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人主动脉平滑肌细胞
Human Aortic Smooth Muscle Cells
货号:CP-H081
规格:5×10⁵Cells/T25培养瓶
价格: ¥8880
产品概述
人主动脉平滑肌细胞
Cat NO.: CP-H081
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产品名称:人主动脉平滑肌细胞
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组织来源:主动脉组织
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产品规格:5×105cells/T25细胞培养瓶
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细胞简介:
人主动脉平滑肌细胞分离自主动脉组织;主动脉是体循环的动脉主干。其运行路径为:升主动脉起于左心室,至右侧第2胸肋关节高度移行为主动脉弓,弓行向左后至第4胸椎体下缘移行为降主动脉;在第12胸椎体高度穿膈的主动脉裂孔移行为腹主动脉,以上为胸主动脉,至第4腰椎体下缘分为左、右髂总动脉;髂总动脉在骶髂关节高度分为髂内、外动脉。主动脉平滑肌细胞原代分离培养3天后,可见细胞贴壁伸展,细胞形态大小不一,呈梭形、不规则形、三角形或扇形,核卵圆形、居中;2周后细胞汇合,多数细胞伸展呈长梭形,胞浆丰富,有分枝状突起,细胞平行排列成单层或部分区域多层重叠生长,高低起伏;细胞密度低时,常交织成网状;密度高时,则排列为旋涡状或栅栏状。传代后细胞生长较快,4-6天即可汇合,并保持上述形态学特征和生长特点。主动脉平滑肌细胞在心血管疾病发生、发展中具有重要作用,以主动脉平滑肌细胞为实验研究对象,探讨心血管疾病相关发病机制是目前研究的热点;体外培养的主动脉平滑肌细胞对于研究其生理功能、药物作用以及各种致病因素作用下的病理生理改变具重要意义。
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方法简介:
普诺赛实验室分离的人主动脉平滑肌细胞采用胰蛋白酶 - 胶原酶联合消化法结合差速贴壁法制备而来,细胞总量约为5×10⁵cells/瓶。
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质量检测:
普诺赛实验室分离的人主动脉平滑肌细胞经α-SMA免疫荧光鉴定,纯度可达90%以上,且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息:
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H081 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传5代左右;3代以内状态最佳 消化液 0.25%胰蛋白酶 培养条件 气相:空气,95%;CO2,5% 人主动脉平滑肌细胞体外培养周期有限;建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养,以此保证该细胞的最佳培养状态。
产品手册
参考文献
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Targeted Delivery of Mesenchymal Stem Cell-Derived Bioinspired Exosome-Mimetic Nanovesicles with Platelet Membrane Fusion for Atherosclerotic Treatment(2024/03/13)
作者:Yu Jiang, Miao Yu, Zhifeng Song
期刊:International Journal of Nanomedicine
影响因子 :8.000
引用产品:CP-H081
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Glucagon-like peptide-1 receptor agonist exendin 4 ameliorates diabetes-associated vascular calcification by regulating mitophagy through the AMPK signaling pathway(2024/05/08)
作者:Kui Chen, Haojie Jin, Ziheng Wu
期刊:MOLECULAR MEDICINE
DOI:10.1186/s10020-024-00817-8
影响因子 :5.700
引用产品:CP-H081
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Alginate oligosaccharide alleviates vascular aging by upregulating glutathione peroxidase 7(2024/01/10)
作者:Shan Wang, Yao Yu, Jia Liu
期刊:JOURNAL OF NUTRITIONAL BIOCHEMISTRY
DOI:10.1016/j.jnutbio.2024.109578
影响因子 :5.600
引用产品:CP-H081
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Ameliorative Effect of Coenzyme Q10 on Phenotypic Transformation in Human Smooth Muscle Cells with FBN1 Knockdown(2024/02/25)
作者:Xu Zhang, Zhengyang Zhang, Sitong Wan
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 :5.600
引用产品:CP-H081
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Dynamin-related protein 1 mediates the therapeutic effect of isoliquiritigenin in diabetic intimal hyperplasia via regulation of mitochondrial fission(2024/05/15)
作者:Baofu Zhang, Ziheng Wu, Kui Chen
期刊:HYPERTENSION RESEARCH
DOI:10.1038/s41440-024-01681-z
影响因子 :5.400
引用产品:CP-H081
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GALNT3 protects against vascular calcification by reducing oxidative stress and apoptosis of smooth muscle cells(2022/12/05)
作者:Yikai Wang, Shijie Li, Lulu Zhou
期刊:EUROPEAN JOURNAL OF PHARMACOLOGY
DOI:10.1016/j.ejphar.2022.175447
影响因子 :5.000
引用产品:CP-H081
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GALNT3 protects against phosphate-induced calcification in vascular smooth muscle cells by enhancing active FGF23 and inhibiting the wnt/β-catenin signaling pathway(2022/09/23)
作者:Liwei Guo, Yikai Wang, Shijie Li
期刊:CELLULAR SIGNALLING
DOI:10.1016/j.cellsig.2022.110477
影响因子 :4.800
引用产品:CP-H081
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HIF-1α Contributes to Hypoxia-induced VSMC Proliferation and Migration by Regulating Autophagy in Type A Aortic Dissection(2023/10/02)
作者:Ben Huang, Nan Chen, Zhenhang Chen
期刊:Advanced Biology
影响因子 :3.700
引用产品:CP-H081
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Cytoprotective effects of C1s enzyme in macrophages in atherosclerosis mediated through the LRP5 and Wnt/β-catenin pathway(2024/01/12)
作者:Dong Yuan, Zhipeng Zheng, Cheng Shen
期刊:MOLECULAR IMMUNOLOGY
DOI:10.1016/j.molimm.2024.01.002
影响因子 :3.600
引用产品:CP-H081
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Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling(2021/5/9)
作者:Fang Yan, Yanyan Hao, Xinji Gong
期刊:Molecular Medicine Reports
影响因子 :3.400
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Combined effects of hyperphosphatemia and hyperglycemia on the calcification of cultured human aortic smooth muscle cells(2018/11/28)
作者:Ping Wang, Ping Zhou, Wangshan Chen
期刊:Experimental and Therapeutic Medicine
影响因子 :2.700
引用产品:CP-H081
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ADAM33 Silencing Inhibits Vascular Smooth Muscle Cell Migration and Regulates Cytokine Secretion in Airway Vascular Remodeling via the PI3K/AKT/mTOR Pathway(2022/08/31)
作者:Fang Yan, Xin Hu, Long He
期刊:Canadian Respiratory Journal
影响因子 :2.200
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Hypoxia-Inducible Factor-1α Regulates High Phosphate-Induced Vascular Calcification via Type III Sodium-Dependent Phosphate Cotransporter 1(2024/03/26)
作者:Chengkun Guo, Zhengli Quan, Jingjing Ke
期刊:Cardiology Research and Practice
影响因子 :2.100
引用产品:CP-H081
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Phosphonoformic acid reduces hyperphosphatemia-induced vascular calcification via Pit-1(2024/01/05)
作者:Hualong Zang, Yang Liu, Qiuping Teng
期刊:JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
影响因子 :1.600
引用产品:CP-H081
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