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产品概述
名称 | NCTC 1469 (小鼠正常肝细胞) (STR鉴定正确) |
别称 | NCTC clone 1469; NCTC1469 |
种属 | 小鼠 |
生长特性 | 半贴半悬 |
细胞形态 | 圆形、上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
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推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞生长状态为半贴半悬,贴壁部分处理时需要用胰酶消化。培养时,间隔处理时间过长会导致细胞贴壁部分消化时间延长。 |
背景描述 | NCTC 1469细胞是于1952年建系,源于正常C3H/An小鼠的肝脏组织;NCTC 1469细胞可表达H-2K抗原,鼠痘病毒阴性。 |
年龄(性别) | 新生 |
组织来源 | 肝脏 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
保藏机构 | ATCC; CCL-9.1 ECACC; 88111403 |
STR鉴定
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STR位点信息
18-3 16 6-7 12 5-5 15 X-1 26 1-2 16 7-1 26,27 8-1 14,16 1-1 10 19-2 11,12 15-3 25.3 12-1 16 6-4 18 4-2 20.3 3-2 14,15 2-1 9 13-1 17.1,18.1
11-2 16,18 17-2 15,16,17 -
STR鉴定图
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参考文献
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Reprogramming Exosomes to Escape from Immune Surveillance for Mitochondrial Protection in Hepatic Ischemia-Reperfusion Injury (2024-01-01)
期刊:Theranostics
影响因子 :12.4
引用产品: NCTC 1469 细胞 , THP-1 细胞 , 马血清
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Natural long-chain saturated fatty acids doped LNPs enabling spleen selective mRNA translation and potent cancer immunotherapy (2023-09-08)
期刊:Nano Research
影响因子 :9.9
引用产品: NCTC 1469 细胞
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Perfluorooctane sulfonate (PFOS), a novel environmental pollutant, induces liver injury in mice by activating hepatocyte ferroptosis (2023-10-27)
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2023.115625
影响因子 :6.8
引用产品: AML12 细胞 , AML-12细胞专用培养基
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Resmetirom Ameliorates NASH-Model Mice by Suppressing STAT3 and NF-κB Signaling Pathways in an RGS5-Dependent Manner (2023-03-19)
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 :5.6
引用产品: NCTC 1469 细胞
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Uncarboxylated Osteocalcin Decreases SCD1 by Activating AMPK to Alleviate Hepatocyte Lipid Accumulation (2023-03-31)
期刊:MOLECULES
影响因子 :4.6
引用产品: NCTC 1469 细胞 , DMEM高糖 培养基 , 马血清
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The Different Mechanisms of Lipid Accumulation in Hepatocytes Induced by Oleic Acid/Palmitic Acid and High-Fat Diet (2023-09-20)
期刊:MOLECULES
影响因子 :4.6
引用产品: NCTC 1469 细胞 , DMEM高糖 培养基 , 马血清
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Effects of protoscoleces excretory-secretory products of Echinococcus granulosus on hepatocyte growth, function, and glucose metabolism (2023-11-08)
期刊:ACTA TROPICA
DOI:10.1016/j.actatropica.2023.107066
影响因子 :2.7
引用产品: NCTC 1469 细胞
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Dietary intake of fructose increases purine de novo synthesis: A crucial mechanism for hyperuricemia (2022-12-19)
期刊:Frontiers in Nutrition
影响因子 :6.6
引用产品: NCTC 1469 细胞
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Periplaneta americana extract alleviates steatohepatitis in a mouse model by modulating HMGB1-mediated inflammatory response (2022-10-03)
期刊:Frontiers in Pharmacology
影响因子 :6.0
引用产品: NCTC 1469 细胞 , J774A.1 细胞
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RNA-seq transcriptome profiling of liver regeneration in mice identifies the miR-34b-5p/phosphoinositide-dependent protein kinase 1 axis as a potential target for hepatocyte proliferation (2022-08-21)
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2022.08.049
影响因子 :3.3
引用产品: NCTC 1469 细胞
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Inhibition of GCN2 alleviates hepatic steatosis and oxidative stress in obese mice: Involvement of NRF2 regulation (2012-01-20)
期刊:Redox Biology
DOI:10.1016/j.redox.2021.102224
影响因子 :11.4
引用产品: NCTC 1469 细胞
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Downregulated long non-coding RNA LINC01093 in liver fibrosis promotes hepatocyte apoptosis via increasing ubiquitination of SIRT1 (2005-00-20)
期刊:Journal Of Biochemistry
影响因子 :2.7
引用产品: NCTC 1469 细胞
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