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产品概述
| 名称 | J774A.1 (小鼠单核巨噬细胞) (STR鉴定正确) |
| 别称 | J-774A.1; J774.A1; J774 A1; J774A.1; J 774A.1; J774 A.1 |
| 种属 | 小鼠 |
| 生长特性 | 贴壁细胞，不用胰酶消化 |
| 细胞形态 | 单核细胞、巨噬细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 1.该细胞不建议使用胰酶消化，胰酶会刺激分化；2.超过3天不传代细胞容易分化；3.培养时，存在少量分化细胞，属于正常现象。 |
| 背景描述 | J774A.1细胞来源于BABL/cN小鼠，有抗体依赖的吞噬作用，生长受硫酸葡聚糖、PPD和LPS的抑制；J774A.1细胞可产生IL-1β和大量的溶菌酶。 |
| 年龄（性别） | 雌性；成年 |
| 组织来源 | 巨噬细胞 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肉瘤细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~17 hours |
| 受体表达情况 | Complement (C3), expressed;Fc receptor, IgG, high affinity I (Fcgr1), expressed |
| 保藏机构 | ATCC; TIB-67 DSMZ; ACC-170 ECACC; 91051511 |
STR鉴定
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STR位点信息
18-3 18 6-7 12,13 5-5 14 X-1 25 1-2 17,18 7-1 24.2,25.2 8-1 13,14 1-1 16,17 19-2 14,15 15-3 22.3 12-1 16 6-4 18 4-2 21.3 3-2 14 2-1 16,17 13-1 15.2,16.2
11-2 16,17 17-2 15,16,17 -
STR鉴定图
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参考文献
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Hepatic Abnormal Secretion of Apolipoprotein C3 Promotes Inflammation in Aortic Dissection (2025-01-23)
期刊：Journal of the American Heart Association
影响因子 ：5.0
引用产品： J774A.1 细胞 , DMEM高糖 培养基
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Exploration of Molecular Mechanisms and Potential Traditional Chinese Medicine Intervention in Acute Liver Injury (2025-01-21)
期刊：Natural Product Communications
影响因子 ：1.5
引用产品： J774A.1 细胞
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An Engineered Bionic Nanoparticle Sponge as a Cytokine Trap and Reactive Oxygen Species Scavenger to Relieve Disc Degeneration and Discogenic Pain (2024-01-18)
期刊：ACS Nano
影响因子 ：17.1
引用产品： Neurobasal神经元培养基 培养基 , J774A.1 细胞
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Exosomal miRNA reprogramming in pyroptotic macrophage drives silica-induced fibroblast-to-myofibroblast transition and pulmonary fibrosis (2024-11-22)
期刊：JOURNAL OF HAZARDOUS MATERIALS
DOI：10.1016/j.jhazmat.2024.136629
影响因子 ：12.2
引用产品： NIH/3T3 细胞 , J774A.1 细胞
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Engineering Macrophage-Derived Exosome to Deliver Pirfenidone: A Novel Approach to Combat Silicotic Pulmonary Fibrosis (2024-10-09)
期刊：Advanced Healthcare Materials
影响因子 ：10.0
引用产品： J774A.1 细胞
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Acute exposure to polystyrene nanoparticles promotes liver injury by inducing mitochondrial ROS-dependent necroptosis and augmenting macrophage-hepatocyte crosstalk (2024-04-12)
期刊：Particle and Fibre Toxicology
DOI：10.1186/s12989-024-00578-6
影响因子 ：10.0
引用产品： J774A.1 细胞 , THP-1 细胞 , AML12 细胞
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Latexin deficiency limits foam cell formation and ameliorates atherosclerosis by promoting macrophage phenotype differentiation (2024-10-18)
期刊：Cell Death & Disease
DOI：10.1038/s41419-024-07141-3
影响因子 ：8.1
引用产品： J774A.1 细胞
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Xijiao Dihuang Decoction combined with Yinqiao Powder promotes autophagy-dependent ROS decrease to inhibit ROS/NLRP3/pyroptosis regulation axis in influenza virus infection (2024-02-10)
期刊：PHYTOMEDICINE
DOI：10.1016/j.phymed.2024.155446
影响因子 ：7.9
引用产品： J774A.1 细胞
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Discovery of a Covalent Inhibitor of Pro-Caspase-1 Zymogen Blocking NLRP3 Inflammasome Activation and Pyroptosis (2024-08-19)
期刊：JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1021/acs.jmedchem.4c01558
影响因子 ：6.8
引用产品： THP-1 细胞 , J774A.1 细胞 , NCTC clone 929 [L cell, L-929] 细胞
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Discovery of Covalent MLKL PROTAC Degraders via Optimization of a Theophylline Derivative Ligand for Treating Necroptosis (2024-08-24)
期刊：JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1021/acs.jmedchem.4c00949
影响因子 ：6.8
引用产品： HT-29 细胞 , U-937 细胞 , J774A.1 细胞 , NCTC clone 929 [L cell, L-929] 细胞
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Silica-induced macrophage pyroptosis propels pulmonary fibrosis through coordinated activation of relaxin and osteoclast differentiation signaling to reprogram fibroblasts (2024-02-19)
期刊：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI：10.1016/j.ecoenv.2024.116106
影响因子 ：6.8
引用产品： J774A.1 细胞 , 3T3-L1 细胞
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Discovery of novel osthole derivatives exerting anti-inflammatory effect on DSS-induced ulcerative colitis and LPS-induced acute lung injury in mice (2024-02-22)
期刊：EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1016/j.ejmech.2024.116252
影响因子 ：6.7
引用产品： J774A.1 细胞
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Exosomes derived from vMIP-II-Lamp2b gene-modified M2 cells provide neuroprotection by targeting the injured spinal cord, inhibiting chemokine signals and modulating microglia/macrophage polarization in mice (2024-04-19)
期刊：EXPERIMENTAL NEUROLOGY
DOI：10.1016/j.expneurol.2024.114784
影响因子 ：5.3
引用产品： J774A.1 细胞
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GSK3179106 ameliorates lipopolysaccharide-induced inflammation and acute lung injury by targeting P38 MAPK (2024-10-28)
期刊：RESPIRATORY RESEARCH
DOI：10.1186/s12931-024-03012-9
影响因子 ：4.7
引用产品： J774A.1 细胞
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Bif?1 inhibits activation of inflammasome through autophagy regulatory mechanism (2024-03-01)
期刊：Molecular Medicine Reports
影响因子 ：3.4
引用产品： J774A.1 细胞
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Oxidized low-density lipoprotein changes the inflammatory status and metabolomics profiles in human and mouse macrophages and microglia (2024-03-29)
期刊：Heliyon
DOI：10.1016/j.heliyon.2024.e28806
影响因子 ：3.4
引用产品： BV2 细胞 , J774A.1 细胞 , HMC3 细胞 , THP-1 细胞
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Protective effect of quercetin against macrophage-mediated hepatocyte injury via anti-inflammation, anti-apoptosis and inhibition of ferroptosis (2024-05-09)
期刊：AUTOIMMUNITY
DOI：10.1080/08916934.2024.2350202
影响因子 ：3.3
引用产品： J774A.1 细胞 , BRL 3A 细胞
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Akkermansia muciniphila alleviates abdominal aortic aneurysms via restoring CITED2 activated by EPAS1 (2024-08-29)
期刊：INFECTION AND IMMUNITY
影响因子 ：2.9
引用产品： J774A.1 细胞 , 小鼠主动脉平滑肌细胞
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Synthesis, biological evaluation and molecular dynamics simulations of new sulfonylurea derivatives bearing biphenyl moieties as potential NLRP3 inhibitors (2024-10-29)
期刊：RESEARCH ON CHEMICAL INTERMEDIATES
DOI：10.1007/s11164-024-05431-1
影响因子 ：2.8
引用产品： J774A.1 细胞 , RAW 264.7 细胞
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Design, Synthesis, in Vitro and in Vivo Biological Evaluation of Pterostilbene Derivatives for the Treatment of Inflammatory Bowel Disease (2024-08-09)
期刊：CHEMISTRY & BIODIVERSITY
影响因子 ：2.3
引用产品： HL-7702 [L-02; LO2] 细胞 , J774A.1 细胞
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Inhalable vaccine of bacterial culture supernatant extract mediates protection against fatal pulmonary anthrax (2023-03-31)
期刊：Emerging Microbes & Infections
DOI：10.1080/22221751.2023.2191741
影响因子 ：13.2
引用产品： J774A.1 细胞
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Repairing gastric ulcer with hyaluronic acid/extracellular matrix composite through promoting M2-type polarization of macrophages (2023-06-24)
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2023.125556
影响因子 ：8.2
引用产品： RAW 264.7 细胞 , J774A.1 细胞
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The mechanism of adipose mesenchymal stem cells to stabilize the immune microenvironment of pelvic floor injury by regulating pyroptosis and promoting tissue repair (2023-12-18)
期刊：Materials Today Bio
DOI：10.1016/j.mtbio.2023.100910
影响因子 ：8.2
引用产品： J774A.1 细胞 , HFL1 细胞
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Mitigation of Sepsis-Induced Acute Lung Injury by BMSC-Derived Exosomal miR-125b-5p Through STAT3-Mediated Suppression of Macrophage Pyroptosis (2023-11-28)
期刊：International Journal of Nanomedicine
影响因子 ：8.0
引用产品： J774A.1 细胞 , MEMα (不含核苷、L-谷氨酰胺) 培养基
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M1 polarization enhances the antitumor activity of chimeric antigen receptor macrophages in solid tumors (2023-03-28)
期刊：Journal of Translational Medicine
DOI：10.1186/s12967-023-04061-2
影响因子 ：7.4
引用产品： MC38 细胞 , B16-F10 细胞 , ID8 细胞 , 293T [HEK-293T] 细胞 , J774A.1 细胞
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Salmonella spvC gene suppresses macrophage/neutrophil antibacterial defense mediated by gasdermin D (2023-12-23)
期刊：INFLAMMATION RESEARCH
DOI：10.1007/s00011-023-01818-9
影响因子 ：6.7
引用产品： J774A.1 细胞
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Discovery of 4-oxo-N-phenyl-1,4-dihydroquinoline-3-carboxamide derivatives as novel anti-inflammatory agents for the treatment of acute lung injury and sepsis (2023-01-23)
期刊：EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1016/j.ejmech.2023.115144
影响因子 ：6.7
引用产品： J774A.1 细胞 , THP-1 细胞 , LX-2 细胞
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Immunoactivity of a hybrid membrane biosurface on nanoparticles: enhancing interaction with dendritic cells to augment anti-tumor immune responses (2023-12-29)
期刊：Biomaterials Science
影响因子 ：6.6
引用产品： J774A.1 细胞 , 4T1 细胞
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The CYP2E1 inhibitor Q11 ameliorates LPS-induced sepsis in mice by suppressing oxidative stress and NLRP3 activation (2023-06-07)
期刊：BIOCHEMICAL PHARMACOLOGY
影响因子 ：5.8
引用产品： RAW 264.7 细胞 , J774A.1 细胞
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Inhibition of STAT3 phosphorylation by colchicine regulates NLRP3 activation to alleviate sepsis-induced acute lung injury (2023-04-28)
期刊：INFLAMMOPHARMACOLOGY
DOI：10.1007/s10787-023-01199-9
影响因子 ：5.8
引用产品： J774A.1 细胞
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Discovery of pterostilbene analogs as novel NLRP3 inflammasome inhibitors for potential treatment of DSS-induced colitis in mice (2023-02-18)
期刊：BIOORGANIC CHEMISTRY
DOI：10.1016/j.bioorg.2023.106429
影响因子 ：5.1
引用产品： J774A.1 细胞
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The Extract of Scutellaria baicalensis Attenuates the Pattern Recognition Receptor Pathway Activation Induced by Influenza A Virus in Macrophages (2023-07-08)
期刊：Viruses-Basel
影响因子 ：4.7
引用产品： J774A.1 细胞
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Hunteriasines A – D, tryptamine-derived alkaloids from Hunteria umbellata (2023-06-15)
期刊：PHYTOCHEMISTRY
DOI：10.1016/j.phytochem.2023.113752
影响因子 ：3.8
引用产品： J774A.1 细胞
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TWEAK promotes inflammatory response in liver fibrosis (2023-07-28)
期刊：JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
影响因子 ：3.6
引用产品： 小鼠肝枯否细胞 , J774A.1 细胞
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17β-estradiol regulates adenosine triphosphate-binding cassette transporters A1 expression via estrogen receptor A to increase macrophage cholesterol efflux. (2023-10-01)
期刊：JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
影响因子 ：2.2
引用产品： J774A.1 细胞
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Precise Detection on Cell–Cell Fusion by a Facile Molecular Beacon-Based Method (2022-12-01)
期刊：ANALYTICAL CHEMISTRY
DOI：10.1021/acs.analchem.2c04852
影响因子 ：8.0
引用产品： 293 [HEK-293] 细胞 , HuH-7 细胞 , MDA-MB-231 细胞 , NCI-H1299 细胞 , J774A.1 细胞
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1,25(OH)2D3 Promotes Macrophage Efferocytosis Partly by Upregulating ASAP2 Transcription via the VDR-Bound Enhancer Region and ASAP2 May Affect Antiviral Immunity (2022-11-21)
期刊：Nutrients
影响因子 ：6.7
引用产品： THP-1 细胞 , J774A.1 细胞 , Jurkat, Clone E6-1 细胞
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Periplaneta americana extract alleviates steatohepatitis in a mouse model by modulating HMGB1-mediated inflammatory response (2022-10-03)
期刊：Frontiers in Pharmacology
影响因子 ：6.0
引用产品： NCTC 1469 细胞 , J774A.1 细胞
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Chalcone derivatives ameliorate lipopolysaccharide-induced acute lung injury and inflammation by targeting MD2 (2021-09-03)
期刊：Acta Pharmacologica Sinica
DOI：10.1038/s41401-021-00764-8
影响因子 ：8.2
引用产品： J774A.1 细胞
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Salmonella spvC Gene Inhibits Autophagy of Host Cells and Suppresses NLRP3 as Well as NLRC4 (2021-07-14)
期刊：Frontiers in Immunology
影响因子 ：7.3
引用产品： J774A.1 细胞
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Selective Expression of a SNARE-Cleaving Protease in Peripheral Sensory Neurons Attenuates Pain-Related Gene Transcription and Neuropeptide Release (2021-08-17)
期刊：International Journal Of Molecular Sciences
影响因子 ：5.6
引用产品： RAW 264.7 细胞 , J774A.1 细胞
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Salmonella spvC Gene Inhibits Pyroptosis and Intestinal Inflammation to Aggravate Systemic Infection in Mice (2020-12-15)
期刊：Frontiers in Microbiology
影响因子 ：5.2
引用产品： J774A.1 细胞
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Cell-Penetrating Peptide-Functionalized Persistent Luminescence Nanoparticles for Tracking J774A.1 Macrophages Homing to Inflamed Tissues (2019-05-13)
期刊：ACS Applied Materials & Interfaces
影响因子 ：9.5
引用产品： J774A.1 细胞 , HUV-EC-C [HUVEC]细胞专用培养基 , PUMC-HUVEC-T1
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