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产品概述
平衡盐溶液（Balanced Salt Solution, BSS）也叫生理溶液（Physiological Solution），是集缓冲液的缓冲力、生理盐水的等渗性以及培养液的营养供应特点于一体的，具有维持渗透压、保持PH稳定以及提供简单的营养的作用，可满足体外实验中组织、器官或细胞的生存和代谢的基本需要。一些平衡盐溶液中还添加了少量酚红，用以指示溶液的酸碱度变化。
Hank's平衡盐溶液（Hank's Balanced Salt Solution, HBSS）是细胞分离或培养中常用的磷酸盐缓冲液之一，主要成分包括NaCl、KCl、KH2PO4、Na2HPO4、NaHCO3、CaCl2、MgCl2、MgSO4和葡萄糖。Hank's平衡盐溶液常用于组织块的漂洗、细胞的洗涤、细胞或组织的运输、其他试剂的配制以及作为细胞计数的稀释液等。
产品信息
形态 | 液体 |
浓度 | 1 × |
规格 | 500mL |
pH | 7.2-7.4 |
CaCl2（无水） | 140 mg/L |
MgCl2•6H2O | 100 mg/L |
MgSO4•7H2O | 100 mg/L |
NaHCO3 | 350 mg/L |
D-葡萄糖 | 1000 mg/L |
酚红指示剂 | 10 mg/L |
储存条件 | 2-30℃ |
运输条件 | 常温 |
有效期 | 36个月 |
注意事项
1、本产品为即用型，经0.1 μm过滤除菌，可直接使用，使用前无需稀释或过滤除菌等任何处理；
2、使用本产品时应注意无菌操作，避免污染；
3、由于Hank's平衡盐溶液含有钙镁离子，易产生少量沉淀，一般温浴后沉淀即可溶解；若沉淀较多且又无法溶解时，请放弃使用；
4、由于Hank's平衡盐溶液NaHCO3含量较低，不能用于5% CO2的环境，若放入CO2培养相，溶液将迅速变酸，使用时应注意；
5、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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Curculigoside Attenuates Endoplasmic Reticulum Stress-Induced Epithelial Cell and Fibroblast Senescence by Regulating the SIRT1-P300 Signaling Pathway (2024-03-29)
作者：Weixi Xie:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Lang Deng:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Rui Qian:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Xiaoting Huang:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Wei Liu:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; Siyuan Tang:1.Xiangya Nursing School, Central South University, Changsha 410013, China. ; 2.The School of Nursing, Ningxia Medical University, Yinchuan 750004, China. ;
期刊：Curculigoside Attenuates Endoplasmic Reticulum Stress-Induced Epithelial Cell and Fibroblast Senescence by Regulating the SIRT1-P300 Signaling Pathway
影响因子 ：7.0
引用产品： 青霉素-链霉素溶液 (双抗)，100× , Hank's平衡盐溶液 (HBSS)，含钙、镁，不含酚红 , HEPES液 (1M) , 特级胎牛血清
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Nilotinib in combination with sunitinib renders MCL-1 for degradation and activates autophagy that overcomes sunitinib resistance in renal cell carcinoma (2024-02-23)
作者：Tingyu Liu:1.State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China. ; Xin Yue:1.Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510080, China. yuex5@mail.sysu.edu.cn. ; Xue Chen:1.State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China. ; Ru Yan:1.State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China. ; Chong Wu:1.State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China. ; Yunzhi Li:1.School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China. ; Xianzhang
期刊：Nilotinib in combination with sunitinib renders MCL-1 for degradation and activates autophagy that overcomes sunitinib resistance in renal cell carcinoma
DOI：10.1007/s13402-024-00927-9
影响因子 ：6.6
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Intestinal Absorption of Nanoparticles to Reduce Oxidative Stress and Vasoconstriction for Treating Diabetic Nephropathy (2024-02-20)
作者：Chenxin Liu:1.School of Pharmaceutical Sciences, Zhengzhou University, 100 Science Road, Zhengzhou 450001, China. ; Mengxue Pang:1.School of Pharmaceutical Sciences, Zhengzhou University, 100 Science Road, Zhengzhou 450001, China. ; Qingyu Wang:1.School of Pharmaceutical Sciences, Zhengzhou University, 100 Science Road, Zhengzhou 450001, China. ; Mei Yan:1.School of Pharmaceutical Sciences, Zhengzhou University, 100 Science Road, Zhengzhou 450001, China. ; Yingying Zhou:1.School of Pharmaceutical Sciences, Zhengzhou University, 100 Science Road, Zhengzhou 450001, China. ; Hanchun Yao:1.School of Pharmaceutical Sciences, Zhengzhou University, 100 Science Road, Zhengzhou 450001, China. ; 2.Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases, Henan Province, 100 Science Road, Zhengzhou 450001, China. ; Bin Du:1.School of Pharmaceutical Sciences, Zhengzhou University, 100 Science Road, Zhengzhou 450001, China. ; 2.Key Laboratory of Targeting Therapy and Diagnosis
期刊：Intestinal Absorption of Nanoparticles to Reduce Oxidative Stress and Vasoconstriction for Treating Diabetic Nephropathy
DOI：10.1021/acsbiomaterials.3c01353
影响因子 ：5.8
引用产品： MEM 培养基 , Hank's平衡盐溶液 (HBSS)，含钙、镁，不含酚红
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Preparation and characterization of a novel composite acellular matrix/hyaluronic acid thermosensitive hydrogel for interstitial cystitis/bladder pain syndrome (2023-11-16)
作者：Wei Guo:1.Department of Urology, Beijing Chao-Yang Hospital, Beijing, China. ; Haichao Liu:1.Department of Urology, Hebei Yanda Hospital, West of SiPuLan Road, Langfang, China. ; Jiaxing Zhang:1.Department of Urology, Hebei Yanda Hospital, West of SiPuLan Road, Langfang, China. ; Jianzhong Zhang:1.Department of Urology, Beijing Chao-Yang Hospital, Beijing, China. ; Fei Wang:1.Department of Urology, Beijing Chao-Yang Hospital, Beijing, China. ; Peng Zhang:1.Department of Urology, Beijing Chao-Yang Hospital, Beijing, China. ; Yunbo Yang:1.Department of Urology, Hebei Yanda Hospital, West of SiPuLan Road, Langfang, China. ;
期刊：Preparation and characterization of a novel composite acellular matrix/hyaluronic acid thermosensitive hydrogel for interstitial cystitis/bladder pain syndrome
影响因子 ：4.9
引用产品： Hank's平衡盐溶液 (HBSS)，含钙、镁，不含酚红 , 磷酸盐缓冲液 (PBS) 1× , 小鼠骨髓间充质干细胞
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Perfluorooctane Sulfonate Induces Dysfunction of Human Umbilical Vein Endothelial Cells via Ferroptosis Pathway (2022-08-28)
作者：Jiajing Cui:1.Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun 130021, China. ; Pingwei Wang:1.Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun 130021, China. ; Shuqi Yan:1.Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun 130021, China. ; Yujun Liang:1.Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun 130021, China. ; Dongge Liu:1.Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun 130021, China. ; Shuping Ren:1.Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun 130021, China. ;
期刊：Perfluorooctane Sulfonate Induces Dysfunction of Human Umbilical Vein Endothelial Cells via Ferroptosis Pathway
影响因子 ：4.5
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