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产品简介
小鼠脂肪间充质干细胞成脂诱导分化培养基专门为小鼠脂肪间充质干细胞成脂诱导分化而开发，针对小鼠脂肪间充质干细胞的特性优化分化试剂的配方，可增加小鼠脂肪间充质干细胞的成脂分化效果。
本产品含血清成分，仅用于科研用途，不可用于诊断、治疗、临床及其他用途。
成脂诱导分化培养基A液：
成分名称 | 添加体积 |
成脂分化专用基础培养基A Basal Medium For Stem Cells Adipogenic Differentiation A | 177 mL |
成脂分化专用胎牛血清（FBS） Fetal Bovine Serum For Stem Cells Adipogenic Differentiation | 20 mL |
小鼠脂肪间充质干细胞成脂分化添加物A① Supplement For mADSCs Adipogenic Differentiation A① | 2.8 mL |
小鼠脂肪间充质干细胞成脂分化添加物A② Supplement For mADSCs Adipogenic Differentiation A② | 200 μL |
成脂诱导分化培养基B液：
成分名称 | 添加体积 |
成脂分化专用基础培养基B Basal Medium For Stem Cells Adipogenic Differentiation B | 177.2 mL |
成脂分化专用胎牛血清（FBS） Fetal Bovine Serum For Stem Cells Adipogenic Differentiation | 20 mL |
小鼠脂肪间充质干细胞成脂分化添加物B Supplement For mADSCs Adipogenic Differentiation B | 2.8 mL |
辅助试剂：
成分名称 | 添加体积 |
油红O染色液 Oil Red O Solution | 10 mL |
明胶包被液 Gelatin Solution | 10 mL |
注：各成分请根据试剂管上标签标示温度保存。
产品说明
| 产品规格 | 400mL |
| 储存条件 | 按标签所示温度保存，避光储存 |
| 运输条件 | 冰袋冷藏运输 |
| 有效期 | 一年 |
注意事项
1.因为培养基的成分较多，请在配制过程中严格注意无菌操作。
2. A、B交替诱导是为了减轻A液中试剂对干细胞的影响，如果您的干细胞状态较好可在前7天先只使用A液进行刺激诱导（中途每2~3天更换新鲜A液），待脂滴快速出现后，再进行两种培养基交替诱导的操作。
3.血清离心：血清中可能会存在白色絮状沉淀物，这通常是胆固醇、脂肪酸酯以及一些蛋白质析出导致，属于正常现象，该现象不影响细胞的培养和诱导分化。若您欲去除这些絮状沉淀物，可以将血清分装至无菌离心管内，以400-600 g离心5 min，取上清液使用。但是我们不建议您以过滤的方法去除这些絮状沉淀物，一方面它可能会阻塞您的过滤膜；另一方面，过滤血清这种行为可能会导致血清中部分营养成分的流失。
参考文献
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Histological study of telocytes in mice intrauterine adhesion model and their positive effect on mesenchymal stem cells in vitro (2024-02-14)
作者：Xiaojiao Wei:1.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, People's Republic of China. ; Hui Li:1.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, People's Republic of China. ; Tianquan Chen:1.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, People's Republic of China. ; 2.Department of Obstetrics and Gynecology, The Affiliated Hospital of Yangzhou University, Yangzhou City, Jiangsu Province, People's Republic of China. ; Xiaojun Yang:1.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, People's Republic of China. ;
期刊：Histological study of telocytes in mice intrauterine adhesion model and their positive effect on mesenchymal stem cells in vitro
影响因子 ：3.9
引用产品： 青霉素-链霉素溶液 (双抗)，100× , 小鼠脂肪间充质干细胞成骨诱导分化培养基 , 小鼠脂肪间充质干细胞成脂诱导分化培养基
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Exosomal circVMA21 derived from adipose-derived stem cells alleviates sepsis-induced acute kidney injury by targeting miR-16-5p (2023-07-12)
作者：Yuexian He:1.Department of PICU, The Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai, People's Republic of China. ; Xiaoyue Li:1.Department of ICU, The Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai City, People's Republic of China. ; Bolun Huang:1.Department of PICU, Guangzhou Women and Children's Medical Center, Guangzhou City, People's Republic of China. ; Yiyu Yang:1.Department of PICU, Guangzhou Women and Children's Medical Center, Guangzhou City, People's Republic of China. ; Nandu Luo:1.Zunyi Medical University, Zunyi City, People's Republic of China. ; Wenxiu Song:1.Department of PICU, The Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai, People's Republic of China. ; Bo Huang:1.Department of PICU, The Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai, People's Republic of China. ;
期刊：Exosomal circVMA21 derived from adipose-derived stem cells alleviates sepsis-induced acute kidney injury by targeting miR-16-5p
DOI：10.1097/SHK.0000000000002179
影响因子 ：3.1
引用产品： 小鼠脂肪间充质干细胞成骨诱导分化培养基 , 小鼠脂肪间充质干细胞成脂诱导分化培养基 , HK-2 细胞
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