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产品概述
HEPES全称为N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid，中文名称为4-羟乙基哌嗪乙磺酸，是一种常用的生物缓冲剂，pKa (25℃)=7.5，有效缓冲范围为pH 6.8-8.2。HEPES最大的优点是，能够在开放环境中较长时间恒定溶液的PH范围。HEPES不能通过生物膜，对生化反应影响有限，是一种化学和酶稳定的物质，而且HEPES对可见光和紫外光的吸收度极低。因此一般认为，HEPES是一种优良的生物缓冲剂，对细胞无毒性作用，使用终浓度为10-50 mM，细胞培养常用10 mM。
产品信息
形态 | 液体 |
浓度 | 1 M |
规格 | 100mL |
PH | 7.2-7.4 |
溶剂 | 纯化水 |
HEPES | 238.3 g/L |
储存条件 | 2-8℃或-5~-20℃，避光。 |
运输条件 | 常温 |
有效期 | 2-8℃，24个月；-5~-20℃，长期。 |
注意事项
1、本产品经0.1 μm过滤除菌；
2、使用本产品时应注意无菌操作，避免污染；
3、使用后请及时避光保存，有研究报道培养液中的HEPES在可见光下暴露3h以上将产生对细胞有毒性的过氧化氢；
4、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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Curculigoside Attenuates Endoplasmic Reticulum Stress-Induced Epithelial Cell and Fibroblast Senescence by Regulating the SIRT1-P300 Signaling Pathway (2024-03-29)
期刊：Antioxidants
影响因子 ：7.0
引用产品： 青霉素-链霉素溶液 (双抗)，100× , Hank's平衡盐溶液 (HBSS)，含钙、镁，不含酚红 , HEPES液 (1M) , 特级胎牛血清
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FOXM1 Participates in Scleral Remodeling in Myopia by Upregulating APOA1 Expression Through METTL3/YTHDF2 (2024-01-02)
期刊：INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
影响因子 ：4.4
引用产品： 人巩膜成纤维细胞 , HEPES液 (1M)
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LncRNA HOTTIP regulates TLR4 promoter methylation by recruiting H3K4 methyltransferase MLL1 to affect apoptosis and inflammatory response of fibroblast-like synoviocyte in rheumatoid arthritis (2024-02-16)
期刊：KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
影响因子 ：3.3
引用产品： HEPES液 (1M)
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Remodeling Collagen Microenvironment in Liver Using a Biomimetic Nano-Regulator for Reversal of Liver Fibrosis (2023-04-23)
期刊：Advanced Science
影响因子 ：15.1
引用产品： DMEM/F12 培养基 , HEPES液 (1M) , AML-12细胞专用培养基 , RPMI-1640 培养基
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Role of macrophage AHR/TLR4/STAT3 signaling axis in the colitis induced by non-canonical AHR ligand aflatoxin B1 (2023-03-23)
期刊：JOURNAL OF HAZARDOUS MATERIALS
DOI：10.1016/j.jhazmat.2023.131262
影响因子 ：13.6
引用产品： RPMI-1640 培养基 , L-谷氨酰胺溶液 (200mM)，100× , β-巯基乙醇 (55mM)，1000× , HEPES液 (1M) , MEM非必需氨基酸溶液 (NEAA)，100×
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miR-143-3p shuttled by M2 macrophage-derived extracellular vesicles induces progression of colorectal cancer through a ZC3H12A/C/EBPβ axis-dependent mechanism (2023-04-29)
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2023.110137
影响因子 ：5.6
引用产品： HEPES液 (1M)
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Force-Regulated Calcium Signaling of Lymphoid Cell RPMI 8226 Mediated by Integrin α4β7/MAdCAM-1 in Flow (2023-03-24)
期刊：Biomolecules
影响因子 ：5.5
引用产品： RPMI-1640 培养基 , 青霉素-链霉素溶液 (双抗)，100× , HEPES液 (1M)
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Novel nano-micro-macro multiple-nested hydrogel with gradient ciliary neurotrophic factor distribution induces directional axon regeneration of retinal ganglion cells (2023-06-28)
期刊：COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
DOI：10.1016/j.colsurfa.2023.131904
影响因子 ：5.2
引用产品： HEPES液 (1M)
-
METTL3-mediated m6A modification of pri-miR-148a-3p affects prostate cancer progression by regulating TXNIP (2023-07-14)
期刊：ENVIRONMENTAL TOXICOLOGY
影响因子 ：4.5
引用产品： HEPES液 (1M)
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microRNA-651-5p affects the proliferation, migration, and invasion of lung cancer cells by regulating Calmodulin 2 expression (2023-07-20)
期刊：Clinical Respiratory Journal
影响因子 ：1.7
引用产品： HEPES液 (1M)
-
The exploration of quantum dot-molecular beacon based MoS2 fluorescence probing for myeloma-related Mirnas detection (2001-02-20)
期刊：Bioactive Materials
DOI：10.1016/j.bioactmat.2021.12.036
影响因子 ：18.9
引用产品： HEPES液 (1M)
-
Environmental Factors and Their Threshold Affecting the Survival of Five Aquatic Animal Viruses in Different Animal Cells (2022-11-17)
期刊：Viruses-Basel
影响因子 ：5.8
引用产品： HEPES液 (1M)
-
Long non-coding RNA tumor protein 53 target gene 1 promotes cervical cancer development via regulating microRNA-33a-5p to target forkhead box K2 (2001-02-20)
期刊：Cell Cycle
DOI：10.1080/15384101.2022.2026705
影响因子 ：5.2
引用产品： HEPES液 (1M)
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Oncogenic magnesium transporter 1 upregulates programmed death-1-ligand 1 expression and contributes to growth and radioresistance of glioma cells through the ERK/MAPK signaling pathway (2004-02-20)
期刊：Bioengineered
DOI：10.1080/21655979.2022.2037214
影响因子 ：4.9
引用产品： HEPES液 (1M)
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LncRNA NEAT1 Inhibits Neuronal Apoptosis and Induces Neuronal Viability of Depressed Rats Via microRNA-320-3p/CRHR1 Axis (2001-02-20)
期刊：NEUROCHEMICAL RESEARCH
DOI：10.1007/s11064-021-03508-6
影响因子 ：4.4
引用产品： HEPES液 (1M)
-
miR-339-3p inhibits cell growth and epithelial–mesenchymal transition in nasopharyngeal carcinoma by modulating the KAT6A/TRIM24 axis (2022-08-17)
期刊：International Journal of Clinical Oncology
DOI：10.1007/s10147-022-02231-8
影响因子 ：3.9
引用产品： HEPES液 (1M)
-
Effects of total glucosides of paeony on acute renal injury following ischemia-reperfusion via the lncRNA HCG18/miR-16-5p/Bcl-2 axis (2001-02-20)
期刊：Immunobiology
DOI：10.1016/j.imbio.2022.152179
影响因子 ：2.8
引用产品： HEPES液 (1M)
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microRNA-186 in extracellular vesicles from bone marrow mesenchymal stem cells alleviates idiopathic pulmonary fibrosis via interaction with SOX4 and DKK1 (2002-01-20)
期刊：Stem Cell Research & Therapy
DOI：10.1186/s13287-020-02083-x
影响因子 ：8.1
引用产品： HEPES液 (1M)
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LncRNA SNHG6 promotes G1/S-phase transition in hepatocellular carcinoma by impairing miR-204-5p-mediated inhibition of E2F1 (2004-01-20)
期刊：Oncogene
DOI：10.1038/s41388-021-01671-2
影响因子 ：8.0
引用产品： HEPES液 (1M)
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Reduction of miR-744 delivered by NSCLC cell-derived extracellular vesicles upregulates SUV39H1 to promote NSCLC progression via activation of the Smad9/BMP9 axis (2001-01-20)
期刊：Journal of Translational Medicine
DOI：10.1186/s12967-020-02654-9
影响因子 ：7.4
引用产品： HEPES液 (1M)
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Dexmedetomidine inhibits mitochondria damage and apoptosis of enteric glial cells in experimental intestinal ischemia/reperfusion injury via SIRT3-dependent PINK1/HDAC3/p53 pathway (2011-01-20)
期刊：Journal of Translational Medicine
DOI：10.1186/s12967-021-03027-6
影响因子 ：7.4
引用产品： HEPES液 (1M)
-
microRNA-363-3p reduces endothelial cell inflammatory responses in coronary heart disease via inactivation of the NOX4-dependent p38 MAPK axis (2003-01-20)
期刊：Aging-US
影响因子 ：5.2
引用产品： HEPES液 (1M)
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DNMT3B decreases extracellular matrix degradation and alleviates intervertebral disc degeneration through TRPA1 methylation to inhibit the COX2/YAP axis (2008-01-20)
期刊：Aging-US
影响因子 ：5.2
引用产品： HEPES液 (1M)
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NLRC4 gene silencing-dependent blockade of NOD-like receptor pathway inhibits inflammation, reduces proliferation and increases apoptosis of dendritic cells in mice with septic shock (2001-01-20)
期刊：Aging-US
影响因子 ：5.2
引用产品： HEPES液 (1M)
-
Dexmedetomidine Ameliorates Postoperative Cognitive Dysfunction via the MicroRNA-381-Mediated EGR1/p53 Axis (2007-01-20)
期刊：Molecular Neurobiology
DOI：10.1007/s12035-021-02417-7
影响因子 ：5.1
引用产品： HEPES液 (1M)
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Krüppel-Like Factor 6 Silencing Prevents Oxidative Stress and Neurological Dysfunction Following Intracerebral Hemorrhage via Sirtuin 5/Nrf2/HO-1 Axis (2006-01-20)
期刊：Frontiers in Aging Neuroscience
影响因子 ：4.8
引用产品： HEPES液 (1M)
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Silenced lncRNA H19 and up-regulated microRNA-129 accelerates viability and restrains apoptosis of PC12 cells induced by Aβ25-35 in a cellular model of Alzheimer’s disease (2001-01-20)
期刊：Cell Cycle
DOI：10.1080/15384101.2020.1863681
影响因子 ：4.3
引用产品： HEPES液 (1M)
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Silencing Long Non-coding RNA Kcnq1ot1 Limits Acute Kidney Injury by Promoting miR-204-5p and Blocking the Activation of NLRP3 Inflammasome (2011-01-20)
期刊：Frontiers in Physiology
影响因子 ：4.0
引用产品： HEPES液 (1M)
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Histone Deacetylase Sirtuin 2 Enhances Viability of Trophoblasts Through p65-Mediated MicroRNA-146a/ACKR2 Axis (2001-01-20)
期刊：Reproductive Sciences
DOI：10.1007/s43032-020-00398-x
影响因子 ：2.9
引用产品： HEPES液 (1M)
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Silencing LINC00294 Restores Mitochondrial Function and Inhibits Apoptosis of Glioma Cells under Hypoxia via the miR-21-5p/CASKIN1/cAMP Axis (2011-01-20)
期刊：Oxidative Medicine and Cellular Longevity
影响因子 ：0.0
引用产品： HEPES液 (1M)
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Oncogenic Role of Long Noncoding RNAMALAT1 in Thyroid Cancer Progression through Regulation of the miR-204/IGF2BP2/m6A-MYC Signaling (2009-00-20)
期刊：Molecular Therapy-Nucleic Acids
DOI：10.1016/j.omtn.2020.09.023
影响因子 ：8.8
引用产品： HEPES液 (1M)
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MCM3AP-AS1 KD Inhibits Proliferation, Invasion, and Migration of PCa Cells via DNMT1/DNMT3 (A/B) Methylation-Mediated Upregulation of NPY1R (2001-00-20)
期刊：Molecular Therapy-Nucleic Acids
DOI：10.1016/j.omtn.2020.01.016
影响因子 ：8.8
引用产品： HEPES液 (1M)
-
MicroRNA-381 facilitates autophagy and apoptosis in prostate cancer cells via inhibiting the RELN-mediated PI3K/AKT/mTOR signaling pathway (2004-00-20)
期刊：Life Sciences
影响因子 ：6.8
引用产品： HEPES液 (1M)
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microRNA-9-5p regulates the mitochondrial function of hepatocellular carcinoma cells through suppressing PDK4 (2011-00-20)
期刊：Cancer Gene Therapy
DOI：10.1038/s41417-020-00253-w
影响因子 ：6.4
引用产品： HEPES液 (1M)
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HBXIP promotes gastric cancer via METTL3-mediated MYC mRNA m6A modification (2010-00-20)
期刊：Aging-US
影响因子 ：5.2
引用产品： HEPES液 (1M)
-
Inhibition of microRNA-300 inhibits cell adhesion, migration, and invasion of prostate cancer cells by promoting the expression of DAB1 (2010-00-20)
期刊：Cell Cycle
DOI：10.1080/15384101.2020.1823730
影响因子 ：4.3
引用产品： HEPES液 (1M)
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Inhibition of MicroRNA-302c on Stemness of Colon Cancer Stem Cells via the CARF/Wnt/β-Catenin Axis (2007-00-20)
期刊：Digestive Diseases and Sciences
DOI：10.1007/s10620-020-06435-8
影响因子 ：3.1
引用产品： HEPES液 (1M)
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MicroRNA‐377 exerts a potent suppressive role in osteosarcoma through the involvement of the histone acetyltransferase 1‐mediated Wnt axis (2005-09-20)
期刊：Journal Of Cellular Physiology
影响因子 ：5.6
引用产品： HEPES液 (1M)
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Up-regulation of THY1 attenuates interstitial pulmonary fibrosis and promotes lung fibroblast apoptosis during acute interstitial pneumonia by blockade of the WNT signaling pathway (2019-03-04)
期刊：Cell Cycle
DOI：10.1080/15384101.2019.1578144
影响因子 ：4.3
引用产品： HEPES液 (1M)
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Over-expression of JAZF1 promotes cardiac microvascular endothelial cell proliferation and angiogenesis via activation of the Akt signaling pathway in rats with myocardial ischemia-reperfusion (2006-09-20)
期刊：Cell Cycle
DOI：10.1080/15384101.2019.1629774
影响因子 ：4.3
引用产品： HEPES液 (1M)
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Overexpression of miR-150 Inhibits the NF-κB Signal Pathway in Intervertebral Disc Degeneration through Targeting P2X7 (2011-09-20)
期刊：Cells Tissues Organs
影响因子 ：2.7
引用产品： HEPES液 (1M)
-
Inhibitory effect of MSH6 gene silencing in combination with cisplatin on cell proliferation of human osteosarcoma cell line MG63 (2011-08-20)
期刊：Journal Of Cellular Physiology
影响因子 ：5.6
引用产品： HEPES液 (1M)
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Inhibition of microRNA‐129‐5p expression ameliorates ultraviolet ray‐induced corneal epithelial cell injury via upregulation of EGFR (2012-08-20)
期刊：Journal Of Cellular Physiology
影响因子 ：5.6
引用产品： HEPES液 (1M)
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MicroRNA-488 inhibits progression of colorectal cancer via inhibition of the mitogen-activated protein kinase pathway by targeting claudin-2 (2009-08-20)
期刊：American Journal Of Physiology-Cell Physiology
DOI：10.1152/ajpcell.00047.2018
影响因子 ：5.5
引用产品： HEPES液 (1M)
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Specific knockdown of HOXB7 inhibits cutaneous squamous cell carcinoma cell migration and invasion while inducing apoptosis via the Wnt/β-catenin signaling pathway (2011-08-20)
期刊：American Journal of Physiology-Cell Physiology
DOI：10.1152/ajpcell.00291.2017
影响因子 ：5.5
引用产品： HEPES液 (1M)
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Retracted: S100A9 gene silencing inhibits the release of pro‐inflammatory cytokines by blocking the IL‐17 signalling pathway in mice with acute pancreatitis (2002-08-20)
期刊：Journal Of Cellular And Molecular Medicine
影响因子 ：5.3
引用产品： HEPES液 (1M)
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MicroRNA-23a inhibits melanoma cell proliferation, migration, and invasion in mice through a negative feedback regulation of sdcbp and the MAPK/ERK signaling pathway (2012-08-20)
期刊：Iubmb Life
DOI：10.1002/iub.1979
影响因子 ：4.6
引用产品： HEPES液 (1M)
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Retracted: MicroRNA-381-induced down-regulation of CXCR4 promotes the proliferation of renal tubular epithelial cells in rat models of renal ischemia reperfusion injury (2001-08-20)
期刊：Journal Of Cellular Biochemistry
影响因子 ：4.0
引用产品： HEPES液 (1M)
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Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice (2008-07-20)
期刊：Bioscience Reports
影响因子 ：4.0
引用产品： HEPES液 (1M)
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MicroRNA-182 downregulates Wnt/β-catenin signaling, inhibits proliferation, and promotes apoptosis in human osteosarcoma cells by targeting HOXA9 (2009-07-20)
期刊：Oncotarget
影响因子 ：0.0
引用产品： HEPES液 (1M)
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