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产品概述
名称 | NCI-H522[H522] (人非小细胞肺癌细胞) (STR鉴定正确) |
别称 | NCI.H522; H522; H-522; NCI-522; NCI522; NCIH522 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:6 |
推荐换液频率 | 2-3次/周 |
背景描述 | NCI-H522 [H522] is a cell line exhibiting epithelial morphology that were isolated from the lungs of a 58-year-old White male with adenocarcinoma. |
年龄(性别) | 男性;58岁 |
组织来源 | 肺 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | 38.2 hours (NCI-DTP=NCI-H522) |
保藏机构 | ATCC; CRL-5810 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,12 D2S1338 17,20 D3S1358 16 D5S818 11 D7S820 9,10 D8S1179 12,13 D13S317 10 D16S539 12 D18S51 14,18 D19S433 16 D21S11 28,31.2 FGA 24 PentaD 11 PentaE 11 TH01 7,9.3 TPOX 9,10 vWA 17 D6S1043 12 D12S391 17 D2S441 10,14 -
STR鉴定图
参考文献
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Circ_0006324 regulates cell proliferation, cell-cycle progression, apoptosis, and glycolysis of non-small cell lung cancer cells through miR-496/TRIM59 axis (2023-08-07)
作者:Tao Wang:1.Department of Thoracic surgery, Affiliated hospital of Guizhou medical university, Guiyang, Guizhou, China. ; Xu Zhu:1.Department of Thoracic surgery, Affiliated hospital of Guizhou medical university, Guiyang, Guizhou, China. ; Kai Wang:1.Department of Thoracic surgery, Affiliated hospital of Guizhou medical university, Guiyang, Guizhou, China. ; Ronghai Ding:1.Department of Basic Medicine, Guizhou Medical university, Guiyang, Guizhou, China. ;
期刊:Circ_0006324 regulates cell proliferation, cell-cycle progression, apoptosis, and glycolysis of non-small cell lung cancer cells through miR-496/TRIM59 axis
影响因子 :3.6
引用产品: A549 [A-549] 细胞 , NCI-H1975 细胞 , NCI-H522[H522] 细胞 , HCC827 细胞 , BEAS-2B 细胞
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circ_0000376 knockdown suppresses non-small cell lung cancer cell tumor properties by the miR-545-3p/PDPK1 pathway (2023-02-16)
作者:Changpeng Sun:1.Department of Cardiothoracic Surgery, Jianhu Clinical Medical College of Yangzhou University, No. 666, Nanhuan Road, Jinhu Town, Jianhu, Yancheng City, Jiangsu Province, 224700, PR China. ; Hongjun Guan:1.Department of Cardiothoracic Surgery, Jianhu Clinical Medical College of Yangzhou University, Yancheng City, Jiangsu Province, 224700, PR China. ; Jinjin Li:1.Department of Cardiothoracic Surgery, Jianhu Clinical Medical College of Yangzhou University, Yancheng City, Jiangsu Province, 224700, PR China. ; Yinfeng Gu:1.Department of Cardiothoracic Surgery, Jianhu Clinical Medical College of Yangzhou University, Yancheng City, Jiangsu Province, 224700, PR China. ;
期刊:circ_0000376 knockdown suppresses non-small cell lung cancer cell tumor properties by the miR-545-3p/PDPK1 pathway
影响因子 :2.1
引用产品: 16HBE 细胞 , NCI-H522[H522] 细胞 , A549 [A-549] 细胞 , RPMI-1640 培养基 , Ham's F-12K 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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