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产品概述
胰蛋白酶（Trypsin）是一种丝氨酸水解酶，它能把多肽链中赖氨酸和精氨酸残基中的羧基侧切段，水解细胞间的蛋白质，破坏细胞间的连接，从而使组织或贴壁细胞离散成单个细胞。胰酶分散细胞的活性与组织或细胞的特性、胰酶浓度、温度和作用时间有关，在pH 8.0和37℃时，胰酶的作用能力最强，因此使用胰酶时，应把握好浓度、温度和时间，以免消化过度造成细胞损伤。
一般常用胰酶的工作浓度为0.25%，而半贴壁细胞或对胰酶敏感的细胞常采用低浓度（0.05%）的胰酶进行细胞消化。由于EDTA能够螯合Ca2+和Mg2+，从而破坏细胞连接促进细胞的解离，因此在胰酶溶液中常常会加入一定量的EDTA混合使用，以增强解离效果。
产品信息
形态 | 液体 |
规格 | 100mL |
pH | 7.2-7.8 |
溶剂 | D-Hank's |
胰蛋白酶浓度 | 2.5 g/L |
EDTA•2Na | 无 |
酚红指示剂 | 10 mg/L |
储存条件 | -5~-20℃ |
运输条件 | 低温 |
有效期 | 12个月 |
注意事项
1、由于不同的组织或者细胞对胰酶的作用反应不一样，因此操作人员应根据实际情况，确定最佳消化时间；消化细胞时间不宜过长，否则会影响细胞贴壁和生长状况；
2、使用本产品时应注意无菌操作，避免污染；
3、不宜长时间放置于室温环境或2-8℃长期保存；
4、2-8℃中解冻，摇匀后使用，切忌反复冻融，用量较少时建议分装冻存。
5、由于D-Hank's平衡盐溶液NaHCO3含量较低（0.35 g/L），因此该型胰蛋白酶溶液不能用于5% CO2的环境，若放入CO2培养相，溶液将迅速变酸，使用时应注意；
6、本产品中含有酚红且采用干冰运输，因此在运输过程中包装内会有部分二氧化碳累积，这些二氧化碳可能会进入到溶液中导致PH略微下降，从而导致溶液颜色由粉红色变成橙色。该种橙色的溶液依然可以使用或者也可以采用无菌的2 M氢氧化钠溶液对pH进行微调后使用；
7、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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Combined toxicity of oil-based PAH4 mixtures on HL-7702 cells (2023-12-05)
作者：Xiaofang Liu:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; School of Tourism and Cuisine, Yangzhou University, Yangzhou 225127, China. ; Xu Zhang:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. ; Wanli Zhou:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. ; Li Liang:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. ; Jixian Zhang:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. ; Chaoting Wen:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. ; Youdong Li:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. ; Xin Xu:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. ; Guoyan Liu:1.School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China. Electronic address:
期刊：Combined toxicity of oil-based PAH4 mixtures on HL-7702 cells
DOI：10.1016/j.scitotenv.2023.169038
影响因子 ：9.8
引用产品： RPMI-1640 培养基 , 青霉素-链霉素溶液 (双抗)，100× , 特级胎牛血清 , 0.25%胰蛋白酶溶液 (溶于PBS) , 磷酸盐缓冲液 (PBS) 1× , HL-7702 [L-02; LO2] 细胞
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Puerarin improves busulfan-induced disruption of spermatogenesis by inhibiting MAPK pathways (2023-07-27)
作者：Hai-Tao Li:1.Medical School, Institute of Reproductive Medicine, Nantong University, Nantong 226001, Jiangsu, China. ; Kun Zhong:1.Medical School, Institute of Reproductive Medicine, Nantong University, Nantong 226001, Jiangsu, China. ; Yun-Fei Xia:1.Department of Rheumatology, Affiliated Hospital of Nantong University, Nantong University, Nantong 226001, China. ; Jian Song:1.Reproductive Medicine Center, Affiliated Hospital of Nantong University, Nantong University, Nantong 226001, China. ; Xiao-Qing Chen:1.Human Resources Division and Clinical Research Center, Affiliated Hospital of Nantong University, Nantong University, Nantong 226001, China. ; Wei Zhao:1.School of Laboratory Medicine, Chengdu Medical College, Chengdu, China. Electronic address: zw198626520@126.com. ; Xu-Hui Zeng:1.Medical School, Institute of Reproductive Medicine, Nantong University, Nantong 226001, Jiangsu, China. Electronic address: zengxuhui@ntu.edu.cn. ; Tian-Xing Chen:1.Medical School, Institute of Re
期刊：Puerarin improves busulfan-induced disruption of spermatogenesis by inhibiting MAPK pathways
DOI：10.1016/j.biopha.2023.115231
影响因子 ：7.5
引用产品： GC-1 spg 细胞 , TM4 细胞 , DMEM高糖 培养基 , 青霉素-链霉素溶液 (双抗)，100× , 0.25%胰蛋白酶溶液 (含酚红，溶于D-Hank's)
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Near-infrared light-mediated CuS@Rutin nanocomposites for the PTT/PDT synergistic treatment in bacterial infections (2023-12-06)
作者：Jian Liu: 1.Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266003, China;2.School of Stomatology, Qingdao University, Qingdao 266003, China; Baoheng Yin: 1.Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266003, China;2.School of Stomatology, Qingdao University, Qingdao 266003, China; Shuang Li: 1.Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266003, China;2.School of Stomatology, Qingdao University, Qingdao 266003, China; Lian Mi: 1.Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266003, China;2.School of Stomatology, Qingdao University, Qingdao 266003, China; Guanghao Yin: 1.Institute of Stomatology, Binzhou Medical University, Yantai 264003, China; Feng Li: 1.School of Stomatology, Qingdao University, Qingdao 266003, China; Lingmei Zhong: 1.Department of Pulmonary and
期刊：Near-infrared light-mediated CuS@Rutin nanocomposites for the PTT/PDT synergistic treatment in bacterial infections
DOI：10.1016/j.jiec.2023.12.006
影响因子 ：6.1
引用产品： 0.25%胰蛋白酶溶液 (溶于PBS) , DMEM高糖 培养基 , 特级胎牛血清
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Searching for novel MDM2/MDMX dual inhibitors through a drug repurposing approach (2023-12-07)
作者：Keting Li:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ; Wenshu Hu:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ; Yingjie Wang:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ; Wenxing Chen:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ; Hongmei Wen:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ; Jian Liu:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ; Wei Li:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ; Bo Wang:1.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China. ;
期刊：Searching for novel MDM2/MDMX dual inhibitors through a drug repurposing approach
DOI：10.1080/14756366.2023.2288810
影响因子 ：5.6
引用产品： DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100× , 0.25%胰蛋白酶溶液 (溶于PBS)
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Analysis of the mechanism of curcumin against osteoarthritis using metabolomics and transcriptomics (2023-11-08)
作者：Wenxiang Deng:1.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, Hunan, China. ; Qinghu He:1.Department of Rehabilitation and Healthcare, Hunan University of Medicine, Huaihua, 418000, Hunan, China. hqh19651111@dingtalk.com. ; 2.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, Hunan, China. hqh19651111@dingtalk.com. ; Wenan Zhang:1.College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, Hunan, China. ;
期刊：Analysis of the mechanism of curcumin against osteoarthritis using metabolomics and transcriptomics
DOI：10.1007/s00210-023-02785-y
影响因子 ：3.6
引用产品： 人关节软骨细胞 , 人关节软骨细胞完全培养基 , 0.25%胰蛋白酶溶液 (溶于PBS)
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Ubenimex suppresses glycolysis mediated by CD13/Hedgehog signaling to enhance the effect of cisplatin in liver cancer (2023-09-28)
作者：Yunyan Shi:1.Department of Pharmacology, Qingdao University, Qingdao, China. ; Qie Guo:1.Department of Clinical Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Fanjing Jing:1.Department of Lymphoma, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Xiuling Shang:1.Department of Clinical Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Changkai Zhou:1.Department of Clinical Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, China. ; Fanbo Jing:1.Department of Clinical Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, China. ;
期刊：Ubenimex suppresses glycolysis mediated by CD13/Hedgehog signaling to enhance the effect of cisplatin in liver cancer
影响因子 ：0.9
引用产品： DMEM高糖 培养基 , MEM 培养基 , 特级胎牛血清 , 0.25%胰蛋白酶溶液 (溶于PBS)
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