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产品概述
G418（Geneticin，遗传霉素）是一种氨基糖苷类抗生素，在分子遗传试验中，是稳定转染最常用的抗性筛选试剂。G418（Genetici-n,遗传霉素）可以干扰80S核糖体的功能，并影响真核细胞中蛋白质的合成，主要用于哺乳动物、植物或酵母细胞的选择。G418 （Geneticin, 遗传霉素）对细菌、酵母、高等植物、哺乳动物细胞、原虫和蠕虫均有毒性，其抗性基因为显性表型，在Tn601（903）和Tn5转座子上都有定位。虽然这个基因来源于细菌，但也可以在真核细胞中表达。将抗性基因引入细胞可引起对G418的抗性，使细胞能够在含有G418的培养基中生长。G418的这一选择特性，已广泛应用于基因转移、基因敲除、抗性筛选以及转基因动物等方面。
产品信息
形态 | 液体 |
规格 | 10mL |
PH | 7.2-7.4 |
溶剂 | 100 mM HEPES |
G-418硫酸盐 | 50 mg/mL |
储存条件 | -5~-20℃，避光 |
运输条件 | 低温 |
有效期 | 24个月 |
注意事项
1、本产品经0.1 μm过滤除菌，使用时应注意无菌操作，避免污染；
2、本产品为浓缩液，请根据需要稀释后使用；
3、2-8℃中解冻，混匀后使用，切忌反复冻融，用量较少时建议分装冻存；
4、2-8℃中保存12个月内有效；
5、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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METTL3 promotes proliferation and migration of colorectal cancer cells by increasing SNHG1 stability (2023-09-26)
作者：Yeqiu Xu:1.Fourth Department of Orthopedic Surgery, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, P.R. China. ; Yuxin Bao:1.Fourth Department of Orthopedic Surgery, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, P.R. China. ; Guanzhen Qiu:1.Fourth Department of Orthopedic Surgery, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, P.R. China. ; Huinan Ye:1.Department of Medical Oncology, Cancer Hospital of Dalian University of Technology/Liaoning Cancer Hospital and Institute, Shenyang, Liaoning 110024, P.R. China. ; Ming He:1.Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110024, P.R. China. ; Xilin Wei:1.Third Department of General Surgery, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, P.R. China. ;
期刊：METTL3 promotes proliferation and migration of colorectal cancer cells by increasing SNHG1 stability
影响因子 ：3.4
引用产品： G-418溶液 (50mg/mL)
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Circ_0020014 mediates CTSB expression and participates in IL-1β-prompted chondrocyte injury via interacting with miR-24-3p (2023-11-18)
作者：Chenpeng Zhang:1.Department of Spinal Surgery, Liyang People's Hospital, Liyang, Jiangsu, China. ; Wenjun He:1.Department of Osteoarthritis, Liyang People's Hospital, Liyang, 213300, Jiangsu, China. hewenjun7282@163.com. ;
期刊：Circ_0020014 mediates CTSB expression and participates in IL-1β-prompted chondrocyte injury via interacting with miR-24-3p
DOI：10.1186/s13018-023-04370-8
影响因子 ：2.6
引用产品： G-418溶液 (50mg/mL)
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ARHGAP44-mediated regulation of the p53/C-myc/Cyclin D1 pathway in modulating the malignant biological behavior of osteosarcoma cells (2023-11-29)
作者：Shizhe Li:1.Department of Orthopedics, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China. ; 2.Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China. ; Jiancheng Xue:1.Medical Research Center, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China. ; He Zhang:1.Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China. ; Guanning Shang:1.Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China. cmushanggn@126.com. ;
期刊：ARHGAP44-mediated regulation of the p53/C-myc/Cyclin D1 pathway in modulating the malignant biological behavior of osteosarcoma cells
DOI：10.1186/s13018-023-04406-z
影响因子 ：2.6
引用产品： MG-63 细胞 , HOS 细胞 , hFOB 1.19 细胞 , MEM 培养基 , DMEM高糖 培养基 , G-418溶液 (50mg/mL)
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Identification of Prognostic RBPs in Osteosarcoma: (2003-01-20)
作者：Bei Li:1.Department of Orthopedic Oncology Surgery, Shandong Cancer Hospital, 66555Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. ; Long Fang:1.Department of Orthopaedics, Shandong Provincial Third Hospital, 66555Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. ; Baolong Wang:1.Department of Orthopaedics, Shandong Provincial Third Hospital, 66555Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. ; Zengkun Yang:1.Department of Orthopaedics, Shandong Provincial Third Hospital, 66555Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. ; Tingbao Zhao:1.Department of Orthopaedics, Shandong Provincial Third Hospital, 66555Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. ;
期刊：Identification of Prognostic RBPs in Osteosarcoma:
影响因子 ：2.8
引用产品： G-418溶液 (50mg/mL)
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