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产品概述
氨基酸(Amino Acids)是蛋白质组成的基本单位,也是构成生命的重要成分。根据细胞自身合成的能力,可将氨基酸分为三类:必需氨基酸、半必需氨基酸和非必需氨基酸。MEM非必需氨基酸溶液(Non-Essential Amino Acids, NEAA)源自MEM培养基配方,包括L-丙氨酸、L-谷氨酸、L-天(门)冬酰胺、L-天(门)冬氨酸、L-脯氨酸、L-丝氨酸和甘氨酸7种非必需氨基酸,能有效改善细胞培养基配比,降低细胞培养时细胞自身生产非必需氨基酸的副作用,促进细胞增殖代谢,是细胞培养中常用的添加剂之一。
产品信息
形态 | 液体 |
浓度 | 100 × |
规格 | 100mL |
pH | 0.6-1.7 |
溶剂 | 纯化水 |
储存条件 | 2-8℃ |
运输条件 | 冰袋运输 |
有效期 | 12个月 |
注意事项
1、本产品经0.1 μm过滤除菌。
2、使用本产品时应注意无菌操作,避免污染。
3、本产品为100×浓缩液,请根据需要稀释后使用;
4、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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Acidosis activates breast cancer ferroptosis through ZFAND5/SLC3A2 signaling axis and elicits M1 macrophage polarization (2024-02-14)
期刊:CANCER LETTERS
DOI:10.1016/j.canlet.2024.216732
影响因子 :9.7
引用产品: McCoy's 5A 培养基 , DMEM高糖 培养基 , 马血清 , EGF/Urogastrone/URG, Human, Recombinant , 重组人胰岛素溶液 (10mg/mL) , MEM非必需氨基酸溶液 (NEAA),100× , 青霉素-链霉素溶液 (双抗),100× , RPMI-1640 培养基
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Role of macrophage AHR/TLR4/STAT3 signaling axis in the colitis induced by non-canonical AHR ligand aflatoxin B1 (2023-03-23)
期刊:JOURNAL OF HAZARDOUS MATERIALS
DOI:10.1016/j.jhazmat.2023.131262
影响因子 :13.6
引用产品: RPMI-1640 培养基 , L-谷氨酰胺溶液 (200mM),100× , β-巯基乙醇 (55mM),1000× , HEPES液 (1M) , MEM非必需氨基酸溶液 (NEAA),100×
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Characterization of Cancer Cell Mechanics by Measuring Active Deformation Behavior (2023-09-29)
期刊:Small Methods
影响因子 :12.4
引用产品: DMEM/F12 培养基 , 马血清 , EGF/Urogastrone/URG, Human, Recombinant , 重组人胰岛素溶液 (10mg/mL) , MEM非必需氨基酸溶液 (NEAA),100× , Hela 细胞 , MCF 10A 细胞
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DNA Nanoribbon-Assisted Intracellular Biosynthesis of Fluorescent Gold Nanoclusters for Cancer Cell Imaging (2023-09-06)
期刊:JACS Au
影响因子 :8.0
引用产品: DMEM/F12 培养基 , 重组人胰岛素溶液 (10mg/mL) , MEM非必需氨基酸溶液 (NEAA),100× , EGF/Urogastrone/URG, Human, Recombinant , 马血清 , MTT溶液 (5mg/mL) , MCF 10A 细胞
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Interferon-γ regulates immunosuppression in septic mice by promoting the Warburg effect through the PI3K/AKT/mTOR pathway (2023-07-11)
期刊:MOLECULAR MEDICINE
DOI:10.1186/s10020-023-00690-x
影响因子 :5.7
引用产品: DC2.4 细胞 , L-谷氨酰胺溶液 (200mM),100× , 青霉素-链霉素溶液 (双抗),100× , MEM非必需氨基酸溶液 (NEAA),100× , β-巯基乙醇 (55mM),1000× , 特级胎牛血清
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Molecular mechanism of Gan-song Yin inhibiting the proliferation of renal tubular epithelial cells by regulating miR-21-5p in adipocyte exosomes (2023-12-01)
期刊:JOURNAL OF ETHNOPHARMACOLOGY
影响因子 :5.4
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CXCL8 Promotes Endothelial-to-Mesenchymal Transition of Endothelial Cells and Protects Cells from Erastin-Induced Ferroptosis via CXCR2-Mediated Activation of the NF-κB Signaling Pathway (2023-08-25)
期刊:Pharmaceuticals
影响因子 :4.6
引用产品: PUMC-HUVEC-T1 细胞 , THP-1 细胞 , DMEM高糖 培养基 , MEM非必需氨基酸溶液 (NEAA),100× , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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Downregulation of the long noncoding RNA DSCR9 (Down syndrome critical region 9) delays breast cancer progression by modulating microRNA-504-5p-dependent G protein-coupled receptor 65 (2023-05-29)
期刊:Human Cell
DOI:10.1007/s13577-023-00916-4
影响因子 :4.3
引用产品: MCF 10A 细胞 , DMEM/F12 (含L-丙氨酰-L-谷氨酰胺、HEPES) 培养基 , 马血清 , MEM非必需氨基酸溶液 (NEAA),100× , 青霉素-链霉素溶液 (双抗),100× , DMEM高糖 培养基 , 特级胎牛血清 , RPMI-1640 培养基 , 293T [HEK-293T] 细胞
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Z-Guggulsterone Induces Cell Cycle Arrest and Apoptosis by Targeting the p53/CCNB1/PLK1 Pathway in Triple-Negative Breast Cancer (2023-01-03)
期刊:ACS Omega
影响因子 :4.1
引用产品: DMEM/F12 培养基 , 马血清 , EGF/Urogastrone/URG, Human, Recombinant , 重组人胰岛素溶液 (10mg/mL) , MEM非必需氨基酸溶液 (NEAA),100×
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E2F1-mediated ectopic expression of PP1A promotes breast cancer progression via activation of YAP1 (2023-02-12)
期刊:INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
DOI:10.1016/j.biocel.2023.106389
影响因子 :4.0
引用产品: DMEM/F12 培养基 , 马血清 , MEM非必需氨基酸溶液 (NEAA),100× , 青霉素-链霉素溶液 (双抗),100× , MEM (含NEAA) 培养基 , 特级胎牛血清 , RPMI-1640 培养基 , L-谷氨酰胺溶液 (200mM),100× , DMEM高糖 培养基
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The HDAC inhibitor HFY-4A improves TUSC2 transcription to induce immunogenic cell death in breast cancer (2023-09-22)
期刊:TOXICOLOGY AND APPLIED PHARMACOLOGY
DOI:10.1016/j.taap.2023.116698
影响因子 :3.8
引用产品: MCF7 [MCF-7] 细胞 , T-47D 细胞 , MCF 10A 细胞 , DMEM高糖 培养基 , RPMI-1640 培养基 , DMEM/F12 培养基 , MEM非必需氨基酸溶液 (NEAA),100×
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Cytochrome b561 regulates iron metabolism by activating the Akt/mTOR pathway to promote Breast Cancer Cells proliferation (2023-08-26)
期刊:EXPERIMENTAL CELL RESEARCH
DOI:10.1016/j.yexcr.2023.113760
影响因子 :3.7
引用产品: DMEM高糖 培养基 , MEM非必需氨基酸溶液 (NEAA),100× , 青霉素-链霉素溶液 (双抗),100× , 马血清 , 磷酸盐缓冲液 (PBS) 1×
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Nitidine chloride regulates cell function of bladder cancer in vitro through downregulating Lymphocyte antigen 75 (2023-03-14)
期刊:NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
DOI:10.1007/s00210-023-02446-0
影响因子 :3.6
引用产品: UM-UC-3 细胞 , MEM非必需氨基酸溶液 (NEAA),100× , Annexin V-APC/ PI荧光双染细胞凋亡检测试剂盒
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Circ_0026579 knockdown ameliorates lipopolysaccharide-induced human lung fibroblast cell injury by regulating CXCR1 via miR-370-3p (2023-10-02)
期刊:CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
影响因子 :2.9
引用产品: MRC-5 细胞 , MEM 培养基 , 特级胎牛血清 , MEM非必需氨基酸溶液 (NEAA),100× , 青霉素-链霉素溶液 (双抗),100×
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Identification of TFR2 as a novel ferroptosis‑related gene that serves an important role in prognosis and progression of triple‑negative breast cancer (2023-12-05)
期刊:Oncology Letters
影响因子 :2.9
引用产品: MCF 10A 细胞 , T-47D 细胞 , SK-BR-3 [SKBR3] 细胞 , BT-549 细胞 , MDA-MB-468 细胞 , MDA-MB-436 细胞 , MDA-MB-231 细胞 , 马血清 , MEM非必需氨基酸溶液 (NEAA),100×
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Inhibition of glycolysis represses the growth and alleviates the endoplasmic reticulum stress of breast cancer cells by regulating TMTC3 (2023-04-10)
期刊:Open Medicine
影响因子 :2.1
引用产品: MCF 10A 细胞 , MCF7 [MCF-7] 细胞 , MDA-MB-231 细胞 , MDA-MB-436 细胞 , SK-BR-3 [SKBR3] 细胞 , DMEM/F12 培养基 , 马血清 , MEM非必需氨基酸溶液 (NEAA),100× , 青霉素-链霉素溶液 (双抗),100× , RPMI-1640 (ATCC改良) 培养基 , Leibovitz's L-15 培养基 , McCoy's 5A 培养基 , 特级胎牛血清
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Three-dimensional layered nanofiber sponge with in situ grown silver- metal organic framework for enhancing wound healing (2004-02-20)
期刊:Chemical Engineering Journal
影响因子 :15.1
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Regulation of Atherosclerosis by Toll-Like Receptor 4 Induced by Serum Amyloid 1: A Systematic In Vitro Study (2022-09-15)
期刊:Biomed Research International
影响因子 :3.2
引用产品: 人脐静脉内皮细胞 , MEM非必需氨基酸溶液 (NEAA),100× , 重组人胰岛素溶液 (10mg/mL) , THP-1 细胞
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LncRNA SNHG3 regulates the BMSC osteogenic differentiation in bone metastasis of breast cancer by modulating the miR-1273g-3p/BMP3 axis (2001-02-20)
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2021.12.075
影响因子 :3.1
引用产品: DMEM/F12 培养基 , MEM非必需氨基酸溶液 (NEAA),100×
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MicroRNA-377-3p Promotes Cell Proliferation and Inhibits Cell Cycle Arrest and Cell Apoptosis in Hepatocellular Carcinoma by Affecting EGR1-mediated p53 Activation (2003-02-20)
期刊:Pathology Research And Practice
影响因子 :2.8
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Macroscopic Supramolecular Assembly Strategy to Construct 3D Biocompatible Microenvironments with Site-Selective Cell Adhesion (2006-01-20)
期刊:ACS Applied Materials & Interfaces
影响因子 :9.5
引用产品: MG-63 细胞 , PC-12 (Undifferentiated) 细胞 , 重组人胰岛素溶液 (10mg/mL) , MEM非必需氨基酸溶液 (NEAA),100× , PUMC-HUVEC-T1 , CCC-SMC-1 细胞
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N-2-(phenylamino) benzamide derivatives as novel anti-glioblastoma agents: Synthesis and biological evaluation (2009-01-20)
期刊:European Journal Of Medicinal Chemistry
DOI:10.1016/j.ejmech.2021.113817
影响因子 :6.7
引用产品: U-87 MG 细胞 , U251 细胞 , C6 细胞 , MEM非必需氨基酸溶液 (NEAA),100×
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A nanosensor for precise discrimination of nephrotoxic drug mechanisms via dynamic fluorescence fingerprint strategy (2003-01-20)
期刊:Analytica Chimica Acta
影响因子 :6.2
引用产品: MEM非必需氨基酸溶液 (NEAA),100× , HK-2 细胞
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