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小鼠主动脉平滑肌细胞
Mouse Aortic Smooth Muscle Cells
货号：CP-M076
规格：5×10⁵Cells/T25培养瓶
价格： ￥3380
培养基货号： CM-M076
产品概述
小鼠主动脉平滑肌细胞
Cat NO.: CP-M076
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产品名称：小鼠主动脉平滑肌细胞
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组织来源：主动脉组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
小鼠主动脉平滑肌细胞分离自主动脉组织；主动脉是体循环的动脉主干。其运行路径为：升主动脉起于左心室，至右侧第2胸肋关节高度移行为主动脉弓，弓行向左后至第4胸椎体下缘移行为降主动脉；在第12胸椎体高度穿膈的主动脉裂孔移行为腹主动脉，以上为胸主动脉，至第4腰椎体下缘分为左、右髂总动脉；髂总动脉在骶髂关节高度分为髂内、外动脉。主动脉平滑肌细胞原代分离培养3天后，可见细胞贴壁伸展，细胞形态大小不一，呈梭形、不规则形、三角形或扇形，核卵圆形、居中；2周后细胞汇合，多数细胞伸展呈长梭形，胞浆丰富，有分枝状突起，细胞平行排列成单层或部分区域多层重叠生长，高低起伏；细胞密度低时，常交织成网状；密度高时，则排列为旋涡状或栅栏状。传代后细胞生长较快，4-6天即可汇合，并保持上述形态学特征和生长特点。主动脉平滑肌细胞在心血管疾病发生、发展中具有重要作用，以主动脉平滑肌细胞为实验研究对象，探讨心血管疾病相关发病机制是目前研究的热点；体外培养的主动脉平滑肌细胞对于研究其生理功能、药物作用以及各种致病因素作用下的病理生理改变具重要意义。
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方法简介：
普诺赛实验室分离的小鼠主动脉平滑肌细胞采用胰蛋白酶 - 胶原酶联合消化法结合差速贴壁法制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的小鼠主动脉平滑肌细胞经α-SMA免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-M076 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传3代左右 消化液 0.25%胰蛋白酶 小鼠主动脉平滑肌细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Early Detection of Cardiac Fibrosis in Diabetic Mice by Targeting Myocardiopathy and matrix metalloproteinase 2 (2024-01-18)
作者：Hanrui Liu:1.Key Laboratory of Obstet & Gynecol & Pediat Dis, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, China. ; Weifeng Yan:1.Department of Radiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China. ; Chengyong Ma:1.Department of Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China. ; Kun Zhang:1.Key Laboratory of Obstet & Gynecol & Pediat Dis, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, China. ; Kuan Li:1.Key Laboratory of Obstet & Gynecol & Pediat Dis, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, China. ; Rongrong Jin:1.National Engineering Research Center for Biomaterials, College of Biomedical Engineering, Sichuan University, Chengdu 610064, China. ; Huayan Xu:1.Key Laboratory of Obstet & Gynecol & Pediat Dis, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 6100
期刊：Early Detection of Cardiac Fibrosis in Diabetic Mice by Targeting Myocardiopathy and matrix metalloproteinase 2
DOI：10.1016/j.actbio.2024.01.017
影响因子 ：9.7
引用产品： 小鼠主动脉平滑肌细胞 , 小鼠主动脉内皮细胞
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Targeted Delivery of Mesenchymal Stem Cell-Derived Bioinspired Exosome-Mimetic Nanovesicles with Platelet Membrane Fusion for Atherosclerotic Treatment (2024-03-13)
作者：Yu Jiang:1.Center for Coronary Heart Disease, Department of Cardiology, Fu Wai Hospital, National Center for Cardiovascular Diseases of China, State Key Laboratory of Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China. ; Miao Yu:1.Center for Coronary Heart Disease, Department of Cardiology, Fu Wai Hospital, National Center for Cardiovascular Diseases of China, State Key Laboratory of Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China. ; Zhi-Feng Song:1.Center for Coronary Heart Disease, Department of Cardiology, Fu Wai Hospital, National Center for Cardiovascular Diseases of China, State Key Laboratory of Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China. ; Zhi-Yao Wei:1.Center for Coronary Heart Disease, Department of Cardiology, Fu Wai Hospital, N
期刊：Targeted Delivery of Mesenchymal Stem Cell-Derived Bioinspired Exosome-Mimetic Nanovesicles with Platelet Membrane Fusion for Atherosclerotic Treatment
影响因子 ：8.0
引用产品： 小鼠骨髓间充质干细胞 , 小鼠主动脉平滑肌细胞 , 人主动脉平滑肌细胞
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METTL3–METTL14 complex induces necroptosis and inflammation of vascular smooth muscle cells via promoting N6 methyladenosine mRNA methylation of receptor-interacting protein 3 in abdominal aortic aneurysms (2023-03-22)
作者：Kun Li:1.Department of Vascular and Endovascular Surgery, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, 450003, Henan, China. ; Dongbin Zhang:1.Department of Vascular and Endovascular Surgery, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, 450003, Henan, China. ; Shuiting Zhai:1.Department of Vascular and Endovascular Surgery, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, 450003, Henan, China. ; Huilin Wu:1.Department of Vascular and Endovascular Surgery, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, 450003, Henan, China. ; Hongzhi Liu:1.Heart Center of Henan Provincial People's Hospital, Central China Fuwai Hospital, Fuwai Hospital of Zhengzhou University, No. 1 Fuwai Road, Zhengdong New District, Zhengzhou, 451464, Henan, China. hong_zhi_liu@126.com. ;
期刊：METTL3–METTL14 complex induces necroptosis and inflammation of vascular smooth muscle cells via promoting N6 methyladenosine mRNA methylation of receptor-interacting protein 3 in abdominal aortic aneurysms
DOI：10.1007/s12079-023-00737-y
影响因子 ：4.1
引用产品： 小鼠主动脉平滑肌细胞 , RAW 264.7 细胞
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Arctigenin Attenuates Vascular Inflammation Induced by High Salt through TMEM16A/ESM1/VCAM-1 Pathway (2022-10-31)
作者：Mengying Zeng:1.Department of Cardiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China. ; Ziyan Xie:1.Key Laboratory of Endocrinology, Department of Endocrinology, Ministry of Health, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China. ; Jiahao Zhang:1.Department of Cardiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China. ; Shicheng Li:1.Department of Cardiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China. ; Yanxiang Wu:1.Department of Cardiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China. ; Xiaowei Yan:1.Department of Cardiology, Peking Union Medical College Hospital,
期刊：Arctigenin Attenuates Vascular Inflammation Induced by High Salt through TMEM16A/ESM1/VCAM-1 Pathway
DOI：10.3390/biomedicines10112760
影响因子 ：4.8
引用产品： 小鼠主动脉平滑肌细胞
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LncRNA SENCR suppresses abdominal aortic aneurysm formation by inhibiting smooth muscle cells apoptosis and extracellular matrix degradation (2004-01-20)
作者：Zhou Cai:1.Department of General Surgery, Xiangya Hospital Central South University, Changsha, China. ; Jianhua Huang:1.Department of General Surgery, Xiangya Hospital Central South University, Changsha, China. ; Junxiao Yang:1.Department of Orthopedics, Xiangya Hospital Central South University, Changsha, China. ; Baihong Pan:1.Department of General Surgery, Xiangya Hospital Central South University, Changsha, China. ; Wei Wang:1.Department of General Surgery, Xiangya Hospital Central South University, Changsha, China. ; Yangyang Ou:1.Department of General Surgery, Xiangya Hospital Central South University, Changsha, China. ; Xianwei Wang:1.Department of General Surgery, Xiangya Hospital Central South University, Changsha, China. ; Pu Yang:1.Department of General Surgery, Xiangya Hospital Central South University, Changsha, China. ;
期刊：LncRNA SENCR suppresses abdominal aortic aneurysm formation by inhibiting smooth muscle cells apoptosis and extracellular matrix degradation
影响因子 ：3.4
引用产品： 小鼠主动脉平滑肌细胞
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Knockdown of lncRNA PVT1 Inhibits Vascular Smooth Muscle Cell Apoptosis and Extracellular Matrix Disruption in a Murine Abdominal Aortic Aneurysm Model (2002-09-20)
作者：Zhidong Zhang:1.Department of Vascular and Endovascular Surgery, Henan Provincial People's Hospital, Henan, China. ; 2.Department of Aortic Surgery, Fuwai Central China Cardiovascular Hospital, Henan, China. ; Gangqiang Zou:1.Department of Vascular and Endovascular Surgery, Henan Provincial People's Hospital, Henan, China. ; 2.Department of Aortic Surgery, Fuwai Central China Cardiovascular Hospital, Henan, China. ; Xiaosan Chen:1.Department of Vascular and Endovascular Surgery, Henan Provincial People's Hospital, Henan, China. ; 2.Department of Aortic Surgery, Fuwai Central China Cardiovascular Hospital, Henan, China. ; Wei Lu:1.Department of Vascular and Endovascular Surgery, Henan Provincial People's Hospital, Henan, China. ; 2.Department of Aortic Surgery, Fuwai Central China Cardiovascular Hospital, Henan, China. ; Jianyang Liu:1.Department of Vascular and Endovascular Surgery, Henan Provincial People's Hospital, Henan, China. ; 2.Department of Aortic Surgery, Fuwai Central Ch
期刊：Knockdown of lncRNA PVT1 Inhibits Vascular Smooth Muscle Cell Apoptosis and Extracellular Matrix Disruption in a Murine Abdominal Aortic Aneurysm Model
DOI：10.14348/molcells.2018.0162
影响因子 ：3.8
引用产品： 小鼠主动脉平滑肌细胞
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