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产品概述
名称 | U-2 OS [U2OS] (人骨肉瘤细胞) (STR鉴定正确) |
别称 | U-2 OS; U-2OS; U-2-OS; U2-OS; U20-S; U20S; 2T |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | U-2 OS细胞(原名2T)是由Ponten·J和Saksela·E在1964年从一名15岁女孩的胫骨中等分化的肉瘤中分离建立的。U-2 OS细胞与WI-38细胞共培养或用抗猴空泡病毒SV40、呼吸道合胞病毒RSV或腺病毒的补体结合试验(CF法)未检测到病毒。U-2 OS细胞表达胰岛素样生长因子I、II(IGF-I、IGF-II)受体和骨肉瘤衍生生长因子(ODGF)。 |
年龄(性别) | 女性;15岁 |
组织来源 | 骨;骨肉瘤 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肉瘤细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~25-30 hours |
受体表达情况 | insulin-like growth factor I (IGF-I);insulin-like growth factor II (IGF II) |
抗原表达情况 | Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) |
基因表达情况 | osteosacoma derived growth factor (ODGF), Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) |
保藏机构 | ATCC; HTB-96 DSMZ; ACC-785 ECACC; 92022711 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 13 D2S1338 20,24 D3S1358 16 D5S818 11 D7S820 11,12 D8S1179 12,14 D13S317 13 D16S539 11,12 D18S51 14 D19S433 15 D21S11 31 FGA 20,21 PentaD 9 PentaE 10,13 TH01 6,9.3 TPOX 11,12 vWA 14,18 D6S1043 11 D12S391 19,20 D2S441 10,14 -
STR鉴定图
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参考文献
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High-content image screening to identify chemical modulators for peroxisome and ferroptosis (2024-02-17)
期刊:CELLULAR & MOLECULAR BIOLOGY LETTERS
DOI:10.1186/s11658-024-00544-2
影响因子 :8.3
引用产品: U-2 OS [U2OS] 细胞 , Hela 细胞 , McCoy's 5A 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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Roles of PTEN gene methylation in Se-CQDs induced mitochondrial apoptosis of osteosarcoma cells (2024-01-16)
期刊:COLLOIDS AND SURFACES B-BIOINTERFACES
DOI:10.1016/j.colsurfb.2024.113757
影响因子 :5.8
引用产品: 特级胎牛血清 , DMEM高糖 培养基 , U-2 OS [U2OS] 细胞
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What role does GPR65 play in the progression of osteosarcoma? Its mechanism and clinical significance (2024-01-13)
期刊:Cancer Cell International
DOI:10.1186/s12935-024-03216-5
影响因子 :5.8
引用产品: McCoy's 5A 培养基 , DMEM高糖 培养基 , U-2 OS [U2OS] 细胞 , HOS 细胞
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Soy isoflavones induces mitophagy to inhibit the progression of osteosarcoma by blocking the AKT/mTOR signaling pathway (2024-01-08)
期刊:MOLECULAR MEDICINE
DOI:10.1186/s10020-024-00778-y
影响因子 :5.7
引用产品: U-2 OS [U2OS] 细胞 , Saos-2 细胞 , MG-63 细胞
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Trifluoperazine activates AMPK / mTOR / ULK1 signaling pathway to induce mitophagy in osteosarcoma cells (2024-02-13)
期刊:CHEMICO-BIOLOGICAL INTERACTIONS
影响因子 :5.1
引用产品: 143B 细胞 , U-2 OS [U2OS] 细胞 , 人骨髓间充质干细胞
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Develop a Novel Signature to Predict the Survival and Affect the Immune Microenvironment of Osteosarcoma Patients: Anoikis-Related Genes (2024-03-27)
期刊:Journal of Immunology Research
影响因子 :4.1
引用产品: 143B 细胞 , U-2 OS [U2OS] 细胞 , hFOB 1.19 细胞 , 143B细胞专用培养基 , U-2 OS [U2OS]细胞专用培养基 , hFOB 1.19细胞专用培养基
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Circular RNA circ_0096041 promotes osteosarcoma cell proliferation and migration via sponging miR-556-5p and regulating LIN28A expression (2024-02-29)
期刊:CELLULAR AND MOLECULAR BIOLOGY
影响因子 :1.6
引用产品: MNNG/HOS Cl #5 [R-1059-D] 细胞 , U-2 OS [U2OS] 细胞 , MG-63 细胞 , hFOB 1.19 细胞
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Recruiting Rate Determines the Blinking Propensity of Rhodamine Fluorophores for Super-Resolution Imaging (2023-02-23)
期刊:Journal of the American Chemical Society
影响因子 :15.0
引用产品: Vero 细胞 , U-2 OS [U2OS] 细胞
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Bone-targeting exosome nanoparticles activate Keap1 / Nrf2 / GPX4 signaling pathway to induce ferroptosis in osteosarcoma cells (2023-09-30)
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-023-02129-1
影响因子 :10.2
引用产品: 143B 细胞 , MG-63 细胞 , Saos-2 细胞 , U-2 OS [U2OS] 细胞 , 人骨髓间充质干细胞
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Janus-Inspired Core–Shell Structure Hydrogel Programmatically Releases Melatonin for Reconstruction of Postoperative Bone Tumor (2023-01-05)
期刊:ACS Applied Materials & Interfaces
影响因子 :9.5
引用产品: U-2 OS [U2OS] 细胞 , MG-63 细胞 , B16 细胞
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Subtle Structural Translation Magically Modulates the Super-Resolution Imaging of Self-Blinking Rhodamines (2023-02-14)
期刊:ANALYTICAL CHEMISTRY
DOI:10.1021/acs.analchem.2c05298
影响因子 :7.4
引用产品: Vero 细胞 , U-2 OS [U2OS] 细胞
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A1BG-AS1 promotes the biological functions of osteosarcoma cells via regulating the microRNA-148a-3p/USP22 axis and stabilizing the expression of SIRT1 through deubiquitinase function (2023-10-03)
期刊:EXPERT OPINION ON THERAPEUTIC TARGETS
DOI:10.1080/14728222.2023.2263908
影响因子 :5.8
引用产品: U-2 OS [U2OS] 细胞 , Saos-2 细胞 , MG-63 细胞 , HOS 细胞 , SJSA-1 细胞 , hFOB 1.19 细胞 , McCoy's 5A 培养基 , MEM 培养基 , RPMI-1640 培养基 , DMEM/F12 培养基
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RILP inhibits tumor progression in osteosarcoma via Grb10-mediated inhibition of the PI3K/AKT/mTOR pathway (2023-10-03)
期刊:MOLECULAR MEDICINE
DOI:10.1186/s10020-023-00722-6
影响因子 :5.7
引用产品: 143B 细胞 , U-2 OS [U2OS] 细胞
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2-Hydroxy-3-methylanthraquinone inhibits homologous recombination repair in osteosarcoma through the MYC-CHK1-RAD51 axis (2023-01-30)
期刊:MOLECULAR MEDICINE
DOI:10.1186/s10020-023-00611-y
影响因子 :5.7
引用产品: MNNG/HOS Cl #5 [R-1059-D] 细胞 , U-2 OS [U2OS] 细胞 , MNNG/HOS Cl #5 [R-1059-D]细胞专用培养基 , U-2 OS [U2OS]细胞专用培养基
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Solasonine Inhibits Cancer Stemness and Metastasis by Modulating Glucose Metabolism via Wnt/β-Catenin/Snail Pathway in Osteosarcoma (2023-06-28)
期刊:AMERICAN JOURNAL OF CHINESE MEDICINE
影响因子 :5.7
引用产品: HOS 细胞 , U-2 OS [U2OS] 细胞
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PD-L1 Exon 3 Is a Hidden Switch of Its Expression and Function in Oral Cancer Cells (2023-05-03)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 :5.6
引用产品: FaDu 细胞 , U-2 OS [U2OS] 细胞 , Ca Ski 细胞
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m7G-related genes predict prognosis and affect the immune microenvironment and drug sensitivity in osteosarcoma (2023-08-16)
期刊:Frontiers in Pharmacology
DOI:10.3389/fphar.2023.1158775
影响因子 :5.6
引用产品: U-2 OS [U2OS] 细胞 , MG-63 细胞
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MicroRNA PC-3p-2869 Regulates Antler Growth and Inhibits Proliferation and Migration of Human Osteosarcoma and Chondrosarcoma Cells by Targeting CDK8, EEF1A1, and NTN1 (2023-06-29)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 :5.6
引用产品: 293T [HEK-293T] 细胞 , U-2 OS [U2OS] 细胞 , MG-63 细胞 , SW 1353 细胞
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ATRX loss suppresses the type I interferon response in sarcoma cells through chromatin remodeling (2023-08-15)
期刊:American Journal of Cancer Research
DOI:PMID:37693131
影响因子 :5.3
引用产品: HOS 细胞 , U-2 OS [U2OS] 细胞 , Saos-2 细胞 , 293T [HEK-293T] 细胞 , MG-63 细胞
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Circular RNA hsa_circ_001350 contributes to osteosarcoma progression by regulating microRNA-578/CCR4-NOT transcription complex and subunit 7/Wnt signaling (2023-06-15)
期刊:American Journal of Cancer Research
DOI:PMID:37424831
影响因子 :5.3
引用产品: hFOB 1.19 细胞 , U-2 OS [U2OS] 细胞 , HOS 细胞 , Saos-2 细胞 , MG-63 细胞
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Zyxin Inhibits the Proliferation, Migration, and Invasion of Osteosarcoma via Rap1-Mediated Inhibition of the MEK/ERK Signaling Pathway (2023-08-21)
期刊:Biomedicines
DOI:10.3390/biomedicines11082314
影响因子 :4.7
引用产品: 143B 细胞 , U-2 OS [U2OS] 细胞
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Sulfatinib, a novel multi-targeted tyrosine kinase inhibitor of FGFR1, CSF1R, and VEGFR1–3, suppresses osteosarcoma proliferation and invasion via dual role in tumor cells and tumor microenvironment (2023-06-08)
期刊:Frontiers in Oncology
影响因子 :4.7
引用产品: MG-63 细胞 , U-2 OS [U2OS] 细胞 , HOS 细胞 , K7M2 wt [K7M2-WT] 细胞 , RAW 264.7 细胞
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PNO1 promotes the progression of osteosarcoma via TGF-β and YAP/TAZ pathway (2023-12-09)
期刊:Scientific Reports
DOI:10.1038/s41598-023-49295-8
影响因子 :4.6
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Prognosis-related VDAC1 regulates the proliferation and apoptosis of osteosarcoma cells via the MAPK signaling pathway (2023-03-05)
期刊:GENOMICS
DOI:10.1016/j.ygeno.2023.110595
影响因子 :4.4
引用产品: hFOB 1.19 细胞 , MNNG/HOS Cl #5 [R-1059-D] 细胞 , MG-63 细胞 , 143B 细胞 , U-2 OS [U2OS] 细胞
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RING finger gene 180 inhibits osteosarcoma progression through regulating chromobox homolog 4 ubiquitination (2023-04-24)
期刊:CELL CYCLE
DOI:10.1080/15384101.2023.2205201
影响因子 :4.3
引用产品: 293T [HEK-293T] 细胞 , hFOB 1.19 细胞 , MNNG/HOS Cl #5 [R-1059-D] 细胞 , Saos-2 细胞 , MG-63 细胞 , U-2 OS [U2OS] 细胞
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Osteoblast-derived exosomes promote osteogenic differentiation of osteosarcoma cells via URG4/Wnt signaling pathway (2023-10-11)
期刊:BONE
DOI:10.1016/j.bone.2023.116933
影响因子 :4.1
引用产品: MG-63 细胞 , U-2 OS [U2OS] 细胞
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Identification of an EMT-related gene-based prognostic signature in osteosarcoma (2023-04-27)
期刊:Cancer Medicine
影响因子 :4.0
引用产品: U-2 OS [U2OS] 细胞 , MNNG/HOS Cl #5 [R-1059-D] 细胞 , McCoy's 5A 培养基 , MEM 培养基
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A short peptide LINC00665_18aa encoded by lncRNA LINC00665 suppresses the proliferation and migration of osteosarcoma cells through the regulation of the CREB1/RPS6KA3 interaction (2023-06-07)
期刊:PLoS One
DOI:10.1371/journal.pone.0286422
影响因子 :3.7
引用产品: MNNG/HOS Cl #5 [R-1059-D] 细胞 , U-2 OS [U2OS] 细胞 , DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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Disulfidptosis-related PABPC3 promotes tumor progression and inhibits immune activity in osteosarcoma (2023-12-07)
期刊:JOURNAL OF GENE MEDICINE
DOI:10.1002/jgm.3641
影响因子 :3.5
引用产品: U-2 OS [U2OS] 细胞 , MNNG/HOS Cl #5 [R-1059-D] 细胞 , McCoy's 5A 培养基 , MEM 培养基
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Diverse cell death patterns-related signature for predicting prognosis and drug sensitivity of osteosarcoma patients (2023-10-20)
期刊:JOURNAL OF GENE MEDICINE
DOI:10.1002/jgm.3613
影响因子 :3.5
引用产品: U-2 OS [U2OS] 细胞 , MNNG/HOS Cl #5 [R-1059-D] 细胞 , McCoy's 5A 培养基 , MEM 培养基
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LPS combined with CD47mAb enhances the anti‑osteosarcoma ability of macrophages (2023-03-28)
期刊:Oncology Letters
影响因子 :2.9
引用产品: RAW 264.7 细胞 , K7M2 wt [K7M2-WT] 细胞 , U-2 OS [U2OS] 细胞 , MG-63 细胞
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Hsa_circ_0000591 drives osteosarcoma glycolysis and progression by sequestering miR-194-5p and elevating HK2 expression (2023-02-21)
期刊:CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
影响因子 :2.9
引用产品: HOS 细胞 , MG-63 细胞 , U-2 OS [U2OS] 细胞 , 青霉素-链霉素溶液 (双抗),100× , hFOB 1.19 细胞
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Exosomal miR-331-3p derived from chemoresistant osteosarcoma cells induces chemoresistance through autophagy (2023-11-22)
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-023-04338-8
影响因子 :2.6
引用产品: MG-63 细胞 , HOS 细胞 , U-2 OS [U2OS] 细胞 , Saos-2 细胞
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DEPDC1 and KIF4A synergistically inhibit the malignant biological behavior of osteosarcoma cells through Hippo signaling pathway (2023-02-27)
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-023-03572-4
影响因子 :2.6
引用产品: hFOB 1.19 细胞 , U-2 OS [U2OS] 细胞 , Saos-2 细胞 , MG-63 细胞
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Piperine improves the sensitivity of osteosarcoma cells to doxorubicin by inducing apoptosis and inhibiting the PI3K/AKT/GSK-3β pathway (2023-03-09)
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-023-03642-7
影响因子 :2.6
引用产品: U-2 OS [U2OS] 细胞 , 143B 细胞
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Engineered biomimetic nanoreactor for synergistic photodynamic-chemotherapy against hypoxic tumor (2022-09-22)
期刊:JOURNAL OF CONTROLLED RELEASE
DOI:10.1016/j.jconrel.2022.09.020
影响因子 :11.5
引用产品: MNNG/HOS Cl #5 [R-1059-D] 细胞 , U-2 OS [U2OS] 细胞 , 人脐静脉内皮细胞 , RAW 264.7 细胞
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Targeting visualization of malignant tumor based on the alteration of DWI signal generated by hTERT promoter–driven AQP1 overexpression (2001-02-20)
期刊:European Journal Of Nuclear Medicine And Molecular Imaging
DOI:10.1007/s00259-022-05684-1
影响因子 :9.1
引用产品: U-2 OS [U2OS] 细胞 , 293FT 细胞 , A549 [A-549] 细胞 , Hep G2 细胞 , MDA-MB-231 细胞
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A ferroptosis-related gene signature associated with immune landscape and therapeutic response in osteosarcoma. (2022-11-11)
期刊:Frontiers in Oncology
影响因子 :5.7
引用产品: U-2 OS [U2OS] 细胞 , MNNG/HOS Cl #5 [R-1059-D] 细胞 , McCoy's 5A 培养基 , MEM 培养基
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Zinc finger Asp-His-His-Cys palmitoyl -acyltransferase 19 accelerates tumor progression through wnt/β-catenin pathway and is upregulated by miR-940 in osteosarcoma (2003-02-20)
期刊:Bioengineered
DOI:10.1080/21655979.2022.2040827
影响因子 :4.9
引用产品: U-2 OS [U2OS] 细胞 , MG-63 细胞
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Circular RNA circDOCK1 contributes to osteosarcoma progression by acting as a ceRNA for miR-936 to regulate LEF1 (2022-09-09)
期刊:Journal of Bone Oncology
影响因子 :4.5
引用产品: U-2 OS [U2OS] 细胞 , HOS 细胞 , U-2 OS [U2OS]细胞专用培养基 , HOS细胞专用培养基 , hFOB 1.19 细胞 , 青霉素-链霉素溶液 (双抗),100× , 人脐静脉内皮细胞
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PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells (2022-11-08)
期刊:Experimental and Therapeutic Medicine
影响因子 :2.8
引用产品: hFOB 1.19 细胞 , MG-63 细胞 , U-2 OS [U2OS] 细胞
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Circ_0003732 promotes osteosarcoma progression through regulating miR-377-3p/CPEB1 axis and Wnt/β-catenin signaling pathway (2001-02-20)
期刊:Anti-Cancer Drugs
DOI:10.1097/CAD.0000000000001206
影响因子 :2.4
引用产品: U-2 OS [U2OS] 细胞 , HOS 细胞 , hFOB 1.19 细胞
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Intrinsic bioactivity of black phosphorus nanomaterials on mitotic centrosome destabilization through suppression of PLK1 kinase (2008-01-20)
期刊:Nature Nanotechnology
DOI:10.1038/s41565-021-00952-x
影响因子 :38.3
引用产品: Hela 细胞 , A549 [A-549] 细胞 , MDA-MB-231 细胞 , U-2 OS [U2OS] 细胞 , Hep G2 细胞 , U-87 MG 细胞
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lncRNA MELTF-AS1 facilitates osteosarcoma metastasis by modulating MMP14 expression (2008-01-20)
期刊:Molecular Therapy-Nucleic Acids
DOI:10.1016/j.omtn.2021.08.022
影响因子 :8.8
引用产品: Saos-2 细胞 , MG-63 细胞 , U-2 OS [U2OS] 细胞 , HOS 细胞
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Down-regulation of PRR11 affects the proliferation, migration and invasion of osteosarcoma by inhibiting the Wnt/β-catenin pathway (2009-01-20)
期刊:Journal of Cancer
影响因子 :3.9
引用产品: Saos-2 细胞 , MG-63 细胞 , U-2 OS [U2OS] 细胞 , hFOB 1.19 细胞
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Schisandrin B Inhibits Cell Viability and Migration, and Induces Cell Apoptosis by circ_0009112/miR-708-5p Axis Through PI3K/AKT Pathway in Osteosarcoma (2012-01-20)
期刊:Frontiers in Genetics
影响因子 :3.7
引用产品: U-2 OS [U2OS] 细胞 , Saos-2 细胞 , hFOB 1.19 细胞
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circATP2A2 promotes osteosarcoma progression by upregulating MYH9 (2011-01-20)
期刊:Open Medicine
影响因子 :2.1
引用产品: U-2 OS [U2OS] 细胞 , HOS 细胞 , Saos-2 细胞 , 特级胎牛血清 , DMEM/F12 培养基 , hFOB 1.19 细胞
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