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产品概述
产品简介
小鼠脂肪间充质干细胞成骨诱导分化培养基专门为小鼠脂肪间充质干细胞成骨诱导分化而开发，针对小鼠脂肪间充质干细胞的特性优化分化试剂的配方，可增加小鼠脂肪间充质干细胞的成骨分化效果。
本产品含血清成分，仅用于科研用途，不可用于诊断、治疗、临床及其他用途。
小鼠脂肪间充质干细胞成骨诱导分化培养基组成成分：
| 成分名称 | 添加体积 |
成骨分化专用基础培养基 Basal Medium For Stem Cells Osteogenic Differentiation | 177 mL |
成骨分化专用胎牛血清(FBS) Fetal Bovine Serum For Stem Cells Osteogenic Differentiation | 20 mL |
小鼠脂肪间充质干细胞成骨分化添加物 Supplement For mADSCs Osteogenic Differentiation | 3 mL |
染色液成分：
茜素红S染色液 Alizarin Red S | 10 mL |
明胶包被液 Gelatin Solution | 10 mL |
注：各成分请根据试剂管上标签标示温度保存。
产品说明
| 产品规格 | 200mL |
| 储存条件 | 按标签所示温度保存，避光储存 |
| 运输条件 | 冰袋冷藏运输 |
| 有效期 | 一年 |
注意事项
1. 由于成骨诱导分化实验历时较长，请在配制及使用本产品过程中严格注意无菌操作。
2. 成骨诱导实验过程中，很难通过镜下观察/白光照片判断是否已经完成诱导，而孔板一经染色又无法继续诱导。因此建议“实验伊始，在另外的孔板上多做(1-3)份平行实验，或仅将‘正常诱导组’在另外的孔板上多做(1-3)份，以便诱导后期用茜素红染色来判断正式实验的诱导进度”。
3. 小鼠脂肪间充质干细胞成骨分化添加物的颜色为白色至黄色，不影响正常使用；
4. 小鼠脂肪间充质干细胞成骨分化添加物融化后有轻微白色沉淀，该成分为L-谷氨酰胺，可将成骨分化 添加物直接添加到基础培养基中，不影响正常使用。
5. 血清离心：血清中可能会存在白色絮状沉淀物，这通常是胆固醇、脂肪酸酯以及一些蛋白质析出导致，属于正常现象，该现象不影响细胞的培养和诱导分化。若您欲去除这些絮状沉淀物，可以将血清分装至无菌离心管内，以400-600 g离心5 min，取上清液使用。但是我们不建议您以过滤的方法去除这些絮状沉淀物，一方面它可能会阻塞您的过滤膜；另一方面，过滤血清这种行为可能会导致血清中部分营养成分的流失。
参考文献
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Histological study of telocytes in mice intrauterine adhesion model and their positive effect on mesenchymal stem cells in vitro (2024-02-14)
作者：Xiaojiao Wei:1.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, People's Republic of China. ; Hui Li:1.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, People's Republic of China. ; Tianquan Chen:1.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, People's Republic of China. ; 2.Department of Obstetrics and Gynecology, The Affiliated Hospital of Yangzhou University, Yangzhou City, Jiangsu Province, People's Republic of China. ; Xiaojun Yang:1.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, People's Republic of China. ;
期刊：Histological study of telocytes in mice intrauterine adhesion model and their positive effect on mesenchymal stem cells in vitro
影响因子 ：3.9
引用产品： 青霉素-链霉素溶液 (双抗)，100× , 小鼠脂肪间充质干细胞成骨诱导分化培养基 , 小鼠脂肪间充质干细胞成脂诱导分化培养基
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Exosomal circVMA21 derived from adipose-derived stem cells alleviates sepsis-induced acute kidney injury by targeting miR-16-5p (2023-07-12)
作者：Yuexian He:1.Department of PICU, The Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai, People's Republic of China. ; Xiaoyue Li:1.Department of ICU, The Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai City, People's Republic of China. ; Bolun Huang:1.Department of PICU, Guangzhou Women and Children's Medical Center, Guangzhou City, People's Republic of China. ; Yiyu Yang:1.Department of PICU, Guangzhou Women and Children's Medical Center, Guangzhou City, People's Republic of China. ; Nandu Luo:1.Zunyi Medical University, Zunyi City, People's Republic of China. ; Wenxiu Song:1.Department of PICU, The Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai, People's Republic of China. ; Bo Huang:1.Department of PICU, The Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai, People's Republic of China. ;
期刊：Exosomal circVMA21 derived from adipose-derived stem cells alleviates sepsis-induced acute kidney injury by targeting miR-16-5p
DOI：10.1097/SHK.0000000000002179
影响因子 ：3.1
引用产品： 小鼠脂肪间充质干细胞成骨诱导分化培养基 , 小鼠脂肪间充质干细胞成脂诱导分化培养基 , HK-2 细胞
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Preparation of a cattle bone collagen peptide–calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells (2022-12-15)
作者：Hongru Zhang:1.Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. dr_zch@163.com. ; 2.Laboratory of Biomass and Green Technologies, University of Liege-Gembloux Agro-Bio Tech, Passage des Déportés 2, B-5030, Gembloux, Belgium. ; Liwei Qi:1.Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. dr_zch@163.com. ; Xiaodan Wang:1.Adaptation Physiology Group, Department of Animal Sciences, Wageningen University & Research, 6708 WD, Wageningen, The Netherlands. ; Yujie Guo:1.Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. dr_zch@163.com. ; Jiqian Liu:1.Key Laborato
期刊：Preparation of a cattle bone collagen peptide–calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells
影响因子 ：6.3
引用产品： MC3T3-E1 Subclone 24 细胞 , MEMα 培养基 , 特级胎牛血清 , 小鼠骨髓间充质干细胞成骨诱导分化培养基
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