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产品概述
产品简介
人骨髓间充质干细胞成脂诱导分化培养基专门为人骨髓间充质干细胞成脂诱导分化而开发，针对人骨髓间充质干细胞的特性优化分化试剂的配方，可增加人骨髓间充质干细胞的成脂分化效果。
本产品含血清成分，仅用于科研用途，不可用于诊断、治疗、临床及其他用途。
成脂诱导分化培养基A液：
成分名称 | 添加体积 |
成脂分化专用基础培养基A Basal Medium For Stem Cells Adipogenic Differentiation A | 177 mL |
成脂分化专用胎牛血清（FBS） Fetal Bovine Serum For Stem Cells Adipogenic Differentiation | 20 mL |
人骨髓间充质干细胞成脂分化添加物A① Supplement For hBMSCs Adipogenic Differentiation A① | 2.8 mL |
人骨髓间充质干细胞成脂分化添加物A② Supplement For hBMSCs Adipogenic Differentiation A② | 200 μL |
成脂诱导分化培养基B液：
成分名称 | 添加体积 |
成脂分化专用基础培养基B Basal Medium For Stem Cells Adipogenic Differentiation B | 177.2 mL |
成脂诱导分化专用胎牛血清（FBS） Fetal Bovine Serum For Stem Cells Adipogenic Differentiation | 20 mL |
人骨髓间充质干细胞成脂分化添加物B Supplement For hBMSCs Adipogenic Differentiation B | 2.8 mL |
辅助试剂：
成分名称 | 添加体积 |
油红O 染色液 Oil Red O Solution | 10 mL |
明胶包被液 Gelatin Solution | 10 mL |
注：各成分请根据试剂管上标签标示温度保存。
产品说明
| 产品规格 | 400mL |
| 储存条件 | 按标签所示温度保存，避光储存 |
| 运输条件 | 冰袋冷藏运输 |
| 有效期 | 一年 |
注意事项
1.因为培养基的成分较多，请在配制过程中严格注意无菌操作。
2. A、B交替诱导是为了减轻A液中试剂对干细胞的影响，如果您的干细胞状态较好可在前7天先只使用A液进行刺激诱导（中途每2~3天更换新鲜A液），待脂滴快速出现后，再进行两种培养基交替诱导的操作。
3.血清离心：血清中可能会存在白色絮状沉淀物，这通常是胆固醇、脂肪酸酯以及一些蛋白质析出导致，属于正常现象，该现象不影响细胞的培养和诱导分化。若您欲去除这些絮状沉淀物，可以将血清分装至无菌离心管内，以400-600 g离心5 min，取上清液使用。但是我们不建议您以过滤的方法去除这些絮状沉淀物，一方面它可能会阻塞您的过滤膜；另一方面，过滤血清这种行为可能会导致血清中部分营养成分的流失。
参考文献
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Effect of psoralen on the regulation of osteogenic differentiation induced by periodontal stem cell-derived exosomes (2023-06-03)
作者：Jie Yu:1.School of Stomatology, Qingdao University, Qingdao, 266023, China. ; Xiaonan Wu:1.Qingdao Stomatological Hospital Affiliated to Qingdao University, No.17 Dexian Road, Shinan District, Qingdao, 266001, Shandong, China. ; Wenyi Zhang:1.Qingdao Stomatological Hospital Affiliated to Qingdao University, No.17 Dexian Road, Shinan District, Qingdao, 266001, Shandong, China. ; Fuhang Chu:1.Qingdao Stomatological Hospital Affiliated to Qingdao University, No.17 Dexian Road, Shinan District, Qingdao, 266001, Shandong, China. ; Qi Zhang:1.School of Stomatology, Qingdao University, Qingdao, 266023, China. ; Meihua Gao:1.Qingdao Stomatological Hospital Affiliated to Qingdao University, No.17 Dexian Road, Shinan District, Qingdao, 266001, Shandong, China. ; Yingjie Xu:1.Qingdao Stomatological Hospital Affiliated to Qingdao University, No.17 Dexian Road, Shinan District, Qingdao, 266001, Shandong, China. xyjdywe@163.com. ; Yingtao Wu:1.School of Stomatology, Qingdao University, Qingda
期刊：Effect of psoralen on the regulation of osteogenic differentiation induced by periodontal stem cell-derived exosomes
DOI：10.1007/s13577-023-00918-2
影响因子 ：4.3
引用产品： 特级胎牛血清 , 人骨髓间充质干细胞成脂诱导分化培养基 , 磷酸盐缓冲液 (PBS) 1× , 人骨髓间充质干细胞成骨诱导分化培养基
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The bone mesenchymal stem cell-derived exosomal miR-146a-5p promotes diabetic wound healing in mice via macrophage M1/M2 polarization (2023-10-18)
作者：Xijie Zhou:1.Department of Hand and Microsurgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China. Electronic address: 493565783@qq.com. ; Chenhao Ye:1.Department of Hand and Microsurgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China. Electronic address: ych20000628@163.com. ; Liangfu Jiang:1.Department of Hand and Microsurgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China. Electronic address: jiangliangfu@wmu.edu.cn. ; Xuwei Zhu:1.Department of Hand and Microsurgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China. Electronic address: Drzhuxuwei@163.com. ; Feiya Zhou:1.Department of Hand and Microsurgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China. Electronic address: feiyazhou@yeah.net. ; Meizi Xia:1.Department of Nephrology, Wenzhou Geriatric Hospital, Wenzhou, 325000, China. Electroni
期刊：The bone mesenchymal stem cell-derived exosomal miR-146a-5p promotes diabetic wound healing in mice via macrophage M1/M2 polarization
影响因子 ：4.1
引用产品： THP-1 细胞 , 人骨髓间充质干细胞成脂诱导分化培养基 , 人骨髓间充质干细胞成骨诱导分化培养基
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Copper-deposited diatom-biosilica enhanced osteogenic potential in periodontal ligament stem cells and rat cranium (2023-02-11)
作者：Xu Qiu:1.Department of Periodontology, The Affiliated Hospital of Qingdao University, Qingdao, China. ; 2.College of Stomatology, Qingdao University, Qingdao, China. ; Chao Feng:1.College of Marine Life Science, Ocean University of China, Qingdao, China. ; Wenxuan Wang:1.Department of Periodontology, The Affiliated Hospital of Qingdao University, Qingdao, China. ; 2.College of Stomatology, Qingdao University, Qingdao, China. ; Guangsheng Wu:1.Department of Stomatology, Qingdao Special Servicemen Recuperation Center of PLA Navy, Qingdao, China. ; Yingzhe Hu:1.Department of Periodontology, The Affiliated Hospital of Qingdao University, Qingdao, China. ; 2.College of Stomatology, Qingdao University, Qingdao, China. ; Shuhan Li:1.Department of Periodontology, The Affiliated Hospital of Qingdao University, Qingdao, China. ; 2.College of Stomatology, Qingdao University, Qingdao, China. ; Xiangru Gao:1.Department of Periodontology, The Affiliated Hospital of Qingdao University, Qingdao,
期刊：Copper-deposited diatom-biosilica enhanced osteogenic potential in periodontal ligament stem cells and rat cranium
影响因子 ：3.4
引用产品： 人骨髓间充质干细胞成脂诱导分化培养基
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