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产品概述
Dulbecco's 改良培养基--DMEM(Dulbecco's Modified Eagle Medium)是在MEM培养基的基础上研制的,与MEM培养基相比,氨基酸的含量增加了2倍,维生素增加了4倍,同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。DMEM培养基最初设计葡萄糖含量为1000 mg/L(低糖型),后来又发展出葡萄糖含量为4500 mg/L(高糖型),现已广泛应用于各种细胞的培养。DMEM低糖培养基更适合代谢作用较慢、依赖性贴壁细胞的培养。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分,但不含蛋白质、脂类或任何生长因子,故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
L-谷氨酰胺 | 4 mM |
NaHCO3 | 3700 mg/L |
D-葡萄糖 | 1000 mg/L |
丙酮酸钠 | 1 mM |
HEPES缓冲剂 | 无 |
酚红指示剂 | 15 mg/L |
储存条件 | 2-8℃,避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌,使用时应注意无菌操作,避免污染;
2、为保持本产品的最佳使用效果,请勿进行冻融处理;
3、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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N6-methyladenosine in 28S rRNA promotes oncogenic mRNA translation and tyrosine catabolism (2024-12-30)
期刊:Cell Reports
DOI:10.1016/j.celrep.2024.115139
影响因子 :7.5
引用产品: DMEM低糖 培养基 , 青霉素-链霉素-两性霉素B溶液(100×)
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Upregulation of rate-limiting enzymes in cholesterol metabolism by PKCδ mediates endothelial apoptosis in diabetic wound healing (2024-05-29)
期刊:Cell Death Discovery
DOI:10.1038/s41420-024-02030-2
影响因子 :7.0
引用产品: DMEM高糖 培养基 , DMEM低糖 培养基 , 特级胎牛血清
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GLP-1 analogue liraglutide attenuates CIH-induced cognitive deficits by inhibiting oxidative stress, neuroinflammation, and apoptosis via the Nrf2/HO-1 and MAPK/NF-κB signaling pathways (2024-09-24)
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2024.113222
影响因子 :4.8
引用产品: 青霉素-链霉素溶液(双抗),100× , DMEM低糖 培养基 , SH-SY5Y [SHSY-5Y] 细胞 , 0.25%胰蛋白酶溶液(含EDTA,溶于PBS) , 特级胎牛血清
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The mechanisms and therapeutic potential of clopidogrel in mitigating diabetic cardiomyopathy in db/db mice (2024-02-05)
期刊:iScience
DOI:10.1016/j.isci.2024.109134
影响因子 :4.6
引用产品: DMEM低糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液(双抗),100× , D-葡萄糖溶液(200 g/L)
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Txnip promotes autophagic apoptosis in diabetic cardiomyopathy by upregulating FoxO1 and its acetylation (2024-10-11)
期刊:CELLULAR SIGNALLING
DOI:10.1016/j.cellsig.2024.111469
影响因子 :4.4
引用产品: DMEM低糖 培养基 , 青霉素-链霉素溶液(双抗),100× , 特级胎牛血清
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Interactions of oleanane pentacyclic triterpenoids with human organic anion transporting polypeptide 1B1 and 1B3 (2024-05-16)
期刊:TOXICOLOGY IN VITRO
影响因子 :3.2
引用产品: DMEM低糖 培养基
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Jagged1-Notch1 Signaling Pathway Induces M1 Microglia to Disrupt the Barrier Function of Retinal Microvascular Endothelial Cells (2024-05-23)
期刊:CURRENT EYE RESEARCH
DOI:10.1080/02713683.2024.2357601
影响因子 :2.0
引用产品: HMC3 细胞 , DMEM低糖 培养基 , HMC3细胞专用培养基
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Sirtuin1 Mediates the Protective Effects of Echinacoside against Sepsis-Induced Acute Lung Injury via Regulating the NOX4-Nrf2 Axis (2023-10-29)
期刊:Antioxidants
影响因子 :7.0
引用产品: DMEM低糖 培养基
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TIFA contributes to periodontitis in diabetic mice via activating the NF?κB signaling pathway (2023-12-13)
期刊:Molecular Medicine Reports
影响因子 :3.4
引用产品: DMEM低糖 培养基
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The New Role of HNF1A-NAS1/miR-214/INHBA Signaling Axis in Colorectal Cancer (2023-11-24)
期刊:Frontiers in Bioscience-Landmark
影响因子 :3.1
引用产品: DMEM高糖 培养基 , Leibovitz's L-15 培养基 , Ham's F-12K 培养基 , 特级胎牛血清 , Annexin V-FITC染色液 , DMEM高糖 培养基
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Circ_0005736 promotes tenogenic differentiation of tendon-derived stem cells through the miR-636/MAPK1 axis (2023-09-05)
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-023-04115-7
影响因子 :2.6
引用产品: DMEM低糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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S-Equol enhances osteoblastic bone formation and prevents bone loss through OPG/RANKL via the PI3K/Akt pathway in streptozotocin-induced diabetic rats (2022-10-21)
期刊:Frontiers in Nutrition
影响因子 :6.6
引用产品: DMEM低糖 培养基 , ROS17/2.8 细胞
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Facile Amidogen Bio-Activation Method Can Boost the Soft Tissue Integration on 3D Printed Poly–Ether–Ether–Ketone Interface (2021-09-09)
期刊:Advanced Materials Interfaces
影响因子 :5.4
引用产品: DMEM低糖 培养基 , 磷酸盐缓冲液 (PBS) 1×
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Mangiferin inhibits cell migration and angiogenesis via PI3K/AKT/mTOR signaling in high glucose? and hypoxia?induced RRCECs (2021-04-21)
期刊:Molecular Medicine Reports
影响因子 :3.4
引用产品: DMEM低糖 培养基 , 大鼠视网膜微血管内皮细胞
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Resveratrol prevents inflammation and oxidative stress response in LPS-induced human gingival fibroblasts by targeting the PI3K/AKT and Wnt/β-catenin signaling pathways (2021-07-02)
期刊:GENETICS AND MOLECULAR BIOLOGY
DOI:10.1590/1678-4685-GMB-2020-0349
影响因子 :1.8
引用产品: DMEM低糖 培养基
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