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产品概述
| 名称 | MeWo (人恶性黑色素瘤细胞) (STR鉴定正确) |
| 别称 | MEWO; Mewo; Me Wo; Me-Wo; Mevo; SK-MEL-MeWo; Mel-MeWo; BI-Mel; EST50 |
| 种属 | 人类 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:3 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | MeWo是从一名患有恶性黑色素瘤的78岁白人男性患者的皮肤中分离出来的一种具有成纤维细胞形态的细胞系。该产品在毒理学方面有应用。 |
| 年龄（性别） | 男性；78岁 |
| 组织来源 | 皮肤；恶性黑色素瘤 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 黑色素瘤细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~32 hours |
| 保藏机构 | ATCC; HTB-65 |
STR鉴定
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STR位点信息
Amelogenin X, Y CSF1PO 12 D2S1338 21,23 D3S1358 17 D5S818 12,13 D7S820 10,12 D8S1179 13,15 D13S317 8,9 D16S539 10,12 D18S51 14,17 D19S433 14,16 D21S11 30,32.2 FGA 22 PentaD 10 PentaE 5,15 TH01 7,9 TPOX 8,10 vWA 15 D6S1043 12 D12S391 17 D2S441 10,11 -
STR鉴定图
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参考文献
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LSM2 is associated with a poor prognosis and promotes cell proliferation, migration, and invasion in skin cutaneous melanoma (2023-06-13)
作者：Xiaofang Sun:1.Department of Dermatology, the Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China. ; 2.Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Soochow University, Soochow University, Jiangsu, China. ; Jianping Zhang:1.Department of Dermatology, the Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China. ; Jiayuan Hu:1.Department of Dermatology, the Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China. ; Qingdong Han:1.Department of Dermatology, the Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China. ; Zili Ge:1.Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Soochow University, Soochow University, Jiangsu, China. sdgzlfy@163.com. ;
期刊：LSM2 is associated with a poor prognosis and promotes cell proliferation, migration, and invasion in skin cutaneous melanoma
DOI：10.1186/s12920-023-01564-1
影响因子 ：2.7
引用产品： A2058 细胞 , A-375 细胞 , MeWo 细胞 , SK-MEL-2 细胞 , SK-MEL-28 细胞 , 人表皮黑色素细胞
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