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产品概述
名称 | J774A.1 (小鼠单核巨噬细胞) (STR鉴定正确) |
别称 | J-774A.1; J774.A1; J774 A1; J774A.1; J 774A.1; J774 A.1 |
种属 | 小鼠 |
生长特性 | 贴壁细胞,不用胰酶消化 |
细胞形态 | 单核细胞、巨噬细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
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推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 | 1.该细胞不建议使用胰酶消化,胰酶会刺激分化;2.超过3天不传代细胞容易分化;3.培养时,存在少量分化细胞,属于正常现象。 |
背景描述 | J774A.1细胞来源于BABL/cN小鼠,有抗体依赖的吞噬作用,生长受硫酸葡聚糖、PPD和LPS的抑制;J774A.1细胞可产生IL-1β和大量的溶菌酶。 |
年龄(性别) | 雌性;成年 |
组织来源 | 巨噬细胞 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肉瘤细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~17 hours |
受体表达情况 | Complement (C3), expressed;Fc receptor, IgG, high affinity I (Fcgr1), expressed |
保藏机构 | ATCC; TIB-67 DSMZ; ACC-170 ECACC; 91051511 |
STR鉴定
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STR位点信息
18-3 18 6-7 12,13 5-5 14 X-1 25 1-2 17,18 7-1 24.2,25.2 8-1 13,14 1-1 16,17 19-2 14,15 15-3 22.3 12-1 16 6-4 18 4-2 21.3 3-2 14 2-1 16,17 13-1 15.2,16.2
11-2 16,17 17-2 15,16,17 -
STR鉴定图
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参考文献
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An Engineered Bionic Nanoparticle Sponge as a Cytokine Trap and Reactive Oxygen Species Scavenger to Relieve Disc Degeneration and Discogenic Pain (2024-01-18)
期刊:ACS Nano
影响因子 :17.1
引用产品: Neurobasal神经元培养基 培养基 , J774A.1 细胞
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Xijiao Dihuang Decoction combined with Yinqiao Powder promotes autophagy-dependent ROS decrease to inhibit ROS/NLRP3/pyroptosis regulation axis in influenza virus infection (2024-02-10)
期刊:PHYTOMEDICINE
DOI:10.1016/j.phymed.2024.155446
影响因子 :7.9
引用产品: J774A.1 细胞
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Silica-induced macrophage pyroptosis propels pulmonary fibrosis through coordinated activation of relaxin and osteoclast differentiation signaling to reprogram fibroblasts (2024-02-19)
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2024.116106
影响因子 :6.8
引用产品: J774A.1 细胞 , 3T3-L1 细胞
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Discovery of novel osthole derivatives exerting anti-inflammatory effect on DSS-induced ulcerative colitis and LPS-induced acute lung injury in mice (2024-02-22)
期刊:EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1016/j.ejmech.2024.116252
影响因子 :6.7
引用产品: J774A.1 细胞
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Bif‑1 inhibits activation of inflammasome through autophagy regulatory mechanism (2024-03-01)
期刊:Molecular Medicine Reports
影响因子 :3.4
引用产品: J774A.1 细胞
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Inhalable vaccine of bacterial culture supernatant extract mediates protection against fatal pulmonary anthrax (2023-03-31)
期刊:Emerging Microbes & Infections
DOI:10.1080/22221751.2023.2191741
影响因子 :13.2
引用产品: J774A.1 细胞
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Repairing gastric ulcer with hyaluronic acid/extracellular matrix composite through promoting M2-type polarization of macrophages (2023-06-24)
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2023.125556
影响因子 :8.2
引用产品: RAW 264.7 细胞 , J774A.1 细胞
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Mitigation of Sepsis-Induced Acute Lung Injury by BMSC-Derived Exosomal miR-125b-5p Through STAT3-Mediated Suppression of Macrophage Pyroptosis (2023-11-28)
期刊:International Journal of Nanomedicine
影响因子 :8.0
引用产品: J774A.1 细胞 , MEMα (不含核苷、L-谷氨酰胺) 培养基
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M1 polarization enhances the antitumor activity of chimeric antigen receptor macrophages in solid tumors (2023-03-28)
期刊:Journal of Translational Medicine
DOI:10.1186/s12967-023-04061-2
影响因子 :7.4
引用产品: MC38 细胞 , B16-F10 细胞 , ID8 细胞 , 293T [HEK-293T] 细胞 , J774A.1 细胞
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Salmonella spvC gene suppresses macrophage/neutrophil antibacterial defense mediated by gasdermin D (2023-12-23)
期刊:INFLAMMATION RESEARCH
DOI:10.1007/s00011-023-01818-9
影响因子 :6.7
引用产品: J774A.1 细胞
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Discovery of 4-oxo-N-phenyl-1,4-dihydroquinoline-3-carboxamide derivatives as novel anti-inflammatory agents for the treatment of acute lung injury and sepsis (2023-01-23)
期刊:EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1016/j.ejmech.2023.115144
影响因子 :6.7
引用产品: J774A.1 细胞 , THP-1 细胞 , LX-2 细胞
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Immunoactivity of a hybrid membrane biosurface on nanoparticles: enhancing interaction with dendritic cells to augment anti-tumor immune responses (2023-12-29)
期刊:Biomaterials Science
影响因子 :6.6
引用产品: J774A.1 细胞 , 4T1 细胞
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Inhibition of STAT3 phosphorylation by colchicine regulates NLRP3 activation to alleviate sepsis-induced acute lung injury (2023-04-28)
期刊:INFLAMMOPHARMACOLOGY
DOI:10.1007/s10787-023-01199-9
影响因子 :5.8
引用产品: J774A.1 细胞
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Discovery of pterostilbene analogs as novel NLRP3 inflammasome inhibitors for potential treatment of DSS-induced colitis in mice (2023-02-18)
期刊:BIOORGANIC CHEMISTRY
DOI:10.1016/j.bioorg.2023.106429
影响因子 :5.1
引用产品: J774A.1 细胞
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The Extract of Scutellaria baicalensis Attenuates the Pattern Recognition Receptor Pathway Activation Induced by Influenza A Virus in Macrophages (2023-07-08)
期刊:Viruses-Basel
影响因子 :4.7
引用产品: J774A.1 细胞
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Hunteriasines A – D, tryptamine-derived alkaloids from Hunteria umbellata (2023-06-15)
期刊:PHYTOCHEMISTRY
DOI:10.1016/j.phytochem.2023.113752
影响因子 :3.8
引用产品: J774A.1 细胞
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TWEAK promotes inflammatory response in liver fibrosis (2023-07-28)
期刊:JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
影响因子 :3.6
引用产品: 小鼠肝枯否细胞 , J774A.1 细胞
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Precise Detection on Cell–Cell Fusion by a Facile Molecular Beacon-Based Method (2022-12-01)
期刊:ANALYTICAL CHEMISTRY
DOI:10.1021/acs.analchem.2c04852
影响因子 :8.0
引用产品: 293 [HEK-293] 细胞 , HuH-7 细胞 , MDA-MB-231 细胞 , NCI-H1299 细胞 , J774A.1 细胞
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1,25(OH)2D3 Promotes Macrophage Efferocytosis Partly by Upregulating ASAP2 Transcription via the VDR-Bound Enhancer Region and ASAP2 May Affect Antiviral Immunity (2022-11-21)
期刊:Nutrients
影响因子 :6.7
引用产品: THP-1 细胞 , J774A.1 细胞 , Jurkat, Clone E6-1 细胞
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Periplaneta americana extract alleviates steatohepatitis in a mouse model by modulating HMGB1-mediated inflammatory response (2022-10-03)
期刊:Frontiers in Pharmacology
影响因子 :6.0
引用产品: NCTC 1469 细胞 , J774A.1 细胞
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Chalcone derivatives ameliorate lipopolysaccharide-induced acute lung injury and inflammation by targeting MD2 (2009-01-20)
期刊:Acta Pharmacologica Sinica
DOI:10.1038/s41401-021-00764-8
影响因子 :8.2
引用产品: J774A.1 细胞
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Salmonella spvC Gene Inhibits Autophagy of Host Cells and Suppresses NLRP3 as Well as NLRC4 (2007-01-20)
期刊:Frontiers in Immunology
影响因子 :7.3
引用产品: J774A.1 细胞
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Selective Expression of a SNARE-Cleaving Protease in Peripheral Sensory Neurons Attenuates Pain-Related Gene Transcription and Neuropeptide Release (2008-01-20)
期刊:International Journal Of Molecular Sciences
影响因子 :5.6
引用产品: RAW 264.7 细胞 , J774A.1 细胞
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Salmonella spvC Gene Inhibits Pyroptosis and Intestinal Inflammation to Aggravate Systemic Infection in Mice (2012-00-20)
期刊:Frontiers in Microbiology
影响因子 :5.2
引用产品: J774A.1 细胞
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Cell-Penetrating Peptide-Functionalized Persistent Luminescence Nanoparticles for Tracking J774A.1 Macrophages Homing to Inflamed Tissues (2005-09-20)
期刊:ACS Applied Materials & Interfaces
影响因子 :9.5
引用产品: J774A.1 细胞 , HUV-EC-C [HUVEC]细胞专用培养基 , PUMC-HUVEC-T1
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