产品中心Cell Resources
联系我们CONTACT US
400-999-210024小时服务热线
产品概述
| 名称 | SK-N-BE(2) (人神经母细胞瘤细胞) (STR鉴定正确) |
| 别称 | SK-N-BE2; SKNBE(2); SKNBE-2; SKNBE2; SK-N-BE; SKNBE |
| 种属 | 人 |
| 生长特性 | 半贴半悬 |
| 细胞形态 | 神经母细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | SK-N-BE(2)神经母细胞瘤细胞株是于1972年11月从一位多次化疗及放疗的扩散性神经母细胞瘤患儿骨髓穿刺物中建立的；SK-N-BE(2)细胞显示中等水平的多巴胺-β-羟基酶活性。有报道称，SK-N-BE(2)细胞的饱和浓度超过1×10^6细胞/cm^2。SK-N-BE(2)细胞形态多样，有的有长突触、有的呈上皮细胞样；SK-N-BE(2)细胞会聚集，形成团块并浮起。 |
| 年龄（性别） | 男性；2岁 |
| 组织来源 | 脑；神经母细胞瘤，骨髓转移灶 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 神经母细胞瘤细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~36-48 hours |
| 致瘤性 | Yes, an inoculum of 1×10^7 cells produced tumors in cortisonized hamster cheek pouches with 18% frequency. |
| 保藏机构 | ATCC; CRL-2271 DSMZ; ACC-632 ECACC; 95011815 |
STR鉴定
-
STR位点信息
Amelogenin X,Y CSF1PO 10 D2S1338 17,23 D3S1358 19 D5S818 12 D7S820 9,10 D8S1179 13,14 D13S317 11 D16S539 9,11 D18S51 16 D19S433 12,13 D21S11 30,32.2 FGA 22,25 PentaD 13,14 PentaE 14,18 TH01 6,7 TPOX 8,11 vWA 18 D6S1043 11,19 D12S391 18,24 D2S441 11,14 -
STR鉴定图
-
参考文献
-
USP29 activation mediated by FUBP1 promotes AURKB stability and oncogenic functions in gastric cancer (2024-01-17)
作者：Rongfu Tu:1.Department of Cancer Precision Medicine, The MED-X Institute, The First Affiliated Hospital of Xi'an Jiaotong University, Building 21, Xi'an, China. rongfutu@xjtu.edu.cn. ; Ye Kang:1.Department of Cancer Precision Medicine, The MED-X Institute, The First Affiliated Hospital of Xi'an Jiaotong University, Building 21, Xi'an, China. ; Yiwen Pan:1.Precision Medicine Center, The First Affiliated Hospital of Xi'an Jiaotong University, 277 Yanta West Road, Xi'an, 710061, China. ; Yanyan Da:1.Center for Molecular Diagnosis and Precision Medicine, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, 1519 Dongyue Dadao, Nanchang, 330209, China. ; 2.Department of Clinical Laboratory, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, 17 Yongwai Zhengjie, Nanchang, 330006, China. ; 3.Jiangxi Provincial Center for Advanced Diagnostic Technology and Precision Medicine, The First Affiliated Hospital, Jiangxi Medical College, Nanchang Un
期刊：USP29 activation mediated by FUBP1 promotes AURKB stability and oncogenic functions in gastric cancer
DOI：10.1186/s12935-024-03224-5
影响因子 ：5.8
引用产品： HGC-27 细胞 , SNU-216 细胞 , HCT 116 细胞 , A549 [A-549] 细胞 , SK-N-BE (2) 细胞 , 293T [HEK-293T] 细胞
-
Potential Cause-and-Effect Relationship between Gut Microbiota and Childhood Neuroblastoma: A Mendelian Randomization Analysis (2024-03-27)
作者：Jing Chu:1.Department of Pathology, Anhui Provincial Children's Hospital, 39 Wangjiang East Road, Hefei, Anhui, 230051, China. chujing198603@163.com. ;
期刊：Potential Cause-and-Effect Relationship between Gut Microbiota and Childhood Neuroblastoma: A Mendelian Randomization Analysis
DOI：10.1007/s12098-024-05065-6
影响因子 ：4.3
引用产品： SK-N-BE (2) 细胞 , SH-SY5Y [SHSY-5Y] 细胞
-
Enhancing the sensitization of neuroblastoma to radiotherapy by the construction of a dual-channel parallel free radicals nanoamplifier (2023-10-03)
作者：Wenxin Zhang:1.Department of Pediatric Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, 510282, China. ; Xiaodie Li:1.Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, 510282, China. ; Jialin Zeng:1.Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, 510282, China. ; Xin Wen:1.Clinical Research Centre, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China. ; Chao Zhang:1.Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, 510282, China. ; Yinan Zhang:1.School of Chemical Science and Engineering, Tongji University, Shanghai, 200092, China. ; Jian He:1.Department of Nuclear Medicine, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, 210008, China. ; Lihua Yang:1.Department of Pediatric Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou,
期刊：Enhancing the sensitization of neuroblastoma to radiotherapy by the construction of a dual-channel parallel free radicals nanoamplifier
DOI：10.1016/j.mtbio.2023.100828
影响因子 ：8.2
引用产品： DMEM高糖 培养基 , 磷酸盐缓冲液 (PBS) 1× , SK-N-BE (2) 细胞
-
Mechanism of Endogenous Peptide PDYBX1 and Precursor Protein YBX1 in Hirschsprung’s Disease (2023-10-01)
作者：Qiaochu Sun:1.Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, 210008, China. ; Zhengke Zhi:1.Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, 210008, China. ; Chenglong Wang:1.Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, 210008, China. ; Chunxia Du:1.Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, 210008, China. ; Jie Tang:1.Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, 210008, China. ; Hongxing Li:1.Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, 210008, China. hx8817@njmu.edu.cn. ; Weibing Tang:1.Department of Pediatric Surgery, Children's Hospital of Nanjing Medical University, Nanjing, 210008, China. twbcn@njmu.edu.cn. ;
期刊：Mechanism of Endogenous Peptide PDYBX1 and Precursor Protein YBX1 in Hirschsprung’s Disease
DOI：10.1007/s12264-023-01132-8
影响因子 ：5.6
引用产品： SK-N-BE (2) 细胞 , 特级胎牛血清 , 青霉素-链霉素-两性霉素B溶液 (100×) , 小鼠肠神经嵴干细胞完全培养基
-
Silencing of long chain noncoding RNA paternally expressed gene (PEG10) inhibits the progression of neuroblastoma by regulating microRNA-449a (miR-449a)/ribosomal protein S2 (RPS2) axis (2002-02-20)
作者：Jian Zhang:1.Department of Geriatrics, Qingdao Chengyang District People's Hospital, Qingdao, Shandong, P.R. China. ; Wei Liu:1.Department of Health Management, Qingdao Eighth People's Hospital, Qingdao, Shandong, P.R. China. ; Ping Ji:1.Department of Ophthalmology, Qingdao Eighth People's Hospital, Qingdao, Shandong, P.R. China. ; Yan Zhang:1.The Third Department of Internal Medicine, Qingdao Women and Children's Hospital, Qingdao University, Qingdao, Shandong, P.R. China. ;
期刊：Silencing of long chain noncoding RNA paternally expressed gene (PEG10) inhibits the progression of neuroblastoma by regulating microRNA-449a (miR-449a)/ribosomal protein S2 (RPS2) axis
DOI：10.1080/21655979.2022.2042999
影响因子 ：4.9
引用产品： 人脐静脉内皮细胞 , SH-SY5Y [SHSY-5Y] 细胞 , SK-N-SH 细胞 , SK-N-AS 细胞 , SK-N-BE (2) 细胞
-
HMGB3 is Associated With an Unfavorable Prognosis of Neuroblastoma and Promotes Tumor Progression by Mediating TPX2 (2012-01-20)
作者：Xiaodan Zhong:1.Department of Pediatric Oncology, The First Hospital of Jilin University, Changchun, China. ; Songling Zhang:1.Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun, China. ; Yutong Zhang:1.Department of Pediatric Oncology, The First Hospital of Jilin University, Changchun, China. ; Zongmiao Jiang:1.Department of Endocrinology and Metabolism, The First Hospital of Jilin University, Changchun, China. ; Yanan Li:1.Department of Pediatrics, The First Hospital of Jilin University, Changchun, China. ; Jian Chang:1.Department of Pediatric Oncology, The First Hospital of Jilin University, Changchun, China. ; Junqi Niu:1.Department of Hepatology, The First Hospital of Jilin University, Changchun, China. ; Ying Shi:1.Department of Hepatology, The First Hospital of Jilin University, Changchun, China. ;
期刊：HMGB3 is Associated With an Unfavorable Prognosis of Neuroblastoma and Promotes Tumor Progression by Mediating TPX2
影响因子 ：5.5
引用产品： SK-N-SH 细胞 , SH-SY5Y [SHSY-5Y] 细胞 , SK-N-BE (2) 细胞
FAQs
Q：{{item.question}}
A：
产品资料
识别码示意图
