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产品概述
名称 | SK-N-SH (人神经母细胞瘤细胞) (STR鉴定正确) |
别称 | SK N SH; SKN-SH; SK-NSH; SKNSH; NSH |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
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推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | SK-N-SH细胞是由J·L·Bieder建系,它与SK-N-MC细胞所不同的是倍增时间较长,且多巴胺-β-羟基酶水平较高。SK-N-SH细胞在细胞介导的细胞毒性试验中用作靶细胞系。 |
年龄(性别) | 女性;4岁 |
组织来源 | 脑神经母细胞瘤,转移部位骨髓 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 神经母细胞瘤细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~44 hours |
抗原表达情况 | Blood Type A; Rh+ |
基因表达情况 | plasminogen activator, shows increased expression of M-CSF after treatment with amyloid-beta peptide. |
保藏机构 | ATCC; HTB-11 ECACC; 86012802 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11 D2S1338 17,19 D3S1358 15,16 D5S818 12 D7S820 7,10 D8S1179 15 D13S317 11 D16S539 8,13 D18S51 13,16 D19S433 13,14 D21S11 31,31.2 FGA 23.2,24 PentaD 10,12 PentaE 7,11 TH01 7,10 TPOX 8,11 vWA 14,18 D6S1043 12,18 D12S391 18,22 D2S441 11,11.3 -
STR鉴定图
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参考文献
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Resveratrol alleviates amyloid β-induced neuronal apoptosis, inflammation, and oxidative and endoplasmic reticulum stress by circ_0050263/miR-361-3p/PDE4A axis during Alzheimer's disease (2023-08-24)
期刊:Chemical Biology & Drug Design
影响因子 :3.0
引用产品: SK-N-SH 细胞 , DMEM高糖 培养基 , 特级胎牛血清
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Sevoflurane induced neurotoxicity in neonatal mice links to a GSK3β/Drp1-dependent mitochondrial fission and apoptosis (2002-02-20)
期刊:FREE RADICAL BIOLOGY AND MEDICINE
DOI:10.1016/j.freeradbiomed.2022.01.031
影响因子 :7.4
引用产品: SK-N-SH 细胞 , 293T [HEK-293T] 细胞
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C-terminal truncation modulates α-Synuclein’s cytotoxicity and aggregation by promoting the interactions with membrane and chaperone (2022-08-09)
期刊:Communications Biology
DOI:10.1038/s42003-022-03768-0
影响因子 :6.5
引用产品: SK-N-SH 细胞
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Circ_0000647 promotes cell injury by modulating miR-126-5p/TRAF3 axis in oxygen-glucose deprivation and reperfusion-induced SK-N-SH cell model (2001-02-20)
期刊:International Immunopharmacology
DOI:10.1016/j.intimp.2021.108464
影响因子 :5.6
引用产品: SK-N-SH 细胞 , SK-N-SH细胞专用培养基
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Silencing of long chain noncoding RNA paternally expressed gene (PEG10) inhibits the progression of neuroblastoma by regulating microRNA-449a (miR-449a)/ribosomal protein S2 (RPS2) axis (2002-02-20)
期刊:Bioengineered
DOI:10.1080/21655979.2022.2042999
影响因子 :4.9
引用产品: 人脐静脉内皮细胞 , SH-SY5Y [SHSY-5Y] 细胞 , SK-N-SH 细胞 , SK-N-AS 细胞 , SK-N-BE (2) 细胞
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CDC27-ODC1 Axis Promotes Metastasis, Accelerates Ferroptosis and Predicts Poor Prognosis in Neuroblastoma (2002-02-20)
期刊:Frontiers in Oncology
影响因子 :4.7
引用产品: SK-N-SH 细胞 , SH-SY5Y [SHSY-5Y] 细胞
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CircAXL Knockdown Alleviates Aβ1-42-Induced Neurotoxicity in Alzheimer’s Disease via Repressing PDE4A by Releasing miR-1306-5p (2003-02-20)
期刊:Neurochemical Research
DOI:10.1007/s11064-022-03563-7
影响因子 :4.4
引用产品: SK-N-SH 细胞
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Long non-coding RNA UCA1 regulates MPP+-induced neuronal damage through the miR-671-5p/KPNA4 pathway in SK-N-SH cells (2022-12-14)
期刊:METABOLIC BRAIN DISEASE
DOI:10.1007/s11011-022-01118-x
影响因子 :3.7
引用产品: SK-N-SH 细胞 , SK-N-SH细胞专用培养基
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HMGB3 is Associated With an Unfavorable Prognosis of Neuroblastoma and Promotes Tumor Progression by Mediating TPX2 (2012-01-20)
期刊:Frontiers in Cell and Developmental Biology
影响因子 :5.5
引用产品: SK-N-SH 细胞 , SH-SY5Y [SHSY-5Y] 细胞 , SK-N-BE (2) 细胞
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LINC00839 Regulates Proliferation, Migration, Invasion, Apoptosis and Glycolysis in Neuroblastoma Cells Through miR-338-3p/GLUT1 Axis (2006-01-20)
期刊:Neuropsychiatric Disease and Treatment
影响因子 :3.2
引用产品: IMR-32 细胞 , SK-N-SH 细胞 , 293 [HEK-293] 细胞 , DMEM高糖 培养基
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Long Noncoding RNA SNHG1 Knockdown Ameliorates Apoptosis, Oxidative Stress and Inflammation in Models of Parkinson’s Disease by Inhibiting the miR-125b-5p/MAPK1 Axis (2004-01-20)
期刊:Neuropsychiatric Disease and Treatment
影响因子 :3.2
引用产品: SK-N-SH 细胞 , 小鼠多巴胺神经元细胞
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miR-338-3p inhibits cell growth, invasion, and EMT process in neuroblastoma through targeting MMP-2 (2001-01-20)
期刊:Open Life Sciences
影响因子 :2.2
引用产品: SK-N-SH 细胞
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Silencing of long non-coding RNA DLX6-AS1 weakens neuroblastoma progression by the miR‐513c‐5p/PLK4 axis (2010-00-20)
期刊:Iubmb Life
DOI:10.1002/iub.2392
影响因子 :4.6
引用产品: SK-N-SH 细胞 , SK-N-AS 细胞
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LncRNA NEAT1 Regulates the Development of Parkinson’s Disease by Targeting AXIN1 Via Sponging miR-212-3p (2011-00-20)
期刊:Neurochemical Research
DOI:10.1007/s11064-020-03157-1
影响因子 :4.4
引用产品: SK-N-SH 细胞 , 293T [HEK-293T] 细胞 , 青霉素-链霉素溶液 (双抗),100×
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