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产品概述
胰蛋白酶（Trypsin）是一种丝氨酸水解酶，它能把多肽链中赖氨酸和精氨酸残基中的羧基侧切段，水解细胞间的蛋白质，破坏细胞间的连接，从而使组织或贴壁细胞离散成单个细胞。胰酶分散细胞的活性与组织或细胞的特性、胰酶浓度、温度和作用时间有关，在PH 8.0和37℃时，胰酶的作用能力最强，因此使用胰酶时，应把握好浓度、温度和时间，以免消化过度造成细胞损伤。
一般常用胰酶的工作浓度为0.25%，而半贴壁细胞或对胰酶敏感的细胞常采用低浓度（0.05%）的胰酶进行细胞消化。由于EDTA能够螯合Ca2+和Mg2+，从而破坏细胞连接促进细胞的解离，因此在胰酶溶液中常常会加入一定量的EDTA混合使用，以增强解离效果。
产品信息
形态 | 液体 |
规格 | 125mL/500mL |
溶剂 | 10mM PBS |
胰蛋白酶浓度 | 2.5g/L |
EDTA•2Na | 0.2g/L |
酚红指示剂 | 无 |
储存条件 | -5~-20℃ |
运输条件 | 低温 |
有效期 | 12个月 |
注意事项
1、由于不同的组织或者细胞对胰酶的作用反应不一样，因此操作人员应根据实际情况，确定最佳消化时间；消化细胞时间不宜过长，否则会影响细胞贴壁和生长状况；
2、使用本产品时应注意无菌操作，避免污染；
3、不宜长时间放置于室温环境或4℃长期保存；
4、2-8℃中解冻，摇匀后使用，切忌反复冻融，用量较少时建议分装冻存。
5、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
产品手册
参考文献
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期刊：International Journal of Surgery
DOI：10.1097/JS9.0000000000001402
影响因子 ：15.3
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期刊：Advanced Healthcare Materials
影响因子 ：10.0
引用产品： DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100× , 0.25%胰蛋白酶溶液 (含EDTA，溶于PBS)
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Angiotensin II type-2 receptor signaling facilitates liver injury repair and regeneration via inactivation of Hippo pathway (2024-03-15)
期刊：ACTA PHARMACOLOGICA SINICA
DOI：10.1038/s41401-024-01249-0
影响因子 ：8.2
引用产品： DMEM高糖 培养基 , DMEM/F12 培养基 , 0.25%胰蛋白酶溶液 (含EDTA，溶于PBS)
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Songorine modulates macrophage polarization and metabolic reprogramming to alleviate inflammation in osteoarthritis (2024-02-13)
期刊：Frontiers in Immunology
DOI：10.3389/fimmu.2024.1344949
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DOI：10.1186/s40538-024-00539-3
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期刊：COLLOIDS AND SURFACES B-BIOINTERFACES
DOI：10.1016/j.colsurfb.2024.113857
影响因子 ：5.8
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DOI：10.1016/j.ceramint.2024.02.041
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期刊：ADVANCED FUNCTIONAL MATERIALS
影响因子 ：19.0
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期刊：CHEMICAL ENGINEERING JOURNAL
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引用产品： DMEM高糖 培养基 , 0.25%胰蛋白酶溶液 (含EDTA，溶于PBS) , 青霉素-链霉素溶液 (双抗)，100×
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期刊：JOURNAL OF COLLOID AND INTERFACE SCIENCE
DOI：10.1016/j.jcis.2023.03.172
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引用产品： DMEM高糖 培养基 , 磷酸盐缓冲液 (PBS) 1× , 特级胎牛血清 , 0.25%胰蛋白酶溶液 (含EDTA，溶于PBS)
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期刊：SCIENCE OF THE TOTAL ENVIRONMENT
DOI：10.1016/j.scitotenv.2023.168446
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DOI：10.1016/j.scitotenv.2023.169140
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引用产品： THP-1 细胞 , 0.25%胰蛋白酶溶液 (含EDTA，溶于PBS)
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DOI：10.1021/acs.chemmater.3c01637
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DOI：10.1186/s13287-023-03505-2
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期刊：Journal of Animal Science and Biotechnology
DOI：10.1186/s40104-023-00903-7
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引用产品： DMEM/F12 培养基 , RPMI-1640 培养基 , 0.25%胰蛋白酶溶液 (含EDTA，溶于PBS)
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DOI：10.1016/j.eurpolymj.2023.112486
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DOI：10.3389/fbioe.2023.1153196
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引用产品： DMEM高糖 培养基 , 特级胎牛血清 , 0.25%胰蛋白酶溶液 (含EDTA，溶于PBS) , 磷酸盐缓冲液 (PBS) 1× , Annexin V-FITC/ PI荧光双染细胞凋亡检测试剂盒
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引用产品： DMEM高糖 培养基 , 青霉素-链霉素溶液 (双抗)，100× , 0.25%胰蛋白酶溶液 (含EDTA，溶于PBS)
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引用产品： DMEM高糖 培养基 , DMEM/F12 培养基 , 0.25%胰蛋白酶溶液 (含EDTA，溶于PBS)
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Network pharmacology and experimental validation of Maxing Shigan decoction in the treatment of influenza virus-induced ferroptosis (2023-10-23)
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DOI：10.3390/fermentation9050432
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期刊：Nutrients
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期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2022.109561
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期刊：JOURNAL OF DRUG TARGETING
DOI：10.1080/1061186X.2022.2142595
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Mechanical Properties and In Vitro Biocompatibility of Hybrid Polymer-HA/BAG Ceramic Dental Materials (2022-09-09)
期刊：Polymers
影响因子 ：5.0
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Potentilla bifurca flavonoids effectively improve insulin resistance (2022-11-01)
期刊：European Review for Medical and Pharmacological Sciences
DOI：10.26355/eurrev_202211_30370
影响因子 ：3.8
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Effects of soil-extractable metals Cd and Ni from an e-waste dismantling site on human colonic epithelial cells Caco-2: Mechanisms and implications (2012-01-20)
期刊：Chemosphere
DOI：10.1016/j.chemosphere.2021.133361
影响因子 ：8.9
引用产品： MEM 培养基 , 特级胎牛血清 , 0.25%胰蛋白酶溶液 (含EDTA，溶于PBS)
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期刊：International Journal Of Pharmaceutics
DOI：10.1016/j.ijpharm.2021.121083
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Effect of Trimethyltin chloride on proliferation and cell cycle of intestinal porcine epithelial cells (2007-01-20)
期刊：Comparative Biochemistry And Physiology C-Toxicology & Pharmacology
DOI：10.1016/j.cbpc.2021.109131
影响因子 ：4.5
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Downregulation of microRNA‐124 prevents the development of acute liver failure through the upregulation of PIM‐3 (2001-00-20)
期刊：Experimental Physiology
DOI：10.1113/EP087963
影响因子 ：2.7
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A novel pH-sensitive targeting polysaccharide-gold nanorod conjugate for combined photothermal-chemotherapy of breast cancer (2002-09-20)
期刊：Carbohydrate Polymers
DOI：10.1016/j.carbpol.2019.02.045
影响因子 ：11.2
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NIR/pH dual-responsive polysaccharide-encapsulated gold nanorods for enhanced chemo-photothermal therapy of breast cancer (2006-09-20)
期刊：Materials Science & Engineering C-Materials for Biological Applications
DOI：10.1016/j.msec.2019.109854
影响因子 ：7.9
引用产品： DMEM高糖 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100× , 0.25%胰蛋白酶溶液 (含EDTA，溶于PBS)
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Fumonisin B1 damages the barrier functions of porcine intestinal epithelial cells in vitro (2009-09-20)
期刊：Journal Of Biochemical And Molecular Toxicology
影响因子 ：3.6
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PEGylated hydrazided gold nanorods for pH-triggered chemo/photodynamic/photothermal triple therapy of breast cancer (2010-08-20)
期刊：Acta Biomaterialia
DOI：10.1016/j.actbio.2018.10.019
影响因子 ：9.3
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A dual-targeted hyaluronic acid-gold nanorod platform with triple-stimuli responsiveness for photodynamic/photothermal therapy of breast cancer (2011-08-20)
期刊：Acta Biomaterialia
DOI：10.1016/j.actbio.2018.11.026
影响因子 ：9.3
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TGF‑β/Smad3 pathway enhances the cardio‑protection of S1R/SIPR1 in in vitro ischemia‑reperfusion myocardial cell model (2007-08-20)
期刊：Experimental and Therapeutic Medicine
影响因子 ：2.7
引用产品： D-Hank's平衡盐溶液 (D-HBSS)，不含钙、镁、酚红 , 0.25%胰蛋白酶溶液 (含EDTA，溶于PBS)
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A double-network poly(Nɛ-acryloyl l-lysine)/hyaluronic acid hydrogel as a mimic of the breast tumor microenvironment (2001-06-20)
期刊：Acta Biomaterialia
DOI：10.1016/j.actbio.2016.01.027
影响因子 ：9.3
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