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产品概述
| 名称 | C3H/10T1/2, Clone 8 (小鼠胚胎成纤维细胞) (STR鉴定正确) |
| 别称 | C3H/10T1/2-clone8; C3H/10T1/2 CL8; C3H10T1/2CL8; 10T1/2; C3H10T1-2; C3H10T1/2; C3H-10T1/2; C3H 10T1/2; C3H/10T1/2 |
| 种属 | 小鼠 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | C3H/10T1/2, Clone 8细胞是由C·Reznikoff等人于1972从C3H系小鼠胚胎细胞分离。C3H/10T1/2, Clone 8细胞对过度长满的细胞有丝分裂抑制非常敏感，在同源小鼠中不产生肿瘤，无自然转化的背景；C3H/10T1/2, Clone 8细胞也不含明显内源性转化鼠类白血病或肉瘤病毒。 |
| 年龄（性别） | 胚胎 |
| 组织来源 | 胚胎 |
| 细胞类型 | 自发永生化细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | No, in immunosuppressed mice.Yes, in semisolid medium. |
| 保藏机构 | ATCC; CCL-226 ECACC; 99072801 |
STR鉴定
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STR位点信息
18-3 16 6-7 12 5-5 15 X-1 26 1-2 16 7-1 26 8-1 15 1-1 10 19-2 12 15-3 25.3 12-1 16 6-4 18 4-2 20.3 3-2 14 2-1 9 13-1 17.1
11-2 16 17-2 14 -
STR鉴定图
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参考文献
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Regulation of pericyte metabolic reprogramming restricts the AKI to CKD transition (2023-05-23)
期刊：METABOLISM-CLINICAL AND EXPERIMENTAL
DOI：10.1016/j.metabol.2023.155592
影响因子 ：9.8
引用产品： C3H/10T1/2, Clone 8 细胞
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Prussian Blue Nanoparticle-Entrapped GelMA Gels Laden with Mesenchymal Stem Cells as Prospective Biomaterials for Pelvic Floor Tissue Repair (2023-01-31)
期刊：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 ：5.6
引用产品： C3H/10T1/2, Clone 8 细胞
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The histone methyltransferase DOT1L is a new epigenetic regulator of pulmonary fibrosis (2001-02-20)
期刊：Cell Death & Disease
DOI：10.1038/s41419-021-04365-5
影响因子 ：9.0
引用产品： C3H/10T1/2, Clone 8 细胞
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Olanzapine induces weight gain in offspring of prenatally exposed poly I:C rats by reducing brown fat thermogenic activity (2022-09-30)
期刊：Frontiers in Pharmacology
DOI：10.3389/fphar.2022.1001919
影响因子 ：6.0
引用产品： C3H/10T1/2, Clone 8 细胞
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A Quartet Network Analysis Identifying Mechanically Responsive Long Noncoding RNAs in Bone Remodeling (2003-02-20)
期刊：Frontiers in Bioengineering and Biotechnology
影响因子 ：5.7
引用产品： C3H/10T1/2, Clone 8 细胞
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Role of Yes-associated protein (YAP) in regulation of mesenchymal stem cell tenogenic differentiation (2001-02-20)
期刊：Journal Of Molecular Histology
DOI：10.1007/s10735-022-10059-9
影响因子 ：3.2
引用产品： C3H/10T1/2, Clone 8 细胞
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Stem Cell-Recruiting Injectable Microgels for Repairing Osteoarthritis (2007-01-20)
期刊：Advanced Functional Materials
影响因子 ：19.0
引用产品： C3H/10T1/2, Clone 8 细胞
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miR-199b-5p promoted chondrogenic differentiation of C3H10T1/2 cells by regulating JAG1 (2012-00-20)
期刊：Journal of Tissue Engineering and Regenerative Medicine
影响因子 ：3.3
引用产品： C3H/10T1/2, Clone 8 细胞 , MEM 培养基
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miR‐199b‐5p promoted chondrogenic differentiation of C3H10T1/2 cells by regulating JAG1 (2009-00-20)
期刊：Journal of Tissue Engineering and Regenerative Medicine
影响因子 ：3.3
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