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人胃黏膜上皮细胞
Human Gastric Mucosal Epithelial Cells
货号：CP-H048
规格：5×10⁵Cells/T25培养瓶
价格： ￥6380
培养基货号： CM-H048
产品概述
人胃黏膜上皮细胞
Cat NO.: CP-H048
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产品名称：人胃黏膜上皮细胞
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组织来源：胃组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人胃黏膜上皮细胞分离自胃黏膜组织；胃黏膜柔软，活体呈橘红色。胃空虚时形成许多皱襞，充盈时变平坦。幽门处的黏膜形成环形皱襞，突向腔内称幽门瓣。胃黏膜可分为三层，即上皮层(单层柱状上皮)、固有层(由结缔组织构成、含有大量的胃腺)和黏膜肌层(为薄层平滑肌、排列成内环外纵)。胃黏膜上皮细胞源自胃黏膜的上皮层，细胞为单层柱状上皮，排列整齐，能分泌黏液覆盖于胃黏膜的表面，防止胃酸和胃蛋白酶对胃黏膜的损害。体外培养胃黏膜上皮细胞为研究细胞功能及致病因子、药物、激素对细胞的损伤、保护和功能调控提供了简单而优越的模型。
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方法简介：
普诺赛实验室分离的人胃黏膜上皮细胞采用胰蛋白酶-胶原酶混合消化法结合差速贴壁法，并通过上皮细胞专用培养基培养筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人胃黏膜上皮细胞经Cytokeratin-18免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 鼠尾胶原Ⅰ(2-5μg/cm2) 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H048 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 上皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 人胃黏膜上皮细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Homeobox Protein BarH-like 1 Promotes Gastric Cancer Progression by Activating Coiled-Coil Domain-Containing Protein 178 (2024-02-15)
作者：Yue Gu:1.Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang District, 150001, Harbin, Heilongjiang, People's Republic of China. 840324@hrbmu.edu.cn. ; Gang Chen:1.Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang District, 150001, Harbin, Heilongjiang, People's Republic of China. ; Xinwei Ning:1.Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang District, 150001, Harbin, Heilongjiang, People's Republic of China. ;
期刊：Homeobox Protein BarH-like 1 Promotes Gastric Cancer Progression by Activating Coiled-Coil Domain-Containing Protein 178
DOI：10.1007/s10620-024-08312-0
影响因子 ：3.1
引用产品： 人胃黏膜上皮细胞 , MKN-45 细胞 , AGS 细胞 , NCI-N87 [N87] 细胞 , KATO III [KATO 3] 细胞 , THP-1 细胞
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Amentoflavone attenuates cell proliferation and induces ferroptosis in human gastric cancer by miR-496/ATF2 axis (2023-07-16)
作者：Fengying Tang:1.Department of Integrated Chinese and Western Medicine, Clinical major of Integrated Chinese and Western Medicine, First Clinical Medical College, Shaanxi University of Chinese Medicine, Xianyang, China. ; Yongpan Xu:1.Department of Integrated Chinese and Western Medicine, Clinical major of Integrated Chinese and Western Medicine, First Clinical Medical College, Shaanxi University of Chinese Medicine, Xianyang, China. ; Erpeng Gao:1.Gastroenterology Department, The Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, China. ; Wei Zhang:1.Gastroenterology Department, The Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, China. ; Fengli Zhang:1.Gastroenterology Department, The Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, China. ; Yi Xiang:1.Gastroenterology Department, The Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, China. ; Lixiaoyuan Xu:1.
期刊：Amentoflavone attenuates cell proliferation and induces ferroptosis in human gastric cancer by miR-496/ATF2 axis
影响因子 ：3.0
引用产品： 人胃黏膜上皮细胞 , AGS 细胞 , HGC-27 细胞 , RPMI-1640 培养基
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Chemical Constituents and Anti-Gastric Ulcer Activity of Essential Oils of Alpinia officinarum (Zingiberaceae), Cyperus rotundus (Cyperaceae), and Their Herbal Pair (2008-01-20)
作者：Hui-Juan Qu:1.Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory for Research and Development of Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou, 571199, P. R. China. ; Kai-Wen Lin:1.Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory for Research and Development of Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou, 571199, P. R. China. ; Xiao-Liang Li:1.Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory for Research and Development of Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou, 571199, P. R. China. ; Hong-Ya Ou:1.Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory for Research and Development of Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou, 571199, P. R. China. ;
期刊：Chemical Constituents and Anti-Gastric Ulcer Activity of Essential Oils of Alpinia officinarum (Zingiberaceae), Cyperus rotundus (Cyperaceae), and Their Herbal Pair
影响因子 ：2.9
引用产品： 人胃黏膜上皮细胞
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LncRNA NUTM2A-AS1 positively modulates TET1 and HIF‐1A to enhance gastric cancer tumorigenesis and drug resistance by sponging miR‐376a (2010-00-20)
作者：Ji Wang:1.Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, China. ; Ziyang Yu:1.Department of gynaecology and obstetrics, The Second Affiliated Hospital of Soochow University, Suzhou, China. ; Jun Wang:1.Department of General Surgery, The Fifth People's Hospital of Wujiang, Suzhou, China. ; Yidan Shen:1.Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, China. ; Junlan Qiu:1.Department of Oncology and Hematology, The Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical University, Suzhou, China. ; Zhixiang Zhuang:1.Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, China. ;
期刊：LncRNA NUTM2A-AS1 positively modulates TET1 and HIF‐1A to enhance gastric cancer tumorigenesis and drug resistance by sponging miR‐376a
影响因子 ：4.0
引用产品： 人胃黏膜上皮细胞
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