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产品概述
名称 | RPMI 8226 (人多发性骨髓瘤外周血B淋巴细胞) (STR鉴定正确) |
别称 | RPMI 8226; RPMI.8226; RPMI8226; RPMI no 8226; 8226; RPMI 8226/S; RPMI-8226S; RPMI8226/S; 8226/S; Roswell Park Memorial Institute 8226; GM02132; GM2132; GM 2132; Simpson |
种属 | 人 |
生长特性 | 悬浮细胞 |
细胞形态 | 淋巴母细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 5-8×10^5 cells/mL |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。 |
背景描述 | 没有证据表明,RPMI 8226细胞能产生重链(细胞质型或分泌型)。 |
年龄(性别) | 男性;61岁 |
组织来源 | 外周血,B淋巴细胞 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 骨髓瘤细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~60 hours |
保藏机构 | ATCC; CCL-155 ATCC; CRM-CCL-155 DSMZ; ACC-402 ECACC; 87012702 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 12 D2S1338 20 D3S1358 16,17 D5S818 11,13 D7S820 9,10 D8S1179 13 D13S317 11 D16S539 9 D18S51 15,19 D19S433 13.2,14 D21S11 28,29 FGA 19 PentaD 2.2,11 PentaE 16 TH01 8 TPOX 8,11 vWA 16,18 D6S1043 14,18 D12S391 16,19 D2S441 12,14 -
STR鉴定图
参考文献
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Targeting Senescent Alveolar Epithelial Cells Using Engineered Mesenchymal Stem Cell-Derived Extracellular Vesicles To Treat Pulmonary Fibrosis (2024-02-21)
作者:Yaoying Long:1.Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. ; Bianlei Yang:1.Department of Rheumatology and Immunology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. ; Qian Lei:1.West China Biomedical Big Data Center, West China Hospital, Sichuan University, Chengdu 610041, China. ; Fei Gao:1.Department of Hematology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China. ; Li Chen:1.Department of Hematology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China. ; Wenlan Chen:1.Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. ; Siyi Chen:1.Department of Rheumatology and Immunology, Union Hospital, Tongji Medical C
期刊:Targeting Senescent Alveolar Epithelial Cells Using Engineered Mesenchymal Stem Cell-Derived Extracellular Vesicles To Treat Pulmonary Fibrosis
影响因子 :17.1
引用产品: A549 [A-549] 细胞 , RAW 264.7 细胞 , RPMI 8226 细胞
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Ethanol Extract of Eclipta prostrata induces Multiple Myeloma Ferroptosis via Keap1/Nrf2/HO-1 Axis (2024-02-01)
作者:Wenxia Li:1.Hangzhou Innovation Institute, Beihang University, Hangzhou, Zhejiang, PR China; Department of Hematology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, PR China. ; Xuejiao Yin:1.Department of Hematology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, PR China. ; Hangjie Fu:1.School of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, PR China; College of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, PR China. ; Jinyuan Liu:1.College of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, PR China. ; Zhiwei Weng:1.College of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, PR China. ; Qingqing Mao:1.College of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, PR China. ; Lijian Zhu:1.College of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, PR China.
期刊:Ethanol Extract of Eclipta prostrata induces Multiple Myeloma Ferroptosis via Keap1/Nrf2/HO-1 Axis
DOI:10.1016/j.phymed.2024.155401
影响因子 :7.9
引用产品: RPMI 8226 细胞 , U266 [U266B1] 细胞
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Design, synthesis, and in vitro biological evaluation of novel HDAC inhibitors bearing C-1 phenyl substituted tetrahydroisoquinoline Cap moiety as anti-tumor therapeutic agents (2024-03-27)
作者:Jie Wang: 1.Department of Chemistry, BengBu Medical College, Bengbu, 233030, PR China; Chi Zhou: 1.Department of Chemistry, BengBu Medical College, Bengbu, 233030, PR China; Bo Li: 1.Department of Chemistry, BengBu Medical College, Bengbu, 233030, PR China; Huaqing Liu: 1.Department of Chemistry, BengBu Medical College, Bengbu, 233030, PR China; Hui Zhang: 1.Department of Chemistry, BengBu Medical College, Bengbu, 233030, PR China; Lei Liu: 1.Department of Chemistry, BengBu Medical College, Bengbu, 233030, PR China;
期刊:Design, synthesis, and in vitro biological evaluation of novel HDAC inhibitors bearing C-1 phenyl substituted tetrahydroisoquinoline Cap moiety as anti-tumor therapeutic agents
DOI:10.1007/s00044-024-03206-2
影响因子 :2.6
引用产品: RPMI 8226 细胞
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LGR4 promotes tumorigenesis by activating TGF-β1/Smad signaling pathway in multiple myeloma (2023-07-18)
作者:Zhigang Yi:1.Department of Hematology, Lanzhou University Second Hospital, Lanzhou, China; Department of Pediatric Orthopedics and Pediatrics, Lanzhou University Second Hospital, Lanzhou, China. ; Tao Ma:1.Department of Hematology, Lanzhou University Second Hospital, Lanzhou, China; Department of Hematology, The Affiliated Hospital of Southwest Medical University, Luzhou, China. ; Jia Liu:1.Department of Hematology, Lanzhou University Second Hospital, Lanzhou, China. ; Wenting Tie:1.Department of Hematology, Lanzhou University Second Hospital, Lanzhou, China. ; Yanhong Li:1.Department of Hematology, Lanzhou University Second Hospital, Lanzhou, China. ; Jun Bai:1.Department of Hematology, Lanzhou University Second Hospital, Lanzhou, China. ; Lijuan Li:1.Department of Hematology, Lanzhou University Second Hospital, Lanzhou, China. Electronic address: doctorjuan@sina.com. ; Liansheng Zhang:1.Department of Hematology, Lanzhou University Second Hospital, Lanzhou, China. Electronic ad
期刊:LGR4 promotes tumorigenesis by activating TGF-β1/Smad signaling pathway in multiple myeloma
DOI:10.1016/j.cellsig.2023.110814
影响因子 :4.8
引用产品: RPMI 8226 细胞 , AMO-1 细胞 , U266 [U266B1] 细胞
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LAMP5 may promote MM progression by activating p38 (2023-03-22)
作者:Yan Chen:1.Department of Hematology, The Affiliated Hospital of Southwest Medical University, Luzhou, China. ; Tao Ma:1.Department of Hematology, The Affiliated Hospital of Southwest Medical University, Luzhou, China. ;
期刊:LAMP5 may promote MM progression by activating p38
影响因子 :2.8
引用产品: RPMI 8226 细胞 , U266 [U266B1] 细胞 , NCI-H929 细胞 , AMO-1 细胞
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NORAD promotes multiple myeloma cell progression via BMP6/P-ERK1/2 axis (2022-09-17)
作者:Tao Ma:1.Department of Hematology, Lanzhou University Second Hospital, Lanzhou 730000, China; Department of Hematology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China. ; Yan Chen:1.Department of Hematology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China. ; Zhi-Gang Yi:1.Department of Hematology, Lanzhou University Second Hospital, Lanzhou 730000, China. ; Jia Liu:1.Department of Hematology, Lanzhou University Second Hospital, Lanzhou 730000, China. ; Yan-Hong Li:1.Department of Hematology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China. ; Jun Bai:1.Department of Hematology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China. ; Wen-Ting Tie:1.Department of Hematology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China. ; Mei Huang:1.Department of Hematology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
期刊:NORAD promotes multiple myeloma cell progression via BMP6/P-ERK1/2 axis
DOI:10.1016/j.cellsig.2022.110474
影响因子 :4.9
引用产品: RPMI 8226 细胞 , AMO-1 细胞
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Hsa_Circ_0134426 Attenuates the Malignant Biological Behaviors of Multiple Myeloma by Suppressing miR-146b-3p to Upregulate NDNF (2022-12-02)
作者:Menglan Li:1.Department of Hematology, Taikang Tongji (Wuhan)Hospital, No. 322, Sixin North Road, Hanyang District, Wuhan, 430050, Hubei, China. ; Jingyuan Li:1.Department of Hematology, Taikang Tongji (Wuhan)Hospital, No. 322, Sixin North Road, Hanyang District, Wuhan, 430050, Hubei, China. ; Yanyan Song:1.Department of Hematology, Taikang Tongji (Wuhan)Hospital, No. 322, Sixin North Road, Hanyang District, Wuhan, 430050, Hubei, China. yanyansong3@163.com. ;
期刊:Hsa_Circ_0134426 Attenuates the Malignant Biological Behaviors of Multiple Myeloma by Suppressing miR-146b-3p to Upregulate NDNF
DOI:10.1007/s12033-022-00618-6
影响因子 :2.9
引用产品: NCI-H929 细胞 , RPMI 8226 细胞 , U266 [U266B1] 细胞 , Normal Plasma Cells , RPMI-1640 培养基 , 特级胎牛血清
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Stimulus-Responsive Microfluidic Interface Enables Efficient Enrichment and Cytogenetic Profiling of Circulating Myeloma Cells (2003-01-20)
作者:Yilong Liu:1.MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemical of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China. ; Rui Su:1.Department of Hematology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen 361005, China. ; Juan Song:1.MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemical of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China. ; Xiyuan Yu:1.MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemical of Solid Surfaces, Department of Che
期刊:Stimulus-Responsive Microfluidic Interface Enables Efficient Enrichment and Cytogenetic Profiling of Circulating Myeloma Cells
影响因子 :9.5
引用产品: RPMI 8226 细胞
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Quercetin inhibits the proliferation of multiple myeloma cells by upregulating PTPRR expression (2009-01-20)
作者:Houcai Wang:1.Department of Hematology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China. ; Dandan Yu:1.Department of Hematology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China. ; Hui Zhang:1.Department of Hematology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China. ; Ruye Ma:1.Department of Hematology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China. ; Huiqun Wu:1.Department of Hematology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China. ; Huili Zhai:1.Department of Hematology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China. ; Huaping Wang:1.Department of Hematology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China. ; Jingjing Li:1.Department of Hematolog
期刊:Quercetin inhibits the proliferation of multiple myeloma cells by upregulating PTPRR expression
影响因子 :3.5
引用产品: RPMI 8226 细胞 , NCI-H929 细胞
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Circ_SEC61A1 contributes to the progression of multiple myeloma cells via regulating miR-660-5p/CDK6 axis (2012-01-20)
作者:Zimian Luo:1.Department of Hematology, Central Hospital of XiangTan, China. ; Yafei Yin:1.Department of Hematology, Central Hospital of XiangTan, China. ; Xiaojun Tan:1.Fertility & Genetic Center, Central Hospital of XiangTan, China. ; Kang Liu:1.Department of Hematology, Central Hospital of XiangTan, China. ; Zhi Chao:1.Department of Hematology, Central Hospital of XiangTan, China. ; Hong Xia:1.Epartment of Orthopedics, Central Hospital of XiangTan, China. Electronic address: Sunlight2235@126.com. ;
期刊:Circ_SEC61A1 contributes to the progression of multiple myeloma cells via regulating miR-660-5p/CDK6 axis
DOI:10.1016/j.leukres.2021.106774
影响因子 :2.7
引用产品: RPMI 8226 细胞 , U266 [U266B1] 细胞 , RPMI-1640 培养基 , 青霉素-链霉素溶液 (双抗),100× , 特级胎牛血清
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Expression of STOML2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating PAI-1 (2011-01-20)
作者:Hongxia Zhang:1.Department of Hematology, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi City, 832008, Xinjiang Uygur Autonomous Region, China. ; Guangsheng Wu:1.Department of Hematology, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi City, 832008, Xinjiang Uygur Autonomous Region, China. ; Junjian Feng:1.Department of Intensive Care Unit, Luzhou People's Hospital, Luzhou City, 646000, Sichuan Province, China. ; Xiaohong Lu:1.Department of Hematology, Luzhou People's Hospital, Luzhou City, 646000, Sichuan Province, China. ; Ping Liu:1.Department of Pathology, Luzhou People's Hospital, Section 2 of JiuGu Avenue, Jiangyang District, Luzhou City, 646000, Sichuan Province, China. liuping_0901@163.com. ;
期刊:Expression of STOML2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating PAI-1
DOI:10.1186/s13018-021-02819-2
影响因子 :2.6
引用产品: RPMI 8226 细胞 , NCI-H929 细胞 , U266 [U266B1] 细胞 , RPMI-1640 培养基
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Anti-bacterial and anti-viral nanchangmycin displays anti-myeloma activity by targeting Otub1 and c-Maf (2009-00-20)
作者:Yujia Xu:1.Guangdong Institute of Cardiovascular Diseases, Guangdong Key Laboratory of Vascular Diseases, the Second Affiliated Hospital; Guangdong Key Laboratory of Protein Modification and Degradation, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, 511436, P. R. China. ; 2.Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, 215123, P. R. China. ; Tong Sun:1.Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, 215123, P. R. China. ; 2.Department of Neurology, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215100, P. R. China. ; Kun Zeng:1.Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, 215123, P. R. China. ; Min Xu:1.Department of Hematology, Zhangjiagang Hospital of Soochow University, Zhangjiagang, 215620, China. ; Jinhao Chen:1.Department of Hematology, Zhangjiagang Hospital of So
期刊:Anti-bacterial and anti-viral nanchangmycin displays anti-myeloma activity by targeting Otub1 and c-Maf
DOI:10.1038/s41419-020-03017-4
影响因子 :9.0
引用产品: RPMI 8226 细胞
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Lnc NEAT1/miR-29b-3p/Sp1 form a positive feedback loop and modulate bortezomib resistance in human multiple myeloma cells (2011-00-20)
作者:Feifei Che:1.Department of Hematology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, 610072, Sichuan, China. Electronic address: Chefeifei2@126.com. ; Xuemei Ye:1.Department of Hematology, Dongli Medical District of Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, 610051, Sichuan, China. ; Yu Wang:1.Department of Hematology, Dongli Medical District of Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, 610051, Sichuan, China. ; Shuyue Ma:1.Department of Hematology, Dongli Medical District of Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Ch
期刊:Lnc NEAT1/miR-29b-3p/Sp1 form a positive feedback loop and modulate bortezomib resistance in human multiple myeloma cells
DOI:10.1016/j.ejphar.2020.173752
影响因子 :5.0
引用产品: RPMI 8226 细胞 , U266 [U266B1] 细胞
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