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产品概述
名称 | L Wnt-3A (小鼠皮下结缔组织细胞) (STR鉴定正确) |
别称 | L-Wnt-3A; L-Wnt3A; LWnt3A; LWnt-3A |
种属 | 小鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:8 |
推荐换液频率 | 2-3次/周 |
背景描述 | L Wnt-3A细胞是由L-M(TK-)细胞用Wnt-3A表达载体转染并在含G418的培养基中筛选出的稳转株。Wnt-3A基因编码一个有变异的信号功效分泌性糖蛋白,Wnt基因控制胚胎发过程中的许多模式形成和生长事件。L Wnt-3A细胞分泌有生物活性的Wnt-3A蛋白,L Wnt-3A细胞是目前生产Wnt-3A条件培养基的最好来源。由于这种条件培养基还包含Wnt-3A蛋白外的其他因子,对于涉及Wnt-3A条件培养基的实验有必要用其来源细胞株作对照。 |
年龄(性别) | 雄性;100日龄 |
组织来源 | 组织:皮下结缔组织;疏松结缔组织及脂肪;品系:C3H/An |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
基因表达情况 | Wnt-3A |
保藏机构 | ATCC; CRL-2647 |
STR鉴定
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STR位点信息
18-3 15,16 6-7 12 5-5 14 X-1 26,27 1-2 17,18 7-1 25,26 8-1 16 1-1 10 19-2 12 15-3 24.3,25.3 12-1 16 6-4 17,19 4-2 20.3 3-2 14,15 2-1 9 13-1 17.1
11-2 15,16 17-2 14,15 -
STR鉴定图
参考文献
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An epitope-directed selection strategy facilitating the identification of Frizzled receptor selective antibodies (2022-12-12)
作者:Qiangqiang Ge:1.Department of Clinical Laboratory, The First Affiliated Hospital of USTC, Ministry of Education Key Laboratory for Membraneless Organelles & Cellular Dynamics, Biomedical Sciences and Health Laboratory of Anhui Province, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, 230027 Hefei, P.R. China. ; Maikun Teng:1.Ministry of Education Key Laboratory for Membraneless Organelles & Cellular Dynamics, Biomedical Sciences and Health Laboratory of Anhui Province, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, 230027 Hefei, P.R. China. Electronic address: mkteng@ustc.edu.cn. ; Xu Li:1.Ministry of Education Key Laboratory for Membraneless Organelles & Cellular Dynamics, Biomedical Sciences and Health Laboratory of Anhui Province, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, 230027 Hefei,
期刊:An epitope-directed selection strategy facilitating the identification of Frizzled receptor selective antibodies
影响因子 :5.9
引用产品: L Wnt-3A 细胞
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