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产品概述
| 名称 | 293T/17 (人胚肾细胞) (STR鉴定正确) |
| 别称 | HEK 293T/17; 293T/17 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 该细胞贴壁松散，操作时请尽量轻柔；换液时需预热培养基；收货如有大块脱落的细胞团，为正常现象，请按照收货注意事项处理。 |
| 背景描述 | 293T/17细胞株是293T细胞株的衍生株，293T是293 [HEK-293]细胞株插入了SV40 T-antigen的温度敏感基因形成的高转衍生株。293T细胞进行克隆，检测pBND和pZAP载体，得到一株能生成高滴度感染逆转录病毒的衍生株293T/17细胞。293T/17细胞持续表达猿病毒40(SV40)大T抗原，而因其高转染能力筛选到17号克隆。293T/17细胞用pCRIPenv-和pCRIPgag-2载体转染得到ANJOU65细胞株，ANJOU65细胞再用pCRIPgag-2和pGPT2E载体转染得到亲宅性外壳表达细胞株BOSC 23。ANJOU65细胞用携带gpt抗性基因的pCRIPAMgag载体转染得到Bing双向性表达包装细胞株。 |
| 年龄（性别） | 胚胎 |
| 组织来源 | 肾 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | BSL-2 |
| 保藏机构 | ATCC; CRL-11268 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11,12 D2S1338 19 D3S1358 15,16,17 D5S818 8,9 D7S820 11 D8S1179 11,12,14 D13S317 12,14 D16S539 9,13 D18S51 17,18 D19S433 18 D21S11 28,30.2 FGA 23 PentaD 9,10 PentaE 7,15 TH01 7,9.3 TPOX 11 vWA 16,19 D6S1043 11 D12S391 19,21 D2S441 11,15 -
STR鉴定图
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参考文献
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Genetically engineered CXCR4-modified exosomes for delivery of miR-126 mimics to macrophages alleviate periodontitis (2023-03-30)
作者：Haotian Luo:1.Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, 56 Lingyuanxi Road, Guangzhou, 510055, China. ; Danying Chen:1.Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, 56 Lingyuanxi Road, Guangzhou, 510055, China. ; Ruoyu Li:1.Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, 56 Lingyuanxi Road, Guangzhou, 510055, China. ; Runze Li:1.Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, 56 Lingyuanxi Road, Guangzhou, 510055, China. ; Yungshan Teng:1.Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, 56 Lingyuanxi Road, Guangzhou, 510055, China. ; Yang Cao:1.Hospital of
期刊：Genetically engineered CXCR4-modified exosomes for delivery of miR-126 mimics to macrophages alleviate periodontitis
DOI：10.1186/s12951-023-01863-w
影响因子 ：10.2
引用产品： 293T/17 细胞
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METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling. (2023-03-14)
作者：Shuhua Luo:1.Department of Anesthesiology, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524000, Guangdong, China; Guangdong Medical University, Zhanjiang 524000, Guangdong, China. ; Chaoxiong Liao:1.Department of Anesthesiology, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524000, Guangdong, China; Guangdong Medical University, Zhanjiang 524000, Guangdong, China. ; Lina Zhang:1.Department of Anesthesiology, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524000, Guangdong, China; Guangdong Medical University, Zhanjiang 524000, Guangdong, China. ; Chunxiu Ling:1.Department of Anesthesiology, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524000, Guangdong, China; Guangdong Medical University, Zhanjiang 524000, Guangdong, China. ; Xuedi Zhang:1.Department of Anesthesiology, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524000, Guangdong, China; Guangdong Medical University, Zhanjiang 524000, Guangdong
期刊：METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling.
DOI：10.1016/j.celrep.2023.112259
影响因子 ：8.8
引用产品： HL-60 细胞 , 293T/17 细胞
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Enhanced single RNA imaging reveals dynamic gene expression in live animals (2023-03-03)
作者：Yucen Hu:1.Zhejiang Provincial Key Laboratory of Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou, China. ; Jingxiu Xu:1.International Biomedicine-X research center of the Second Affiliated Hospital, Zhejiang University, Hangzhou, China. ; Erqing Gao:1.International Biomedicine-X research center of the Second Affiliated Hospital, Zhejiang University, Hangzhou, China. ; Xueyuan Fan:1.Zhejiang Provincial Key Laboratory of Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou, China. ; Jieli Wei:1.Zhejiang Provincial Key Laboratory of Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou, China. ; Bingcheng Ye:1.Zhejiang Provincial Key Laboratory of Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou, China. ; Suhong Xu:1.International Biomedicine-X research center of the Second Affiliated Hospital, Zhejiang University, Hangzhou, China. ; 2.Center fo
期刊：Enhanced single RNA imaging reveals dynamic gene expression in live animals
影响因子 ：7.7
引用产品： 293T/17 细胞 , NIH/3T3 细胞
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Differential expression profiling of tRNA-Derived small RNAs and their potential roles in methamphetamine self-administered rats (2023-02-02)
作者：Yun Zhou:1.School of Medicine, Ningbo University, Laboratory of Behavioral Neuroscience, Ningbo Kangning Hospital, Ningbo, Zhejiang, China. ; Qingxiao Hong:1.School of Medicine, Ningbo University, Laboratory of Behavioral Neuroscience, Ningbo Kangning Hospital, Ningbo, Zhejiang, China. ; 2.Key Laboratory of Addiction Research of Zhejiang Province, Ningbo, Zhejiang, China. ; Wenjin Xu:1.School of Medicine, Ningbo University, Laboratory of Behavioral Neuroscience, Ningbo Kangning Hospital, Ningbo, Zhejiang, China. ; 2.Key Laboratory of Addiction Research of Zhejiang Province, Ningbo, Zhejiang, China. ; Weisheng Chen:1.School of Medicine, Ningbo University, Laboratory of Behavioral Neuroscience, Ningbo Kangning Hospital, Ningbo, Zhejiang, China. ; 2.Key Laboratory of Addiction Research of Zhejiang Province, Ningbo, Zhejiang, China. ; Xiaohu Xie:1.School of Medicine, Ningbo University, Laboratory of Behavioral Neuroscience, Ningbo Kangning Hospital, Ningbo, Zhejiang, China. ; 2.Key Lab
期刊：Differential expression profiling of tRNA-Derived small RNAs and their potential roles in methamphetamine self-administered rats
DOI：10.3389/fgene.2023.1088498
影响因子 ：3.7
引用产品： 293T/17 细胞
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Warburg effect-promoted exosomal circ_0072083 releasing up-regulates NANGO expression through multiple pathways and enhances temozolomide resistance in glioma (2005-01-20)
作者：Chenyu Ding:1.Department of Neurosurgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350001, Fujian, People's Republic of China. ; 2.Fujian Provincial Key Laboratory of Precision Medicine for Cancer, Fuzhou, 350001, Fujian, People's Republic of China. ; Xuehan Yi:1.Department of Otolaryngology Head and Neck Surgery, Fujian Medical University Union Hospital, Fuzhou, 350001, Fujian, People's Republic of China. ; Xiangrong Chen:1.Department of Neurosurgery, The Second Affiliated Hospital, Fujian Medical University, Quanzhou, 362000, Fujian, People's Republic of China. ; Zanyi Wu:1.Department of Neurosurgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350001, Fujian, People's Republic of China. ; Honghai You:1.Department of Neurosurgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350001, Fujian, People's Republic of China. ; Xiaoyong Chen:1.Department of Neurosurgery, The First Affiliated Hospital of Fujian Med
期刊：Warburg effect-promoted exosomal circ_0072083 releasing up-regulates NANGO expression through multiple pathways and enhances temozolomide resistance in glioma
DOI：10.1186/s13046-021-01942-6
影响因子 ：11.3
引用产品： U251 细胞 , U-87 MG 细胞 , 293T/17 细胞
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