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产品概述
名称 | Saos-2 (人成骨肉瘤细胞) (STR鉴定正确) |
别称 | SAOS-2; Saos-2; SAOS 2; Saos 2; Saos2; SaOs2; SAOS2; Sarcoma OSteogenic-2; SaOS; SAOS |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:3 |
推荐换液频率 | 2-3次/周 |
背景描述 | Saos-2细胞是由J·Fogh和G·Trempe分离和鉴定的众多人肿瘤细胞系中的一种。该病人曾经多种药物治疗,包括RTG、methotrexate、adriamycin vincristine、cytoxan和aramycin-C。Saos-2细胞在免疫抑制小鼠中不致瘤,但在半固体培养基中形成集落。 |
年龄(性别) | 女性;11岁 |
组织来源 | 骨;骨肉瘤 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肉瘤细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~48 hours |
致瘤性 | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
受体表达情况 | epidermal growth factor (EGF); transforming growth factor beta (Type 1 and Type 2) |
抗原表达情况 | Blood Type B, Rh+; HLA A2, A3, Bw16, Bw47 |
保藏机构 | ATCC; CRL-7939ATCC; HTB-85 DSMZ; ACC-243 ECACC; 89050205 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10 D2S1338 18 D3S1358 18 D5S818 12 D7S820 8,10 D8S1179 10,12 D13S317 12,13 D16S539 12,13 D18S51 15 D19S433 13 D21S11 28,30 FGA 22,25 PentaD 11,12 PentaE 14,19 TH01 6,9 TPOX 8 vWA 18 D6S1043 11,14 D12S391 20 D2S441 10,14 -
STR鉴定图
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参考文献
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Single-Atom Cu Nanozyme-Loaded Bone Scaffolds for Ferroptosis-Synergized Mild Photothermal Therapy in Osteosarcoma Treatment (2024-02-29)
期刊:Advanced Healthcare Materials
影响因子 :10.0
引用产品: Saos-2 细胞
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Single-cell and spatial transcriptomics reveal metastasis mechanism and microenvironment remodeling of lymph node in osteosarcoma (2024-05-17)
期刊:BMC Medicine
DOI:10.1186/s12916-024-03319-w
影响因子 :9.3
引用产品: hFOB 1.19 细胞 , 143B 细胞 , Saos-2 细胞
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Multi-omics analysis reveals cuproptosis and mitochondria-based signature for assessing prognosis and immune landscape in osteosarcoma (2024-01-05)
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2023.1280945
影响因子 :7.3
引用产品: hFOB 1.19 细胞 , HOS 细胞 , Saos-2 细胞 , 143B 细胞
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Soy isoflavones induces mitophagy to inhibit the progression of osteosarcoma by blocking the AKT/mTOR signaling pathway (2024-01-08)
期刊:MOLECULAR MEDICINE
DOI:10.1186/s10020-024-00778-y
影响因子 :5.7
引用产品: U-2 OS [U2OS] 细胞 , Saos-2 细胞 , MG-63 细胞
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Comprehensive investigation of tumor immune microenvironment and prognostic biomarkers in osteosarcoma through integrated bulk and single-cell transcriptomic analysis (2024-07-23)
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2024.1424950
影响因子 :5.7
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Sirtuin 3-activated superoxide dismutase 2 mediates fluoride-induced osteoblastic differentiation in vitro and in vivo by down-regulating reactive oxygen species (2024-07-16)
期刊:ARCHIVES OF TOXICOLOGY
DOI:10.1007/s00204-024-03819-x
影响因子 :4.8
引用产品: McCoy's 5A 培养基 , MG-63 细胞 , Saos-2 细胞 , MG-63细胞专用培养基
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IMPC-based screening revealed that ROBO1 can regulate osteoporosis by inhibiting osteogenic differentiation (2024-10-08)
期刊:Frontiers in Cell and Developmental Biology
DOI:10.3389/fcell.2024.1450215
影响因子 :4.6
引用产品: Saos-2 细胞 , 小鼠骨髓间充质干细胞 , MC3T3-E1 Subclone 14 细胞
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CircPRMT5 promotes progression of osteosarcoma by recruiting CNBP to regulate the translation and stability of CDK6 mRNA (2024-04-16)
期刊:PLoS One
DOI:10.1371/journal.pone.0298947
影响因子 :3.7
引用产品: hFOB 1.19 细胞 , Saos-2 细胞
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MiR-150-5p inhibits cell proliferation and metastasis by targeting FTO in osteosarcoma (2024-10-16)
期刊:ONCOLOGY RESEARCH
影响因子 :2.0
引用产品: MG-63 细胞 , HOS 细胞 , Saos-2 细胞 , U-2 OS [U2OS] 细胞 , hFOB 1.19 细胞
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Bone-targeting exosome nanoparticles activate Keap1 / Nrf2 / GPX4 signaling pathway to induce ferroptosis in osteosarcoma cells (2023-09-30)
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-023-02129-1
影响因子 :10.2
引用产品: 143B 细胞 , MG-63 细胞 , Saos-2 细胞 , U-2 OS [U2OS] 细胞 , 人骨髓间充质干细胞
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Natural Chlorogenic Acid Planted Nanohybrids with Steerable Hyperthermia for Osteosarcoma Suppression and Bone Regeneration (2023-05-11)
期刊:Advanced Healthcare Materials
影响因子 :10.0
引用产品: Saos-2 细胞 , MC3T3-E1 Subclone 24 细胞
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Osteoporotic bone loss from excess iron accumulation is driven by NOX4-triggered ferroptosis in osteoblasts (2023-02-03)
期刊:FREE RADICAL BIOLOGY AND MEDICINE
DOI:10.1016/j.freeradbiomed.2023.01.026
影响因子 :7.4
引用产品: hFOB 1.19 细胞 , Saos-2 细胞
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Exploring the immune microenvironment of osteosarcoma through T cell exhaustion-associated gene expression: a study on prognosis prediction (2023-12-15)
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2023.1265098
影响因子 :7.3
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A1BG-AS1 promotes the biological functions of osteosarcoma cells via regulating the microRNA-148a-3p/USP22 axis and stabilizing the expression of SIRT1 through deubiquitinase function (2023-10-03)
期刊:EXPERT OPINION ON THERAPEUTIC TARGETS
DOI:10.1080/14728222.2023.2263908
影响因子 :5.8
引用产品: U-2 OS [U2OS] 细胞 , Saos-2 细胞 , MG-63 细胞 , HOS 细胞 , SJSA-1 细胞 , hFOB 1.19 细胞 , McCoy's 5A 培养基 , MEM 培养基 , RPMI-1640 培养基 , DMEM/F12 培养基
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ATRX loss suppresses the type I interferon response in sarcoma cells through chromatin remodeling (2023-08-15)
期刊:American Journal of Cancer Research
DOI:PMID:37693131
影响因子 :5.3
引用产品: HOS 细胞 , U-2 OS [U2OS] 细胞 , Saos-2 细胞 , 293T [HEK-293T] 细胞 , MG-63 细胞
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Circular RNA hsa_circ_001350 contributes to osteosarcoma progression by regulating microRNA-578/CCR4-NOT transcription complex and subunit 7/Wnt signaling (2023-06-15)
期刊:American Journal of Cancer Research
DOI:PMID:37424831
影响因子 :5.3
引用产品: hFOB 1.19 细胞 , U-2 OS [U2OS] 细胞 , HOS 细胞 , Saos-2 细胞 , MG-63 细胞
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HTRA3 transcriptionally inhibited by FOXP1 suppresses tumorigenesis of osteosarcoma via the PTEN/PI3K/AKT pathway (2023-07-30)
期刊:BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
DOI:10.1016/j.bbamcr.2023.119553
影响因子 :5.1
引用产品: HOS 细胞 , Saos-2 细胞 , SW 1353 细胞 , McCoy's 5A 培养基
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RING finger gene 180 inhibits osteosarcoma progression through regulating chromobox homolog 4 ubiquitination (2023-04-24)
期刊:CELL CYCLE
DOI:10.1080/15384101.2023.2205201
影响因子 :4.3
引用产品: 293T [HEK-293T] 细胞 , hFOB 1.19 细胞 , MNNG/HOS Cl #5 [R-1059-D] 细胞 , Saos-2 细胞 , MG-63 细胞 , U-2 OS [U2OS] 细胞
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hsa_circ_0020378 regulating miR-339-3p/COL1A1 promotes osteosarcoma progression (2023-11-18)
期刊:CANCER BIOLOGY & THERAPY
DOI:10.1080/15384047.2023.2274120
影响因子 :3.6
引用产品: hFOB 1.19 细胞 , HOS 细胞 , Saos-2 细胞 , SW 1353 细胞 , Leibovitz's L-15 培养基 , McCoy's 5A完全培养基 (含10%FBS) , MEM 培养基 , DMEM/F12 培养基 , 青霉素-链霉素溶液 (双抗),100× , 特级胎牛血清
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Exosomal miR-331-3p derived from chemoresistant osteosarcoma cells induces chemoresistance through autophagy (2023-11-22)
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-023-04338-8
影响因子 :2.6
引用产品: MG-63 细胞 , HOS 细胞 , U-2 OS [U2OS] 细胞 , Saos-2 细胞
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DEPDC1 and KIF4A synergistically inhibit the malignant biological behavior of osteosarcoma cells through Hippo signaling pathway (2023-02-27)
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-023-03572-4
影响因子 :2.6
引用产品: hFOB 1.19 细胞 , U-2 OS [U2OS] 细胞 , Saos-2 细胞 , MG-63 细胞
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Developing a Versatile Multiscale Therapeutic Platform for Osteosarcoma Synergistic Photothermo-Chemotherapy with Effective Osteogenicity and Antibacterial Capability (2022-09-20)
期刊:ACS Applied Materials & Interfaces
影响因子 :10.4
引用产品: Saos-2 细胞 , MDA-MB-231 细胞
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Comprehensive analysis of a glycolysis and cholesterol synthesis-related genes signature for predicting prognosis and immune landscape in osteosarcoma (2022-12-23)
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2022.1096009
影响因子 :8.8
引用产品: MG-63 细胞 , U-2 OS [U2OS] 细胞 , Saos-2 细胞 , HOS 细胞 , hFOB 1.19 细胞
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CKB affects human osteosarcoma progression by regulating the p53 pathway (2022-10-15)
期刊:American Journal of Cancer Research
DOI:PMID:36381321
影响因子 :5.9
引用产品: U-2 OS [U2OS] 细胞 , hFOB 1.19 细胞 , Saos-2 细胞 , HOS 细胞 , MG-63 细胞
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MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm (2021-03-04)
期刊:cell death and differentiation
DOI:10.1038/s41418-021-00744-9
影响因子 :12.4
引用产品: Saos-2 细胞 , McCoy's 5A 培养基
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lncRNA MELTF-AS1 facilitates osteosarcoma metastasis by modulating MMP14 expression (2021-08-26)
期刊:Molecular Therapy-Nucleic Acids
DOI:10.1016/j.omtn.2021.08.022
影响因子 :8.8
引用产品: Saos-2 细胞 , MG-63 细胞 , U-2 OS [U2OS] 细胞 , HOS 细胞
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Quantitative phosphoproteomic analysis reveals chemoresistance-related proteins and signaling pathways induced by rhIL-6 in human osteosarcoma cells (2021-10-30)
期刊:Cancer Cell International
DOI:10.1186/s12935-021-02286-z
影响因子 :5.8
引用产品: Saos-2 细胞
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Down-regulation of PRR11 affects the proliferation, migration and invasion of osteosarcoma by inhibiting the Wnt/β-catenin pathway (2021-09-13)
期刊:Journal of Cancer
影响因子 :3.9
引用产品: Saos-2 细胞 , MG-63 细胞 , U-2 OS [U2OS] 细胞 , hFOB 1.19 细胞
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Schisandrin B Inhibits Cell Viability and Migration, and Induces Cell Apoptosis by circ_0009112/miR-708-5p Axis Through PI3K/AKT Pathway in Osteosarcoma (2021-12-22)
期刊:Frontiers in Genetics
影响因子 :3.7
引用产品: U-2 OS [U2OS] 细胞 , Saos-2 细胞 , hFOB 1.19 细胞
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Ginsenoside Rg3 inhibits osteosarcoma progression by reducing circ_0003074 expression in a miR-516b-5p/KPNA4-dependent manner (2021-12-20)
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-021-02868-7
影响因子 :2.6
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circATP2A2 promotes osteosarcoma progression by upregulating MYH9 (2021-11-22)
期刊:Open Medicine
影响因子 :2.1
引用产品: U-2 OS [U2OS] 细胞 , HOS 细胞 , Saos-2 细胞 , 特级胎牛血清 , DMEM/F12 培养基 , hFOB 1.19 细胞
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ADAM10 promotes cell growth, migration, and invasion in osteosarcoma via regulating E-cadherin/β-catenin signaling pathway and is regulated by miR-122-5p (2020-03-30)
期刊:Cancer Cell International
DOI:10.1186/s12935-020-01174-2
影响因子 :5.8
引用产品: Saos-2 细胞 , SW 1353 细胞 , HOS 细胞 , McCoy's 5A 培养基
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Antitumor Effect of miR-1294/Pyruvate Kinase M2 Signaling Cascade in Osteosarcoma Cells (2020-02-21)
期刊:OncoTargets and Therapy
影响因子 :4.0
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Long Non-Coding RNA OIP5-AS1 Knockdown Enhances CDDP Sensitivity in Osteosarcoma via miR-377-3p/FOSL2 Axis (2020-05-07)
期刊:OncoTargets and Therapy
影响因子 :4.0
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Sirt1 modulates H3 phosphorylation and facilitates osteosarcoma cell autophagy (2019-08-07)
期刊:Artificial Cells Nanomedicine and Biotechnology
DOI:10.1080/21691401.2019.1648280
影响因子 :5.8
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