产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
产品概述
| 名称 | SW 1353 (人软骨肉瘤细胞) (STR鉴定正确) |
| 别称 | SW-1353; SW 1353 |
| 种属 | 人类 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，100%, 温度：37℃
|
| 培养方案B（可选） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 1.该细胞推荐使用Leibovitz's L-15培养基进行培养，Leibovitz's L-15不可以通入二氧化碳，会产生细胞毒性；2.如您没有无二氧化碳的培养箱，可使用DMEM替代Leibovitz's L-15，使用DMEM培养基时即可正常通入5%二氧化碳；3.配套专用培养基默认Leibovitz's L-15配置，如需DMEM配方，请联系销售下单备注更改。 |
| 背景描述 | SW 1353细胞最初从一位72岁女性白人的右肱骨原发性Ⅱ级软骨肉瘤中分离得到。最初的培养基是含有可的松和胰岛素的L-15培养基添加10%FBS和1%抗生素。1982年1月，ATCC收到１支传代至12代的冻存管SW 1353细胞。 |
| 年龄（性别） | 女性；72岁 |
| 组织来源 | 骨；软骨肉瘤 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肉瘤细胞 |
| 生物安全等级 | BSL-1 |
| 抗原表达情况 | Blood Type O- |
| 保藏机构 | ATCC; HTB-94 |
STR鉴定
-
STR位点信息
Amelogenin X CSF1PO 12 D2S1338 19,24 D3S1358 15,18 D5S818 10,11 D7S820 9,11 D8S1179 10,11 D13S317 12,13 D16S539 11,12 D18S51 13,17 D19S433 14 D21S11 30,32.2 FGA 22,23 PentaD 10,11 PentaE 12,14 TH01 6,9 TPOX 8,11 vWA 16,17 D6S1043 12,14 D12S391 22,23 D2S441 11,14 -
STR鉴定图
-
参考文献
-
A biosensor for D-2-hydroxyglutarate in frozen sections and intraoperative assessment of IDH mutation status (2023-12-12)
作者：Wen Zhang:1.Institute of Medical Sciences, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, People's Republic of China. ; Mu Yang:1.Institute of Medical Sciences, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, People's Republic of China. ; Gang Wang:1.Department of Neurosurgery, The First Hospital of China Medical University, Shenyang, People's Republic of China. ; Shaowu Ou:1.Department of Neurosurgery, The First Hospital of China Medical University, Shenyang, People's Republic of China. ; Jinqu Hu:1.Department of Neurosurgery, The First Hospital of China Medical University, Shenyang, People's Republic of China. ; Jiyuan Liu:1.Department of Neurosurgery, The First Hospital of China Medical University, Shenyang, People's Republic of China. ; Yuxin Lei:1.Institute of Medical Sciences, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, People's Republic of China. ; Zhaoqi Kang:1.State Key Labora
期刊：A biosensor for D-2-hydroxyglutarate in frozen sections and intraoperative assessment of IDH mutation status
DOI：10.1016/j.bios.2023.115921
影响因子 ：12.6
引用产品： HT-1080 [HT1080] 细胞 , SW 1353 细胞 , U-87 MG 细胞 , U-87 MG细胞专用培养基
-
Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments (2023-06-01)
作者：Zhiwei Zhang:1.Department of Orthopedics, The First Affiliated Hospital of Nanchang University, Artificial Joints Engineering and Technology Research Center of Jiangxi Province, Nanchang 330006, China. ; Song Wang:1.Department of Orthopedics, The First Affiliated Hospital of Nanchang University, Artificial Joints Engineering and Technology Research Center of Jiangxi Province, Nanchang 330006, China. ; Xuqiang Liu:1.Department of Orthopedics, The First Affiliated Hospital of Nanchang University, Artificial Joints Engineering and Technology Research Center of Jiangxi Province, Nanchang 330006, China. ; Yuxin Yang:1.Huankui academy, Nanchang University, Nanchang 330006, China. ; Yiqin Zhang:1.Huankui academy, Nanchang University, Nanchang 330006, China. ; Bo Li:1.Department of Orthopedics, The First Affiliated Hospital of Nanchang University, Artificial Joints Engineering and Technology Research Center of Jiangxi Province, Nanchang 330006, China. ; Fengfen Guo:1.Department of Orthop
期刊：Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments
DOI：10.1016/j.biopha.2023.114964
影响因子 ：7.5
引用产品： SW 1353 细胞 , DMEM高糖 培养基
-
MicroRNA PC-3p-2869 Regulates Antler Growth and Inhibits Proliferation and Migration of Human Osteosarcoma and Chondrosarcoma Cells by Targeting CDK8, EEF1A1, and NTN1 (2023-06-29)
作者：Fan Yang:1.Laboratory of Genetics and Molecular Biology, College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China. ; Jin Wu:1.Laboratory of Genetics and Molecular Biology, College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China. ; Mindie Zhao:1.Laboratory of Genetics and Molecular Biology, College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China. ; Han Zheng:1.Biotechnology Program, Division of Biology and Medicine, Brown University, Providence, RI 02912, USA. ; Jingyuan Suo:1.Laboratory of Genetics and Molecular Biology, College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China. ; Xuedong Liu:1.Laboratory of Genetics and Molecular Biology, College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China. ; Dong Zheng:1.Laboratory of Genetics and Molecular Biology, College of Wildlife and Protected Area, Northeast F
期刊：MicroRNA PC-3p-2869 Regulates Antler Growth and Inhibits Proliferation and Migration of Human Osteosarcoma and Chondrosarcoma Cells by Targeting CDK8, EEF1A1, and NTN1
影响因子 ：5.6
引用产品： 293T [HEK-293T] 细胞 , U-2 OS [U2OS] 细胞 , MG-63 细胞 , SW 1353 细胞
-
HTRA3 transcriptionally inhibited by FOXP1 suppresses tumorigenesis of osteosarcoma via the PTEN/PI3K/AKT pathway (2023-07-30)
作者：Ziyuan Tong:1.Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning, People's Republic of China. ; Yuan Shen:1.Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning, People's Republic of China. ; Quan Yuan:1.Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning, People's Republic of China. ; Honghao Yu:1.Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning, People's Republic of China. Electronic address: yhhspine2022@163.com. ;
期刊：HTRA3 transcriptionally inhibited by FOXP1 suppresses tumorigenesis of osteosarcoma via the PTEN/PI3K/AKT pathway
DOI：10.1016/j.bbamcr.2023.119553
影响因子 ：5.1
引用产品： HOS 细胞 , Saos-2 细胞 , SW 1353 细胞 , McCoy's 5A 培养基
-
hsa_circ_0020378 regulating miR-339-3p/COL1A1 promotes osteosarcoma progression (2023-11-18)
作者：Liang Liu:1.Department of Spinal Surgery, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Huangshi, Hubei, China. ; 2.Department of molecular therapy of cancer, Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, Huangshi, Hubei, China. ; Wenze Huang:1.Department of Spinal Surgery, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Huangshi, Hubei, China. ; 2.Department of molecular therapy of cancer, Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, Huangshi, Hubei, China. ;
期刊：hsa_circ_0020378 regulating miR-339-3p/COL1A1 promotes osteosarcoma progression
DOI：10.1080/15384047.2023.2274120
影响因子 ：3.6
引用产品： hFOB 1.19 细胞 , HOS 细胞 , Saos-2 细胞 , SW 1353 细胞 , Leibovitz's L-15 培养基 , McCoy's 5A完全培养基 (含10%FBS） , MEM 培养基 , DMEM/F12 培养基 , 青霉素-链霉素溶液 (双抗)，100× , 特级胎牛血清
-
ADAM10 promotes cell growth, migration, and invasion in osteosarcoma via regulating E-cadherin/β-catenin signaling pathway and is regulated by miR-122-5p (2003-00-20)
作者：Quan Yuan:1.1Department of Orthopedics, Shengjing Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang, 110004 People's Republic of China. ; Honghao Yu:1.1Department of Orthopedics, Shengjing Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang, 110004 People's Republic of China. ; Jianhua Chen:1.1Department of Orthopedics, Shengjing Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang, 110004 People's Republic of China. ; Xiaoyu Song:1.2Institute of Translational Medicine, China Medical University, Shenyang, 110122 People's Republic of China. ; Li Sun:1.3Department of Nephrology, The First Hospital of China Medical University, Shenyang, 110001 People's Republic of China. ;
期刊：ADAM10 promotes cell growth, migration, and invasion in osteosarcoma via regulating E-cadherin/β-catenin signaling pathway and is regulated by miR-122-5p
DOI：10.1186/s12935-020-01174-2
影响因子 ：5.8
引用产品： Saos-2 细胞 , SW 1353 细胞 , HOS 细胞 , McCoy's 5A 培养基
FAQs
Q：{{item.question}}
A：
产品资料
识别码示意图
