产品中心Cell Resources
联系我们CONTACT US
- 400-999-210024小时服务热线
产品概述
Dulbecco's 改良培养基--DMEM(Dulbecco's Modified Eagle Medium)是在MEM培养基的基础上研制的,与MEM培养基相比,氨基酸的含量增加了2倍,维生素增加了4倍,同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。DMEM培养基最初设计葡萄糖含量为1000 mg/L(低糖型),后来又发展出葡萄糖含量为4500 mg/L(高糖型),现已广泛应用于各种细胞的培养。DMEM低糖培养基更适合代谢作用较慢、依赖性贴壁细胞的培养。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分,但不含蛋白质、脂类或任何生长因子,故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
L-谷氨酰胺 | 4 mM |
NaHCO3 | 3700 mg/L |
D-葡萄糖 | 1000 mg/L |
丙酮酸钠 | 1 mM |
HEPES缓冲剂 | 无 |
酚红指示剂 | 15 mg/L |
储存条件 | 2-8℃,避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌,使用时应注意无菌操作,避免污染;
2、为保持本产品的最佳使用效果,请勿进行冻融处理;
3、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
-
Sirtuin1 Mediates the Protective Effects of Echinacoside against Sepsis-Induced Acute Lung Injury via Regulating the NOX4-Nrf2 Axis (2023-10-29)
作者:Weixi Xie:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Lang Deng:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Miao Lin:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Xiaoting Huang:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Rui Qian:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Dayan Xiong:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Wei Liu:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Siyuan Tang:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ;
期刊:Sirtuin1 Mediates the Protective Effects of Echinacoside against Sepsis-Induced Acute Lung Injury via Regulating the NOX4-Nrf2 Axis
影响因子 :7.0
引用产品: DMEM低糖 培养基
-
TIFA contributes to periodontitis in diabetic mice via activating the NF‑κB signaling pathway (2023-12-13)
作者:Xiaoqian Guo:1.Department of Periodontology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China. ; Guangwei Qiao:1.Department of Oral and Maxillofacial Surgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China. ; Jingjiao Wang:1.Department of Periodontology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China. ; Changyi Yang:1.Department of Periodontology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China. ; Min Zhao:1.Department of Periodontology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China. ; Qian Zhang:1.Department of Periodontology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China. ; Yingbiao Wan:1.Department of Prosthodontics and Oral Implantology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China. ;
期刊:TIFA contributes to periodontitis in diabetic mice via activating the NF‑κB signaling pathway
影响因子 :3.4
引用产品: DMEM低糖 培养基
-
The New Role of HNF1A-NAS1/miR-214/INHBA Signaling Axis in Colorectal Cancer (2023-11-24)
作者:Xuan Zhang:1.Department of Colorectal Surgery, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, 650118 Kunming, Yunnan, China. ; Tao Wu:1.Department of Colorectal Surgery, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, 650118 Kunming, Yunnan, China. ; Rujia Qin:1.Department of Head and Neck Tumor Surgery, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, 650118 Kunming, Yunnan, China. ; Xinyi Cai:1.Department of Colorectal Surgery, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, 650118 Kunming, Yunnan, China. ; Yongchun Zhou:1.Laboratory of Molecular Diagnosis Center, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, 650118 Kunming, Yunnan, China. ; Xiaoxiong Wang:1.Laboratory of Molecular Diagnosis Center, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, 650118 Kunmi
期刊:The New Role of HNF1A-NAS1/miR-214/INHBA Signaling Axis in Colorectal Cancer
影响因子 :3.1
引用产品: DMEM高糖 培养基 , Leibovitz's L-15 培养基 , Ham's F-12K 培养基 , 特级胎牛血清 , Annexin V-FITC染色液 , DMEM高糖 培养基
-
Circ_0005736 promotes tenogenic differentiation of tendon-derived stem cells through the miR-636/MAPK1 axis (2023-09-05)
作者:Guangzhao Yang:1.Department of Sports, Communication University of China, Nanjing, No.26, Pengshan Road, Jiangning District, Nanjing, 211172, China. yanggz@cucn.edu.cn. ; Fei Chen:1.Department of Sports, Communication University of China, Nanjing, No.26, Pengshan Road, Jiangning District, Nanjing, 211172, China. ; Chunyan Zhang:1.Department of Sports, Communication University of China, Nanjing, No.26, Pengshan Road, Jiangning District, Nanjing, 211172, China. ; Chenlin Gu:1.Faculty of Cultural Management, Communication University of China, Nanjing, Nanjing, China. ;
期刊:Circ_0005736 promotes tenogenic differentiation of tendon-derived stem cells through the miR-636/MAPK1 axis
DOI:10.1186/s13018-023-04115-7
影响因子 :2.6
引用产品: DMEM低糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
-
Facile Amidogen Bio-Activation Method Can Boost the Soft Tissue Integration on 3D Printed Poly–Ether–Ether–Ketone Interface (2009-01-20)
作者:Xi Liu: 1.Department of Thoracic Surgery, the Second Affiliated Hospital of The Air Force Military Medical University, Xi'an, Shaanxi, 710038 P. R. China; Lijun Huang: 1.Department of Thoracic Surgery, the Second Affiliated Hospital of The Air Force Military Medical University, Xi'an, Shaanxi, 710038 P. R. China; Hao Zhang: 1.Department of Thoracic Surgery, the Second Affiliated Hospital of The Air Force Military Medical University, Xi'an, Shaanxi, 710038 P. R. China; Yujian Liu: 1.Department of Thoracic Surgery, the Second Affiliated Hospital of The Air Force Military Medical University, Xi'an, Shaanxi, 710038 P. R. China; Chunyan Wu: 1.School of Advanced Materials and Nanotechnology, Interdisciplinary Research Center of Smart Sensing, Xidian University, Xi'an, Shaanxi, 710126 P. R. China; Qixing Luo: 1.School of Advanced Materials and Nanotechnology, Interdisciplinary Research Center of Smart Sensing, Xidian University, Xi'an, Shaanxi, 710126 P. R. China; Feiyang Yin: 1.Department of
期刊:Facile Amidogen Bio-Activation Method Can Boost the Soft Tissue Integration on 3D Printed Poly–Ether–Ether–Ketone Interface
影响因子 :5.4
引用产品: DMEM低糖 培养基 , 磷酸盐缓冲液 (PBS) 1×
-
Mangiferin inhibits cell migration and angiogenesis via PI3K/AKT/mTOR signaling in high glucose‑ and hypoxia‑induced RRCECs (2004-01-20)
作者:Jia Shi:1.Department of Ophthalmology, The People's Hospital of Deyang City, Deyang, Sichuan 618000, P.R. China. ; Hongbin Lv:1.Department of Ophthalmology, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China. ; Chen Tang:1.Department of Ophthalmology, The People's Hospital of Deyang City, Deyang, Sichuan 618000, P.R. China. ; Yujie Li:1.Department of Ophthalmology, The People's Hospital of Deyang City, Deyang, Sichuan 618000, P.R. China. ; Jing Huang:1.Department of Ophthalmology, The People's Hospital of Deyang City, Deyang, Sichuan 618000, P.R. China. ; Hong Zhang:1.Department of Ophthalmology, The People's Hospital of Deyang City, Deyang, Sichuan 618000, P.R. China. ;
期刊:Mangiferin inhibits cell migration and angiogenesis via PI3K/AKT/mTOR signaling in high glucose‑ and hypoxia‑induced RRCECs
影响因子 :3.4
引用产品: DMEM低糖 培养基 , 大鼠视网膜微血管内皮细胞
FAQs
Q:{{item.question}}
A:
产品资料
识别码示意图