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产品概述
名称 | NCI-N87 [N87] (人胃癌细胞) (STR鉴定正确) |
别称 | NCI-N87 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:3 |
推荐换液频率 | 2-3次/周 |
背景描述 | NCI-N87细胞表达表面糖蛋白癌胚抗原(CEA)和TAG72,并且没有左旋多巴胺脱羧酶(DDC)活性。NCI-N87细胞的血管活性的肠肽(VIP)受体活性极低并缺乏胃泌激素受体,它们表达蕈毒碱胆碱受体。没有证据表明,NCI-N87细胞存在N-myc、L-myc、myb和EGF受体基因的重组。NCI-N87细胞表达的c-myc和c-erb-B2 RNA水平与其它细胞株相当。以下基因NCI-N87细胞不表达:N-myc、L-myc、c-cis、IGF-2或胃泌激素释放肽。据报道,NCI-N87细胞的植板率为4.3%。 |
年龄(性别) | 男性 |
组织来源 | 胃癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 胃癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~48-80 hours |
致瘤性 | Yes, the cells form tumors in athymic nude mice. |
受体表达情况 | acetylcholine, muscarinic, expressed |
保藏机构 | ATCC; CRL-5822 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 8,12 D2S1338 23,24 D3S1358 14 D5S818 12,13 D7S820 10,11 D8S1179 14 D13S317 8,11 D16S539 9,13 D18S51 17 D19S433 14,14.2 D21S11 30 FGA 20,21 PentaD 12 PentaE 5 TH01 9 TPOX 9,11 vWA 15,16 D6S1043 12 D12S391 16,21 D2S441 11,12 -
STR鉴定图
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参考文献
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Quercetin induces ferroptosis in gastric cancer cells by targeting SLC1A5 and regulating the p-Camk2/p-DRP1 and NRF2/GPX4 Axes (2024-01-06)
期刊:FREE RADICAL BIOLOGY AND MEDICINE
DOI:10.1016/j.freeradbiomed.2024.01.002
影响因子 :7.4
引用产品: AGS 细胞 , HGC-27 细胞 , MKN-7 [MKN7; MKN 7] 细胞 , MKN-45 细胞 , SNU-1 细胞 , NCI-N87 [N87] 细胞 , Ham's F-12 培养基
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Homeobox Protein BarH-like 1 Promotes Gastric Cancer Progression by Activating Coiled-Coil Domain-Containing Protein 178 (2024-02-15)
期刊:DIGESTIVE DISEASES AND SCIENCES
DOI:10.1007/s10620-024-08312-0
影响因子 :3.1
引用产品: 人胃黏膜上皮细胞 , MKN-45 细胞 , AGS 细胞 , NCI-N87 [N87] 细胞 , KATO III [KATO 3] 细胞 , THP-1 细胞
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KBTBD2 promotes proliferation and migration of gastric cancer via activating EGFR signaling pathway (2024-01-04)
期刊:PATHOLOGY RESEARCH AND PRACTICE
影响因子 :2.8
引用产品: AGS 细胞 , NCI-N87 [N87] 细胞 , MKN-45 细胞 , SUN-1 细胞 , HGC-27 细胞 , GES-1 细胞
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Overcoming the On-Target Toxicity in Antibody-Mediated Therapies via an Indirect Active Targeting Strategy (2023-01-22)
期刊:Advanced Science
影响因子 :15.1
引用产品: 4T1 细胞 , MCF7 [MCF-7] 细胞 , A549 [A-549] 细胞 , A20 细胞 , RM-1 细胞 , NCI-N87 [N87] 细胞 , TE-1 细胞 , P388D1 细胞 , HPAC 细胞
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Tumor-Derived Exosomal LINC01480 Upregulates VCAM1 Expression by Acting as a Competitive Endogenous RNA of miR-204-5p to Promote Gastric Cancer Progression (2023-12-22)
期刊:ACS Biomaterials Science & Engineering
DOI:10.1021/acsbiomaterials.3c00394
影响因子 :5.8
引用产品: AGS 细胞 , SNU-1 细胞 , NCI-N87 [N87] 细胞 , GES-1 细胞
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Long noncoding RNA PSMA3-AS1 functions as a competing endogenous RNA to promote gastric cancer progression by regulating the miR-329-3p/ALDOA axis (2023-07-04)
期刊:Biology Direct
DOI:10.1186/s13062-023-00392-8
影响因子 :5.5
引用产品: GES-1 细胞 , HGC-27 细胞 , AGS 细胞 , NCI-N87 [N87] 细胞 , SNU-1 细胞 , 293T [HEK-293T] 细胞
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LINC01354 enhances the metastatic ability of gastric cancer cells by adjusting miR-153-5p/CADM2 expression (2023-06-15)
期刊:American Journal of Cancer Research
DOI:PMID:37424819
影响因子 :5.3
引用产品: HGC-27 细胞 , NCI-N87 [N87] 细胞 , MKN-7 [MKN7; MKN 7] 细胞 , MKN-45 细胞 , SNU-1 细胞
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Benzophenanthridine Alkaloid Chelerythrine Elicits Necroptosis of Gastric Cancer Cells via Selective Conjugation at the Redox Hyperreactive C-Terminal Sec498 Residue of Cytosolic Selenoprotein Thioredoxin Reductase (2023-09-28)
期刊:MOLECULES
影响因子 :4.6
引用产品: NCI-N87 [N87] 细胞 , AGS 细胞 , MKN-45 细胞 , RPMI-1640 培养基 , 特级胎牛血清
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Demethylase FTO enhances the PI3K/Akt signaling to promote gastric cancer malignancy (2023-03-27)
期刊:MEDICAL ONCOLOGY
DOI:10.1007/s12032-023-01990-2
影响因子 :3.4
引用产品: AGS 细胞 , SNU-1 细胞 , HGC-27 细胞 , NCI-N87 [N87] 细胞 , 293T [HEK-293T] 细胞
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Oncogenic transmembrane protein 158 drives the PI3K/Akt signaling pathway to accelerate gastric cancer cell growth (2023-11-13)
期刊:BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
DOI:10.1590/1414-431X2023e12943
影响因子 :2.3
引用产品: MKN-45 细胞 , SNU-1 细胞 , NCI-N87 [N87] 细胞 , AGS 细胞
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Celastrol inhibits gastric cancer cell proliferation, migration, and invasion via the FOXA1/CLDN4 axis (2023-04-20)
期刊:Toxicology Research
影响因子 :2.1
引用产品: GES-1 细胞 , AGS 细胞 , SGC-7901 细胞 , BGC-823 细胞 , NCI-N87 [N87] 细胞 , SNU-216 细胞 , MKN-28 细胞
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CircRPPH1 promotes the stemness of gastric cancer cells by targeting miR-375/SLC7A11 axis (2022-10-03)
期刊:ENVIRONMENTAL TOXICOLOGY
影响因子 :4.1
引用产品: MKN-45 细胞 , MKN-45细胞专用培养基 , AGS 细胞 , AGS细胞专用培养基 , Hs-746T 细胞 , Hs-746T细胞专用培养基 , NCI-N87 [N87] 细胞 , NCI-N87 [N87]细胞专用培养基 , HGC-27 细胞 , HGC-27细胞专用培养基 , GES-1 细胞 , GES-1细胞专用培养基
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Silencing of Nudix type 5 represses proliferation and invasion and enhances chemosensitivity of gastric carcinoma cells by affecting the AKT/GSK-3β/β-catenin pathway (2003-02-20)
期刊:Toxicology And Applied Pharmacology
DOI:10.1016/j.taap.2022.115968
影响因子 :3.8
引用产品: SGC-7901 细胞 , HGC-27 细胞 , MKN-45 细胞 , NCI-N87 [N87] 细胞 , GES-1 细胞
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ZNF521/EBF1 axis regulates AKR1B1 to promote the proliferation, migration, and invasion of gastric cancer cells (2022-11-17)
期刊:KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
影响因子 :3.0
引用产品: GES-1 细胞 , AGS 细胞 , NCI-N87 [N87] 细胞
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Knockdown of kinesin family member 2C restricts cell proliferation and induces cell cycle arrest in gastric cancer (2022-11-30)
期刊:HISTOLOGY AND HISTOPATHOLOGY
影响因子 :2.1
引用产品: 特级胎牛血清 , AGS 细胞 , MKN-45 细胞 , NCI-N87 [N87] 细胞 , SNU-1 细胞
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Isorhamnetin Promotes MKN-45 Gastric Cancer Cell Apoptosis by Inhibiting PI3K-Mediated Adaptive Autophagy in a Hypoxic Environment (2007-01-20)
期刊:Journal Of Agricultural And Food Chemistry
影响因子 :6.1
引用产品: MKN-28 细胞 , AGS 细胞 , NCI-N87 [N87] 细胞
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Circ_0021087 acts as a miR-184 sponge and represses gastric cancer progression by adsorbing miR-184 and elevating FOSB expression (2006-01-20)
期刊:European Journal Of Clinical Investigation
影响因子 :5.5
引用产品: AGS 细胞 , NCI-N87 [N87] 细胞 , GES-1 细胞
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Knockdown of Serum- and Glucocorticoid-Regulated Kinase 1 Enhances Cisplatin Sensitivity of Gastric Cancer Through Suppressing the Nuclear Factor Kappa-B Signaling Pathway. (2011-01-20)
期刊:Balkan Medical Journal
DOI:10.5152/balkanmedj.2021.21114
影响因子 :3.0
引用产品: NCI-N87 [N87] 细胞 , HGC-27 细胞
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Ubiquitin-specific protease 1 overexpression indicates poor prognosis and promotes proliferation, migration, and invasion of gastric cancer cells (2012-01-20)
期刊:Tissue & Cell
DOI:10.1016/j.tice.2021.101723
影响因子 :2.6
引用产品: NCI-N87 [N87] 细胞 , AGS 细胞 , MKN-45 细胞 , SNU-1 细胞 , GES-1 细胞
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Nucleoporin 37 promotes the cell proliferation, migration, and invasion of gastric cancer through activating the PI3K/AKT/mTOR signaling pathway (2012-01-20)
期刊:In Vitro Cellular & Developmental Biology - Animal
DOI:10.1007/s11626-021-00627-w
影响因子 :2.1
引用产品: NCI-N87 [N87] 细胞 , SNU-1 细胞
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Circ_0032821 Facilitates Gastric Cancer Cell Proliferation, Migration, Invasion and Glycolysis by Regulating MiR-1236-3p/HMGB1 Axis (2010-00-20)
期刊:Cancer Management and Research
影响因子 :3.3
引用产品: MKN-45 细胞 , NCI-N87 [N87] 细胞 , HGC-27 细胞 , AGS 细胞 , RPMI-1640 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100× , GES-1 细胞
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RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer (2010-09-20)
期刊:Oncogene
影响因子 :8.8
引用产品: NCI-N87 [N87] 细胞
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Pyrotinib treatment on HER2-positive gastric cancer cells promotes the released exosomes to enhance endothelial cell progression, which can be counteracted by apatinib (2003-09-20)
期刊:Onco Targets and Therapy
影响因子 :4.0
引用产品: NCI-N87 [N87] 细胞
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