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产品概述
人口腔上皮细胞
Cat NO.: CP-H203
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产品名称:人口腔上皮细胞
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组织来源:口腔黏膜组织
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产品规格:5×10⁵Cells/T25培养瓶
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细胞简介:
人口腔上皮细胞分离自口腔黏膜组织;口腔上皮细胞是一种上皮细胞,呈扁平、多边形,形状不规则。口腔各壁都有黏膜覆盖,口腔上皮细胞主要分布在口腔两侧颊部。上皮的垂直切面上,细胞形状不一。紧靠基膜的一层基底细胞为矮柱状,为具有增殖分化能力的干细胞,部分子细胞向浅层移动。基底层以上是数层多边形细胞,再上为几层梭形或扁平细胞。仅靠近表面几层细胞为扁平状,基底层细胞能不断分裂增生,以补充表层衰老或损伤脱落的细胞。复层扁平上皮深层的结缔组织内有丰富的毛细血管,有利于复层扁平上皮的营养。
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方法简介:
普诺赛实验室分离的人口腔上皮细胞采用胰蛋白酶-胶原酶混合消化法结合差速贴壁法,并通过上皮细胞专用培养基培养筛选制备而来,细胞总量约为5×10⁵cells/瓶。
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质量检测:
普诺赛实验室分离的人口腔上皮细胞经Cytokeratin-18免疫荧光鉴定,纯度可达90%以上,且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息:
包被条件 鼠尾胶原Ⅰ(2-5μg/cm2) 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H203 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 上皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 人口腔上皮细胞体外培养周期有限;建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养,以此保证该细胞的最佳培养状态。
参考文献
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Anti-Wound Dehiscence and Antibacterial Dressing with Highly Efficient Self-Healing Feature for Guided Bone Regeneration Wound Closure (2024-02-27)
作者:Shenghao Xue:1.Department of Prothodontics, Shanghai Stomatological Hospital & School of Stomatology, Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, 200001, P. R. China. ; Ning Tang:1.Precision Research Center for Refractory Diseases in Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China. ; Cheng Zhou:1.School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, P. R. China. ; Shuobo Fang:1.Department of Prothodontics, Shanghai Stomatological Hospital & School of Stomatology, Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, 200001, P. R. China. ; Hossam Haick:1.Department of Chemical Engineering and Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology, Haifa, 3200003, Israel. ; Jiao Sun:1.Department of Dental Materials, Shanghai NinthPeople's Hospital,
期刊:Anti-Wound Dehiscence and Antibacterial Dressing with Highly Efficient Self-Healing Feature for Guided Bone Regeneration Wound Closure
影响因子 :10.0
引用产品: 人口腔上皮细胞
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Promotional Effect and Molecular Mechanism of Synthesized Zinc Oxide Nanocrystal on Zirconia Abutment Surface for Soft Tissue Sealing (2023-03-08)
作者:J Hu:1.Beijing Stomatological Hospital, Capital Medical University, Beijing, China. ; I Atsuta:1.Division of Advanced Dental Devices and Therapeutics, Faculty of Dental Science, Kyushu University, Fukuoka, Japan. ; Y Luo:1.Beijing Luhe Hospital, Capital Medical University, Beijing, China. ; X Wang:1.Beijing Stomatological Hospital, Capital Medical University, Beijing, China. ; Q Jiang:1.Beijing Stomatological Hospital, Capital Medical University, Beijing, China. ;
期刊:Promotional Effect and Molecular Mechanism of Synthesized Zinc Oxide Nanocrystal on Zirconia Abutment Surface for Soft Tissue Sealing
影响因子 :7.6
引用产品: 人口腔上皮细胞
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Effects of Sulforaphene on the Cariogenic Properties of Streptococcus Mutans In Vitro and Dental Caries Development In Vivo (2023-08-24)
作者:Yuehong Zhou:1.The College of Renji, Wenzhou Medical University, Wenzhou 325000, China. ; Binhan Zhang:1.State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. ; 2.The College of Life Science, Sichuan University, Chengdu 610041, China. ; Yufei Wang:1.State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China. ; Rongdang Hu:1.The College of Renji, Wenzhou Medical University, Wenzhou 325000, China. ;
期刊:Effects of Sulforaphene on the Cariogenic Properties of Streptococcus Mutans In Vitro and Dental Caries Development In Vivo
DOI:10.3390/antibiotics12091359
影响因子 :4.8
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ACTN1 interacts with ITGA5 to promote cell proliferation, invasion and epithelial-mesenchymal transformation in head and neck squamous cell carcinoma (2023-02-01)
作者:Rui Wang:1.Department of Otolaryngology-Head and Neck Surgery, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030032, China. ; 2.Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. ; Ying Gao:1.Department of Otolaryngology-Head and Neck Surgery, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030032, China. ; 2.Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. ; Huimin Zhang:1.Department of Otolaryngology-Head and Neck Surgery, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030032, China. ; 2.Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technol
期刊:ACTN1 interacts with ITGA5 to promote cell proliferation, invasion and epithelial-mesenchymal transformation in head and neck squamous cell carcinoma
DOI:10.22038/IJBMS.2022.67056.14703
影响因子 :2.2
引用产品: 人口腔上皮细胞 , CAL 27 细胞 , SCC-9 [SCC 9; SCC9] 细胞
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Interleukin-6 mediated inflammasome activation promotes oral squamous cell carcinoma progression via JAK2/STAT3/Sox4/NLRP3 signaling pathway (2005-02-20)
作者:Li Xiao:1.Department of Stomatology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, Sichuan, China. ; Xue Li:1.Department of Stomatology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, Sichuan, China. ; Peilin Cao:1.Department of Stomatology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, Sichuan, China. ; Wei Fei:1.Department of Stomatology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, Sichuan, China. ; Hao Zhou:1.Department of Stomatology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, Sichuan, China. ; Na Tang:1.Department of Stomatology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, Si
期刊:Interleukin-6 mediated inflammasome activation promotes oral squamous cell carcinoma progression via JAK2/STAT3/Sox4/NLRP3 signaling pathway
DOI:10.1186/s13046-022-02376-4
影响因子 :11.3
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Procyanidin B2 inhibits angiogenesis and cell growth in oral squamous cell carcinoma cells through the vascular endothelial growth factor (VEGF)/VEGF receptor 2 (VEGFR2) pathway (2002-02-20)
作者:Qiurong Sun:1.Department of Stomatology, The First Affiliated Hosital of Gannan Medical University, Ganzhou, Jiangxi, China. ; Taiyang Zhang:1.Department of Stomatology, The First Affiliated Hosital of Gannan Medical University, Ganzhou, Jiangxi, China. ; Qingchun Xiao:1.Department of Stomatology, The First Affiliated Hosital of Gannan Medical University, Ganzhou, Jiangxi, China. ; Bingxin Mei:1.Department of Stomatology, The First Affiliated Hosital of Gannan Medical University, Ganzhou, Jiangxi, China. ; Xingwang Zhang:1.Department of Oral Surgery, Daqing Longnan Hospital, Daqing, Heilongjiang, China. ;
期刊:Procyanidin B2 inhibits angiogenesis and cell growth in oral squamous cell carcinoma cells through the vascular endothelial growth factor (VEGF)/VEGF receptor 2 (VEGFR2) pathway
DOI:10.1080/21655979.2022.2033013
影响因子 :4.9
引用产品: 人口腔上皮细胞 , 人口腔上皮细胞完全培养基
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Hsa_circRNA_0001971 contributes to oral squamous cell carcinoma progression via miR-186-5p/Fibronectin type III domain containing 3B axis (2001-02-20)
作者:Jiehua Zhang:1.Department of Stomatology, Renmin Hospital of Wuhan University, Wuhan, China. ; 2.Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan, China. ; Youjian Peng:1.Department of Stomatology, Renmin Hospital of Wuhan University, Wuhan, China. ; Shengjun Jiang:1.Department of Stomatology, Renmin Hospital of Wuhan University, Wuhan, China. ; Jun Li:1.Department of Stomatology, Renmin Hospital of Wuhan University, Wuhan, China. ;
期刊:Hsa_circRNA_0001971 contributes to oral squamous cell carcinoma progression via miR-186-5p/Fibronectin type III domain containing 3B axis
影响因子 :2.7
引用产品: 人口腔上皮细胞
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