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产品概述
名称 | A549/DDP (人肺腺癌耐顺铂株)(STR鉴定正确) |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:3 |
推荐换液频率 | 2-3次/周 |
注意事项 | 耐药株生长速度慢,1:2传代一周左右可以再次传代,细胞形态比较梭长。当细胞生长速度过慢时,可以采用以下方式进行扩增培养:用含低浓度药物的培养基连续培养2周,期间根据细胞密度,需要传代时,传代处理。待细胞状态恢复后,提高培养基中药物的浓度进行培养,此时梯度加药,每个浓度培养一个代次(即持续培养到长满传代,即换下一个浓度),之后细胞状态没有问题的话,持续用加药培养基进行培养即可。 |
背景描述 | A549/DDP细胞是在A549细胞基础上,通过剂量递增法建立的耐DDP药物的细胞株。 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 10,12 D2S1338 24 D3S1358 16 D5S818 11 D7S820 8,11 D8S1179 13,14 D13S317 11 D16S539 11,12 D18S51 14,17 D19S433 13 D21S11 29 FGA 23 PentaD 9 PentaE 7,11 TH01 8,9.3 TPOX 8,11 vWA 14 D6S1043 11,13 D12S391 18 D2S441 10,13 -
STR鉴定图
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参考文献
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Self-Assembly of Erlotinib-Platinum(II) Complexes for Epidermal Growth Factor Receptor-Targeted Photodynamic Therapy (2024-01-06)
期刊:JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1021/acs.jmedchem.3c01889
影响因子 :7.3
引用产品: A549 [A-549] 细胞 , A549/DDP 细胞 , A549/Tax 细胞 , A-431 细胞 , MRC-5 细胞
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Assessment of Mononuclear/Dinuclear copper acylhydrazone complexes for lung cancer treatment (2024-01-14)
期刊:BIOORGANIC CHEMISTRY
DOI:10.1016/j.bioorg.2024.107122
影响因子 :5.1
引用产品: A549 [A-549] 细胞 , A549/DDP 细胞 , WI-38 细胞
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Role and mechanism of COL3A1 in regulating the growth, metastasis, and drug sensitivity in cisplatin-resistant non-small cell lung cancer cells (2024-03-26)
期刊:CANCER BIOLOGY & THERAPY
DOI:10.1080/15384047.2024.2328382
影响因子 :3.6
引用产品: A549 [A-549] 细胞 , NCI-H460 [H460] 细胞 , Ham's F-12K完全培养基 (含10%FBS) , RPMI-1640完全培养基 (含10%FBS) , HBE cell , A549/DDP 细胞
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Anlotinib Inhibits Cisplatin Resistance in Non-Small-Cell Lung Cancer Cells by Inhibiting MCL-1 Expression via MET/STAT3/Akt Pathway (2024-03-04)
期刊:Canadian Respiratory Journal
影响因子 :2.2
引用产品: A549 [A-549] 细胞 , A549/DDP 细胞 , Ham's F-12K 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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Hypoxia-triggered tumor specific glutamine inhibition for reversing cisplatin resistance of chemotherapy (2023-11-27)
期刊:CHEMICAL ENGINEERING JOURNAL
影响因子 :15.1
引用产品: A549 [A-549] 细胞 , A549/DDP 细胞 , 青霉素-链霉素溶液 (双抗),100× , HK-2 细胞
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A Clinically Translatable Ternary Platinum(IV) Prodrug for Synergistically Reversing Drug Resistance (2023-03-10)
期刊:JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1021/acs.jmedchem.2c01924
影响因子 :7.3
引用产品: A549/DDP 细胞
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A cisplatin and disulfiram co-loaded inclusion complex overcomes drug resistance by inhibiting cancer cell stemness in non-small cell lung cancer (2023-12-22)
期刊:JOURNAL OF DRUG TARGETING
DOI:10.1080/1061186X.2023.2298844
影响因子 :4.5
引用产品: A549/DDP 细胞
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Mixed-ligand copper(II) hydrazone complexes: Synthesis, structure, and anti-lung cancer properties (2023-01-19)
期刊:JOURNAL OF MOLECULAR STRUCTURE
DOI:10.1016/j.molstruc.2023.134986
影响因子 :3.8
引用产品: A549 [A-549] 细胞 , A549/DDP 细胞 , HK-2 细胞
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Mechanism of PWAR6 regulating cisplatin drug sensitivity in non-small cell lung cancer through miR-577/PHACTR1 (2023-11-03)
期刊:GENE
DOI:10.1016/j.gene.2023.147954
影响因子 :3.5
引用产品: A549 [A-549] 细胞 , A549/DDP 细胞
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Andrographis modulates cisplatin resistance in lung cancer via miR-155-5p/SIRT1 axis (2023-08-02)
期刊:FUNCTIONAL & INTEGRATIVE GENOMICS
DOI:10.1007/s10142-023-01186-x
影响因子 :2.9
引用产品: A549 [A-549] 细胞 , A549/DDP 细胞 , NCI-H460 [H460] 细胞 , NCI-H1299 细胞 , BEAS-2B 细胞
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Synthesis and antitumor activities of 4-(1H-indol-1-yl)-4-oxobutanoic acid spliced podophyllotoxin derivatives (2023-01-06)
期刊:SYNTHETIC COMMUNICATIONS
DOI:10.1080/00397911.2023.2164863
影响因子 :2.1
引用产品: A549 [A-549] 细胞 , A549/DDP 细胞 , MCF7 [MCF-7] 细胞 , HL-7702 [L-02; LO2] 细胞
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Inhibition of autophagy potentiates the cytotoxicity of the irreversible FGFR1-4 inhibitor FIIN-2 on lung adenocarcinoma (2022-08-30)
期刊:Cell Death & Disease
DOI:10.1038/s41419-022-05201-0
影响因子 :9.7
引用产品: A549 [A-549] 细胞 , A549/DDP 细胞
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CXCR5 guides migration and tumor eradication of anti-EGFR chimeric antigen receptor T cells (2007-01-20)
期刊:Molecular Therapy-Oncolytics
DOI:10.1016/j.omto.2021.07.003
影响因子 :5.7
引用产品: A549/DDP 细胞 , COS-1 细胞 , K-562 [K562] 细胞 , NCI-H929 细胞
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Silencing ZIC2 abrogates tumorigenesis and anoikis resistance of non-small cell lung cancer cells by inhibiting Src/FAK signaling (2005-01-20)
期刊:Molecular Therapy-Oncolytics
DOI:10.1016/j.omto.2021.05.008
影响因子 :5.7
引用产品: NCI-H1975 细胞 , NCI-H1395 细胞 , NCI-H520 细胞 , A549/DDP 细胞 , NCI-H1299 细胞
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Circ_0017956 promotes the proliferation and metastasis of non-small cell lung cancer through regulating miR-515-5p/ITGB8 axis (2005-01-20)
期刊:Cell Cycle
DOI:10.1080/15384101.2021.1919829
影响因子 :4.3
引用产品: A549/DDP 细胞 , NCI-H1299 细胞 , HBE135-E6E7 细胞
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Circ_0006988 promotes the proliferation, metastasis and angiogenesis of non-small cell lung cancer cells by modulating miR-491-5p/MAP3K3 axis (2006-01-20)
期刊:Cell Cycle
DOI:10.1080/15384101.2021.1941612
影响因子 :4.3
引用产品: BEAS-2B , A549/DDP 细胞 , NCI-H1299 细胞 , RPMI-1640 培养基 , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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miR-324-3p reverses cisplatin resistance by inducing GPX4-mediated ferroptosis in lung adenocarcinoma cell line A549 (2003-01-20)
期刊:Biochemical and Biophysical Research Communications
DOI:10.1016/j.bbrc.2021.02.077
影响因子 :3.1
引用产品: A549 [A-549] 细胞 , A549/DDP 细胞
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Identification of circ_0058357 as a regulator in non-small cell lung cancer cells resistant to cisplatin by miR-361-3p/ABCC1 axis (2009-01-20)
期刊:Thoracic Cancer
影响因子 :2.9
引用产品: 人支气管上皮细胞 , A549 [A-549] 细胞 , A549/DDP 细胞 , NCI-H1299 细胞
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MicroRNA-106a-5p alleviated the resistance of cisplatin in lung cancer cells by targeting Jumonji domain containing 6 (2010-01-20)
期刊:Transplant Immunology
DOI:10.1016/j.trim.2021.101478
影响因子 :2.0
引用产品: A549 [A-549] 细胞 , A549/DDP 细胞
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Optimized Fluorescent Probe for Specific Imaging of Glutathione S-Transferases in Living Cells and Mice (2003-00-20)
期刊:Chemistry-An Asian Journal
影响因子 :4.1
引用产品: A549/DDP 细胞
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Overexpression of close homolog of L1 enhances the chemosensitivity of lung cancer cells via inhibition of the Akt pathway (2008-00-20)
期刊:Oncology Letters
影响因子 :2.9
引用产品: A549 [A-549] 细胞 , A549/DDP 细胞
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Demethoxycurcumin increases the sensitivity of cisplatin‑resistant non‑small lung cancer cells to cisplatin and induces apoptosis by activating the caspase signaling pathway (2009-00-20)
期刊:Oncology Letters
影响因子 :2.9
引用产品: A549/DDP 细胞 , A549/DDP细胞专用培养基
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A ratiometric near-infrared fluorescence strategy based on spiropyran in situ switching for tracking dynamic changes of live-cell lysosomal pH (2003-09-20)
期刊:Dyes And Pigments
DOI:10.1016/j.dyepig.2019.03.060
影响因子 :4.5
引用产品: Hep G2 细胞 , MCF7 [MCF-7] 细胞 , PC-3 细胞 , A549/DDP 细胞
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Targeting Polo-like Kinase 1 by a Novel Pyrrole-Imidazole Polyamide–Hoechst Conjugate Suppresses Tumor Growth In Vivo (2005-08-20)
期刊:Molecular Cancer Therapeutics
DOI:10.1158/1535-7163.MCT-17-0747
影响因子 :5.7
引用产品: Hela 细胞 , A549/DDP 细胞 , PUMC-HUVEC-T1
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A novel colorimetric and ratiometric fluorescent probe for selective detection of bisulfite in real samples and living cells (2012-08-20)
期刊:Dyes And Pigments
DOI:10.1016/j.dyepig.2018.12.009
影响因子 :4.5
引用产品: A549/DDP 细胞
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