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产品概述
人牙龈成纤维细胞
Cat NO.: CP-H240
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产品名称：人牙龈成纤维细胞
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组织来源：牙龈组织
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产品规格：5×10⁵Cells/T25培养瓶
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细胞简介：
人牙龈成纤维细胞分离自牙龈组织；牙龈指紧贴于牙颈周围及邻近的牙槽骨上淡红色的结构，由复层扁平上皮及固有层组成。是口腔黏膜的一部分，血管丰富，呈淡红色，坚韧而有弹性，因缺乏黏膜下层，直接与骨膜紧密相连，故牙龈不能移动。成纤维细胞(Fibroblast)是疏松结缔组织的主要细胞成分，由胚胎时期的间充质细胞分化而来。成纤维细胞较大，轮廓清楚，多为突起的纺锤形或星形的扁平状结构，其细胞核呈规则的卵圆形，核仁大而明显。成纤维细胞功能活动旺盛，细胞质嗜弱碱性，具明显的蛋白质合成和分泌活动，在一定条件下，它可以实现跟纤维细胞的互相转化；成纤维细胞对不同程度的细胞变性、坏死和组织缺损的修复有着十分重要的作用。刚分离的表皮成纤维细胞呈圆形、折光性良好，悬浮于培养基中。30min细胞贴壁，其中部分开始伸出伪足，表现为小的突起；6h后细胞基本贴壁完全，伸展成梭形，胞核清晰，分布较均匀，散在生长，不聚集成团；细胞生长迅速，5-7天即呈融合状态，细胞排列紧密，有的交叉重叠生长，平坦、胞体较大，细胞质透明，细胞核较大，呈椭圆形，颜色淡。细胞融合，并彼此连接成网状；细胞呈突起的纺锤形或星形的扁平分布。
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方法简介：
普诺赛实验室分离的人牙龈成纤维细胞采用胰蛋白酶 - 胶原酶混合酶消化后差速贴壁制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人牙龈成纤维细胞经Vimentin免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H240 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传3-5代左右 消化液 0.25%胰蛋白酶 人牙龈成纤维细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma Cells (2024-01-09)
作者：Qiaozhen Zhou:1.Department (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, China. ; Qianqian Zhang:1.Department (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, China. ; Lingzi Liao:1.Department (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, China. ; Qian Li:1.Department (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, China. ; Huidan Qu:1.Department (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, China. ; Xinyu Wang:1.Department (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, China. ; Ying Zhou:1.Department (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, China. ; Guangzeng Zhang:1.Department (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, China. ; Mingliang Sun:1.Department (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, China. ; Kailiang Zhang:1.Department (Hospital) of Stomatology, Lanzhou University, Lanzhou 730000, China. ; 2.Key Laboratory
期刊：Isocorydine Exerts Anticancer Activity by Disrupting the Energy Metabolism and Filamentous Actin Structures of Oral Squamous Carcinoma Cells
影响因子 ：3.1
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Effects of Sulforaphene on the Cariogenic Properties of Streptococcus Mutans In Vitro and Dental Caries Development In Vivo (2023-08-24)
作者：Yuehong Zhou:1.The College of Renji, Wenzhou Medical University, Wenzhou 325000, China. ; Binhan Zhang:1.State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. ; 2.The College of Life Science, Sichuan University, Chengdu 610041, China. ; Yufei Wang:1.State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. ; Rongdang Hu:1.The College of Renji, Wenzhou Medical University, Wenzhou 325000, China. ;
期刊：Effects of Sulforaphene on the Cariogenic Properties of Streptococcus Mutans In Vitro and Dental Caries Development In Vivo
DOI：10.3390/antibiotics12091359
影响因子 ：4.8
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Fibronectin-coated microgroove titanium surface promotes the behaviors of human gingival fibroblasts (2023-04-13)
作者：Sijia Huang:1.Quanzhou Medical College. ; Yanjun Lin:1.School and Hospital of Stomatology, Fujian Medical University. ; Lin Zhou:1.School and Hospital of Stomatology, Fujian Medical University. ;
期刊：Fibronectin-coated microgroove titanium surface promotes the behaviors of human gingival fibroblasts
影响因子 ：2.5
引用产品： 人牙龈成纤维细胞
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Antibacterial Properties of Small-Size Peptide Derived from Penetratin against Oral Streptococci (2005-01-20)
作者：Meng Li:1.School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin 300070, China. ; Yanyan Yang:1.School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin 300070, China. ; 2.Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou 510150, China. ; Chen Lin:1.School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin 300070, China. ; 2.Fengtai Maternal & Child Health Hospital, Beijing 100069, China. ; Qian Zhang:1.School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin 300070, China. ; Lei Gong:1.Department of Esophageal Cancer, Tianjin's Clinical Research Center for Cancer and Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300070, China. ; Yonglan Wang:1.School and Hospital of Stomatology, Tianjin Medical Unive
期刊：Antibacterial Properties of Small-Size Peptide Derived from Penetratin against Oral Streptococci
影响因子 ：3.4
引用产品： 人牙龈成纤维细胞
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