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产品概述
名称 | MDCK [NBL-2] (犬肾细胞) (种属鉴定正确) |
别称 | MDCK (NBL-2); NBL-2; Madin-Darby Canine Kidney; Madin Darby Canine Kidney |
种属 | 狗 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
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推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | MDCK(NBL-2)细胞是由S·H·Madin和N·B·Darby在1958年9月从一只外观正常的成年雌性英国小猎犬的肾分离得到的;MDCK(NBL-2)细胞角蛋白免疫过氧化物酶染色呈阳性;MDCK(NBL-2)细胞被用于研究β-粥样蛋白前体的处理及其蛋白水解产物。 |
年龄(性别) | 雌性;成年 |
组织来源 | 肾 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
基因表达情况 | The cells are positive for keratin by immunoperoxidase staining. |
保藏机构 | ATCC; CCL-34 ATCC; CRL-6253ECACC; 84121903 ECACC; 85011435 |
种属鉴定
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种属鉴定图
参考文献
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Exploration of the protective mechanisms of Icariin against cisplatin-induced renal cell damage in canines (2024-02-21)
作者:Jiayi Liu:1.College of Police Dog Technology of Criminal Investigation Police University of China, Shenyang, China. ; Liuwei Xie:1.College of Police Dog Technology of Criminal Investigation Police University of China, Shenyang, China. ; He Zhai:1.College of Police Dog Technology of Criminal Investigation Police University of China, Shenyang, China. ; Dongwei Wang:1.College of Police Dog Technology of Criminal Investigation Police University of China, Shenyang, China. ; Xiao Li:1.Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, China. ; Yao Wang:1.College of Police Dog Technology of Criminal Investigation Police University of China, Shenyang, China. ; Mingqiang Song:1.College of Police Dog Technology of Criminal Investigation Police University of China, Shenyang, China. ; Chao Xu:1.Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, China. ;
期刊:Exploration of the protective mechanisms of Icariin against cisplatin-induced renal cell damage in canines
DOI:10.3389/fvets.2024.1331409
影响因子 :3.2
引用产品: MDBK [NBL-1] 细胞
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Endoplasmic reticulum stress contributes to pyroptosis through NF-κB/NLRP3 pathway in diabetic nephropathy (2023-04-01)
作者:Quanwei Li:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Kai Zhang:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Limin Hou:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Jianzhao Liao:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Hui Zhang:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Qingyue Han:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Jianying Guo:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Ying Li:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Lianmei
期刊:Endoplasmic reticulum stress contributes to pyroptosis through NF-κB/NLRP3 pathway in diabetic nephropathy
影响因子 :6.1
引用产品: 特级胎牛血清 , MDBK [NBL-1] 细胞
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Polydopamine-coated mesoporous silica nanoparticles co-loaded with Ziyuglycoside I and Oseltamivir for synergistic treatment of viral pneumonia (2023-09-12)
作者:Hong Wang:1.Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563000, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, Guizhou 563000, China. ; Lei Chen:1.Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563000, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, Guizhou 563000, China. ; Ruidong Li:1.Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563000, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of
期刊:Polydopamine-coated mesoporous silica nanoparticles co-loaded with Ziyuglycoside I and Oseltamivir for synergistic treatment of viral pneumonia
DOI:10.1016/j.ijpharm.2023.123412
影响因子 :5.8
引用产品: 增强型细胞活力检测试剂盒 (CCK-8) , MDBK [NBL-1] 细胞 , A549 [A-549] 细胞 , RAW 264.7 细胞
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Enhancing both oral bioavailability and anti-ischemic stroke efficacy of ginkgolide B by preparing nanocrystals self-stabilized Pickering nano-emulsion (2023-10-21)
作者:Yun Liu: 1.School of Pharmacy, Liaoning University of Traditional Chinese Medicine, No. 77, Life One Road DD port, Dalian 116600, China; Chungang Zhang: 1.School of Pharmacy, Liaoning University of Traditional Chinese Medicine, No. 77, Life One Road DD port, Dalian 116600, China;2.Department of Pharmacy, Changzhi Medical College, Changzhi, China;3.Key Laboratory of Ministry of Education, Traditional Chinese Medicine Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang, China;4.Qimeng Co., LTD, Chifeng, China; Lan Cheng: 1.School of Pharmacy, Liaoning University of Traditional Chinese Medicine, No. 77, Life One Road DD port, Dalian 116600, China; Hongxin Wang: 1.Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Jinzhou Medical University, Jinzhou, Liaoning Province, China; Meili Lu: 1.Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Jinzhou Medical University, J
期刊:Enhancing both oral bioavailability and anti-ischemic stroke efficacy of ginkgolide B by preparing nanocrystals self-stabilized Pickering nano-emulsion
DOI:10.1016/j.ejps.2023.106620
影响因子 :4.6
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Protective effect of chrysophanol on canine renal cell injury induced by canine parvovirus (2023-05-31)
作者:Ting Gong:1.Henan University of Animal Husbandry and Economy, Zhengzhou, 450000, China. gongting8301@126.com. ;
期刊:Protective effect of chrysophanol on canine renal cell injury induced by canine parvovirus
影响因子 :1.6
引用产品: MDBK [NBL-1] 细胞
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STIM1 mediates IAV-induced inflammation of lung epithelialcells by regulating NLRP3 and inflammasome activation viatargeting miR-223 (2012-00-20)
作者:Cui-Cui Liu:1.Department of Respiratory and Asthma, Xi'an Children's Hospital, Xi'an 710003, China. ; Yi Miao:1.Department of Respiratory and Critical Care Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, Shaanxi, China. ; Rui-Lin Chen:1.Department of Respiratory and Critical Care Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, Shaanxi, China. ; Yong-Qing Zhang:1.Department of Respiratory and Critical Care Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, Shaanxi, China. ; Hua Wu:1.Department of Respiratory and Critical Care Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, Shaanxi, China. ; Shu-Mei Yang:1.Department of Respiratory and Critical Care Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, Shaanxi, China. ; Li-Qun Shang:1.Department of Respiratory and Critical Care Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, Shaanxi, China. Electronic address: liqun333@foxmail.com. ;
期刊:STIM1 mediates IAV-induced inflammation of lung epithelialcells by regulating NLRP3 and inflammasome activation viatargeting miR-223
影响因子 :6.1
引用产品: BEAS-2B , MDBK [NBL-1] 细胞
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