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产品概述
名称 | MNNG/HOS Cl #5 [R-1059-D] (人骨肉瘤细胞) (STR鉴定正确) |
别称 | MNNG/HOS; MNNG-HOS; HOS-MNNG; MNNGHOS; MNNG-HOS (Cl#5) ; MNNG/HOS Clone F-5; MNNG; R-1059-D; TE85; TE-85; HOS-TE85; Hos TE-85; HOS TE 85; HOS TE85; HOS (TE85) ; HOS (TE85) ; HOS (TE85, Clone F5) ; MNNG-HOS (TE 85, clone F-5) ; HOS-Te85; TE 85.T; TE 85 ClF-5; TE-85 clone 5 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | MNNG/HOS Cl #5 [R-1059-D]细胞是HOS经0.01mcg/ml MNNG(一种致癌的亚硝胺)转化而来。MNNG/HOS Cl #5 [R-1059-D]细胞饱和浓度高,在软琼脂中的克隆形成率高,并能在裸鼠中成瘤。 |
年龄(性别) | 女性;13岁 |
组织来源 | 骨;骨肉瘤 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肉瘤细胞 |
生物安全等级 | BSL-1 |
保藏机构 | ATCC; CRL-1547 ECACC; 87070201 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 24,25 D3S1358 15 D5S818 13 D7S820 11,12 D8S1179 11,14 D13S317 12 D16S539 10,13 D18S51 14 D19S433 13 D21S11 31.2 FGA 24 PentaD 9,10 PentaE 7,12 TH01 6 TPOX 8,11 vWA 18 D6S1043 18 D12S391 20 D2S441 10 -
STR鉴定图
参考文献
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Circular RNA circ_0096041 promotes osteosarcoma cell proliferation and migration via sponging miR-556-5p and regulating LIN28A expression (2024-02-29)
作者:Fulin Zhou:1.Department of Orthopedics, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu 213003, China. zfulin@gmail.com. ; Hao Wu:1.Department of Orthopedics, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu 213003, China. wuyu9967@163.com. ; Zhen Yin:1.Department of Orthopedics, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu 213003, China. 18852861751@163.com. ; Weibo Zhou:1.Department of Orthopedics, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu 213003, China. zwb-2008@163.com. ; Wei Chen:1.Department of Orthopedics, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu 213003, China. 13641502368@163.com. ;
期刊:Circular RNA circ_0096041 promotes osteosarcoma cell proliferation and migration via sponging miR-556-5p and regulating LIN28A expression
影响因子 :1.6
引用产品: MNNG/HOS Cl #5 [R-1059-D] 细胞 , U-2 OS [U2OS] 细胞 , MG-63 细胞 , hFOB 1.19 细胞
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2-Hydroxy-3-methylanthraquinone inhibits homologous recombination repair in osteosarcoma through the MYC-CHK1-RAD51 axis (2023-01-30)
作者:Doudou Jing:1.Department of Orthopaedics, The Second Hospital of Shanxi Medical University, Taiyuan, 030001, China. ; 2.Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; Xuanzuo Chen:1.Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; Zhenhao Zhang:1.Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; Fengxia Chen:1.Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China. ; Fuhua Huang:1.Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; Zhicai Zhang:1.Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. ; We
期刊:2-Hydroxy-3-methylanthraquinone inhibits homologous recombination repair in osteosarcoma through the MYC-CHK1-RAD51 axis
DOI:10.1186/s10020-023-00611-y
影响因子 :5.7
引用产品: MNNG/HOS Cl #5 [R-1059-D] 细胞 , U-2 OS [U2OS] 细胞 , MNNG/HOS Cl #5 [R-1059-D]细胞专用培养基 , U-2 OS [U2OS]细胞专用培养基
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PNO1 promotes the progression of osteosarcoma via TGF-β and YAP/TAZ pathway (2023-12-09)
作者:Long Fang:1.Department of Bone and Soft Tissue Tumors, Shandong Provincial Third Hospital, Shandong University, Jinan, 250000, China. ; Baolong Wang:1.Department of Bone and Soft Tissue Tumors, Shandong Provincial Third Hospital, Shandong University, Jinan, 250000, China. ; Zengkun Yang:1.Department of Bone and Soft Tissue Tumors, Shandong Provincial Third Hospital, Shandong University, Jinan, 250000, China. ; Tingbao Zhao:1.Department of Bone and Soft Tissue Tumors, Shandong Provincial Third Hospital, Shandong University, Jinan, 250000, China. ; Wei Hao:1.Department of Orthopedics and Traumatology, Shandong Provincial Third Hospital, Shandong University, Jinan, 250000, China. ehaw@163.com. ;
期刊:PNO1 promotes the progression of osteosarcoma via TGF-β and YAP/TAZ pathway
DOI:10.1038/s41598-023-49295-8
影响因子 :4.6
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Prognosis-related VDAC1 regulates the proliferation and apoptosis of osteosarcoma cells via the MAPK signaling pathway (2023-03-05)
作者:Xu Cai:1.Department of Orthopedics, The Third Xiangya Hospital of Central South University, Changsha 410000, China. ; Shaihong Zhu:1.Department of General Surgery, the Third Xiangya Hospital of Central South University, Changsha 410000, China. Electronic address: shongzhu@163.com. ;
期刊:Prognosis-related VDAC1 regulates the proliferation and apoptosis of osteosarcoma cells via the MAPK signaling pathway
DOI:10.1016/j.ygeno.2023.110595
影响因子 :4.4
引用产品: hFOB 1.19 细胞 , MNNG/HOS Cl #5 [R-1059-D] 细胞 , MG-63 细胞 , 143B 细胞 , U-2 OS [U2OS] 细胞
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RING finger gene 180 inhibits osteosarcoma progression through regulating chromobox homolog 4 ubiquitination (2023-04-24)
作者:Qirui Zhao:1.Department of Orthopedic Joint and Sports Medicine Ward, The First Clinical Hospital affiliated to Harbin Medical University, Harbin, Heilongjiang, China. ; Ning Liu:1.Department of Orthopedic Joint and Sports Medicine Ward, The First Clinical Hospital affiliated to Harbin Medical University, Harbin, Heilongjiang, China. ; Tongtong Xu:1.Major of Nursing, China Medical University, Shenyang, Liaoning, China. ; Keguan Song:1.Department of Orthopedic Joint and Sports Medicine Ward, The First Clinical Hospital affiliated to Harbin Medical University, Harbin, Heilongjiang, China. ;
期刊:RING finger gene 180 inhibits osteosarcoma progression through regulating chromobox homolog 4 ubiquitination
DOI:10.1080/15384101.2023.2205201
影响因子 :4.3
引用产品: 293T [HEK-293T] 细胞 , hFOB 1.19 细胞 , MNNG/HOS Cl #5 [R-1059-D] 细胞 , Saos-2 细胞 , MG-63 细胞 , U-2 OS [U2OS] 细胞
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Identification of an EMT-related gene-based prognostic signature in osteosarcoma (2023-04-27)
作者:Haoli Gong:1.Department of Orthopedics, Hunan Provincial People's Hospital (The First-Affiliated Hospital of Hunan Normal University), Changsha, China. ; Ye Tao:1.Department of Radiology, The Third Xiangya Hospital, Central South University, Hunan, Changsha, China. ; Sheng Xiao:1.Department of Orthopedics, Hunan Provincial People's Hospital (The First-Affiliated Hospital of Hunan Normal University), Changsha, China. ; Xin Li:1.Department of Orthopedics, Hunan Provincial People's Hospital (The First-Affiliated Hospital of Hunan Normal University), Changsha, China. ; Ke Fang:1.Department of Orthopedics, Hunan Provincial People's Hospital (The First-Affiliated Hospital of Hunan Normal University), Changsha, China. ; Jie Wen:1.Department of Orthopedics, Hunan Provincial People's Hospital (The First-Affiliated Hospital of Hunan Normal University), Changsha, China. ; Ming Zeng:1.Department of Orthopedics, Hunan Provincial People's Hospital (The First-Affiliated Hospital of Hunan Normal
期刊:Identification of an EMT-related gene-based prognostic signature in osteosarcoma
影响因子 :4.0
引用产品: U-2 OS [U2OS] 细胞 , MNNG/HOS Cl #5 [R-1059-D] 细胞 , McCoy's 5A 培养基 , MEM 培养基
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A short peptide LINC00665_18aa encoded by lncRNA LINC00665 suppresses the proliferation and migration of osteosarcoma cells through the regulation of the CREB1/RPS6KA3 interaction (2023-06-07)
作者:Junwei Pan:1.Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ; Ming Liu:1.Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ; Xiaozong Duan:1.Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ; Dan Wang:1.Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. ;
期刊:A short peptide LINC00665_18aa encoded by lncRNA LINC00665 suppresses the proliferation and migration of osteosarcoma cells through the regulation of the CREB1/RPS6KA3 interaction
DOI:10.1371/journal.pone.0286422
影响因子 :3.7
引用产品: MNNG/HOS Cl #5 [R-1059-D] 细胞 , U-2 OS [U2OS] 细胞 , DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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Disulfidptosis-related PABPC3 promotes tumor progression and inhibits immune activity in osteosarcoma (2023-12-07)
作者:Yangbo Cao:1.Department of Orthopaedics, The Third Xiangya Hospital, Central South University, Changsha, China. ; Song Wu:1.Department of Orthopaedics, The Third Xiangya Hospital, Central South University, Changsha, China. ; Yishan Gu:1.Division of Life Science and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Hong Kong, China. ; Yung Hou Wong:1.Division of Life Science and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Hong Kong, China. ; Yanbin Shi:1.Department of Orthopaedics, The Third Xiangya Hospital, Central South University, Changsha, China. ; Lina Zhang:1.Department of Orthopaedics, Hunan Provincial People's Hospital, Hunan Normal University, Changsha, China. ;
期刊:Disulfidptosis-related PABPC3 promotes tumor progression and inhibits immune activity in osteosarcoma
DOI:10.1002/jgm.3641
影响因子 :3.5
引用产品: U-2 OS [U2OS] 细胞 , MNNG/HOS Cl #5 [R-1059-D] 细胞 , McCoy's 5A 培养基 , MEM 培养基
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Diverse cell death patterns-related signature for predicting prognosis and drug sensitivity of osteosarcoma patients (2023-10-20)
作者:Yanbin Shi:1.Department of Orthopaedics, The Third Xiangya Hospital, Central South University, Changsha, China. ; Song Wu:1.Department of Orthopaedics, The Third Xiangya Hospital, Central South University, Changsha, China. ; Xiaolin Zhang:1.The Third Xiangya Hospital, Central South University, Changsha, China. ; Yangbo Cao:1.Department of Orthopaedics, The Third Xiangya Hospital, Central South University, Changsha, China. ; Lina Zhang:1.Hunan Provincial People's Hospital, Changsha, China. ;
期刊:Diverse cell death patterns-related signature for predicting prognosis and drug sensitivity of osteosarcoma patients
DOI:10.1002/jgm.3613
影响因子 :3.5
引用产品: U-2 OS [U2OS] 细胞 , MNNG/HOS Cl #5 [R-1059-D] 细胞 , McCoy's 5A 培养基 , MEM 培养基
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Engineered biomimetic nanoreactor for synergistic photodynamic-chemotherapy against hypoxic tumor (2022-09-22)
作者:Haoyu Guo:1.Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. ; Lutong Wang:1.Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. ; Wei Wu:1.Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. ; Mingke Guo:1.Department of Orthopaedics, Affiliated Hospital of NCO school of Army Medical University, Shijiazhuang 050041, China. ; Lingkai Yang:1.Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. ; Zhenhao Zhang:1.Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. ; Li Cao:1.Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Scien
期刊:Engineered biomimetic nanoreactor for synergistic photodynamic-chemotherapy against hypoxic tumor
DOI:10.1016/j.jconrel.2022.09.020
影响因子 :11.5
引用产品: MNNG/HOS Cl #5 [R-1059-D] 细胞 , U-2 OS [U2OS] 细胞 , 人脐静脉内皮细胞 , RAW 264.7 细胞
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Chitosan based photothermal scaffold fighting against bone tumor-related complications: Recurrence, infection, and defects (2022-10-26)
作者:Yao Zhao:1.College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. ; Xu Peng:1.College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China; Experimental and Research Animal Institute, Sichuan University, Chengdu 610065, China. ; Xiaoyang Xu:1.College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. ; Mingzhen Wu:1.College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. ; Fan Sun:1.College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China. ; Qiangwei Xin:1.College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan Univers
期刊:Chitosan based photothermal scaffold fighting against bone tumor-related complications: Recurrence, infection, and defects
DOI:10.1016/j.carbpol.2022.120264
影响因子 :10.7
引用产品: 小鼠骨髓间充质干细胞 , MG-63 细胞 , MNNG/HOS Cl #5 [R-1059-D] 细胞
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A ferroptosis-related gene signature associated with immune landscape and therapeutic response in osteosarcoma. (2022-11-11)
作者:Xinxing Wang:1.Department of Orthopaedics, The Third Xiangya Hospital, Central South University, Changsha, China. ; Guang Xia:1.Department of Orthopaedics, The Third Xiangya Hospital, Central South University, Changsha, China. ; Shilang Xiao:1.Department of Gastroenterology, The Third Xiangya Hospital, Central South University, Changsha, China. ; Song Wu:1.Department of Orthopaedics, The Third Xiangya Hospital, Central South University, Changsha, China. ; Lina Zhang:1.Department of Orthopaedics, The Third Xiangya Hospital, Central South University, Changsha, China. ; Junjie Huang:1.Department of Orthopaedics, The Third Xiangya Hospital, Central South University, Changsha, China. ; Wenxiu Zhang:1.Department of Orthopaedics, The Third Xiangya Hospital, Central South University, Changsha, China. ; Xu Cao:1.Department of Orthopaedics, The Third Xiangya Hospital, Central South University, Changsha, China. ;
期刊:A ferroptosis-related gene signature associated with immune landscape and therapeutic response in osteosarcoma.
影响因子 :5.7
引用产品: U-2 OS [U2OS] 细胞 , MNNG/HOS Cl #5 [R-1059-D] 细胞 , McCoy's 5A 培养基 , MEM 培养基
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