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产品概述
名称 | NCTC 1469 (小鼠正常肝细胞) (STR鉴定正确) |
别称 | NCTC clone 1469; NCTC1469 |
种属 | 小鼠 |
生长特性 | 半贴半悬 |
细胞形态 | 圆形、上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞生长状态为半贴半悬,贴壁部分处理时需要用胰酶消化。培养时,间隔处理时间过长会导致细胞贴壁部分消化时间延长。 |
背景描述 | NCTC 1469细胞是于1952年建系,源于正常C3H/An小鼠的肝脏组织;NCTC 1469细胞可表达H-2K抗原,鼠痘病毒阴性。 |
年龄(性别) | 新生 |
组织来源 | 肝脏 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
保藏机构 | ATCC; CCL-9.1 ECACC; 88111403 |
STR鉴定
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STR位点信息
18-3 16 6-7 12 5-5 15 X-1 26 1-2 16 7-1 26,27 8-1 14,16 1-1 10 19-2 11,12 15-3 25.3 12-1 16 6-4 18 4-2 20.3 3-2 14,15 2-1 9 13-1 17.1,18.1
11-2 16,18 17-2 15,16,17 -
STR鉴定图
参考文献
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Reprogramming Exosomes to Escape from Immune Surveillance for Mitochondrial Protection in Hepatic Ischemia-Reperfusion Injury (2024-01-01)
作者:Shanshan Liu:1.School of Basic Medical Science, Chongqing Medical University, Chongqing 400016, P. R. China. ; 2.Department of Plastic and Maxillofacial Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P. R. China. ; Xinyu Xiao:1.School of Basic Medical Science, Chongqing Medical University, Chongqing 400016, P. R. China. ; La Zhang:1.Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P. R. China. ; Jianwei Wang:1.School of Basic Medical Science, Chongqing Medical University, Chongqing 400016, P. R. China. ; Wei Zhao:1.School of Basic Medical Science, Chongqing Medical University, Chongqing 400016, P. R. China. ; Haichuan Liu:1.Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P. R. China. ; Rui Liao:1.Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chong
期刊:Reprogramming Exosomes to Escape from Immune Surveillance for Mitochondrial Protection in Hepatic Ischemia-Reperfusion Injury
影响因子 :12.4
引用产品: NCTC 1469 细胞 , THP-1 细胞 , 马血清
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Natural long-chain saturated fatty acids doped LNPs enabling spleen selective mRNA translation and potent cancer immunotherapy (2023-09-08)
作者:Fazhan Wang: 1.Medical Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, 450052, China; Meng Zhang: 1.Medical Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, 450052, China; Jia Lou: 1.Medical Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, 450052, China; Longze Pan: 1.Medical Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, 450052, China; **aoke Gao: 1.Medical Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, 450052, China; Li**g Zhang: 1.Medical Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, 450052, China; **aohan Lou: 1.Medical Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, 450052, China; Linyu
期刊:Natural long-chain saturated fatty acids doped LNPs enabling spleen selective mRNA translation and potent cancer immunotherapy
影响因子 :9.9
引用产品: NCTC 1469 细胞
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Perfluorooctane sulfonate (PFOS), a novel environmental pollutant, induces liver injury in mice by activating hepatocyte ferroptosis (2023-10-27)
作者:Yang Yang: 1.Shenyang Medical College, China; Liuwei Xie: 1.Criminal Investigation Police University of China, China; Yu Zhu: 1.The Second Affiliated Hospital of Jiaxing University, China; Yongjia Sheng: 1.The Second Affiliated Hospital of Jiaxing University, China; Jin Wang: 1.The Second Affiliated Hospital of Jiaxing University, China; Xiaohong Zhou: 1.The Second Affiliated Hospital of Jiaxing University, China; Wenyan Li: 1.The Second Affiliated Hospital of Jiaxing University, China; Chenxi Cao: 1.The Second Affiliated Hospital of Jiaxing University, China; Yi Yang: 1.The Second Affiliated Hospital of Jiaxing University, China; Chenyang Han: 1.Department of pharmacy, The Second Affiliated Hospital of Jiaxing University, 314001, China;
期刊:Perfluorooctane sulfonate (PFOS), a novel environmental pollutant, induces liver injury in mice by activating hepatocyte ferroptosis
DOI:10.1016/j.ecoenv.2023.115625
影响因子 :6.8
引用产品: AML12 细胞 , AML-12细胞专用培养基
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Resmetirom Ameliorates NASH-Model Mice by Suppressing STAT3 and NF-κB Signaling Pathways in an RGS5-Dependent Manner (2023-03-19)
作者:Xiaojing Wang:1.Department of Gastroenterology, Lishui Hospital of Zhejiang University/The Central Hospital of Lishui, Lishui 323000, China. ; Liangjing Wang:1.Department of Gastroenterology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China. ; 2.Institute of Gastroenterology, Zhejiang University, Hangzhou 310009, China. ; Lin Geng:1.Department of Gastroenterology, Lishui Hospital of Zhejiang University/The Central Hospital of Lishui, Lishui 323000, China. ; Naoki Tanaka:1.Department of Global Medical Research Promotion, Shinshu University Graduate School of Medicine, Matsumoto 390-8621, Japan. ; 2.International Relations Office, Shinshu University School of Medicine, Matsumoto 390-8621, Japan. ; 3.Research Center for Social Systems, Shinshu University, Matsumoto 390-8621, Japan. ; Bin Ye:1.Department of Gastroenterology, Lishui Hospital of Zhejiang University/The Central Hospital of Lishui, Lishui 323000, China. ;
期刊:Resmetirom Ameliorates NASH-Model Mice by Suppressing STAT3 and NF-κB Signaling Pathways in an RGS5-Dependent Manner
影响因子 :5.6
引用产品: NCTC 1469 细胞
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Uncarboxylated Osteocalcin Decreases SCD1 by Activating AMPK to Alleviate Hepatocyte Lipid Accumulation (2023-03-31)
作者:Danqing Wang:1.Savaid Medical School, University of Chinese Academy of Sciences, Beijing 101400, China. ; Miao Zhang:1.Savaid Medical School, University of Chinese Academy of Sciences, Beijing 101400, China. ; Jiaojiao Xu:1.Savaid Medical School, University of Chinese Academy of Sciences, Beijing 101400, China. ; Jianhong Yang:1.Savaid Medical School, University of Chinese Academy of Sciences, Beijing 101400, China. ;
期刊:Uncarboxylated Osteocalcin Decreases SCD1 by Activating AMPK to Alleviate Hepatocyte Lipid Accumulation
影响因子 :4.6
引用产品: NCTC 1469 细胞 , DMEM高糖 培养基 , 马血清
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The Different Mechanisms of Lipid Accumulation in Hepatocytes Induced by Oleic Acid/Palmitic Acid and High-Fat Diet (2023-09-20)
作者:Miao Zhang:1.Savaid Medical School, University of Chinese Academy of Sciences, Beijing 101400, China. ; Xue Bai:1.Savaid Medical School, University of Chinese Academy of Sciences, Beijing 101400, China. ; Qian Du:1.Savaid Medical School, University of Chinese Academy of Sciences, Beijing 101400, China. ; Jiaojiao Xu:1.Savaid Medical School, University of Chinese Academy of Sciences, Beijing 101400, China. ; Danqing Wang:1.Savaid Medical School, University of Chinese Academy of Sciences, Beijing 101400, China. ; Lei Chen:1.Savaid Medical School, University of Chinese Academy of Sciences, Beijing 101400, China. ; Keting Dong:1.Savaid Medical School, University of Chinese Academy of Sciences, Beijing 101400, China. ; Ziyue Chen:1.School of Nursing, Capital Medical University, Beijing 100069, China. ; Jianhong Yang:1.Savaid Medical School, University of Chinese Academy of Sciences, Beijing 101400, China. ;
期刊:The Different Mechanisms of Lipid Accumulation in Hepatocytes Induced by Oleic Acid/Palmitic Acid and High-Fat Diet
影响因子 :4.6
引用产品: NCTC 1469 细胞 , DMEM高糖 培养基 , 马血清
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Effects of protoscoleces excretory-secretory products of Echinococcus granulosus on hepatocyte growth, function, and glucose metabolism (2023-11-08)
作者:Guangyi Luo:1.Section for Hepatopancreatobiliary Surgery, Department of General Surgery, The Third People's Hospital of Chengdu, Affiliated Hospital of Southwest Jiaotong University, Chengdu, 610031, Sichuan, China; Department of Hepatopancreatobiliary Surgery, Anyue County People's Hospital, Ziyang, 642350, Sichuan, China. ; Haiwen Li:1.School of Basic Medicine, Chengdu Medical College, Chengdu, 610500, Sichuan, China. ; Qiong Lu:1.Department of Infectious Diseases, Anyue County People's Hospital, Ziyang, 642350, Sichuan, China. ; Jiangtao Cao:1.School of Basic Medicine, Chengdu Medical College, Chengdu, 610500, Sichuan, China. ; Hailong Lv:1.Section for Hepatopancreatobiliary Surgery, Department of General Surgery, The Third People's Hospital of Chengdu, Affiliated Hospital of Southwest Jiaotong University, Chengdu, 610031, Sichuan, China. Electronic address: lvhl1999@126.com. ; Yufeng Jiang:1.School of Basic Medicine, Chengdu Medical College, Chengdu, 610500, Sichuan, China. El
期刊:Effects of protoscoleces excretory-secretory products of Echinococcus granulosus on hepatocyte growth, function, and glucose metabolism
DOI:10.1016/j.actatropica.2023.107066
影响因子 :2.7
引用产品: NCTC 1469 细胞
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Dietary intake of fructose increases purine de novo synthesis: A crucial mechanism for hyperuricemia (2022-12-19)
作者:Pengfei Zhang:1.Department of Critical Care Medicine, Shenzhen Longhua District Central Hospital, Shenzhen, China. ; 2.Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Shenzhen, China. ; Huimin Sun:1.Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Shenzhen, China. ; Xinyu Cheng:1.Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Shenzhen, China. ; Yajing Li:1.Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Shenzhen, China. ; Yanli Zhao:1.Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Shenzhen, China. ; Wuxuan Mei:1.Clinical Medical College, Hubei University of Science and Technology, Xianning, China. ; Xing Wei:1.Department of Nephrotic Rheumatism, Shenzhen Longhua District Central Hospital, Guangdong Medical University, Shenzhen, China. ; Hairong Zhou:1.Department of General Practice, Shenzhen Longhua District Central Hospital, Guan
期刊:Dietary intake of fructose increases purine de novo synthesis: A crucial mechanism for hyperuricemia
影响因子 :6.6
引用产品: NCTC 1469 细胞
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Periplaneta americana extract alleviates steatohepatitis in a mouse model by modulating HMGB1-mediated inflammatory response (2022-10-03)
作者:Yang Xiao:1.Department of Hepatological Surgery, The First Affiliated Hospital, Jinan University, Guangzhou, China. ; Chongqing Gao:1.Department of Pathophysiology, School of Medicine, Jinan University, Guangzhou, China. ; Junru Wu:1.Department of Pathophysiology, School of Medicine, Jinan University, Guangzhou, China. ; Jing Li:1.Department of Pathophysiology, School of Medicine, Jinan University, Guangzhou, China. ; Lijuan Wang:1.Department of Pathophysiology, School of Medicine, Jinan University, Guangzhou, China. ; Yang You:1.Department of Pathophysiology, School of Medicine, Jinan University, Guangzhou, China. ; Tianqi Peng:1.Department of Hepatological Surgery, The First Affiliated Hospital, Jinan University, Guangzhou, China. ; Keke Zhang:1.Department of Pathophysiology, School of Medicine, Jinan University, Guangzhou, China. ; Mingrong Cao:1.Department of Hepatological Surgery, The First Affiliated Hospital, Jinan University, Guangzhou, China. ; Jian Hong:1.Department
期刊:Periplaneta americana extract alleviates steatohepatitis in a mouse model by modulating HMGB1-mediated inflammatory response
影响因子 :6.0
引用产品: NCTC 1469 细胞 , J774A.1 细胞
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RNA-seq transcriptome profiling of liver regeneration in mice identifies the miR-34b-5p/phosphoinositide-dependent protein kinase 1 axis as a potential target for hepatocyte proliferation (2022-08-21)
作者:Xiaohua Lei:1.The First Affiliated Hospital, Department of Hepatobiliary Surgery, Hengyang Medical School, University of South China, Hengyang, Hunan, China. ; Xiaoming Dai:1.The First Affiliated Hospital, Department of Hepatobiliary Surgery, Hengyang Medical School, University of South China, Hengyang, Hunan, China. ; Qian Wang:1.The First Affiliated Hospital, Department of Reproductive Medicine, Hengyang Medical School, University of South China, Hengyang, Hunan, China. ; Ruchang Long:1.The First Affiliated Hospital, Department of Hepatobiliary Surgery, Hengyang Medical School, University of South China, Hengyang, Hunan, China. ; Zhiqiang Xiang:1.The First Affiliated Hospital, Department of Hepatobiliary Surgery, Hengyang Medical School, University of South China, Hengyang, Hunan, China. ; Huajian Li:1.The First Affiliated Hospital, Department of Hepatobiliary Surgery, Hengyang Medical School, University of South China, Hengyang, Hunan, China. ; Zhangtao Long:1.The First Affili
期刊:RNA-seq transcriptome profiling of liver regeneration in mice identifies the miR-34b-5p/phosphoinositide-dependent protein kinase 1 axis as a potential target for hepatocyte proliferation
DOI:10.1016/j.bbrc.2022.08.049
影响因子 :3.3
引用产品: NCTC 1469 细胞
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Inhibition of GCN2 alleviates hepatic steatosis and oxidative stress in obese mice: Involvement of NRF2 regulation (2012-01-20)
作者:Juntao Yuan:1.College of Life Science, University of Chinese Academy of Sciences, Beijing, 100049, China. ; Zhuoran Yu:1.College of Life Science, University of Chinese Academy of Sciences, Beijing, 100049, China. ; Junling Gao:1.College of Life Science, University of Chinese Academy of Sciences, Beijing, 100049, China. ; Kai Luo:1.College of Life Science, University of Chinese Academy of Sciences, Beijing, 100049, China. ; Xiyue Shen:1.College of Life Science, University of Chinese Academy of Sciences, Beijing, 100049, China. ; Bingqing Cui:1.College of Life Science, University of Chinese Academy of Sciences, Beijing, 100049, China. ; Zhongbing Lu:1.College of Life Science, University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: luzhongbing@ucas.ac.cn. ;
期刊:Inhibition of GCN2 alleviates hepatic steatosis and oxidative stress in obese mice: Involvement of NRF2 regulation
DOI:10.1016/j.redox.2021.102224
影响因子 :11.4
引用产品: NCTC 1469 细胞
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Downregulated long non-coding RNA LINC01093 in liver fibrosis promotes hepatocyte apoptosis via increasing ubiquitination of SIRT1 (2005-00-20)
作者:Yinhe Tang:1.Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Wenzhou Medical University, 205 Wenrui Avenue, Wenzhou 325000, People's Republic of China. ; Naijing Ma:1.Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Wenzhou Medical University, 205 Wenrui Avenue, Wenzhou 325000, People's Republic of China. ; Hao Luo:1.Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Wenzhou Medical University, 205 Wenrui Avenue, Wenzhou 325000, People's Republic of China. ; Shizuan Chen:1.Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Wenzhou Medical University, 205 Wenrui Avenue, Wenzhou 325000, People's Republic of China. ; Fuxiang Yu:1.Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Wenzhou Medical University, 205 Wenrui Avenue, Wenzhou 325000, People's Republic of China. ;
期刊:Downregulated long non-coding RNA LINC01093 in liver fibrosis promotes hepatocyte apoptosis via increasing ubiquitination of SIRT1
影响因子 :2.7
引用产品: NCTC 1469 细胞
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