期刊：G0S2 ameliorates oxidized low-density lipoprotein-induced vascular endothelial cell injury by regulating mitochondrial apoptosis
影响因子 ：3.6
引用产品： 人主动脉内皮细胞 , 人主动脉内皮细胞完全培养基
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MicroRNA-217-5p ameliorates endothelial cell apoptosis induced by ox-LDL by targeting CLIC4 (2010-09-20)
作者：Xiaotian Zhang:1.Department of Neurology, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang 110004, People's Republic of China. ; Zhimin Wang:1.Department of Neurology, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang 110004, People's Republic of China. ; Weishuai Li:1.Department of Neurology, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang 110004, People's Republic of China. ; Rui Huang:1.Department of Neurology, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang 110004, People's Republic of China. ; Dongming Zheng:1.Department of Neurology, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang 110004, People's Republic of China. ; Guorong Bi:1.Department of Neurology, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang 110004, People's Republic of China. Electronic address: bigr@sj-hospital.org. ;
期刊：MicroRNA-217-5p ameliorates endothelial cell apoptosis induced by ox-LDL by targeting CLIC4
DOI：10.1016/j.numecd.2019.09.027
影响因子 ：3.9
引用产品： 人主动脉内皮细胞 , 人主动脉内皮细胞完全培养基
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