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产品概述
名称 | H4 (人脑神经胶质瘤细胞) (STR鉴定正确) |
别称 | H-4 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | H4细胞建系于1973年;它衍生于一个患神经胶质瘤的37岁病人的脑组织。H4细胞的致瘤特性己经被屏蔽,细胞接种动物一般不产生肿瘤结节。H4细胞具有修复MNNG损伤5型腺病毒的能力。 |
年龄(性别) | 男性;37岁 |
组织来源 | 脑组织 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 胶质瘤细胞 |
生物安全等级 | BSL-1 |
致瘤性 | No, in immunosuppressed mice.Yes, in semisolid medium. |
保藏机构 | ATCC; HTB-148 BCRJ; 0263 |
STR鉴定
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STR位点信息
Amelogenin X, Y CSF1PO 10,12 D2S1338 18,25 D3S1358 17,18 D5S818 10,12 D7S820 8,11 D8S1179 9,12 D13S317 12 D16S539 11,12 D18S51 14,16 D19S433 12,14 D21S11 30,31 FGA 19,25 PentaD 10,12 PentaE 5,12 TH01 7,9 TPOX 8,11 vWA 14,18 D6S1043 19 D12S391 15,20 D2S441 10,11 -
STR鉴定图
参考文献
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CircIQGAP1 regulates RCAN1 and RCAN2 through the mechanism of ceRNA and promotes the growth of malignant glioma (2023-10-31)
作者:Xiang Huang:1.Department of Neurosurgery, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong 226001, China. ; Ziheng Wang:1.Clinical Biobank, Affiliated Hospital of Nantong University, Nantong 226001, China. ; Mengruo Song:1.The Sixth Affiliated Hospital of Nantong University, Yancheng Third People's Hospital, Yancheng224000, China. ; He Huan:1.Department of Neurosurgery, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong 226001, China. ; Zishu Cai:1.Department of Neurosurgery, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong 226001, China. ; Bing Wu:1.Department of Neurosurgery, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong 226001, China. ; Jianhong Shen:1.Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong 226001, China. ; You Lang Zhou:1.Research Center of Clinical Medicine, Affiliated Hospita
期刊:CircIQGAP1 regulates RCAN1 and RCAN2 through the mechanism of ceRNA and promotes the growth of malignant glioma
DOI:10.1016/j.phrs.2023.106979
影响因子 :9.3
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