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产品概述
| 名称 | J82 (人膀胱移行细胞癌) (STR鉴定正确) |
| 别称 | J-82; J 82; J82COT; J82 COT |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
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| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | J82细胞源自一名58岁患有膀胱移行细胞癌的白人男性。J82细胞内无桥粒，有大量粗面内质网及突出绒毛，可表达ras基因。 |
| 年龄（性别） | 男性；58岁 |
| 组织来源 | 器官：膀胱；疾病：移行细胞癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 膀胱癌细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | Yes, in nude mice. |
| 抗原表达情况 | HLA A2, Aw32, B5, B12, Cw5; Blood Type A |
| 保藏机构 | ATCC; HTB-1 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 10,11 D2S1338 19 D3S1358 16,18 D5S818 12,13 D7S820 9,11 D8S1179 8,13 D13S317 10,12 D16S539 11,12 D18S51 10,12 D19S433 12,13 D21S11 30 FGA 20,24 PentaD 9,13 PentaE 12,15 TH01 9.3 TPOX 11,12 vWA 17,18 D6S1043 19 D12S391 24 D2S441 10,13 -
STR鉴定图
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参考文献
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Transcriptomic insights into UTUC: role of inflammatory fibrosis and potential for personalized treatment (2024-01-05)
作者：Keqiang Li:1.Department of Urology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China. ; 2.Academy of Medical Sciences, Zhengzhou University, Zhengzhou, Henan Province, China. ; Zhenlin Huang:1.Department of Urology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China. ; Guoqing Xie:1.Department of Urology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China. ; 2.Academy of Medical Sciences, Zhengzhou University, Zhengzhou, Henan Province, China. ; Budeng Huang:1.Department of Urology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China. ; 2.Academy of Medical Sciences, Zhengzhou University, Zhengzhou, Henan Province, China. ; Liang Song:1.Department of Urology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China. ; 2.Academy of Medical Sciences, Zhengzhou University, Zhengzhou, Henan Province, China. ;
期刊：Transcriptomic insights into UTUC: role of inflammatory fibrosis and potential for personalized treatment
DOI：10.1186/s12967-023-04815-y
影响因子 ：7.4
引用产品： J82 细胞 , UM-UC-3 细胞
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CENPW knockdown inhibits progression of bladder cancer through inducing cell cycle arrest and apoptosis (2024-01-01)
作者：Peng Zhang:1.Guizhou Medical University, Guiyang, China. ; 2.Department of Urology, Guizhou Provincial People's Hospital, Guiyang, China. ; Qian Yang:1.Department of Gastroenterology, Guizhou Provincial People's Hospital, Guiyang, China. ; Xulong Chen:1.Guizhou Medical University, Guiyang, China. ; Xiaolong Chen:1.Department of Urology, Guizhou Provincial People's Hospital, Guiyang, China. ; Qing Wang:1.Department of Urology, Guizhou Provincial People's Hospital, Guiyang, China. ; Kun Chen:1.Department of Medical Genetics, Guizhou Provincial People's Hospital, Guiyang, China. ; Yu An:1.NHC Key Laboratory of Pulmonary Immune-Related Diseases, Guizhou Provincial People's Hospital, Guiyang, China. ; Kehua Jiang:1.Department of Urology, Guizhou Provincial People's Hospital, Guiyang, China. ; Fa Sun:1.Guizhou Medical University, Guiyang, China. ; 2.Department of Urology, Guizhou Provincial People's Hospital, Guiyang, China. ;
期刊：CENPW knockdown inhibits progression of bladder cancer through inducing cell cycle arrest and apoptosis
影响因子 ：3.9
引用产品： 5637 细胞 , T24 [T-24] 细胞 , RT4 细胞 , J82 细胞 , UM-UC-3 细胞 , SV-HUC-1 细胞
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WD repeat protein 54-mediator of ErbB2-driven cell motility 1 axis promotes bladder cancer tumorigenesis and metastasis and impairs chemosensitivity (2023-01-07)
作者：Xiaosong Wei:1.Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, People's Republic of China. ; Beibei Wang:1.Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, People's Republic of China. ; Zixin Wu:1.Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, People's Republic of China. ; Xiaoming Yang:1.Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, People's Republic of China. ; Yufeng Guo:1.Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, People's Republic of China. ; Yang Yang:1.Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, People's Republic of China. ; Zhiwei Fang:1.Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou
期刊：WD repeat protein 54-mediator of ErbB2-driven cell motility 1 axis promotes bladder cancer tumorigenesis and metastasis and impairs chemosensitivity
DOI：10.1016/j.canlet.2023.216058
影响因子 ：9.7
引用产品： SV-HUC-1 细胞 , UM-UC-3 细胞 , J82 细胞 , 5637 细胞 , SW 780 [SW-780, SW780] 细胞 , T24 [T-24] 细胞 , TCCSUP 细胞 , Ham's F-12K 培养基 , MEM (含NEAA) 培养基 , Leibovitz's L-15 培养基 , McCoy's 5A 培养基
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POLR3G promotes EMT via PI3K/AKT signaling pathway in bladder cancer (2023-11-07)
作者：Hualin Chen:1.Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. ; Lin Ma:1.Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. ; Wenjie Yang:1.Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. ; Yingjie Li:1.Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. ; Zhigang Ji:1.Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. ;
期刊：POLR3G promotes EMT via PI3K/AKT signaling pathway in bladder cancer
影响因子 ：4.8
引用产品： T24 [T-24] 细胞 , 5637 细胞 , RT4 细胞 , J82 细胞 , SV-HUC-1 细胞
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Label-free LC-MS/MS proteomics analyses reveal CLIC1 as a predictive biomarker for bladder cancer staging and prognosis (2023-01-16)
作者：Weifeng Wang:1.Department of Urology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. ; Guankai Huang:1.Department of Urology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. ; Hansen Lin:1.Department of Urology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. ; 2.Institute of Precision Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. ; Lei Ren:1.Department of Urology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. ; Liangmin Fu:1.Department of Urology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. ; 2.Institute of Precision Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. ; Xiaopeng Mao:1.Department of Urology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. ;
期刊：Label-free LC-MS/MS proteomics analyses reveal CLIC1 as a predictive biomarker for bladder cancer staging and prognosis
影响因子 ：4.7
引用产品： SV-HUC-1 细胞 , T24 [T-24] 细胞 , 5637 细胞 , J82 细胞 , RT-112 细胞 , EJ-1 细胞 , TCCSUP 细胞 , UM-UC-3 细胞
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Downregulation of CBX7 induced by EZH2 upregulates FGFR3 expression to reduce sensitivity to cisplatin in bladder cancer (2022-11-17)
作者：Jiannan Ren:1.Department of Urology, The Second Xiangya Hospital, Central South University, 410011, Changsha, Hunan, China. ; 2.Uro-Oncology Institute of Central South University, 410011, Changsha, Hunan, China. ; Haixin Yu:1.Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China. ; Wei Li:1.Department of Urology, The Second Xiangya Hospital, Central South University, 410011, Changsha, Hunan, China. ; 2.Uro-Oncology Institute of Central South University, 410011, Changsha, Hunan, China. ; Xin Jin:1.Department of Urology, The Second Xiangya Hospital, Central South University, 410011, Changsha, Hunan, China. jinxinxy2@csu.edu.cn. ; 2.Uro-Oncology Institute of Central South University, 410011, Changsha, Hunan, China. jinxinxy2@csu.edu.cn. ; Bin Yan:1.Department of Urology, The Second Xiangya Hospital, Central South University, 410011, Changsha, Hunan, China. bin.yan01@csu.edu.cn. ; 2.Uro-Oncology Institute of Central S
期刊：Downregulation of CBX7 induced by EZH2 upregulates FGFR3 expression to reduce sensitivity to cisplatin in bladder cancer
DOI：10.1038/s41416-022-02058-0
影响因子 ：9.1
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The oncogenic role of TFAP2A in bladder urothelial carcinoma via a novel long noncoding RNA TPRG1-AS1/DNMT3A/CRTAC1 axis (2022-11-21)
作者：Jiani He:1.Department of Surgical Oncology and Breast Surgery, The First Hospital of China Medical University, Shenyang 110001, Liaoning, PR China. ; Changming Dong:1.Department of Urology, The First Hospital of China Medical University, Shenyang 110001, Liaoning, PR China; Institute of Urology, China Medical University, Shenyang 110001, Liaoning, PR China. ; Hao Zhang:1.Department of Urology, The First Hospital of China Medical University, Shenyang 110001, Liaoning, PR China; Institute of Urology, China Medical University, Shenyang 110001, Liaoning, PR China. ; Yuanjun Jiang:1.Department of Urology, The First Hospital of China Medical University, Shenyang 110001, Liaoning, PR China; Institute of Urology, China Medical University, Shenyang 110001, Liaoning, PR China. ; Tao Liu:1.Department of Urology, The First Hospital of China Medical University, Shenyang 110001, Liaoning, PR China; Institute of Urology, China Medical University, Shenyang 110001, Liaoning, PR China. ; Xiaojun Ma
期刊：The oncogenic role of TFAP2A in bladder urothelial carcinoma via a novel long noncoding RNA TPRG1-AS1/DNMT3A/CRTAC1 axis
DOI：10.1016/j.cellsig.2022.110527
影响因子 ：4.9
引用产品： RT4 细胞 , T24 [T-24] 细胞 , J82 细胞 , 5637 细胞 , SV-HUC-1 细胞 , 人脐静脉内皮细胞 , 293T [HEK-293T] 细胞
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EFEMP2 suppresses epithelial-mesenchymal transition via Wnt/β-catenin signaling pathway in human bladder cancer (2008-09-20)
作者：Qiang Zhou:1.Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Song Chen:1.Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Mengxin Lu:1.Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Yongwen Luo:1.Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Gang Wang:1.Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, China. ; 2.Human Genetics Resource Preservation Center of Hubei Province, Wuhan, China. ; 3.Laboratory of Precision Medicine, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Yu Xiao:1.Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China. ; 2.Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, China. ; 3.Human Genetics Resource Preservation Center of Hubei Province, Wuhan, China. ; 4.Laboratory of Precision Medicine, Zhongnan Hospital of Wuhan University, Wuhan, China.
期刊：EFEMP2 suppresses epithelial-mesenchymal transition via Wnt/β-catenin signaling pathway in human bladder cancer
影响因子 ：9.2
引用产品： J82 细胞 , SW 780 [SW-780, SW780] 细胞
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miR-4324-RACGAP1-STAT3-ESR1 feedback loop inhibits proliferation and metastasis of bladder cancer (2001-09-20)
作者：Qiangqiang Ge:1.Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Mengxin Lu:1.Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Lingao Ju:1.Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, China. ; 2.Human Genetics Resource Preservation Center of Hubei Province, Wuhan, China. ; Kaiyu Qian:1.Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, China. ; 2.Human Genetics Resource Preservation Center of Hubei Province, Wuhan, China. ; Gang Wang:1.Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, China. ; 2.Human Genetics Resource Preservation Center of Hubei Province, Wuhan, China. ; 3.Laboratory of Precision Medicine, Zhongnan Hospital of Wuhan University, Wuhan, China. ; Chin-Lee Wu:1.Department of Urology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. ; Xuefeng Liu:1.Department of Pathology, Lomba
期刊：miR-4324-RACGAP1-STAT3-ESR1 feedback loop inhibits proliferation and metastasis of bladder cancer
影响因子 ：6.4
引用产品： J82 细胞
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MiR-34a-5p/PD-L1 axis regulates cisplatin chemoresistance of ovarian cancer cells (2012-09-20)
作者：Y Zuo:1.Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, China. ; 2.Department of Gynecology, Affiliated Yantai Yuhuangding Hospital, Medical College of Qingdao University, Yantai, China. ; W Zheng:1.Department of Gynecology, Affiliated Yantai Yuhuangding Hospital, Medical College of Qingdao University, Yantai, China. ; J Liu:1.Department of Gynecology, Affiliated Yantai Yuhuangding Hospital, Medical College of Qingdao University, Yantai, China. ; Q Tang:1.Department of Gynecology, Affiliated Yantai Yuhuangding Hospital, Medical College of Qingdao University, Yantai, China. ; S S Wang:1.Department of Gynecology, Affiliated Yantai Yuhuangding Hospital, Medical College of Qingdao University, Yantai, China. ; X S Yang:1.Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, China. ;
期刊：MiR-34a-5p/PD-L1 axis regulates cisplatin chemoresistance of ovarian cancer cells
DOI：10.4149/neo_2019_190202N106
影响因子 ：3.4
引用产品： A2780 细胞 , J82 细胞 , 293T [HEK-293T] 细胞
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