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产品概述
名称 | C3H/10T1/2, Clone 8 (小鼠胚胎成纤维细胞) (STR鉴定正确) |
别称 | C3H/10T1/2-clone8; C3H/10T1/2 CL8; C3H10T1/2CL8; 10T1/2; C3H10T1-2; C3H10T1/2; C3H-10T1/2; C3H 10T1/2; C3H/10T1/2 |
种属 | 小鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | C3H/10T1/2, Clone 8细胞是由C·Reznikoff等人于1972从C3H系小鼠胚胎细胞分离。C3H/10T1/2, Clone 8细胞对过度长满的细胞有丝分裂抑制非常敏感,在同源小鼠中不产生肿瘤,无自然转化的背景;C3H/10T1/2, Clone 8细胞也不含明显内源性转化鼠类白血病或肉瘤病毒。 |
年龄(性别) | 胚胎 |
组织来源 | 胚胎 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
致瘤性 | No, in immunosuppressed mice.Yes, in semisolid medium. |
保藏机构 | ATCC; CCL-226 ECACC; 99072801 |
STR鉴定
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STR位点信息
18-3 16 6-7 12 5-5 15 X-1 26 1-2 16 7-1 26 8-1 15 1-1 10 19-2 12 15-3 25.3 12-1 16 6-4 18 4-2 20.3 3-2 14 2-1 9 13-1 17.1
11-2 16 17-2 14 -
STR鉴定图
参考文献
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Regulation of pericyte metabolic reprogramming restricts the AKI to CKD transition (2023-05-23)
作者:Cheng Xu:1.Department of Nephrology, The Second Hospital of Jilin University, Nanguan District, Changchun 130041, Jilin, China; Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Haidian District, Beijing 100853, China. ; Quan Hong:1.Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Haidian District, Beijing 100853, China. ; Kaiting Zhuang:1.Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Haidian District, Beijing 100853, China. ; Xuejing Ren:1.Departm
期刊:Regulation of pericyte metabolic reprogramming restricts the AKI to CKD transition
DOI:10.1016/j.metabol.2023.155592
影响因子 :9.8
引用产品: C3H/10T1/2, Clone 8 细胞
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Prussian Blue Nanoparticle-Entrapped GelMA Gels Laden with Mesenchymal Stem Cells as Prospective Biomaterials for Pelvic Floor Tissue Repair (2023-01-31)
作者:Jirui Wen:1.Department of Obstetrics and Gynecology, Key Laboratory of Birth Defects and Related Diseases of Women and Children of MOE, West China Second University Hospital, Sichuan University, Chengdu 610041, China. ; 2.Deep Underground Space Medical Center, West China Hospital, Sichuan University, Chengdu 610041, China. ; Zhiwei Zhao:1.West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China. ; Fei Fang:1.West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China. ; Jingyue Xiao:1.West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China. ; Ling Wang:1.Deep Underground Space Medical Center, West China Hospital, Sichuan University, Chengdu 610041, China. ; Juan Cheng:1.Deep Underground Space Medical Center, West China Hospital, Sichuan University, Chengdu 610041, China. ; Jiang Wu:1.Deep Underground Space Medical Center, West China H
期刊:Prussian Blue Nanoparticle-Entrapped GelMA Gels Laden with Mesenchymal Stem Cells as Prospective Biomaterials for Pelvic Floor Tissue Repair
影响因子 :5.6
引用产品: C3H/10T1/2, Clone 8 细胞
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The histone methyltransferase DOT1L is a new epigenetic regulator of pulmonary fibrosis (2001-02-20)
作者:Di Yang:1.Pharmacophenomics Laboratory, Human Phenome Institute, Fudan University, 201203, Shanghai, China. ; Peng Xu:1.Pharmacophenomics Laboratory, Human Phenome Institute, Fudan University, 201203, Shanghai, China. ; 2.Department of Pharmacy, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China. ; Haibi Su:1.Pharmacophenomics Laboratory, Human Phenome Institute, Fudan University, 201203, Shanghai, China. ; Wen Zhong:1.Pharmacophenomics Laboratory, Human Phenome Institute, Fudan University, 201203, Shanghai, China. ; Jie Xu:1.Pharmacophenomics Laboratory, Human Phenome Institute, Fudan University, 201203, Shanghai, China. ; Zhenghua Su:1.Pharmacophenomics Laboratory, Human Phenome Institute, Fudan University, 201203, Shanghai, China. ; Xinhua Liu:1.Pharmacophenomics Laboratory, Human Phenome Institute, Fudan University, 201203, Shanghai, China. liuxinhua@fudan.edu.cn. ;
期刊:The histone methyltransferase DOT1L is a new epigenetic regulator of pulmonary fibrosis
DOI:10.1038/s41419-021-04365-5
影响因子 :9.0
引用产品: C3H/10T1/2, Clone 8 细胞
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Olanzapine induces weight gain in offspring of prenatally exposed poly I:C rats by reducing brown fat thermogenic activity (2022-09-30)
作者:Xiaoying Chen:1.College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China. ; 2.The Second Affiliated Hospital, North Sichuan Medical College, Nanchong, Sichuan, China. ; Lu Liu:1.College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China. ; 2.Department of Preclinical Medicine, North Sichuan Medical College, Nanchong, Sichuan, China. ; Yanping Zeng:1.College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China. ; Dejuan Li:1.College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China. ; Xuemei Liu:1.College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China. ; Changhua Hu:1.College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China. ;
期刊:Olanzapine induces weight gain in offspring of prenatally exposed poly I:C rats by reducing brown fat thermogenic activity
DOI:10.3389/fphar.2022.1001919
影响因子 :6.0
引用产品: C3H/10T1/2, Clone 8 细胞
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A Quartet Network Analysis Identifying Mechanically Responsive Long Noncoding RNAs in Bone Remodeling (2003-02-20)
作者:Jingyi Cai:1.State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. ; Chaoyuan Li:1.Department of Oral Implantology, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, School and Hospital of Stomatology, Tongji University, Shanghai, China. ; Shun Li:1.Institute of Engineering Medicine, Beijing Institute of Technology, Beijing, China. ; Jianru Yi:1.State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. ; Jun Wang:1.State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. ; Ke Yao:1.State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University,
期刊:A Quartet Network Analysis Identifying Mechanically Responsive Long Noncoding RNAs in Bone Remodeling
影响因子 :5.7
引用产品: C3H/10T1/2, Clone 8 细胞
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Role of Yes-associated protein (YAP) in regulation of mesenchymal stem cell tenogenic differentiation (2001-02-20)
作者:Ying Li:1.Department of Clinical Laboratory, Second Affiliated Hospital of Dalian Medical University, Dalian, 116023, China. ; Chenyang Xiao:1.Department of Orthopaedics Surgery, First Affiliated Hospital of Dalian Medical University, 222 Zhongshan Road, Dalian, 116011, China. ; 2.Department of Clinical Laboratory, Second Affiliated Hospital of Dalian Medical University, Dalian, 116023, China. ; Ruihua Li:1.Department of Clinical Laboratory, Second Affiliated Hospital of Dalian Medical University, Dalian, 116023, China. ; Weiliang Zhong:1.Department of Orthopaedics Surgery, First Affiliated Hospital of Dalian Medical University, 222 Zhongshan Road, Dalian, 116011, China. zwl8632767@163.com. ; 2.Key Laboratory of Molecular Mechanism for Repair and Remodeling of Orthopaedic Diseases, Liaoning, 116011, China. zwl8632767@163.com. ; Gang Xu:1.Key Laboratory of Molecular Mechanism for Repair and Remodeling of Orthopaedic Diseases, Liaoning, 116011, China. ; Weiguo Zhang:1.Department of
期刊:Role of Yes-associated protein (YAP) in regulation of mesenchymal stem cell tenogenic differentiation
DOI:10.1007/s10735-022-10059-9
影响因子 :3.2
引用产品: C3H/10T1/2, Clone 8 细胞
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Stem Cell-Recruiting Injectable Microgels for Repairing Osteoarthritis (2007-01-20)
作者:Yiting Lei: 1.Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing, 400016 P. R. China; Yuping Wang: 1.Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing, 400016 P. R. China; Jieliang Shen: 1.Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing, 400016 P. R. China; Zhengwei Cai: 1.Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025 P. R. China; Yongsheng Zeng: 1.Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing, 400016 P. R. China; Piao Zhao: 1
期刊:Stem Cell-Recruiting Injectable Microgels for Repairing Osteoarthritis
影响因子 :19.0
引用产品: C3H/10T1/2, Clone 8 细胞
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miR-199b-5p promoted chondrogenic differentiation of C3H10T1/2 cells by regulating JAG1 (2012-00-20)
作者:Miao Zhang:1.Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. ; Shu Zheng Yuan:1.Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. ; Haimei Sun:1.Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. ; Lei Sun:1.Musculosketetal Tissue Bank, Beijing Jishuitan Hospital, Beijing, China. ; Deshan Zhou:1.Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. ; Jihong Yan:1.Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. ;
期刊:miR-199b-5p promoted chondrogenic differentiation of C3H10T1/2 cells by regulating JAG1
影响因子 :3.3
引用产品: C3H/10T1/2, Clone 8 细胞 , MEM 培养基
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miR‐199b‐5p promoted chondrogenic differentiation of C3H10T1/2 cells by regulating JAG1 (2009-00-20)
作者:Miao Zhang:1.Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. ; Shu Zheng Yuan:1.Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. ; Haimei Sun:1.Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. ; Lei Sun:1.Musculosketetal Tissue Bank, Beijing Jishuitan Hospital, Beijing, China. ; Deshan Zhou:1.Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. ; Jihong Yan:1.Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, China. ;
期刊:miR‐199b‐5p promoted chondrogenic differentiation of C3H10T1/2 cells by regulating JAG1
影响因子 :3.3
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