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产品概述
名称 | HCC827 (人非小细胞肺癌细胞) (STR鉴定正确) |
别称 | HCC-827; HCC0827 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | HCC827细胞是于1994年3月建系,组织提供者在25岁到26岁时每个月抽1包烟,在诊断前12年不再抽烟。HCC827细胞在EGFR酪氨酸激酶区域有一个获得性突变(E746-A750缺失)。 |
年龄(性别) | 女性;39岁 |
组织来源 | 肺腺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~28-60 hours |
保藏机构 | ATCC; CRL-2868 BCRJ; 0351 DSMZ; ACC-566 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11 D2S1338 17,24 D3S1358 17 D5S818 12 D7S820 11,12 D8S1179 12 D13S317 9 D16S539 12 D18S51 13 D19S433 14 D21S11 31 FGA 22,24 PentaD 14 PentaE 20 TH01 6 TPOX 8 vWA 18 D6S1043 11,12 D12S391 17 D2S441 11,11.3 -
STR鉴定图
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参考文献
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UPP1 promotes lung adenocarcinoma progression through the induction of an immunosuppressive microenvironment (2024-02-08)
期刊:Nature Communications
DOI:10.1038/s41467-024-45340-w
影响因子 :16.6
引用产品: HCC827 细胞 , LLC [LL/2; LLC1] 细胞 , HFL1 细胞 , THP-1 细胞 , Ham's F-12K 培养基 , HFL1细胞完全培养基
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Targeting TYK2 alleviates Rab27A-induced malignant progression of non-small cell lung cancer via disrupting IFNα-TYK2-STAT-HSPA5 axis (2024-03-23)
期刊:npj Precision Oncology
DOI:10.1038/s41698-024-00574-1
影响因子 :7.9
引用产品: 293T [HEK-293T] 细胞 , BEAS-2B 细胞 , NCI-H1299 细胞 , A549 [A-549] 细胞 , LTEP-α-2 细胞 , NCI-H226 [H226] 细胞 , Calu-3 细胞 , HCC827 细胞 , NCI-H1975 细胞
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NEAT1_1 confers gefitinib resistance in lung adenocarcinoma through promoting AKR1C1-mediated ferroptosis defence (2024-03-12)
期刊:Cell Death Discovery
DOI:10.1038/s41420-024-01892-w
影响因子 :7.0
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KIAA1429-mediated RXFP1 attenuates non-small cell lung cancer tumorigenesis via N6-methyladenosine modification (2024-02-07)
期刊:Cancer Biomarkers
影响因子 :3.1
引用产品: BEAS-2B 细胞 , HCC827 细胞 , PC-9 细胞 , RPMI-1640 培养基 , DMEM高糖 培养基 , 特级胎牛血清
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Antioncogenic roles of USP9Y and DDX3Y in lung cancer: USP9Y stabilizes DDX3Y by preventing its degradation through deubiquitination (2024-01-12)
期刊:ACTA HISTOCHEMICA
DOI:10.1016/j.acthis.2023.152132
影响因子 :2.5
引用产品: BEAS-2B 细胞 , HCC827 细胞 , A549 [A-549] 细胞 , SPC-A-1 细胞 , NCI-H460 [H460] 细胞
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Anticancer effect of covalent purine-containing EGFR TKI, ZZC4 and its mechanism of action through network pharmacology (2023-11-27)
期刊:LIFE SCIENCES
影响因子 :6.1
引用产品: HCC827 细胞 , NCI-H1975 细胞
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Celastrus orbiculatus Thunb. extract targeting DJ-1 inhibits non-small cell lung cancer invasion and metastasis through mitochondrial-induced ROS accumulation (2023-07-20)
期刊:JOURNAL OF ETHNOPHARMACOLOGY
影响因子 :5.4
引用产品: NCI-H226 [H226] 细胞 , NCI-H520 细胞 , HCC827 细胞
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Rapid detection of EGFR mutation in CTCs based on a double spiral microfluidic chip and the real-time RPA method (2023-05-31)
期刊:ANALYTICAL AND BIOANALYTICAL CHEMISTRY
DOI:10.1007/s00216-023-04743-2
影响因子 :4.3
引用产品: HCC827 细胞
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Multi-omics analysis of expression profile and prognostic values of connexin family in LUAD (2023-07-17)
期刊:JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
DOI:10.1007/s00432-023-05075-5
影响因子 :3.6
引用产品: NCI-H1975 细胞 , HCC827 细胞 , A549 [A-549] 细胞 , PC-9 细胞 , BEAS-2B 细胞
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Circ_0006324 regulates cell proliferation, cell-cycle progression, apoptosis, and glycolysis of non-small cell lung cancer cells through miR-496/TRIM59 axis (2023-08-07)
期刊:JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
影响因子 :3.6
引用产品: A549 [A-549] 细胞 , NCI-H1975 细胞 , NCI-H522[H522] 细胞 , HCC827 细胞 , BEAS-2B 细胞
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IGF2BP1 enhances the stability of SIK2 mRNA through m6A modification to promote non-small cell lung cancer progression (2023-10-12)
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2023.10.045
影响因子 :3.1
引用产品: A549 [A-549] 细胞 , NCI-H1299 细胞 , HCC827 细胞 , NCI-H1975 细胞 , 人支气管上皮细胞
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Upregulation of NDUFAF2 in Lung Adenocarcinoma Is a Novel Independent Prognostic Biomarker (2023-01-17)
期刊:Computational and Mathematical Methods in Medicine
影响因子 :2.8
引用产品: PC-9 细胞 , NCI-H358 细胞 , A549 [A-549] 细胞 , HCC827 细胞 , NCI-H1299 细胞 , NCI-H1975 细胞 , BEAS-2B 细胞
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FATP2 regulates non-small cell lung cancer by mediating lipid metabolism through ACSL1 (2023-05-06)
期刊:TISSUE & CELL
DOI:10.1016/j.tice.2023.102105
影响因子 :2.6
引用产品: A549 [A-549] 细胞 , HCC827 细胞 , NCI-H1299 细胞 , NCI-H23 细胞 , BEAS-2B 细胞 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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Circ-NUP98 Promotes Lung Adenocarcinoma Development Through Regulating CBX1 by miR-188-3p (2023-12-21)
期刊:BIOCHEMICAL GENETICS
DOI:10.1007/s10528-023-10609-0
影响因子 :2.4
引用产品: BEAS-2B 细胞 , HCC827 细胞 , A549 [A-549] 细胞
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CircSFMBT2 Plays an Oncogenic Role in Lung Adenocarcinoma Depending on the miR-1305/SALL4 Axis (2023-12-21)
期刊:BIOCHEMICAL GENETICS
DOI:10.1007/s10528-023-10611-6
影响因子 :2.4
引用产品: BEAS-2B 细胞 , NCI-H1975 细胞 , HCC827 细胞 , A549 [A-549] 细胞 , RPMI-1640 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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RBM10 Is a Biomarker Associated with Pan-Cancer Prognosis and Immune Infiltration: System Analysis Combined with In Vitro and Vivo Experiments (2022-11-28)
期刊:Oxidative Medicine and Cellular Longevity
影响因子 :7.3
引用产品: HCC827 细胞
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Circular RNA lysophosphatidic acid receptor 3 (circ-LPAR3) enhances the cisplatin resistance of ovarian cancer (2001-02-20)
期刊:Bioengineered
DOI:10.1080/21655979.2022.2029109
影响因子 :4.9
引用产品: A2780 细胞 , SK-OV-3/DDP 细胞 , A2780/DDP 细胞 , SK-OV-3 细胞 , SK-OV-3细胞专用培养基 , SK-OV-3/DDP细胞专用培养基 , A2780细胞专用培养基 , A2780/DDP细胞专用培养基 , NCI-H1299 细胞 , A549 [A-549] 细胞 , HCC827 细胞 , NCI-H460 [H460] 细胞
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SLC7A5 is a lung adenocarcinoma-specific prognostic biomarker and participates in forming immunosuppressive tumor microenvironment (2022-10-02)
期刊:Heliyon
DOI:10.1016/j.heliyon.2022.e10866
影响因子 :3.8
引用产品: A549 [A-549] 细胞 , HCC827 细胞 , NCI-H1975 细胞 , PC-9 细胞
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Proteasome 26S subunit, non-ATPase 1 (PSMD1) facilitated the progression of lung adenocarcinoma by the de-ubiquitination and stability of PTEN-induced kinase 1 (PINK1) (2002-02-20)
期刊:Experimental Cell Research
DOI:10.1016/j.yexcr.2022.113075
影响因子 :3.7
引用产品: SK-MES-1 细胞 , NCI-H2170 细胞 , HCC827 细胞 , A549 [A-549] 细胞
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METTL3 promotes non-small cell lung cancer (NSCLC) cell proliferation and colony formation in a m6A-YTHDF1 dependent way (2022-08-25)
期刊:BMC Pulmonary Medicine
DOI:10.1186/s12890-022-02119-3
影响因子 :3.3
引用产品: A549 [A-549] 细胞 , NCI-H1299 细胞 , HCC827 细胞 , NCI-H1975 细胞 , 人支气管上皮细胞 , 青霉素-链霉素溶液 (双抗),100×
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Circular RNA Circ_0005962 Contributes to Lung Adenocarcinoma Cell Proliferation and Stem Cell Formation Through Sponging of miR-3611 and Modulating CYP24A1 Expression (2022-12-21)
期刊:BIOCHEMICAL GENETICS
DOI:10.1007/s10528-022-10312-6
影响因子 :2.2
引用产品: BEAS-2B 细胞 , A549 [A-549] 细胞 , HCC827 细胞 , NCI-H1975 细胞 , BEAS-2B细胞专用培养基 , DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , A549 [A-549]细胞专用培养基 , Ham's F-12K 培养基 , HCC827细胞专用培养基 , RPMI-1640 培养基 , NCI-H1975细胞专用培养基
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YBX1 regulated by Runx3-miR-148a-3p axis facilitates non-small-cell lung cancer progression (2005-01-20)
期刊:Cellular Signalling
DOI:10.1016/j.cellsig.2021.110049
影响因子 :4.8
引用产品: BEAS-2B , HCC827 细胞 , NCI-H1650 细胞 , NCI-H1299 细胞 , NCI-H358 细胞 , A549 [A-549] 细胞 , RPMI-1640 培养基 , 人支气管上皮细胞完全培养基
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Circ_0089823 reinforces malignant behaviors of non-small cell lung cancer by acting as a sponge for microRNAs targeting SOX4, (2007-01-20)
期刊:Neoplasia
影响因子 :4.8
引用产品: NCI-H1299 细胞 , HCC827 细胞 , NCI-H1437 细胞 , NCI-H446 [H446] 细胞 , A549 [A-549] 细胞 , Ham's F-12K 培养基
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CLEC12B suppresses lung cancer progression by inducing SHP-1 expression and inactivating the PI3K/AKT signaling pathway (2011-01-20)
期刊:Experimental Cell Research
DOI:10.1016/j.yexcr.2021.112914
影响因子 :3.7
引用产品: A549 [A-549] 细胞 , HCC827 细胞 , SK-MES-1 细胞
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Overexpression of Human Aspartyl (Asparaginyl) β-hydroxylase in NSCLC: Its Diagnostic Value by Means of Exosomes of Bronchoalveolar Lavage (2011-01-20)
期刊:Applied Immunohistochemistry & Molecular Morphology
DOI:10.1097/PAI.0000000000000963
影响因子 :1.6
引用产品: A549 [A-549] 细胞 , NCI-H1299 细胞 , NCI-H1650 细胞 , HCC827 细胞
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A two-circular RNA signature as a noninvasive diagnostic biomarker for lung adenocarcinoma (2002-09-20)
期刊:Journal of Translational Medicine
影响因子 :7.4
引用产品: NCI-H1299 细胞 , HCC827 细胞 , 16HBE 细胞
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Novel 4-anilinoquinazoline derivatives featuring an 1-adamantylmoiety as potent EGFR inhibitors with enhanced activity againstNSCLC cell lines (2001-06-20)
期刊:European Journal of Medicinal Chemistry
DOI:10.1016/j.ejmech.2016.01.045
影响因子 :6.7
引用产品: A-431 细胞 , HCC827 细胞 , NCI-H1975 细胞 , A549 [A-549] 细胞 , MCF7 [MCF-7] 细胞
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