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产品概述
人主动脉平滑肌细胞
Cat NO.: CP-H081
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产品名称：人主动脉平滑肌细胞
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组织来源：主动脉组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人主动脉平滑肌细胞分离自主动脉组织；主动脉是体循环的动脉主干。其运行路径为：升主动脉起于左心室，至右侧第2胸肋关节高度移行为主动脉弓，弓行向左后至第4胸椎体下缘移行为降主动脉；在第12胸椎体高度穿膈的主动脉裂孔移行为腹主动脉，以上为胸主动脉，至第4腰椎体下缘分为左、右髂总动脉；髂总动脉在骶髂关节高度分为髂内、外动脉。主动脉平滑肌细胞原代分离培养3天后，可见细胞贴壁伸展，细胞形态大小不一，呈梭形、不规则形、三角形或扇形，核卵圆形、居中；2周后细胞汇合，多数细胞伸展呈长梭形，胞浆丰富，有分枝状突起，细胞平行排列成单层或部分区域多层重叠生长，高低起伏；细胞密度低时，常交织成网状；密度高时，则排列为旋涡状或栅栏状。传代后细胞生长较快，4-6天即可汇合，并保持上述形态学特征和生长特点。主动脉平滑肌细胞在心血管疾病发生、发展中具有重要作用，以主动脉平滑肌细胞为实验研究对象，探讨心血管疾病相关发病机制是目前研究的热点；体外培养的主动脉平滑肌细胞对于研究其生理功能、药物作用以及各种致病因素作用下的病理生理改变具重要意义。
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方法简介：
普诺赛实验室分离的人主动脉平滑肌细胞采用胰蛋白酶 - 胶原酶联合消化法结合差速贴壁法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人主动脉平滑肌细胞经α-SMA免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H081 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传5代左右；3代以内状态最佳 消化液 0.25%胰蛋白酶 人主动脉平滑肌细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Targeted Delivery of Mesenchymal Stem Cell-Derived Bioinspired Exosome-Mimetic Nanovesicles with Platelet Membrane Fusion for Atherosclerotic Treatment (2024-03-13)
作者：Yu Jiang:1.Center for Coronary Heart Disease, Department of Cardiology, Fu Wai Hospital, National Center for Cardiovascular Diseases of China, State Key Laboratory of Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China. ; Miao Yu:1.Center for Coronary Heart Disease, Department of Cardiology, Fu Wai Hospital, National Center for Cardiovascular Diseases of China, State Key Laboratory of Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China. ; Zhi-Feng Song:1.Center for Coronary Heart Disease, Department of Cardiology, Fu Wai Hospital, National Center for Cardiovascular Diseases of China, State Key Laboratory of Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China. ; Zhi-Yao Wei:1.Center for Coronary Heart Disease, Department of Cardiology, Fu Wai Hospital, N
期刊：Targeted Delivery of Mesenchymal Stem Cell-Derived Bioinspired Exosome-Mimetic Nanovesicles with Platelet Membrane Fusion for Atherosclerotic Treatment
影响因子 ：8.0
引用产品： 小鼠骨髓间充质干细胞 , 小鼠主动脉平滑肌细胞 , 人主动脉平滑肌细胞
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Alginate oligosaccharide alleviates vascular aging by upregulating glutathione peroxidase 7 (2024-01-10)
作者：Shan Wang:1.Department of Geriatric Medicine, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Yao Yu:1.Department of Geriatric Medicine, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Jia Liu:1.Department of Geriatric Medicine, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Song Hu:1.Department of Geriatric Medicine, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Shujuan Shi:1.Department of Geriatric Medicine, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Wenjing Feng:1.Department of Geriatric Medicine, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Yongjun Mao:1.Department of Geriatric Medicine, The Affiliated Hospital of Qingdao University, Qingdao, China. Electronic address: maoyongjun@qduhospital.cn. ;
期刊：Alginate oligosaccharide alleviates vascular aging by upregulating glutathione peroxidase 7
DOI：10.1016/j.jnutbio.2024.109578
影响因子 ：5.6
引用产品： 人主动脉平滑肌细胞
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Ameliorative Effect of Coenzyme Q10 on Phenotypic Transformation in Human Smooth Muscle Cells with FBN1 Knockdown (2024-02-25)
作者：Xu Zhang:1.Department of Nutrition and Health, China Agricultural University, Beijing 100193, China. ; Zhengyang Zhang:1.Department of Nutrition and Health, China Agricultural University, Beijing 100193, China. ; Sitong Wan:1.Department of Nutrition and Health, China Agricultural University, Beijing 100193, China. ; Jingyi Qi:1.Department of Nutrition and Health, China Agricultural University, Beijing 100193, China. ; Yanling Hao:1.Department of Nutrition and Health, China Agricultural University, Beijing 100193, China. ; Peng An:1.Department of Nutrition and Health, China Agricultural University, Beijing 100193, China. ; Yongting Luo:1.Department of Nutrition and Health, China Agricultural University, Beijing 100193, China. ; Junjie Luo:1.Department of Nutrition and Health, China Agricultural University, Beijing 100193, China. ;
期刊：Ameliorative Effect of Coenzyme Q10 on Phenotypic Transformation in Human Smooth Muscle Cells with FBN1 Knockdown
影响因子 ：5.6
引用产品： 人主动脉平滑肌细胞
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Cytoprotective effects of C1s enzyme in macrophages in atherosclerosis mediated through the LRP5 and Wnt/β-catenin pathway (2024-01-12)
作者：Dong Yuan:1.The Affiliated Taizhou People's Hospital of Nanjing Medical University, Taizhou, China. ; Zhipeng Zheng:1.Dalian Medical University, Dalian, China. ; Cheng Shen:1.Nanjing University of Chinese Medicine, Nanjing, China. ; Jun Ye:1.The Affiliated Taizhou People's Hospital of Nanjing Medical University, Taizhou, China. Electronic address: dingyinglinyejun@126.com. ; Li Zhu:1.The Affiliated Taizhou People's Hospital of Nanjing Medical University, Taizhou, China. Electronic address: tzheartchina@126.com. ;
期刊：Cytoprotective effects of C1s enzyme in macrophages in atherosclerosis mediated through the LRP5 and Wnt/β-catenin pathway
DOI：10.1016/j.molimm.2024.01.002
影响因子 ：3.6
引用产品： RAW 264.7 细胞 , THP-1 细胞 , H9c2 (2-1) 细胞 , HL-1 细胞 , 人主动脉平滑肌细胞 , 人脐静脉内皮细胞 , MEM 培养基 , DMEM高糖 培养基 , Ham's F-12K 培养基 , RPMI-1640 培养基 , 特级胎牛血清
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Hypoxia-Inducible Factor-1α Regulates High Phosphate-Induced Vascular Calcification via Type III Sodium-Dependent Phosphate Cotransporter 1 (2024-03-26)
作者：Chengkun Guo:1.Nephrology Department, Jingmen Central Hospital Affiliated to Hubei Minzu University, Jingmen, Hubei 448000, China. ; Zhengli Quan:1.Nephrology Department, Jingmen Central Hospital Affiliated to Hubei Minzu University, Jingmen, Hubei 448000, China. ; Jingjing Ke:1.Nephrology Department, Jingmen Central Hospital Affiliated to Hubei Minzu University, Jingmen, Hubei 448000, China. ; Hualong Zang:1.Nephrology Department, Jingmen Central Hospital Affiliated to Hubei Minzu University, Jingmen, Hubei 448000, China. ; Qiuping Teng:1.Nephrology Department, Jingmen Central Hospital Affiliated to Hubei Minzu University, Jingmen, Hubei 448000, China. ; Xin Li:1.Nephrology Department, Jingmen Central Hospital Affiliated to Hubei Minzu University, Jingmen, Hubei 448000, China. ; Dan Peng:1.Neonatology Department, Jingmen Central Hospital Affiliated to Hubei Minzu University, Jingmen, Hubei 448000, China. ; Ping Wang:1.Nephrology Department, Jingmen Central Hospital Affiliated t
期刊：Hypoxia-Inducible Factor-1α Regulates High Phosphate-Induced Vascular Calcification via Type III Sodium-Dependent Phosphate Cotransporter 1
影响因子 ：2.1
引用产品： 人主动脉平滑肌细胞
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Phosphonoformic acid reduces hyperphosphatemia-induced vascular calcification via Pit-1 (2024-01-05)
作者：Hualong Zang:1.Nephrology Department, Jingmen Central Hospital Affiliated to Hubei Minzu University, Jingmen, Hubei, China. ; 2.Nephrology Department, Jingmen Central Hospital Affiliated to Jingchu University of Technology, Jingmen, Hubei, China. ; Yang Liu:1.Nephrology Department, Jingmen Central Hospital Affiliated to Hubei Minzu University, Jingmen, Hubei, China. ; 2.Nephrology Department, Jingmen Central Hospital Affiliated to Jingchu University of Technology, Jingmen, Hubei, China. ; Qiuping Teng:1.Nephrology Department, Jingmen Central Hospital Affiliated to Hubei Minzu University, Jingmen, Hubei, China. ; 2.Nephrology Department, Jingmen Central Hospital Affiliated to Jingchu University of Technology, Jingmen, Hubei, China. ; Jiao Hua:1.Nephrology Department, Jingmen Central Hospital Affiliated to Hubei Minzu University, Jingmen, Hubei, China. ; 2.Nephrology Department, Jingmen Central Hospital Affiliated to Jingchu University of Technology, Jingmen, Hubei, China. ; Dan Peng
期刊：Phosphonoformic acid reduces hyperphosphatemia-induced vascular calcification via Pit-1
影响因子 ：1.6
引用产品： 人主动脉平滑肌细胞
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GALNT3 protects against vascular calcification by reducing oxidative stress and apoptosis of smooth muscle cells (2022-12-05)
作者：Yi-Kai Wang:1.School of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China; Xinxiang Key Laboratory of Metabolism and Integrative Physiology, Xinxiang, Henan, China. ; Shi-Jie Li:1.School of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China; Xinxiang Key Laboratory of Metabolism and Integrative Physiology, Xinxiang, Henan, China. ; Lu-Lu Zhou:1.School of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China; Xinxiang Key Laboratory of Metabolism and Integrative Physiology, Xinxiang, Henan, China. ; Duan Li:1.School of Basic Medicine, Xinxiang Medical University, Xinxiang, Henan, China. Electronic address: 061014@xxmu.edu.cn. ; Li-Wei Guo:1.School of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China; Xinxiang Key Laboratory of Metabolism and Integrative Physiology, Xinxiang, Henan, China. Electronic address: 071012@xxmu.edu.cn. ;
期刊：GALNT3 protects against vascular calcification by reducing oxidative stress and apoptosis of smooth muscle cells
DOI：10.1016/j.ejphar.2022.175447
影响因子 ：5.2
引用产品： 人主动脉平滑肌细胞
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GALNT3 protects against phosphate-induced calcification in vascular smooth muscle cells by enhancing active FGF23 and inhibiting the wnt/β-catenin signaling pathway (2022-09-23)
作者：Liwei Guo:1.School of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China; Xinxiang Key Laboratory of Metabolism and Integrative Physiology, Xinxiang, Henan, China. Electronic address: 071012@xxmu.edu.cn. ; Yikai Wang:1.School of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China; Xinxiang Key Laboratory of Metabolism and Integrative Physiology, Xinxiang, Henan, China. ; Shijie Li:1.School of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China; Xinxiang Key Laboratory of Metabolism and Integrative Physiology, Xinxiang, Henan, China. ; Lulu Zhou:1.School of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China; Xinxiang Key Laboratory of Metabolism and Integrative Physiology, Xinxiang, Henan, China. ; Duan Li:1.School of Basic Medicine, Xinxiang Medical University, Xinxiang, Henan, China. Electronic address: 061014@xxmu.edu.cn. ;
期刊：GALNT3 protects against phosphate-induced calcification in vascular smooth muscle cells by enhancing active FGF23 and inhibiting the wnt/β-catenin signaling pathway
DOI：10.1016/j.cellsig.2022.110477
影响因子 ：4.9
引用产品： 人主动脉平滑肌细胞
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ADAM33 Silencing Inhibits Vascular Smooth Muscle Cell Migration and Regulates Cytokine Secretion in Airway Vascular Remodeling via the PI3K/AKT/mTOR Pathway (2022-08-31)
作者：Fang Yan:1.School of Public Health, Xinjiang Medical University, Urumqi 830011, China. ; 2.State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Department of Respiratory Medicine, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China. ; Xin Hu:1.State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Department of Respiratory Medicine, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China. ; Long He:1.Department of Cardiology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China. ; Kegang Jiao:1.School of Public Health, Xinjiang Medical University, Urumqi 830011, China. ; Yanyan Hao:1.State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Department of Respiratory Medicine, The First Affiliated Hospital of Xinjiang Medical University, Urumqi
期刊：ADAM33 Silencing Inhibits Vascular Smooth Muscle Cell Migration and Regulates Cytokine Secretion in Airway Vascular Remodeling via the PI3K/AKT/mTOR Pathway
影响因子 ：2.1
引用产品： 人主动脉平滑肌细胞 , 人主动脉平滑肌细胞完全培养基
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Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling (2005-01-20)
作者：Fang Yan:1.School of Public Health, Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830011, P.R. China. ; Yanyan Hao:1.State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Department of Respiratory Medicine, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830054, P.R. China. ; Xinji Gong:1.State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Department of Respiratory Medicine, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830054, P.R. China. ; Hu Sun:1.State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Department of Respiratory Medicine, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830054, P.R. China. ; Jianbing Ding:1.Departmen
期刊：Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling
影响因子 ：3.4
引用产品： 人主动脉平滑肌细胞 , 人主动脉平滑肌细胞完全培养基
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Combined effects of hyperphosphatemia and hyperglycemia on the calcification of cultured human aortic smooth muscle cells (2011-08-20)
作者：Ping Wang:1.Nephrology Department, The First People's Hospital of Jingmen, Jingmen, Hubei 448000, P.R. China. ; Ping Zhou:1.Gastrointestinal Surgery Department, The Central Hospital of Hengyang, Hengyang, Hunan 421001, P.R. China. ; Wangshan Chen:1.Nephrology Department, The First People's Hospital of Jingmen, Jingmen, Hubei 448000, P.R. China. ; Dan Peng:1.Neonatology Department, The First People's Hospital of Jingmen, Jingmen, Hubei 448000, P.R. China. ;
期刊：Combined effects of hyperphosphatemia and hyperglycemia on the calcification of cultured human aortic smooth muscle cells
影响因子 ：2.7
引用产品： 人主动脉平滑肌细胞
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