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产品概述
产品简介
RPMI-1640是Moore等人于1967年在美国纽约州法罗市的罗斯韦尔公园纪念研究所(Roswell Park Memorial Institute,RPMI)开发出来的,RPMI是该研究所开发的一类细胞培养基,1640是培养基代号。RPMI-1640是改进型的McCoy's 5A培养基,使用碳酸氢盐缓冲系统,与大多数哺乳动物细胞培养基不同的是其典型的PH8的配方。RPMI-1640培养基最初是为淋巴细胞培养专门设计的,现在已广泛应用于各种正常细胞和癌细胞的培养,尤其是悬浮细胞的培养,是使用最为广泛的培养基之一。
产品说明
产品形态 | 液体 |
产品浓度 | 1× |
产品规格 | 125mL×4 |
培养基成分 | RPMI-1640(PM150110)+10% FBS(164210-50)+1% P/S(PB180120)+1% GlutaMax(PB180419) |
细菌检测 | 阴性 |
真菌检测 | 阴性 |
支原体检测 | 阴性 |
细胞生长实验 | 细胞生长良好,形态正常 |
内毒素含量(EU/mL) | ≤3 |
储存条件 | 2-8℃,避光储存 |
运输条件 | 冰袋冷藏运输 |
有效期 | 6个月 |
注意事项
2. 本产品为全营养组分的培养基,如无特别需要不用额外再添加血清和双抗,可以直接使用。
3. 为保持本产品的最佳使用效果,不宜将其长时间放置于室温或较高的温度环境中。
4. 所有产品请于保质期内使用,超出保质期,必须放弃使用。
5. 本产品只供进一步科研使用,不可应用于临床等其他方面。
参考文献
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Construction and validation of a diagnostic model for cholangiocarcinoma based on tumor-educated platelet RNA expression profiles (2025-01-13)
期刊:Oncologie
DOI:10.1515/oncologie-2024-0520
影响因子 :1.4
引用产品: RPMI-1640完全培养基(含10% FBS) , DMEM高糖 培养基 , 特级胎牛血清
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N6-methyladenosine-modified SENP1, identified by IGF2BP3, is a novel molecular marker in acute myeloid leukemia and aggravates progression by activating AKT signal via de-SUMOylating HDAC2 (2024-05-31)
期刊:Molecular Cancer
DOI:10.1186/s12943-024-02013-y
影响因子 :27.7
引用产品: 293T [HEK-293T] 细胞 , HL-60 细胞 , THP-1 细胞 , NB4 细胞 , DMEM高糖完全培养基(含10% FBS) , IMDM完全培养基(含10% FBS) , RPMI-1640完全培养基(含10% FBS)
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The Scutellaria-Coptis herb couple and its active small-molecule ingredient wogonoside alleviate cytokine storm by regulating the CD39/NLRP3/GSDMD signaling pathway (2024-04-07)
期刊:JOURNAL OF ETHNOPHARMACOLOGY
影响因子 :5.4
引用产品: RAW 264.7 细胞 , DMEM高糖完全培养基(含10% FBS) , THP-1细胞专用培养基 , RPMI-1640完全培养基(含10% FBS) , RAW 264.7细胞专用培养基 , THP-1 细胞
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Mechanism of norcantharidin intervention in gastric cancer: analysis based on antitumor proprietary Chinese medicine database, network pharmacology, and transcriptomics (2024-09-17)
期刊:Chinese Medicine
DOI:10.1186/s13020-024-01000-1
影响因子 :5.3
引用产品: RPMI-1640完全培养基(含10% FBS)
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Hsa_circRNA_000166 accelerates breast cancer progression via the regulation of the miR-326/ELK1 and miR-330-5p/ELK1 axes (2024-11-12)
期刊:ANNALS OF MEDICINE
DOI:10.1080/07853890.2024.2424515
影响因子 :4.9
引用产品: 特级胎牛血清 , RPMI-1640完全培养基(含10% FBS)
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Glabridin inhibits proliferation and migration in hepatocellular carcinoma by regulating multi-targets (2024-11-06)
期刊:JOURNAL OF ETHNOPHARMACOLOGY
影响因子 :4.8
引用产品: RPMI-1640完全培养基(含10% FBS) , HuH-7 细胞 , Hep G2 细胞 , HuH-7细胞专用培养基
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Artesunate-loaded solid lipid nanoparticles resist esophageal squamous cell carcinoma by inducing Ferroptosis through inhibiting the AKT/mTOR signaling (2024-02-16)
期刊:CELLULAR SIGNALLING
DOI:10.1016/j.cellsig.2024.111108
影响因子 :4.8
引用产品: KYSE-150 细胞 , KYSE-30 细胞 , 人食管上皮细胞 , RPMI-1640完全培养基 (含10%FBS)
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Research on the potential mechanism of Deapioplatycodin D against pulmonary fibrosis based on bioinformatics and experimental verification (2024-04-26)
期刊:EUROPEAN JOURNAL OF PHARMACOLOGY
DOI:10.1016/j.ejphar.2024.176603
影响因子 :4.2
引用产品: BEAS-2B 细胞 , RPMI-1640完全培养基(含10% FBS)
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Integrative analysis based on the cell cycle-related genes identifies TPX2 as a novel prognostic biomarker associated with tumor immunity in breast cancer (2024-04-19)
期刊:Aging-US
影响因子 :3.9
引用产品: MCF7 [MCF-7]细胞专用培养基 , BT-474 [BT474]细胞专用培养基 , T-47D细胞专用培养基 , MDA-MB-453细胞专用培养基(L15) , MDA-MB-231/GFP细胞专用培养基(L15) , MCF 10A细胞专用培养基 , RPMI-1640完全培养基(含10% FBS)
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Role and mechanism of COL3A1 in regulating the growth, metastasis, and drug sensitivity in cisplatin-resistant non-small cell lung cancer cells (2024-03-26)
期刊:CANCER BIOLOGY & THERAPY
DOI:10.1080/15384047.2024.2328382
影响因子 :3.6
引用产品: A549 [A-549] 细胞 , NCI-H460 [H460] 细胞 , Ham's F-12K完全培养基 (含10%FBS) , RPMI-1640完全培养基 (含10%FBS) , HBE cell , A549/DDP 细胞
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The combination of SHOX2 and RASSF1A DNA methylation had a diagnostic value in pulmonary nodules and early lung cancer (2024-01-23)
期刊:ONCOLOGY
影响因子 :3.5
引用产品: DMEM低糖 (含双抗) 培养基 , RPMI-1640完全培养基 (含10%FBS) , Ham's F-12K完全培养基 (含10%FBS)
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PLOD1 promote proliferation and migration with glycolysis via the Wnt/β-catenin pathway in THCA (2024-10-16)
期刊:GENOMICS
DOI:10.1016/j.ygeno.2024.110943
影响因子 :3.4
引用产品: RPMI-1640完全培养基(含10% FBS) , DMEM低糖完全培养基(含10% FBS) , Nthy-ori 3-1 细胞 , DMEM低糖完全培养基(含20% FBS)
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Hsa_circ_0051908 Promotes Hepatocellular Carcinoma Progression by Regulating the Epithelial–Mesenchymal Transition Process (2024-04-30)
期刊:Analytical Cellular Pathology
影响因子 :3.2
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SIRT5 suppresses the trophoblast cell proliferation, invasion, and migration to promote preeclampsia via desuccinylating HOXB3 (2024-08-15)
期刊:JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
DOI:10.1007/s10815-024-03223-5
影响因子 :3.2
引用产品: 青霉素-链霉素溶液(双抗),100× , HTR-8-Svneo 细胞 , RPMI-1640 完全培养基(含10% FBS)
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SPI1-mediated transcriptional activation of CEP55 promotes the malignant growth of triple-negative breast cancer and M2 macrophage polarization (2024-08-13)
期刊:PATHOLOGY RESEARCH AND PRACTICE
影响因子 :2.9
引用产品: RPMI-1640 完全培养基(含10% FBS) , MEM完全培养基(含10% FBS) , DMEM/F12 培养基 , Leibovitz's L-15 培养基 , MEM 培养基
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AMPK-mTOR pathway modulates glycolysis reprogramming in unexplained recurrent spontaneous abortion (2024-12-20)
期刊:BMC Pregnancy and Childbirth
DOI:10.1186/s12884-024-07054-1
影响因子 :2.8
引用产品: RPMI-1640完全培养基(含10% FBS)
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Lathyrol reduces the RCC invasion and incidence of EMT via affecting the expression of AR and SPHK2 in RCC mice (2024-07-04)
期刊:Discover Oncology
DOI:10.1007/s12672-024-01130-w
影响因子 :2.8
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Codonopsis pilosula polysaccharide suppresses the progression of non-small cell lung cancer by triggering NLRP3/GSDMD-dependent pyroptosis (2024-09-30)
期刊:Discover Oncology
DOI:10.1007/s12672-024-01361-x
影响因子 :2.8
引用产品: RPMI-1640完全培养基(含10% FBS)
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CRISPR-mediated WNK4 point mutation aggravates tumor progression and weakens chemotherapy sensitivity in gastric cancer (2024-09-09)
期刊:HISTOLOGY AND HISTOPATHOLOGY
影响因子 :2.5
引用产品: RPMI-1640完全培养基(含10% FBS)
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METTL3 and IGF2BP1-mediated m6A modification of ZHX2 promotes tumor property of renal cell carcinoma (2024-08-19)
期刊:KIDNEY & BLOOD PRESSURE RESEARCH
影响因子 :2.3
引用产品: RPMI-1640 完全培养基(含10% FBS) , 786-O [786-0] 细胞 , OS-RC-2 细胞 , HK-2 细胞 , 特级胎牛血清 , MEM(含NEAA) 培养基 , ACHN 细胞
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Calycosin inhibits triple-negative breast cancer progression through down-regulation of the novel estrogen receptor-α splice variant ER-α30-mediated PI3K/AKT signaling pathway (2023-06-14)
期刊:PHYTOMEDICINE
DOI:10.1016/j.phymed.2023.154924
影响因子 :7.9
引用产品: BT-549 细胞 , MCF 10A 细胞 , RPMI-1640完全培养基 (含10%FBS)
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Dual-inhibition of lactate metabolism and Prussian blue-mediated radical generation for enhanced chemodynamic therapy and antimetastatic effect (2023-04-18)
期刊:Nanoscale
影响因子 :6.7
引用产品: RPMI-1640完全培养基 (含10%FBS) , NCTC clone 929 [L 细胞, L-929] 细胞 , CT26.WT 细胞
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Characterization of anoikis-based molecular heterogeneity in pancreatic cancer and pancreatic neuroendocrine tumor and its association with tumor immune microenvironment and metabolic remodeling (2023-05-10)
期刊:Frontiers in Endocrinology
DOI:10.3389/fendo.2023.1153909
影响因子 :5.2
引用产品: MIA PaCa-2 细胞 , IMDM完全培养基 (含10%FBS) , RPMI-1640完全培养基 (含10%FBS)
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Downregulation of TAB182 promotes cancer stem-like cell properties and therapeutic resistance in triple-negative breast cancer cells (2023-11-13)
期刊:BMC CANCER
DOI:10.1186/s12885-023-11552-4
影响因子 :3.8
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Identification of immunotherapy-related lncRNA signature for predicting prognosis, immunotherapy responses and drug candidates in bladder cancer (2023-04-18)
期刊:BMC CANCER
DOI:10.1186/s12885-023-10828-z
影响因子 :3.8
引用产品: RPMI-1640完全培养基(含10% FBS)
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Decreasing REDD1 expression protects against high glucose-induced apoptosis, oxidative stress and inflammatory injury in podocytes through regulation of the AKT/GSK-3β/Nrf2 pathway (2023-03-07)
期刊:IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
DOI:10.1080/08923973.2023.2183351
影响因子 :3.3
引用产品: RPMI-1640完全培养基 (含10%FBS)
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Dendritic cells infected with recombinant adenoviral vector encoding mouse fibroblast activation protein-α and human livin α exert an antitumor effect against Lewis lung carcinoma in mice (2023-09-27)
期刊:Immunity Inflammation and Disease
影响因子 :3.2
引用产品: 293 [HEK-293] 细胞 , MEM(ATCC改良) 培养基 , 青霉素-链霉素溶液(双抗),100× , RPMI-1640完全培养基(含10% FBS) , 小鼠脾淋巴细胞完全培养基
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Neferine inhibits the progression of diabetic nephropathy by modulating the miR-17-5p/nuclear factor E2-related factor 2 axis (2023-12-04)
期刊:Journal of Traditional Chinese Medicine
DOI:10.19852/j.cnki.jtcm.20231204.004
影响因子 :2.6
引用产品: 青霉素-链霉素溶液(双抗),100× , RPMI-1640完全培养基(含10% FBS) , 人肾小球系膜细胞
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CCT6A promotes esophageal squamous cell carcinoma cell proliferation, invasion and epithelial-mesenchymal transition by activating TGF-β/Smad/c-Myc pathway (2023-04-05)
期刊:IRISH JOURNAL OF MEDICAL SCIENCE
DOI:10.1007/s11845-023-03357-y
影响因子 :2.1
引用产品: TE-1 细胞 , RPMI-1640完全培养基 (含10%FBS)
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Exosomes Promote Atherosclerosis Progression by Regulating Circ_100696/miR-503-5p/PAPPA Axis-Mediated Vascular Smooth Muscle Cells Proliferation and Migration (2023-09-30)
期刊:International Heart Journal
影响因子 :1.5
引用产品: THP-1 细胞 , RPMI-1640完全培养基 (含10%FBS) , β-巯基乙醇 (55mM),1000× , 青霉素-链霉素溶液 (双抗),100× , T/G HA-VSMC 细胞 , T/G HA-VSMC细胞专用培养基
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Water-extracted Lonicera japonica polysaccharide attenuates allergic rhinitis by regulating NLRP3-IL-17 signaling axis (2022-09-03)
期刊:CARBOHYDRATE POLYMERS
DOI:10.1016/j.carbpol.2022.120053
影响因子 :10.7
引用产品: THP-1 细胞 , RPMI-1640完全培养基 (含10%FBS) , β-巯基乙醇 (55mM),1000× , 青霉素-链霉素溶液 (双抗),100× , 特级胎牛血清
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Oral Cancer Stem Cell-Derived Small Extracellular Vesicles Promote M2 Macrophage Polarization and Suppress CD4+ T-Cell Activity by Transferring UCA1 and Targeting LAMC2 (2022-11-28)
期刊:Stem Cells International
影响因子 :5.1
引用产品: CAL 27 细胞 , 293T [HEK-293T] 细胞 , DMEM高糖 (含双抗) 培养基 , THP-1 细胞 , RPMI-1640完全培养基 (含10%FBS)
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Circ_0003159 upregulates LIFR expression through competitively binding to miR-221-3p/miR-222-3p to block gastric cancer development (2022-01-16)
期刊:Journal Of Molecular Histology
DOI:10.1007/s10735-021-10044-8
影响因子 :3.2
引用产品: AGS 细胞 , HGC-27 细胞 , Ham's F-12完全培养基 (含10%FBS) , RPMI-1640完全培养基 (含10%FBS)
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Circ_0044516 Enriches the Level of SARM1 as a miR-330-5p Sponge to Regulate Cell Malignant Behaviors and Tumorigenesis of Prostate Cancer (2022-01-07)
期刊:Biochemical Genetics
DOI:10.1007/s10528-021-10160-w
影响因子 :2.4
引用产品: DU 145 细胞 , LNCaP clone FGC 细胞 , DMEM高糖完全培养基 (含10%FBS) , RPMI-1640完全培养基 (含10%FBS) , RWPE-1 细胞
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CircFBXW8 Acts an Oncogenic Role in the Malignant Progression of Non-small Cell Lung Carcinoma by miR-370-3p-Dependent Regulation of TRIM44 (2022-01-06)
期刊:Biochemical Genetics
DOI:10.1007/s10528-021-10177-1
影响因子 :2.4
引用产品: RPMI-1640完全培养基 (含10%FBS) , DMEM/F12完全培养基 (含10%FBS) , 青霉素-链霉素溶液 (双抗),100×
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The antitumor activity and pharmacokinetics research of PPD-Arg (Tos) using ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (2022-10-26)
期刊:BIOMEDICAL CHROMATOGRAPHY
DOI:10.1002/bmc.5535
影响因子 :1.9
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Network pharmacology and in vitro studies reveal the pharmacological effects and molecular mechanisms of Shenzhi Jiannao prescription against vascular dementia (2022-02-02)
期刊:BMC Complementary and Alternative Medicine
DOI:10.1186/s12906-021-03465-1
影响因子 :0.0
引用产品: RPMI-1640完全培养基 (含10%FBS)
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ADMSC Exo-MicroRNA-22 improve neurological function and neuroinflammation in mice with Alzheimers disease (2021-07-26)
期刊:Journal Of Cellular And Molecular Medicine
影响因子 :5.3
引用产品: PC-12 (High differentiation) 细胞 , RPMI-1640完全培养基 (含10%FBS)
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Exosomal transfer of activated neutrophil-derived lncRNA CRNDE promotes proliferation and migration of airway smooth muscle cells in asthma (2021-09-30)
期刊:Human Molecular Genetics
影响因子 :3.5
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Long non?coding RNA HCG18 facilitates the progression of laryngeal and hypopharyngeal squamous cell carcinoma by upregulating FGFR1 via miR?133b (2021-12-08)
期刊:Molecular Medicine Reports
影响因子 :3.4
引用产品: RPMI-1640完全培养基 (含10%FBS) , 特级胎牛血清 , 青霉素-链霉素溶液 (双抗),100×
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Sevoflurane inhibits malignant progression of colorectal cancer via hsa_circ_0000231-mediated miR-622 (2021-06-28)
期刊:Journal of Biological Research-Thessaloniki
DOI:10.1186/s40709-021-00145-6
影响因子 :3.3
引用产品: SW620 细胞 , HCT 116 细胞 , 磷酸盐缓冲液 (PBS) 1× , RPMI-1640完全培养基 (含10%FBS) , SW620细胞专用培养基 (L15)
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LINC00261 Inhibits Esophageal Cancer Radioresistance by Down-Regulating microRNA-552-3p and Promoting DIRAS1 (2021-11-13)
期刊:Cancer Management and Research
影响因子 :3.3
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LncRNA NEAT1 Knockdown Alleviates Lipopolysaccharide-Induced Acute Lung Injury by Modulation of miR-182-5p/WISP1 Axis (2021-05-27)
期刊:Biochemical Genetics
DOI:10.1007/s10528-021-10081-8
影响因子 :2.4
引用产品: MH-S 细胞 , RPMI-1640完全培养基 (含10%FBS)
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Circ_0007031 Silencing Inhibits Cell Proliferation and Induces Cell Apoptosis via Downregulating MELK at a miR-485-3p-Dependent Way in Colorectal Cancer (2021-07-28)
期刊:Biochemical Genetics
DOI:10.1007/s10528-021-10111-5
影响因子 :2.4
引用产品: HCT 116 细胞 , RPMI-1640完全培养基 (含10%FBS)
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BIOINFORMATIC ANALYSIS OF DOSE- AND TIME-DEPENDENT miRNome RESPONSES (2019-05-01)
期刊:Radiation Protection Dosimetry
影响因子 :1.0
引用产品: VE 细胞 , RPMI-1640完全培养基 (含10%FBS)
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A pH-responsive organic photosensitizer specifically activated by cancer lysosomes (2018-04-08)
期刊:Dyes And Pigments
DOI:10.1016/j.dyepig.2018.04.008
影响因子 :4.5
引用产品: RPMI-1640完全培养基 (含10%FBS)
FAQs
Q:完培怎么复温?
A:放室温复温就可以呢,从冰箱提前拿出复温半小时到一个小时,手摸不冰就可以正常使用了。
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