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产品概述
| 名称 | NCI-H23 (人非小细胞肺癌细胞) (STR鉴定正确) |
| 别称 | NCI.H23; NCI H23; H23; H-23; NCIH23 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
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| 推荐传代比例 | 1:3-1:6 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | NCI-H23细胞源于一位51岁患有非小细胞肺癌黑人男性患者的治疗前的肿瘤组织，表达C-myc、L-myc、v-src、v-abl、v-erbB、c-raf 1、Ha-ras、Ki-ras、N-ras RNAs；NCI-H23细胞携带K-ras 12突变；p53基因246位密码子突变ATC→ATG；表达PDGF A和B链的异源mRNA；表达TGFα、TGFβ和EGFR；角蛋白5、8和18阳性，波形蛋白阳性，神经丝蛋白阴性，左旋多巴脱氢酶阴性；据报道，NCI-H23细胞在软琼脂中形成克隆的效率为9.7%。 |
| 年龄（性别） | 男性；51岁 |
| 组织来源 | 肺 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肺癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~38 hours |
| 基因表达情况 | myc+; src+; abl+; erb+; ras+; sis - |
| 保藏机构 | ATCC; CRL-5800 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10 D2S1338 18,23 D3S1358 15 D5S818 12,13 D7S820 9,10 D8S1179 15 D13S317 12 D16S539 11 D18S51 14 D19S433 12,14 D21S11 30 FGA 24 PentaD 8 PentaE 7,17 TH01 6 TPOX 8,9 vWA 16,17 D6S1043 12 D12S391 15,17 D2S441 11 -
STR鉴定图
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参考文献
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Lnc AC016727.1/BACH1/HIF-1 α signal loop promotes the progression of non-small cell lung cancer (2023-11-10)
作者：Li Zhang:1.Thoracic Surgery Laboratory, Xuzhou Medical University, Xuzhou, 221006, Jiangsu Province, China. ; 2.Department of Thoracic Surgery, Affiliated Hospital of Xuzhou Medical University, 99 West Huaihai Road, Xuzhou, 221006, Jiangsu Province, China. ; Jingtian Liang:1.Thoracic Surgery Laboratory, Xuzhou Medical University, Xuzhou, 221006, Jiangsu Province, China. ; 2.Department of Thoracic Surgery, Affiliated Hospital of Xuzhou Medical University, 99 West Huaihai Road, Xuzhou, 221006, Jiangsu Province, China. ; Hao Qin:1.Thoracic Surgery Laboratory, Xuzhou Medical University, Xuzhou, 221006, Jiangsu Province, China. ; 2.Department of Thoracic Surgery, Affiliated Hospital of Xuzhou Medical University, 99 West Huaihai Road, Xuzhou, 221006, Jiangsu Province, China. ; Yin Lv:1.Thoracic Surgery Laboratory, Xuzhou Medical University, Xuzhou, 221006, Jiangsu Province, China. ; 2.Department of Thoracic Surgery, Affiliated Hospital of Xuzhou Medical University, 99 West Huaihai Road, X
期刊：Lnc AC016727.1/BACH1/HIF-1 α signal loop promotes the progression of non-small cell lung cancer
DOI：10.1186/s13046-023-02875-y
影响因子 ：11.3
引用产品： A549 [A-549] 细胞 , NCI-H1299 细胞 , NCI-H292 细胞 , NCI-H23 细胞 , NCI-H1703 细胞 , NCI-H226 [H226] 细胞 , BEAS-2B 细胞
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GRIN2D knockdown suppresses the progression of lung adenocarcinoma by regulating the E2F signalling pathway (2023-04-19)
作者：Zhitao Chen:1.Department of Thoracic Surgery, Jinan Central Hospital Affiliated to Shandong First Medical University, Jinan 250013, Shandong, China. ; Yanhui Song:1.Department of Internal Medicine, Rongfu Military Hospital of Jining City, Jining 272101, Shandong, China. ; Peipei Li:1.Department of General Surgery, Jinan Central Hospital Affiliated to Shandong First Medical University, Jinan 250013, Shandong, China. ; Wei Gao:1.Department of Pathology, Jinan Central Hospital Affiliated to Shandong First Medical University, Jinan 250013, Shandong, China. Electronic address: gw98755@163.com. ;
期刊：GRIN2D knockdown suppresses the progression of lung adenocarcinoma by regulating the E2F signalling pathway
DOI：10.1016/j.cellsig.2023.110685
影响因子 ：4.8
引用产品： NCI-H1299 细胞 , A549 [A-549] 细胞 , NCI-H23 细胞 , NCI-H1975 细胞
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FATP2 regulates non-small cell lung cancer by mediating lipid metabolism through ACSL1 (2023-05-06)
作者：Yinghua Chen:1.Department of Oncology and Hematology, Chongqing University Central Hospital, Chongqing 400014, China. ; Yu Zhao:1.Department of Oncology and Hematology, The Ninth People's Hospital of Chongqing 400700, China. ; Yuan Deng:1.Department of Oncology and Hematology, Chongqing University Central Hospital, Chongqing 400014, China. ; Yan Yang:1.Department of Oncology and Hematology, Chongqing University Central Hospital, Chongqing 400014, China. ; Lijun Xu:1.Department of Oncology and Hematology, Chongqing University Central Hospital, Chongqing 400014, China. ; Jianwei Fu:1.Department of Oncology and Hematology, Chongqing University Central Hospital, Chongqing 400014, China. Electronic address: fjw450056802@163.com. ;
期刊：FATP2 regulates non-small cell lung cancer by mediating lipid metabolism through ACSL1
DOI：10.1016/j.tice.2023.102105
影响因子 ：2.6
引用产品： A549 [A-549] 细胞 , HCC827 细胞 , NCI-H1299 细胞 , NCI-H23 细胞 , BEAS-2B 细胞 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗)，100×
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Circular circRANGAP1 Contributes to Non-small Cell Lung Cancer Progression by Increasing COL11A1 Expression Through Sponging miR-653-5p (2023-05-16)
作者：Min Chen:1.Department of Respiratory and Critical Care Medicine, Shanghai Tongren Hospital, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, No 1111 XianXia Road, Changning District, Shanghai, 200335, China. CM1149@shtrhospital.come. ; Jing Zhang:1.Department of Respiratory and Critical Care Medicine, Shanghai Tongren Hospital, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, No 1111 XianXia Road, Changning District, Shanghai, 200335, China. ; Jian Zeng:1.Department of Respiratory and Critical Care Medicine, Shanghai Tongren Hospital, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, No 1111 XianXia Road, Changning District, Shanghai, 200335, China. ; Yiming Yu:1.Department of Respiratory and Critical Care Medicine, Shanghai Tongren Hospital, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, No 1111 XianXia Road, Changning District, Shanghai, 200335, China. ; Chenlin Gu:1.Department of Respiratory and Critica
期刊：Circular circRANGAP1 Contributes to Non-small Cell Lung Cancer Progression by Increasing COL11A1 Expression Through Sponging miR-653-5p
DOI：10.1007/s10528-023-10393-x
影响因子 ：2.4
引用产品： NCI-H23 细胞 , A549 [A-549] 细胞 , NCI-H1975 细胞 , BEAS-2B 细胞 , NCI-H23细胞专用培养基 , A549 [A-549]细胞专用培养基 , NCI-H1975细胞专用培养基 , BEAS-2B细胞专用培养基
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IMP4 Silencing Inhibits the Malignancy of Lung Adenocarcinoma via ERK Pathway (2022-10-22)
作者：Ruzhen Li:1.Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China. ; Zhaohui Han:1.Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China. ; Wei Ma:1.Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China. ; Lin Zhang:1.Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China. ; Xiangwei Zhang:1.Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China. ; Yuanzhu Jiang:1.Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China. ; Wei Dong:1.Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China. ;
期刊：IMP4 Silencing Inhibits the Malignancy of Lung Adenocarcinoma via ERK Pathway
影响因子 ：4.5
引用产品： NCI-H1975 细胞 , NCI-H1299 细胞 , A549 [A-549] 细胞 , NCI-H23 细胞 , 16HBE 细胞
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